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The map label for this gene is gcvA [H]

Identifier: 103487092

GI number: 103487092

Start: 1685734

End: 1686612

Strand: Direct

Name: gcvA [H]

Synonym: Sala_1607

Alternate gene names: 103487092

Gene position: 1685734-1686612 (Clockwise)

Preceding gene: 103487090

Following gene: 103487098

Centisome position: 50.39

GC content: 66.55

Gene sequence:

>879_bases
ATGCCCCGCCTTCCTCCCCTCAGCAGCATGGAAGCCTTTCTCGAGGTCGCCCGCCACGGCACGGTCAAGGCGGCCGCCAT
CGAGCTCGGCCTGTCGATGCCCGCGCTGTCGCGCCGAATCCAGACGCTCGAACATGCGGTCGGACGCCCGCTTTTCAACC
GCCACCATCATGGACTCAGCCTGACCGAGACGGGGCGGACACTGCAAGCCCAGCTCTCTCCCATCCTCGACGAGCTGCGC
GCGGTCATCGCGCAGGCGGGCAGCCCCGACGCCTCGCTGCGCCTGCACCTCAACGTCCTGCCGCTCTTCGCCCAACAGCG
GCTGTTCCCGCGCCTGCCCGAACTGCGACGCCAGCATCCCGAACTGCATATCGACATCGACACCATGTCGCATGCCGAGG
CGCGGCTGGGCGACGGCATCGACGCGGCGATCGCGCTCGCGCGCTCAATCGATCCGGCGCTCTATGCGGCGCGGCTGGAC
CAGGACAAGGTATTCCCGATCGCGGCGCGCACGCTGACCGACGGCGACCGGCCGATCACCGTGCCCGAACAATTGCAGCG
GATGACCGTGCTTTTGCACCGCGAAATGCCCGAAACCTTCACCGAATGGAAAAAGGCGATCGGCATGACCTATCTCGAAC
CCGCGGGGATCGACTATTTCGATTCGGGGCCGCTGATGCTCGAGGCGGCGGCGCAGGGCATCGGCATCGCCTTCATGCAC
GGCCACCATTTCGACGACGCGCACGACCCGCGGCTCGTCCGCCTGTTCGACTATGACGTGGACAGTCCCTACAGCTACTG
GTTCGTCTGCCGCCCGCGCGCGCTTCGCCAGCCGGCGGTCAAATTGTTCCACGACTGGCTGCTCGCAGCGAGAATCTGA

Upstream 100 bases:

>100_bases
GACGGTTCCCGATTTGCGACCCCCGACTCCGGGCAATTCATGCCGCAGCGCACAAATTTGTGGCATGTCCGCGGCGCTCA
TGTCATCACCACCGGTCATC

Downstream 100 bases:

>100_bases
CATTCCAGCGGGGAAAATGGTGCTGCTGGACAGGATTGAACTGTCGACCTCGTCATTACCAATGACGCGCTCTACCACTG
AGCTACAGCAGCAACCGGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MPRLPPLSSMEAFLEVARHGTVKAAAIELGLSMPALSRRIQTLEHAVGRPLFNRHHHGLSLTETGRTLQAQLSPILDELR
AVIAQAGSPDASLRLHLNVLPLFAQQRLFPRLPELRRQHPELHIDIDTMSHAEARLGDGIDAAIALARSIDPALYAARLD
QDKVFPIAARTLTDGDRPITVPEQLQRMTVLLHREMPETFTEWKKAIGMTYLEPAGIDYFDSGPLMLEAAAQGIGIAFMH
GHHFDDAHDPRLVRLFDYDVDSPYSYWFVCRPRALRQPAVKLFHDWLLAARI

Sequences:

>Translated_292_residues
MPRLPPLSSMEAFLEVARHGTVKAAAIELGLSMPALSRRIQTLEHAVGRPLFNRHHHGLSLTETGRTLQAQLSPILDELR
AVIAQAGSPDASLRLHLNVLPLFAQQRLFPRLPELRRQHPELHIDIDTMSHAEARLGDGIDAAIALARSIDPALYAARLD
QDKVFPIAARTLTDGDRPITVPEQLQRMTVLLHREMPETFTEWKKAIGMTYLEPAGIDYFDSGPLMLEAAAQGIGIAFMH
GHHFDDAHDPRLVRLFDYDVDSPYSYWFVCRPRALRQPAVKLFHDWLLAARI
>Mature_291_residues
PRLPPLSSMEAFLEVARHGTVKAAAIELGLSMPALSRRIQTLEHAVGRPLFNRHHHGLSLTETGRTLQAQLSPILDELRA
VIAQAGSPDASLRLHLNVLPLFAQQRLFPRLPELRRQHPELHIDIDTMSHAEARLGDGIDAAIALARSIDPALYAARLDQ
DKVFPIAARTLTDGDRPITVPEQLQRMTVLLHREMPETFTEWKKAIGMTYLEPAGIDYFDSGPLMLEAAAQGIGIAFMHG
HHFDDAHDPRLVRLFDYDVDSPYSYWFVCRPRALRQPAVKLFHDWLLAARI

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=288, Percent_Identity=28.125, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788706, Length=238, Percent_Identity=28.1512605042017, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1787128, Length=145, Percent_Identity=31.0344827586207, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789440, Length=240, Percent_Identity=25.4166666666667, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787601, Length=119, Percent_Identity=33.6134453781513, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786448, Length=238, Percent_Identity=28.1512605042017, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI157672245, Length=180, Percent_Identity=31.1111111111111, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32822; Mature: 32691

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLPPLSSMEAFLEVARHGTVKAAAIELGLSMPALSRRIQTLEHAVGRPLFNRHHHGLS
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCE
LTETGRTLQAQLSPILDELRAVIAQAGSPDASLRLHLNVLPLFAQQRLFPRLPELRRQHP
ECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHHHCCCCHHHHHHCC
ELHIDIDTMSHAEARLGDGIDAAIALARSIDPALYAARLDQDKVFPIAARTLTDGDRPIT
CEEEEECCCCHHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCCCEEEEEHHEECCCCCCCC
VPEQLQRMTVLLHREMPETFTEWKKAIGMTYLEPAGIDYFDSGPLMLEAAAQGIGIAFMH
HHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEECCCCCCEECCCCEEEEEHHCCCEEEEEE
GHHFDDAHDPRLVRLFDYDVDSPYSYWFVCRPRALRQPAVKLFHDWLLAARI
CCCCCCCCCCEEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PRLPPLSSMEAFLEVARHGTVKAAAIELGLSMPALSRRIQTLEHAVGRPLFNRHHHGLS
CCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCE
LTETGRTLQAQLSPILDELRAVIAQAGSPDASLRLHLNVLPLFAQQRLFPRLPELRRQHP
ECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHHHCCCCHHHHHHCC
ELHIDIDTMSHAEARLGDGIDAAIALARSIDPALYAARLDQDKVFPIAARTLTDGDRPIT
CEEEEECCCCHHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCCCEEEEEHHEECCCCCCCC
VPEQLQRMTVLLHREMPETFTEWKKAIGMTYLEPAGIDYFDSGPLMLEAAAQGIGIAFMH
HHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEECCCCCCEECCCCEEEEEHHCCCEEEEEE
GHHFDDAHDPRLVRLFDYDVDSPYSYWFVCRPRALRQPAVKLFHDWLLAARI
CCCCCCCCCCEEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]