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The map label for this gene is panB

Identifier: 103487687

GI number: 103487687

Start: 2318435

End: 2319298

Strand: Reverse

Name: panB

Synonym: Sala_2206

Alternate gene names: 103487687

Gene position: 2319298-2318435 (Counterclockwise)

Preceding gene: 103487690

Following gene: 103487686

Centisome position: 69.33

GC content: 64.93

Gene sequence:

>864_bases
ATGTCTACGCTCCCCAAAACGCTCACCCTCGACACCTCCACGAGCCGCGCAAATCCGACGCCGCAGCCGATGAAGCGCCT
GACGGTTCCGCGCATCCGCCAGCGCAAGGGGGGCGAACCGATCGTGATGCTCACCGCCTATACGGTGCGCATGGCGCAGT
TGCTCGACCCGCATTGCGACATGCTGCTGGTGGGCGATTCGCTCGCGCAGGTGATCTATGGCCTGCCGCACACCGTCGGG
GTGACGATGGAGATGATGGCGCTGCACGGCGCCGCCGTGGTGCGCGGCAGTTACCATGCCGCGGTGATCGTCGACATGCC
CTTCGGCAGCTATGAGGGCAGCCCGCAGCAGGCCTTTGACAACGCCGCGCGACTGCTCAAGGAAACCGGCGCCGCGGCGG
TCAAGGTCGAGGGTGGCAAGGTGCTTGCTCCGACGATCGAGTTCCTGACCCAGCGCGGCATTCCGGTGATGGGGCATGTC
GGGCTGACCCCGCAGGCGGTGAACATCCTTGGCGGTTATGGCGTGCGCGGCAAGAGCGAGGAGGAAGCGCGCTCGATCGT
CGAGGATGCGGTTGCGGTCGCCCAGGCCGGCGCCTTTTCGATCGTCATCGAGGGCGTACTCGAATCGATCGCGATCGAAA
TCACCAACAAGGTCGCATGTCCGACGATCGGCATCGGCGCATCGGCGCAGTGCGATGGCCAGGTGCTCGTCACCGACGAC
ATGCTGGGCATGTTCGAGCGTGTACCCAAGTTCGTCAAACGCTATCGGGACATGGCGGGCGTCGTCAGCGGCGCCGTGCA
GGATTATGCGGACGAAGTCAGGTCCCGTTCATTCCCGACCGAGGATCAGATCTACGCGGGCTGA

Upstream 100 bases:

>100_bases
AACGTAATGTGCATTATATTTAACATTAAGTCAAGTGTCATTGACATCGAGTGGCTTTGCCGCTTTCGCCACGGTGCGTT
ACCGGCTAAGCCCTCGCGCC

Downstream 100 bases:

>100_bases
ACGGCCCGCGCGTCCGACAAGTGAAACGCTTGGCCTTTCCTCGTGTCATCGTTAAGGAAGCGGGCCGGCCTCGCGCCGTT
ACCCAGTTTACGGAGGTTTG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSTLPKTLTLDTSTSRANPTPQPMKRLTVPRIRQRKGGEPIVMLTAYTVRMAQLLDPHCDMLLVGDSLAQVIYGLPHTVG
VTMEMMALHGAAVVRGSYHAAVIVDMPFGSYEGSPQQAFDNAARLLKETGAAAVKVEGGKVLAPTIEFLTQRGIPVMGHV
GLTPQAVNILGGYGVRGKSEEEARSIVEDAVAVAQAGAFSIVIEGVLESIAIEITNKVACPTIGIGASAQCDGQVLVTDD
MLGMFERVPKFVKRYRDMAGVVSGAVQDYADEVRSRSFPTEDQIYAG

Sequences:

>Translated_287_residues
MSTLPKTLTLDTSTSRANPTPQPMKRLTVPRIRQRKGGEPIVMLTAYTVRMAQLLDPHCDMLLVGDSLAQVIYGLPHTVG
VTMEMMALHGAAVVRGSYHAAVIVDMPFGSYEGSPQQAFDNAARLLKETGAAAVKVEGGKVLAPTIEFLTQRGIPVMGHV
GLTPQAVNILGGYGVRGKSEEEARSIVEDAVAVAQAGAFSIVIEGVLESIAIEITNKVACPTIGIGASAQCDGQVLVTDD
MLGMFERVPKFVKRYRDMAGVVSGAVQDYADEVRSRSFPTEDQIYAG
>Mature_286_residues
STLPKTLTLDTSTSRANPTPQPMKRLTVPRIRQRKGGEPIVMLTAYTVRMAQLLDPHCDMLLVGDSLAQVIYGLPHTVGV
TMEMMALHGAAVVRGSYHAAVIVDMPFGSYEGSPQQAFDNAARLLKETGAAAVKVEGGKVLAPTIEFLTQRGIPVMGHVG
LTPQAVNILGGYGVRGKSEEEARSIVEDAVAVAQAGAFSIVIEGVLESIAIEITNKVACPTIGIGASAQCDGQVLVTDDM
LGMFERVPKFVKRYRDMAGVVSGAVQDYADEVRSRSFPTEDQIYAG

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=265, Percent_Identity=40.377358490566, Blast_Score=184, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6319653, Length=281, Percent_Identity=40.2135231316726, Blast_Score=182, Evalue=7e-47,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_SPHAL (Q1GR07)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617248.1
- ProteinModelPortal:   Q1GR07
- SMR:   Q1GR07
- GeneID:   4080164
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2206
- NMPDR:   fig|317655.9.peg.2135
- HOGENOM:   HBG299908
- OMA:   DMMIAHG
- ProtClustDB:   PRK00311
- BioCyc:   SALA317655:SALA_2206-MONOMER
- BRENDA:   2.1.2.11
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 30576; Mature: 30445

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: ACT_SITE 204-204 BINDING 105-105 BINDING 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLPKTLTLDTSTSRANPTPQPMKRLTVPRIRQRKGGEPIVMLTAYTVRMAQLLDPHCD
CCCCCCEEEEECCCCCCCCCHHHHHHCCCHHHHHHCCCCCEEEEHHHHHHHHHHHCCCCC
MLLVGDSLAQVIYGLPHTVGVTMEMMALHGAAVVRGSYHAAVIVDMPFGSYEGSPQQAFD
EEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCCCCCCHHHHHH
NAARLLKETGAAAVKVEGGKVLAPTIEFLTQRGIPVMGHVGLTPQAVNILGGYGVRGKSE
HHHHHHHHCCCEEEEECCCEEEHHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCCCCCCH
EEARSIVEDAVAVAQAGAFSIVIEGVLESIAIEITNKVACPTIGIGASAQCDGQVLVTDD
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCEEEEEHH
MLGMFERVPKFVKRYRDMAGVVSGAVQDYADEVRSRSFPTEDQIYAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
STLPKTLTLDTSTSRANPTPQPMKRLTVPRIRQRKGGEPIVMLTAYTVRMAQLLDPHCD
CCCCCEEEEECCCCCCCCCHHHHHHCCCHHHHHHCCCCCEEEEHHHHHHHHHHHCCCCC
MLLVGDSLAQVIYGLPHTVGVTMEMMALHGAAVVRGSYHAAVIVDMPFGSYEGSPQQAFD
EEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCCCCCCHHHHHH
NAARLLKETGAAAVKVEGGKVLAPTIEFLTQRGIPVMGHVGLTPQAVNILGGYGVRGKSE
HHHHHHHHCCCEEEEECCCEEEHHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCCCCCCH
EEARSIVEDAVAVAQAGAFSIVIEGVLESIAIEITNKVACPTIGIGASAQCDGQVLVTDD
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCEEEEEHH
MLGMFERVPKFVKRYRDMAGVVSGAVQDYADEVRSRSFPTEDQIYAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA