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The map label for this gene is bkdA2 [H]

Identifier: 103486816

GI number: 103486816

Start: 1392648

End: 1393679

Strand: Reverse

Name: bkdA2 [H]

Synonym: Sala_1330

Alternate gene names: 103486816

Gene position: 1393679-1392648 (Counterclockwise)

Preceding gene: 103486817

Following gene: 103486815

Centisome position: 41.66

GC content: 62.69

Gene sequence:

>1032_bases
ATGAGCGCCGAAACCCGGACGAAAACCATGAACATGATCGAGGCGATCAACAGCGCCATGGACGTCATGCTCGAACGCGA
TCCCGCGACCGTCGTGATGGGCGAGGACGTCGGCTATTTCGGCGGCGTTTTCCGCGCCACCGCGGGCCTCCAGAAAAAGC
ATGGCAAGACGCGCGTGTTCGACACGCCGATCAACGAATGCGGCATCATCGGCGTCGCGGTCGGCATGGGCGCCTATGGT
CTGCGCCCCGTCCCCGAAATCCAGTTCGCCGATTATATCTATCCGGGGCTCGACCAGCTCGTCAGCGAGGCGGCGCGCCT
GCGCTATCGCTCGGCGAACGACTATATATGCCCGATGACGGTGCGCACACCGTTCGGCGGCGGGATTTTCGGCGGCCAGA
CGCACAGCCAGTCGCCCGAAAGCATCATGACGCATATCTGCGGCGTCAAGACGGTGATCCCGTCGAACCCCTATGATGCC
AAGGGGCTGCTGATCGCGGCGATCGAGGATAACGACCCCGTCGTCTTCCTCGAACCCAAGCGCATCTATAACGGCCCGTT
CAGCGGCTATTACGATCGCCCGGTCGAACCCTGGTCGAAGCATGACGCCAGTGCGGTGCCCGAGGGCTATTACCGCATCG
ACCTGGGGAAAGCGGCGACGGTGCGCGAGGGCGAAGCGGTGACCGTACTCGCCTATGGCACAATGGTTCATGTCGCAAAG
ACGATCATCGAGGAAATGGGGATCGACGCCGAAATCCTCGACCTGCGCACGCTGTTGCCGCTCGACATAGCGGCGATCGA
GGCGTCGGTGAAAAAGACCGGCCGCTGCCTGATCATCCACGAAGCGACGCGCACGTCGGGTTTTGGCGCCGAACTCGCCG
CGCTGGTGCAGGAACGCTGCTTCTATCATCTCGAGGCGCCCGTCGAGCGCGTCACCGGTTTCGACACGCCCTATCCGCAC
AGCCTGGAATGGGCCTATTTCCCCGGCCCGGTGCGCATTGCGACCGCGCTGACCAAGATTTTGAAGGACTGA

Upstream 100 bases:

>100_bases
AGGACGTTTTCGAGGAGATGCCCTGGCACCTCAAGGAACAATGCGCGCAGATGCTCGCCGAGCAAGAGGCCAAGTTCGGC
CCCGACTGGAAGCCCGAATG

Downstream 100 bases:

>100_bases
CGCCATGGCCCGCTATTCATTCCGTCTGCCCGACATTGGCGAAGGTATCGCCGAGGCCGAGATCGTTGCCTGGCACGTCA
AGGTGGGCGAGCGCGTCGAG

Product: transketolase, central region

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSAETRTKTMNMIEAINSAMDVMLERDPATVVMGEDVGYFGGVFRATAGLQKKHGKTRVFDTPINECGIIGVAVGMGAYG
LRPVPEIQFADYIYPGLDQLVSEAARLRYRSANDYICPMTVRTPFGGGIFGGQTHSQSPESIMTHICGVKTVIPSNPYDA
KGLLIAAIEDNDPVVFLEPKRIYNGPFSGYYDRPVEPWSKHDASAVPEGYYRIDLGKAATVREGEAVTVLAYGTMVHVAK
TIIEEMGIDAEILDLRTLLPLDIAAIEASVKKTGRCLIIHEATRTSGFGAELAALVQERCFYHLEAPVERVTGFDTPYPH
SLEWAYFPGPVRIATALTKILKD

Sequences:

>Translated_343_residues
MSAETRTKTMNMIEAINSAMDVMLERDPATVVMGEDVGYFGGVFRATAGLQKKHGKTRVFDTPINECGIIGVAVGMGAYG
LRPVPEIQFADYIYPGLDQLVSEAARLRYRSANDYICPMTVRTPFGGGIFGGQTHSQSPESIMTHICGVKTVIPSNPYDA
KGLLIAAIEDNDPVVFLEPKRIYNGPFSGYYDRPVEPWSKHDASAVPEGYYRIDLGKAATVREGEAVTVLAYGTMVHVAK
TIIEEMGIDAEILDLRTLLPLDIAAIEASVKKTGRCLIIHEATRTSGFGAELAALVQERCFYHLEAPVERVTGFDTPYPH
SLEWAYFPGPVRIATALTKILKD
>Mature_342_residues
SAETRTKTMNMIEAINSAMDVMLERDPATVVMGEDVGYFGGVFRATAGLQKKHGKTRVFDTPINECGIIGVAVGMGAYGL
RPVPEIQFADYIYPGLDQLVSEAARLRYRSANDYICPMTVRTPFGGGIFGGQTHSQSPESIMTHICGVKTVIPSNPYDAK
GLLIAAIEDNDPVVFLEPKRIYNGPFSGYYDRPVEPWSKHDASAVPEGYYRIDLGKAATVREGEAVTVLAYGTMVHVAKT
IIEEMGIDAEILDLRTLLPLDIAAIEASVKKTGRCLIIHEATRTSGFGAELAALVQERCFYHLEAPVERVTGFDTPYPHS
LEWAYFPGPVRIATALTKILKD

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=342, Percent_Identity=45.0292397660819, Blast_Score=310, Evalue=1e-84,
Organism=Homo sapiens, GI34101272, Length=342, Percent_Identity=45.0292397660819, Blast_Score=310, Evalue=1e-84,
Organism=Homo sapiens, GI156564403, Length=335, Percent_Identity=36.4179104477612, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI291084858, Length=335, Percent_Identity=35.5223880597015, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17506935, Length=335, Percent_Identity=46.5671641791045, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17538422, Length=340, Percent_Identity=37.6470588235294, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319698, Length=344, Percent_Identity=35.4651162790698, Blast_Score=196, Evalue=6e-51,
Organism=Drosophila melanogaster, GI160714828, Length=340, Percent_Identity=45, Blast_Score=296, Evalue=1e-80,
Organism=Drosophila melanogaster, GI160714832, Length=340, Percent_Identity=45, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI21358145, Length=340, Percent_Identity=35.5882352941176, Blast_Score=201, Evalue=8e-52,
Organism=Drosophila melanogaster, GI24650940, Length=340, Percent_Identity=35.5882352941176, Blast_Score=201, Evalue=8e-52,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=41.7582417582418, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=41.7582417582418, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI160714826, Length=67, Percent_Identity=50.7462686567164, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 37512; Mature: 37381

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAETRTKTMNMIEAINSAMDVMLERDPATVVMGEDVGYFGGVFRATAGLQKKHGKTRVF
CCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEE
DTPINECGIIGVAVGMGAYGLRPVPEIQFADYIYPGLDQLVSEAARLRYRSANDYICPMT
ECCCCCCCEEEHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEE
VRTPFGGGIFGGQTHSQSPESIMTHICGVKTVIPSNPYDAKGLLIAAIEDNDPVVFLEPK
EECCCCCCCCCCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCEEEEEEECCCCEEEECCH
RIYNGPFSGYYDRPVEPWSKHDASAVPEGYYRIDLGKAATVREGEAVTVLAYGTMVHVAK
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEECCCCEEEEEEHHHHHHHHH
TIIEEMGIDAEILDLRTLLPLDIAAIEASVKKTGRCLIIHEATRTSGFGAELAALVQERC
HHHHHHCCCCEEHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHH
FYHLEAPVERVTGFDTPYPHSLEWAYFPGPVRIATALTKILKD
HHHHCCCHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAETRTKTMNMIEAINSAMDVMLERDPATVVMGEDVGYFGGVFRATAGLQKKHGKTRVF
CCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEE
DTPINECGIIGVAVGMGAYGLRPVPEIQFADYIYPGLDQLVSEAARLRYRSANDYICPMT
ECCCCCCCEEEHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEE
VRTPFGGGIFGGQTHSQSPESIMTHICGVKTVIPSNPYDAKGLLIAAIEDNDPVVFLEPK
EECCCCCCCCCCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCEEEEEEECCCCEEEECCH
RIYNGPFSGYYDRPVEPWSKHDASAVPEGYYRIDLGKAATVREGEAVTVLAYGTMVHVAK
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEECCCCEEEEEEHHHHHHHHH
TIIEEMGIDAEILDLRTLLPLDIAAIEASVKKTGRCLIIHEATRTSGFGAELAALVQERC
HHHHHHCCCCEEHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHH
FYHLEAPVERVTGFDTPYPHSLEWAYFPGPVRIATALTKILKD
HHHHCCCHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]