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The map label for this gene is bkdA1 [H]

Identifier: 103486817

GI number: 103486817

Start: 1393679

End: 1394989

Strand: Reverse

Name: bkdA1 [H]

Synonym: Sala_1331

Alternate gene names: 103486817

Gene position: 1394989-1393679 (Counterclockwise)

Preceding gene: 103486818

Following gene: 103486816

Centisome position: 41.7

GC content: 63.54

Gene sequence:

>1311_bases
ATGCCTGAAACGCCTGCCCGGGCGGATAAGGGGCGTCCGGCGAGCAACCTGCCGCCGCTGTCGCTTCATATCCCCGAACC
GCGCTATCGCCCCGGCGACACGCCCGACTTCAGCGACATCGAAATCCCTGCGGTTGAGGCGACCCCGCGTCCCGGTGAAG
CGACCAGGCCCGACGCAATGCGCGAGCTTTGCTATGGCCTTGTCCGCGTCCTCGATTTCGACGGCATCGCCAAGGGGCCA
TGGAATCCCAACCTCGCCCCCGACCGGCTGCGCACCATGCTGCGTTACATGATGCTGACGCGCGCGTTCGACGATCGCAT
GTTCCGCGCACAGCGGCAGGGCAAGACCAGCTTCTACATGAAATGCACGGGCGAGGAGGCAACCTCGATCGCCTCGACGA
TGGCGATCGACCGCGCCGACATGTGCTTCCCCAGCTATCGCCAGCAGGGCATTTTGATCGCGCGCGACTATCCGCTGATC
CAGATGATGAACCAGATTTACTCGAATCGCGGCGATCACCTGTTGGGGCGGCAACTCCCGATCATGTATTCGGCGCCCGA
ACATGGGTTTTTCAGCGTGTCGGGCAACCTCGCCACCCAATATCCACAGGCGGTGGGCTGGGCGATGGCATCGGCGTCGA
AGGGCGACACGCGGATCGCGACCGTGTGGTGCGGCGAAGGCTCGTCCGCCGAGGGGGATTTCCACTCAGCGCTGACTTTC
GCCACCGTTTATAATGCCCCCGTGATTTTCAATGTCGTCAATAACCAGTGGGCTATTTCGAGCTTTTCGGGCTTTGCCGG
CGGCGAGCGCACGACCTTTGCGGCGCGTGCGGTGGGTTATGGCATCGCGGGGCTGCGCGTCGATGGCAACGACCCGCTCG
CGGTCTATGCGGCAACGCAATGGGCAGCCGATCGCGCGCGGACGAACAACGGCCCGACGCTGATCGAACATTTCACCTAT
CGCGCCGAAGGGCACAGCACCTCGGACGACCCCGGCGCCTATCGCGCGGCGCAGGAAGCGAGCGCCTGGCCGTTCGGCGA
CCCGATCGCGCGGCTCAAGCAGCATCTGATCGCGCTTGGCGAATGGGACGACGCGCGCCACGAAGCGCAGGCGAAGGAAC
TCGACGATCTGGTCAAGACGACGCAGAAGCAGTCGGAAAAGCTTGGCATATTGGGTCACGGGATGCACCAGCCGTTCGAA
ACGATGTTCGAGGACGTTTTCGAGGAGATGCCCTGGCACCTCAAGGAACAATGCGCGCAGATGCTCGCCGAGCAAGAGGC
CAAGTTCGGCCCCGACTGGAAGCCCGAATGA

Upstream 100 bases:

>100_bases
CGGTCTGACAGCTGCCGTTGCATTCGCATGGCGGCCGTAATAATATGTCGCCACGATGAAATATAACTAGGCGCCGATTC
GCGCCCGAAGGGAAGAAGAT

Downstream 100 bases:

>100_bases
TGAGCGCCGAAACCCGGACGAAAACCATGAACATGATCGAGGCGATCAACAGCGCCATGGACGTCATGCTCGAACGCGAT
CCCGCGACCGTCGTGATGGG

Product: 3-methyl-2-oxobutanoate dehydrogenase (lipoamide)

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MPETPARADKGRPASNLPPLSLHIPEPRYRPGDTPDFSDIEIPAVEATPRPGEATRPDAMRELCYGLVRVLDFDGIAKGP
WNPNLAPDRLRTMLRYMMLTRAFDDRMFRAQRQGKTSFYMKCTGEEATSIASTMAIDRADMCFPSYRQQGILIARDYPLI
QMMNQIYSNRGDHLLGRQLPIMYSAPEHGFFSVSGNLATQYPQAVGWAMASASKGDTRIATVWCGEGSSAEGDFHSALTF
ATVYNAPVIFNVVNNQWAISSFSGFAGGERTTFAARAVGYGIAGLRVDGNDPLAVYAATQWAADRARTNNGPTLIEHFTY
RAEGHSTSDDPGAYRAAQEASAWPFGDPIARLKQHLIALGEWDDARHEAQAKELDDLVKTTQKQSEKLGILGHGMHQPFE
TMFEDVFEEMPWHLKEQCAQMLAEQEAKFGPDWKPE

Sequences:

>Translated_436_residues
MPETPARADKGRPASNLPPLSLHIPEPRYRPGDTPDFSDIEIPAVEATPRPGEATRPDAMRELCYGLVRVLDFDGIAKGP
WNPNLAPDRLRTMLRYMMLTRAFDDRMFRAQRQGKTSFYMKCTGEEATSIASTMAIDRADMCFPSYRQQGILIARDYPLI
QMMNQIYSNRGDHLLGRQLPIMYSAPEHGFFSVSGNLATQYPQAVGWAMASASKGDTRIATVWCGEGSSAEGDFHSALTF
ATVYNAPVIFNVVNNQWAISSFSGFAGGERTTFAARAVGYGIAGLRVDGNDPLAVYAATQWAADRARTNNGPTLIEHFTY
RAEGHSTSDDPGAYRAAQEASAWPFGDPIARLKQHLIALGEWDDARHEAQAKELDDLVKTTQKQSEKLGILGHGMHQPFE
TMFEDVFEEMPWHLKEQCAQMLAEQEAKFGPDWKPE
>Mature_435_residues
PETPARADKGRPASNLPPLSLHIPEPRYRPGDTPDFSDIEIPAVEATPRPGEATRPDAMRELCYGLVRVLDFDGIAKGPW
NPNLAPDRLRTMLRYMMLTRAFDDRMFRAQRQGKTSFYMKCTGEEATSIASTMAIDRADMCFPSYRQQGILIARDYPLIQ
MMNQIYSNRGDHLLGRQLPIMYSAPEHGFFSVSGNLATQYPQAVGWAMASASKGDTRIATVWCGEGSSAEGDFHSALTFA
TVYNAPVIFNVVNNQWAISSFSGFAGGERTTFAARAVGYGIAGLRVDGNDPLAVYAATQWAADRARTNNGPTLIEHFTYR
AEGHSTSDDPGAYRAAQEASAWPFGDPIARLKQHLIALGEWDDARHEAQAKELDDLVKTTQKQSEKLGILGHGMHQPFET
MFEDVFEEMPWHLKEQCAQMLAEQEAKFGPDWKPE

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=371, Percent_Identity=38.8140161725067, Blast_Score=244, Evalue=9e-65,
Organism=Homo sapiens, GI258645172, Length=369, Percent_Identity=38.7533875338753, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI4885543, Length=303, Percent_Identity=28.7128712871287, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI291084742, Length=242, Percent_Identity=29.7520661157025, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI4505685, Length=242, Percent_Identity=29.7520661157025, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI291084744, Length=235, Percent_Identity=29.3617021276596, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI291084757, Length=129, Percent_Identity=37.2093023255814, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI86563355, Length=339, Percent_Identity=38.3480825958702, Blast_Score=221, Evalue=6e-58,
Organism=Caenorhabditis elegans, GI86563357, Length=339, Percent_Identity=38.3480825958702, Blast_Score=221, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI17536047, Length=293, Percent_Identity=24.9146757679181, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32564172, Length=293, Percent_Identity=24.9146757679181, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321026, Length=322, Percent_Identity=28.2608695652174, Blast_Score=120, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21355903, Length=367, Percent_Identity=36.2397820163488, Blast_Score=218, Evalue=7e-57,
Organism=Drosophila melanogaster, GI24639748, Length=329, Percent_Identity=28.2674772036474, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24639744, Length=239, Percent_Identity=28.8702928870293, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28571106, Length=239, Percent_Identity=28.8702928870293, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24639740, Length=239, Percent_Identity=28.8702928870293, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24639746, Length=239, Percent_Identity=28.8702928870293, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR022593 [H]

Pfam domain/function: PF00676 E1_dh; PF12573 OxoDH_E1alpha_N [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 48450; Mature: 48319

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETPARADKGRPASNLPPLSLHIPEPRYRPGDTPDFSDIEIPAVEATPRPGEATRPDAM
CCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHH
RELCYGLVRVLDFDGIAKGPWNPNLAPDRLRTMLRYMMLTRAFDDRMFRAQRQGKTSFYM
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
KCTGEEATSIASTMAIDRADMCFPSYRQQGILIARDYPLIQMMNQIYSNRGDHLLGRQLP
EECCCHHHHHHHHHHHCHHHHCCHHHHCCCEEEECCCHHHHHHHHHHHCCCCEECCCCCC
IMYSAPEHGFFSVSGNLATQYPQAVGWAMASASKGDTRIATVWCGEGSSAEGDFHSALTF
EEEECCCCCEEEECCCCHHHCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHH
ATVYNAPVIFNVVNNQWAISSFSGFAGGERTTFAARAVGYGIAGLRVDGNDPLAVYAATQ
HHHHCCCEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCEEEEEEHH
WAADRARTNNGPTLIEHFTYRAEGHSTSDDPGAYRAAQEASAWPFGDPIARLKQHLIALG
HHHHHHCCCCCCEEEHHEEEECCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHC
EWDDARHEAQAKELDDLVKTTQKQSEKLGILGHGMHQPFETMFEDVFEEMPWHLKEQCAQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
MLAEQEAKFGPDWKPE
HHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PETPARADKGRPASNLPPLSLHIPEPRYRPGDTPDFSDIEIPAVEATPRPGEATRPDAM
CCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHH
RELCYGLVRVLDFDGIAKGPWNPNLAPDRLRTMLRYMMLTRAFDDRMFRAQRQGKTSFYM
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
KCTGEEATSIASTMAIDRADMCFPSYRQQGILIARDYPLIQMMNQIYSNRGDHLLGRQLP
EECCCHHHHHHHHHHHCHHHHCCHHHHCCCEEEECCCHHHHHHHHHHHCCCCEECCCCCC
IMYSAPEHGFFSVSGNLATQYPQAVGWAMASASKGDTRIATVWCGEGSSAEGDFHSALTF
EEEECCCCCEEEECCCCHHHCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHH
ATVYNAPVIFNVVNNQWAISSFSGFAGGERTTFAARAVGYGIAGLRVDGNDPLAVYAATQ
HHHHCCCEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCEEEEEEHH
WAADRARTNNGPTLIEHFTYRAEGHSTSDDPGAYRAAQEASAWPFGDPIARLKQHLIALG
HHHHHHCCCCCCEEEHHEEEECCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHC
EWDDARHEAQAKELDDLVKTTQKQSEKLGILGHGMHQPFETMFEDVFEEMPWHLKEQCAQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
MLAEQEAKFGPDWKPE
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]