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The map label for this gene is thyA [H]

Identifier: 103486818

GI number: 103486818

Start: 1395082

End: 1396044

Strand: Reverse

Name: thyA [H]

Synonym: Sala_1332

Alternate gene names: 103486818

Gene position: 1396044-1395082 (Counterclockwise)

Preceding gene: 103486820

Following gene: 103486817

Centisome position: 41.73

GC content: 61.47

Gene sequence:

>963_bases
ATGACAGGGAAAGAAGGGGAGCACGTCTTGGCTGATTCCATCCACTATGAACTGCAATATCTCGACCTGATGCGCCGTAT
CTGGGTCGACGGTGACGAGCGCGTCGACCGCACCGGCGTCGGCACGCGCTCGCTGTTCGGTGAGACGATGCGATTCTCGC
TGAAGGACGACGCCATCCCCCTGCTCACGACGAAGCGCGTTTATTGGAAGACTGCACTGCGCGAACTGCTTTGGTTTCTC
ACGGGCGACACCAATATCCGTCCGCTCGTTGCGCAAGGCGTAAAGATCTGGACCGACTGGCCGCTCGAGAAATATCGCAA
GGCGACCGGCGAGGCGATCAGCGCCACGGATTTCGAAGCGCGGATCGTCGCAGACGCGGATTTTGCGGCCAAATGGGGCG
ACCTTGGTCCCGTCTACGGTCATCAATGGGTCAACTGGCCGCGCTACGAGCCGGCGGGCGGCGGTCTGTTTCGCCGCGCC
GACCACGGGTATAACCAGATTGCGGCGCTGATCGACTCGCTCAAGAACAATCCGGGGTCACGGCGCCACATCTTCACCGG
CTGGAATGTCGCCGATCTCGACCGGATGGCGTTGCCGCCGTGCCATATGACCTATCAGTTCCATGTTCGCAGCGACGGCG
GATTGTCGTGCCTGCTCTTTCAGCGCTCGTGCGATCTTGGTCTTGGCTTCGCGTTCAACATTTTCGAGGCGGCGTTGCTC
ACGCGGATGGTCGCCGAGCAATGCGGCCTTTACGCGCATGAGGTCGTGTGGACCGGTGGCGACGTCCATCTCTATCTCAA
CCACGCCGAGCTGGTCGAACAGCAGTTGGCGCGCGTACCGGAGGGAAAGCCCCGGCTCCGCATCCTGCGCCGACCGCCCA
GCATTTTCGAATATCGGTTCGAGGATTTCGTGGTCGAGGATTACGCGCCGCAAGCGCATATTTCCGCGCCGGTGGCGGTC
TGA

Upstream 100 bases:

>100_bases
AGGTGCGTCGCGGACCCAACAGCCGACTGCCCCGCATTGGATGGATCGGCGGCGGCGGACGGGGCGACGCCCTTGCCGCC
GTGCAGTGCGGAGCCTAGCA

Downstream 100 bases:

>100_bases
CAGCTGCCGTTGCATTCGCATGGCGGCCGTAATAATATGTCGCCACGATGAAATATAACTAGGCGCCGATTCGCGCCCGA
AGGGAAGAAGATATGCCTGA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTGKEGEHVLADSIHYELQYLDLMRRIWVDGDERVDRTGVGTRSLFGETMRFSLKDDAIPLLTTKRVYWKTALRELLWFL
TGDTNIRPLVAQGVKIWTDWPLEKYRKATGEAISATDFEARIVADADFAAKWGDLGPVYGHQWVNWPRYEPAGGGLFRRA
DHGYNQIAALIDSLKNNPGSRRHIFTGWNVADLDRMALPPCHMTYQFHVRSDGGLSCLLFQRSCDLGLGFAFNIFEAALL
TRMVAEQCGLYAHEVVWTGGDVHLYLNHAELVEQQLARVPEGKPRLRILRRPPSIFEYRFEDFVVEDYAPQAHISAPVAV

Sequences:

>Translated_320_residues
MTGKEGEHVLADSIHYELQYLDLMRRIWVDGDERVDRTGVGTRSLFGETMRFSLKDDAIPLLTTKRVYWKTALRELLWFL
TGDTNIRPLVAQGVKIWTDWPLEKYRKATGEAISATDFEARIVADADFAAKWGDLGPVYGHQWVNWPRYEPAGGGLFRRA
DHGYNQIAALIDSLKNNPGSRRHIFTGWNVADLDRMALPPCHMTYQFHVRSDGGLSCLLFQRSCDLGLGFAFNIFEAALL
TRMVAEQCGLYAHEVVWTGGDVHLYLNHAELVEQQLARVPEGKPRLRILRRPPSIFEYRFEDFVVEDYAPQAHISAPVAV
>Mature_319_residues
TGKEGEHVLADSIHYELQYLDLMRRIWVDGDERVDRTGVGTRSLFGETMRFSLKDDAIPLLTTKRVYWKTALRELLWFLT
GDTNIRPLVAQGVKIWTDWPLEKYRKATGEAISATDFEARIVADADFAAKWGDLGPVYGHQWVNWPRYEPAGGGLFRRAD
HGYNQIAALIDSLKNNPGSRRHIFTGWNVADLDRMALPPCHMTYQFHVRSDGGLSCLLFQRSCDLGLGFAFNIFEAALLT
RMVAEQCGLYAHEVVWTGGDVHLYLNHAELVEQQLARVPEGKPRLRILRRPPSIFEYRFEDFVVEDYAPQAHISAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=311, Percent_Identity=43.4083601286174, Blast_Score=232, Evalue=3e-61,
Organism=Escherichia coli, GI1789191, Length=302, Percent_Identity=47.682119205298, Blast_Score=268, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI71993377, Length=305, Percent_Identity=39.672131147541, Blast_Score=214, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6324648, Length=317, Percent_Identity=35.0157728706625, Blast_Score=170, Evalue=2e-43,
Organism=Drosophila melanogaster, GI17137556, Length=310, Percent_Identity=42.9032258064516, Blast_Score=242, Evalue=3e-64,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 36466; Mature: 36335

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKEGEHVLADSIHYELQYLDLMRRIWVDGDERVDRTGVGTRSLFGETMRFSLKDDAIP
CCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHEEEECCCCCC
LLTTKRVYWKTALRELLWFLTGDTNIRPLVAQGVKIWTDWPLEKYRKATGEAISATDFEA
EEECHHHHHHHHHHHHHHHHCCCCCCCHHHHCCEEEEECCCHHHHHHHCCCEEECCCCEE
RIVADADFAAKWGDLGPVYGHQWVNWPRYEPAGGGLFRRADHGYNQIAALIDSLKNNPGS
EEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
RRHIFTGWNVADLDRMALPPCHMTYQFHVRSDGGLSCLLFQRSCDLGLGFAFNIFEAALL
CCEEEECCCHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHH
TRMVAEQCGLYAHEVVWTGGDVHLYLNHAELVEQQLARVPEGKPRLRILRRPPSIFEYRF
HHHHHHHHCCEEEEEEEECCEEEEEECHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHH
EDFVVEDYAPQAHISAPVAV
HHHEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGKEGEHVLADSIHYELQYLDLMRRIWVDGDERVDRTGVGTRSLFGETMRFSLKDDAIP
CCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHEEEECCCCCC
LLTTKRVYWKTALRELLWFLTGDTNIRPLVAQGVKIWTDWPLEKYRKATGEAISATDFEA
EEECHHHHHHHHHHHHHHHHCCCCCCCHHHHCCEEEEECCCHHHHHHHCCCEEECCCCEE
RIVADADFAAKWGDLGPVYGHQWVNWPRYEPAGGGLFRRADHGYNQIAALIDSLKNNPGS
EEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
RRHIFTGWNVADLDRMALPPCHMTYQFHVRSDGGLSCLLFQRSCDLGLGFAFNIFEAALL
CCEEEECCCHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHH
TRMVAEQCGLYAHEVVWTGGDVHLYLNHAELVEQQLARVPEGKPRLRILRRPPSIFEYRF
HHHHHHHHCCEEEEEEEECCEEEEEECHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHH
EDFVVEDYAPQAHISAPVAV
HHHEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA