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The map label for this gene is nitA [H]

Identifier: 103486874

GI number: 103486874

Start: 1443591

End: 1444607

Strand: Reverse

Name: nitA [H]

Synonym: Sala_1388

Alternate gene names: 103486874

Gene position: 1444607-1443591 (Counterclockwise)

Preceding gene: 103486875

Following gene: 103486873

Centisome position: 43.18

GC content: 67.45

Gene sequence:

>1017_bases
ATGAGCGATACGTCGGCCTCCCTCCTGCCCAAATCGCGCGTCGCCGCAGTGCAGGCGGCGCCGGTGTTCCTCGATCCGGC
AGCGACGGTCGACAAGGCGTGTGCGTTGATCGCCGAGGCGGCCGGAAATGGCGCGCAGCTCGTCGCCTTCCCGGAGGTGT
TCGTCCCCGCCTATCCCTATTGGAACTGGCTGATGACCCCGATCGAGGGCGCCGAATGGCACGAGCGGCTGTATCGCGCG
TCGGTCCGCGTGGACGGCCCGGAGGTCGCCGCACTCTGCGCCGCGGCGCGCGACCATGGCTGCACGCTCGTCATCGGCAT
CAACGAGCGCGATCCGGTGAGCGTCGGCACGCTCTATAACAGCAACCTCGTCATCGGCGCCGACGGCAGCCTGCTCGGGC
GTCATCGCAAGCTGGTGCCGACCTGGGCCGAAAAGCTGACATGGGCGGGCGGCGACGGCAGCTCGATCCGCGTCCATGAC
ACGTCGGTCGGGCCGCTCGGCACGCTGGCGTGCGGGGAGAATACGAACACGCTCGCGCGCTTCGCCTTGCTGTCGCAGGG
CGAACTGGTCCACGTCGCCAATTATATCGCGCTGCCTGTCGCTCCCGCCTCCTACAATATGGCCGAAGCGATCAGGATCC
GCGCCACCGCGCACAGCTTCGAGGGCAAGGTCTTCACCATCGTCGCCTGTTCGGCGCTGAGCGAGGAGATCATCGCGGCG
ATGTCCGAGGGTCGCCCGCAGAACCGCGACCTGCTGTCGCGCCCGAACAGCGCCTGGTCGGGCGTGATCGACCCGCATGG
CCATCCGGTCGGCGAGCCGCTGATCGACGCCGAAGGCATCGTCTATGCCGACATCGACCTCGGCGAGTGTATCCGGCCCA
AGCTGATGCACGACATCATCGGCGGCTATAACCGCTTCGACATCTTCAACCTGACGGTCGACCGGACGCCCCGCGCGTCG
GCGCGGTTTGTCGACGCCGAGCAGCCTGTCGATCCTGACGAGGAGACACCGCCATGA

Upstream 100 bases:

>100_bases
TGCGGTACAGCGACATCGTCGAGACGGTCGAACGGCTCGAAAGGGTCTGCGCCACCCGCGCGTGGGACAAGCCGCAATAT
CACCAGCGGGCGGCGGTGAC

Downstream 100 bases:

>100_bases
CAGGCCCTTCGACGATCATCGATCCGCGCGACGACGTGCTCGGCCGGTCGCGCGTCACCGACACGCCCGAGCTGGAGGCG
TTTTACGAAGAGCTCGCGGC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSDTSASLLPKSRVAAVQAAPVFLDPAATVDKACALIAEAAGNGAQLVAFPEVFVPAYPYWNWLMTPIEGAEWHERLYRA
SVRVDGPEVAALCAAARDHGCTLVIGINERDPVSVGTLYNSNLVIGADGSLLGRHRKLVPTWAEKLTWAGGDGSSIRVHD
TSVGPLGTLACGENTNTLARFALLSQGELVHVANYIALPVAPASYNMAEAIRIRATAHSFEGKVFTIVACSALSEEIIAA
MSEGRPQNRDLLSRPNSAWSGVIDPHGHPVGEPLIDAEGIVYADIDLGECIRPKLMHDIIGGYNRFDIFNLTVDRTPRAS
ARFVDAEQPVDPDEETPP

Sequences:

>Translated_338_residues
MSDTSASLLPKSRVAAVQAAPVFLDPAATVDKACALIAEAAGNGAQLVAFPEVFVPAYPYWNWLMTPIEGAEWHERLYRA
SVRVDGPEVAALCAAARDHGCTLVIGINERDPVSVGTLYNSNLVIGADGSLLGRHRKLVPTWAEKLTWAGGDGSSIRVHD
TSVGPLGTLACGENTNTLARFALLSQGELVHVANYIALPVAPASYNMAEAIRIRATAHSFEGKVFTIVACSALSEEIIAA
MSEGRPQNRDLLSRPNSAWSGVIDPHGHPVGEPLIDAEGIVYADIDLGECIRPKLMHDIIGGYNRFDIFNLTVDRTPRAS
ARFVDAEQPVDPDEETPP
>Mature_337_residues
SDTSASLLPKSRVAAVQAAPVFLDPAATVDKACALIAEAAGNGAQLVAFPEVFVPAYPYWNWLMTPIEGAEWHERLYRAS
VRVDGPEVAALCAAARDHGCTLVIGINERDPVSVGTLYNSNLVIGADGSLLGRHRKLVPTWAEKLTWAGGDGSSIRVHDT
SVGPLGTLACGENTNTLARFALLSQGELVHVANYIALPVAPASYNMAEAIRIRATAHSFEGKVFTIVACSALSEEIIAAM
SEGRPQNRDLLSRPNSAWSGVIDPHGHPVGEPLIDAEGIVYADIDLGECIRPKLMHDIIGGYNRFDIFNLTVDRTPRASA
RFVDAEQPVDPDEETPP

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17557111, Length=316, Percent_Identity=32.5949367088608, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6322027, Length=190, Percent_Identity=31.5789473684211, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 36155; Mature: 36024

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTSASLLPKSRVAAVQAAPVFLDPAATVDKACALIAEAAGNGAQLVAFPEVFVPAYPY
CCCCCCHHCCHHHHHHEEECCEEECCHHHHHHHHHHHHHHCCCCEEEEEECHHHCCCCCC
WNWLMTPIEGAEWHERLYRASVRVDGPEVAALCAAARDHGCTLVIGINERDPVSVGTLYN
CHHEECCCCCHHHHHHHHHHHEEECCHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEC
SNLVIGADGSLLGRHRKLVPTWAEKLTWAGGDGSSIRVHDTSVGPLGTLACGENTNTLAR
CCEEEECCCCHHCCCCCCCCCHHHHEEECCCCCCEEEEEECCCCCCEEEEECCCCCHHHH
FALLSQGELVHVANYIALPVAPASYNMAEAIRIRATAHSFEGKVFTIVACSALSEEIIAA
HHHHCCCCEEEEEEEEEEECCCCCCCHHHEEEEEEEECCCCCCEEEEEEEHHHHHHHHHH
MSEGRPQNRDLLSRPNSAWSGVIDPHGHPVGEPLIDAEGIVYADIDLGECIRPKLMHDII
HHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
GGYNRFDIFNLTVDRTPRASARFVDAEQPVDPDEETPP
CCCCEEEEEEEEECCCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SDTSASLLPKSRVAAVQAAPVFLDPAATVDKACALIAEAAGNGAQLVAFPEVFVPAYPY
CCCCCHHCCHHHHHHEEECCEEECCHHHHHHHHHHHHHHCCCCEEEEEECHHHCCCCCC
WNWLMTPIEGAEWHERLYRASVRVDGPEVAALCAAARDHGCTLVIGINERDPVSVGTLYN
CHHEECCCCCHHHHHHHHHHHEEECCHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEC
SNLVIGADGSLLGRHRKLVPTWAEKLTWAGGDGSSIRVHDTSVGPLGTLACGENTNTLAR
CCEEEECCCCHHCCCCCCCCCHHHHEEECCCCCCEEEEEECCCCCCEEEEECCCCCHHHH
FALLSQGELVHVANYIALPVAPASYNMAEAIRIRATAHSFEGKVFTIVACSALSEEIIAA
HHHHCCCCEEEEEEEEEEECCCCCCCHHHEEEEEEEECCCCCCEEEEEEEHHHHHHHHHH
MSEGRPQNRDLLSRPNSAWSGVIDPHGHPVGEPLIDAEGIVYADIDLGECIRPKLMHDII
HHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
GGYNRFDIFNLTVDRTPRASARFVDAEQPVDPDEETPP
CCCCEEEEEEEEECCCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]