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The map label for this gene is ykfA [H]

Identifier: 103487382

GI number: 103487382

Start: 1997835

End: 1998662

Strand: Reverse

Name: ykfA [H]

Synonym: Sala_1898

Alternate gene names: 103487382

Gene position: 1998662-1997835 (Counterclockwise)

Preceding gene: 103487387

Following gene: 103487381

Centisome position: 59.75

GC content: 67.15

Gene sequence:

>828_bases
ATGCGTATTGGCATCGTTGCCCCCTCGACCCCGATCCTGCCCGACGATGCCGAGGCGGTGCGCGCGCTGGCCAGTCTCGG
CTACCCCGGCGTCGAACTGATCTTCGACCCGCAATGTTTCGCAATGCATGGCCATTTCGCGGGTGAGGACGGGCACCGTT
TTGCCGCGCTCGTCGAAATGGCGAACCGTCCCGACATCGATGCGCTGTGGTTCGCGCGCGGCGGCTATGGTGCGTGCCGG
ATCGCCGAGGAGGCGGTCGCGGCGATGGCCGATGTAGCCCGCGCCAAAGCCTTCCTCGGCTATTCGGATCAGGGCAATCT
GCTGGCGGCGCTCTATCGCGACGGCTTTGACCATGTCGCGCACGGACCGATGGTCGCCGATATCCGGCGCGAAGGCGGCG
AGGCGGCGGTGACGCGCGCGCTCGACTGGCTGGTCGCGCGCGATCCCGCGGCGTGCGAGCCGGGGCTGGAGCATGGCGCG
CGCCATGCGGCGTTCAACCTGATGACGCTGTCGATGCTGCTCGGCACCCCGCTCGAACCCGATCTGGCTGGGCATGTGCT
GCTGATCGAGGAGGTCAGCGAATATCTATACGCCTTTGACCGGGCGCTGTTTCATGTGACGAGCTATCTGGCGCCGCGCG
GGCTTGCCGGACTCCGGCTGGGGCGGGTTAGCGACATTCCGGAAAACGACCGGCCTTTTGGACTGGACGCGGAAGAAATT
GCGCAGGGCTGGTGCTCGCGCACCGGCATCCCCTGGCTCGGTCGCGCCGACATCGGCCATGATGCGGCGAACAAGGTCGT
GCCCTTCGGCTTGCATCGGGCGGGGTGA

Upstream 100 bases:

>100_bases
GGGTGGCGTGGAAATCGGACGGCGGACACCCTCATCCAACTTCGCCTAGCCAGCAAGTCGGCAAGGCTGCGTATCCTTCT
CCCATGGGGAGAAGGTTTTA

Downstream 100 bases:

>100_bases
AGGAATAGGCGCGAGGCCTTTCCGTCGCCCCCGCGAAGGCGGGGGCCGTTGTCGGCCCTGCGCCGATGGCGCGGAACAAA
ACTCCAGCGGCCCCCGCCTT

Product: peptidase U61, LD-carboxypeptidase A

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MRIGIVAPSTPILPDDAEAVRALASLGYPGVELIFDPQCFAMHGHFAGEDGHRFAALVEMANRPDIDALWFARGGYGACR
IAEEAVAAMADVARAKAFLGYSDQGNLLAALYRDGFDHVAHGPMVADIRREGGEAAVTRALDWLVARDPAACEPGLEHGA
RHAAFNLMTLSMLLGTPLEPDLAGHVLLIEEVSEYLYAFDRALFHVTSYLAPRGLAGLRLGRVSDIPENDRPFGLDAEEI
AQGWCSRTGIPWLGRADIGHDAANKVVPFGLHRAG

Sequences:

>Translated_275_residues
MRIGIVAPSTPILPDDAEAVRALASLGYPGVELIFDPQCFAMHGHFAGEDGHRFAALVEMANRPDIDALWFARGGYGACR
IAEEAVAAMADVARAKAFLGYSDQGNLLAALYRDGFDHVAHGPMVADIRREGGEAAVTRALDWLVARDPAACEPGLEHGA
RHAAFNLMTLSMLLGTPLEPDLAGHVLLIEEVSEYLYAFDRALFHVTSYLAPRGLAGLRLGRVSDIPENDRPFGLDAEEI
AQGWCSRTGIPWLGRADIGHDAANKVVPFGLHRAG
>Mature_275_residues
MRIGIVAPSTPILPDDAEAVRALASLGYPGVELIFDPQCFAMHGHFAGEDGHRFAALVEMANRPDIDALWFARGGYGACR
IAEEAVAAMADVARAKAFLGYSDQGNLLAALYRDGFDHVAHGPMVADIRREGGEAAVTRALDWLVARDPAACEPGLEHGA
RHAAFNLMTLSMLLGTPLEPDLAGHVLLIEEVSEYLYAFDRALFHVTSYLAPRGLAGLRLGRVSDIPENDRPFGLDAEEI
AQGWCSRTGIPWLGRADIGHDAANKVVPFGLHRAG

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=244, Percent_Identity=27.4590163934426, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 29548; Mature: 29548

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIVAPSTPILPDDAEAVRALASLGYPGVELIFDPQCFAMHGHFAGEDGHRFAALVEM
CEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHH
ANRPDIDALWFARGGYGACRIAEEAVAAMADVARAKAFLGYSDQGNLLAALYRDGFDHVA
HCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCHHHHH
HGPMVADIRREGGEAAVTRALDWLVARDPAACEPGLEHGARHAAFNLMTLSMLLGTPLEP
CCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHCHHHHHHHHHHHHHHHCCCCCC
DLAGHVLLIEEVSEYLYAFDRALFHVTSYLAPRGLAGLRLGRVSDIPENDRPFGLDAEEI
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCHHHH
AQGWCSRTGIPWLGRADIGHDAANKVVPFGLHRAG
HHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRIGIVAPSTPILPDDAEAVRALASLGYPGVELIFDPQCFAMHGHFAGEDGHRFAALVEM
CEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHH
ANRPDIDALWFARGGYGACRIAEEAVAAMADVARAKAFLGYSDQGNLLAALYRDGFDHVA
HCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCHHHHH
HGPMVADIRREGGEAAVTRALDWLVARDPAACEPGLEHGARHAAFNLMTLSMLLGTPLEP
CCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHCHHHHHHHHHHHHHHHCCCCCC
DLAGHVLLIEEVSEYLYAFDRALFHVTSYLAPRGLAGLRLGRVSDIPENDRPFGLDAEEI
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCHHHH
AQGWCSRTGIPWLGRADIGHDAANKVVPFGLHRAG
HHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]