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The map label for this gene is ldh [H]

Identifier: 103487457

GI number: 103487457

Start: 2080695

End: 2081747

Strand: Reverse

Name: ldh [H]

Synonym: Sala_1973

Alternate gene names: 103487457

Gene position: 2081747-2080695 (Counterclockwise)

Preceding gene: 103487459

Following gene: 103487456

Centisome position: 62.23

GC content: 70.28

Gene sequence:

>1053_bases
ATGGTCGTCCGTCTTGCCCGCCTGGCCCCGCCACTCGAATGCGTGCGCCTCTATGATCTCGAAGCCGGGCTCGACGGGTT
TATCGCGATTCACTCGACCGCGCTGGGGCCGGGCGCCGGCGGCTGTCGCCTCTGGTCCTACCCCGACGCGAGCCATGCGC
TCGCCGACGCGGTGCGGCTGGCCGAGGGGATGAGTTACAAGAACGCGCTTGCCGGCTTGCCGCTGGGCGGTGCGAAGGCG
GTGCTGCGGCGGCCCGAGGGCGAATGGGATCGCGTCGCGCTGTTCCGCGCCTTTGGCCGCGCGGTCGAGGAGCTGGGCGG
GCTTTATGTCACTGCTGAGGATGTCGGGACGAGCGTTGCGGATATGCAGGAGGTCGCGCAGACGTCGCGCCATGTCGCGG
GCTTGCCGTCGGCCGAGGGACGCGCGGGAGGCGATCCGTCGCCCTGGACCGCCCAGGGGGTATTCGATGCGATGACGGTC
GCCGCCGAGTTCGCGCTGGGCAAAAGCCTTGATGGCCTGACTGTCGCGGTGCAGGGCACGGGCAATGTCGGCGCCGATCT
GTGCCGCCGCCTCGCCGATGCGGGTGCGCGGCTGGTGATCGCCGATGTCAGCCCGGTGCGCCGCGACCGGCTGAAGGCCG
TGCTCGGCGCGCAGGTCGTCGACGTATCCGAAATCGCTTCGGTCGAAGCTGACATCTTCGCGCCCTGTGCGCTCGGCGGC
GCGCTCGACCGCGACACGGCGGCGAGGCTGAAGGCGAAGATCGTGTGCGGCGCGGCGAACAACCAGCTGGCGAGCCCCGA
CGTCGCCGCGCAGCTGCGCGATCGCGGGATCGTCTATGCGCCCGACTATGTTGTCAACGCGGGCGGCATCATCAACGTTT
CGGCCGAATATCTGGGCGAGGACGTCCGCGACGTCGAATTGCGCGTCGCCGAAATCGGCCCGCGCGTCGGCGCGCTGCTC
GAACGCGCGGCGCGCGAGGGACGCTCCCCTGCATTCGTCGCCGACGAAATGGCCGAAGAGGTGATCGCGGGCGCCAAGCG
CGTGGCGGCCTGA

Upstream 100 bases:

>100_bases
CGAACATAATATCCCGAAAATCCGGCGAACAATAAAGATGCGATCAAGAACGCCCTGCCGAACGATGCGATAAGCGACGC
ATCGTTCAAGGAGAATGTTC

Downstream 100 bases:

>100_bases
GGGCCCGCGCCGTGTACCGCTTTGAAATCGACGCGACGCCCGACCCGCAGGCGCTGCCGCGCGTTGCCAATGTTTTCGCG
CAGCGCGCGCTCGTGCCCGC

Product: Glu/Leu/Phe/Val dehydrogenase, dimerisation region

Products: NA

Alternate protein names: LeuDH [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MVVRLARLAPPLECVRLYDLEAGLDGFIAIHSTALGPGAGGCRLWSYPDASHALADAVRLAEGMSYKNALAGLPLGGAKA
VLRRPEGEWDRVALFRAFGRAVEELGGLYVTAEDVGTSVADMQEVAQTSRHVAGLPSAEGRAGGDPSPWTAQGVFDAMTV
AAEFALGKSLDGLTVAVQGTGNVGADLCRRLADAGARLVIADVSPVRRDRLKAVLGAQVVDVSEIASVEADIFAPCALGG
ALDRDTAARLKAKIVCGAANNQLASPDVAAQLRDRGIVYAPDYVVNAGGIINVSAEYLGEDVRDVELRVAEIGPRVGALL
ERAAREGRSPAFVADEMAEEVIAGAKRVAA

Sequences:

>Translated_350_residues
MVVRLARLAPPLECVRLYDLEAGLDGFIAIHSTALGPGAGGCRLWSYPDASHALADAVRLAEGMSYKNALAGLPLGGAKA
VLRRPEGEWDRVALFRAFGRAVEELGGLYVTAEDVGTSVADMQEVAQTSRHVAGLPSAEGRAGGDPSPWTAQGVFDAMTV
AAEFALGKSLDGLTVAVQGTGNVGADLCRRLADAGARLVIADVSPVRRDRLKAVLGAQVVDVSEIASVEADIFAPCALGG
ALDRDTAARLKAKIVCGAANNQLASPDVAAQLRDRGIVYAPDYVVNAGGIINVSAEYLGEDVRDVELRVAEIGPRVGALL
ERAAREGRSPAFVADEMAEEVIAGAKRVAA
>Mature_350_residues
MVVRLARLAPPLECVRLYDLEAGLDGFIAIHSTALGPGAGGCRLWSYPDASHALADAVRLAEGMSYKNALAGLPLGGAKA
VLRRPEGEWDRVALFRAFGRAVEELGGLYVTAEDVGTSVADMQEVAQTSRHVAGLPSAEGRAGGDPSPWTAQGVFDAMTV
AAEFALGKSLDGLTVAVQGTGNVGADLCRRLADAGARLVIADVSPVRRDRLKAVLGAQVVDVSEIASVEADIFAPCALGG
ALDRDTAARLKAKIVCGAANNQLASPDVAAQLRDRGIVYAPDYVVNAGGIINVSAEYLGEDVRDVELRVAEIGPRVGALL
ERAAREGRSPAFVADEMAEEVIAGAKRVAA

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids. Catalyzes the reversible deamination of these branched-chain L-amino acids to their oxo analogs [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=319, Percent_Identity=28.5266457680251, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4885281, Length=319, Percent_Identity=28.5266457680251, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24649283, Length=318, Percent_Identity=27.6729559748428, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.9 [H]

Molecular weight: Translated: 36306; Mature: 36306

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVRLARLAPPLECVRLYDLEAGLDGFIAIHSTALGPGAGGCRLWSYPDASHALADAVRL
CEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHH
AEGMSYKNALAGLPLGGAKAVLRRPEGEWDRVALFRAFGRAVEELGGLYVTAEDVGTSVA
HHCCCHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEHHHHCCHHH
DMQEVAQTSRHVAGLPSAEGRAGGDPSPWTAQGVFDAMTVAAEFALGKSLDGLTVAVQGT
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
GNVGADLCRRLADAGARLVIADVSPVRRDRLKAVLGAQVVDVSEIASVEADIFAPCALGG
CCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
ALDRDTAARLKAKIVCGAANNQLASPDVAAQLRDRGIVYAPDYVVNAGGIINVSAEYLGE
CCCCHHHHHHEEEEEEECCCCCCCCCHHHHHHHHCCCEECCCEEECCCCEEEEEHHHCCC
DVRDVELRVAEIGPRVGALLERAAREGRSPAFVADEMAEEVIAGAKRVAA
CHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVVRLARLAPPLECVRLYDLEAGLDGFIAIHSTALGPGAGGCRLWSYPDASHALADAVRL
CEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHH
AEGMSYKNALAGLPLGGAKAVLRRPEGEWDRVALFRAFGRAVEELGGLYVTAEDVGTSVA
HHCCCHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEHHHHCCHHH
DMQEVAQTSRHVAGLPSAEGRAGGDPSPWTAQGVFDAMTVAAEFALGKSLDGLTVAVQGT
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
GNVGADLCRRLADAGARLVIADVSPVRRDRLKAVLGAQVVDVSEIASVEADIFAPCALGG
CCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
ALDRDTAARLKAKIVCGAANNQLASPDVAAQLRDRGIVYAPDYVVNAGGIINVSAEYLGE
CCCCHHHHHHEEEEEEECCCCCCCCCHHHHHHHHCCCEECCCEEECCCCEEEEEHHHCCC
DVRDVELRVAEIGPRVGALLERAAREGRSPAFVADEMAEEVIAGAKRVAA
CHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8020469 [H]