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The map label for this gene is nuoN

Identifier: 103486782

GI number: 103486782

Start: 1354888

End: 1356318

Strand: Reverse

Name: nuoN

Synonym: Sala_1296

Alternate gene names: 103486782

Gene position: 1356318-1354888 (Counterclockwise)

Preceding gene: 103486783

Following gene: 103486781

Centisome position: 40.55

GC content: 64.08

Gene sequence:

>1431_bases
ATGACCGCCGATCTCGCGCTCATCTGGCCCGAACTGATCCTGACGATCGGCGGGCTGATCACCCTGATGCTCGGCACCTT
CATGGGCGACCGGCAGGTGGGCACCTACCAGCTCAGCGCGCTGCTCACGCTTGCCGCCGCAGCGGCCGCCGCTATCGCGC
TGTTCGGCGTCGAGACGACGGTGTTTTCAGGCACGCTGTCGGTCGACGCCTTCGGTGGCTTCGCCAAGCTGCTGATCTAC
GCCGCGAGCTTTATCTGCATTCTCGTCGCGCCGCGCTTCTTCACCGGCGGGATGCGCGCCGAATATCCGACGCTGATCCT
GTTCGCGGCGCTCGGCATGGGCATCATGGCCTCGTCGCGCGACCTGATGACGCTTTACGTCGGGCTCGAACTCAACAGCC
TCGCCGCCTATGTGCTCGCGAGCTTCATGCGGTCCGACGAACGGTCGAGCGAAGCGGGGCTCAAATATTTCGTGCTTGGC
GCGCTCGCATCGGGGATGCTCCTCTACGGCATCTCGCTGCTCTATGGCTTCACCGGCACCACCGATTTCGCAGGCATTGC
GGCGGCGATGGGCGGCGAGCCGGGCATCGGCCTGATCTTCGGCATCGTCTTCGTGCTTTCGGGGCTGGGCTTCAAGATCA
GCGCCGTGCCGTTCCATATGTGGACCCCCGACGTCTATGAAGGCGCGCCGACGCCGGTGACGACCTTCTTTGCCAGCGCG
CCCAAGGTGGCGGCGATGGCGCTGATGGCGCGCATCGTCATCGATGCGATGGGCCCCGCGGTCGGCGCCTGGCAACAGAT
CGTCATCTTCCTCGCGCTCGCGTCGATCATCCTCGGCGCGGTTGGCGCGATCGGGCAGAAGAACATCAAACGCCTGCTCG
CCTATTCGTCGATCAACAATGTCGGTTTCATGCTCATCGGCCTTGCCGCCGGAACGCAGCAGGGGGTTGAGGGCGTGCTG
ACCTATCTGCTCGTCTATCTCGTGACGACGCTCGGCGCCTTCCTCGTCGTCCTGCAACTGCGCGATGAGGGCGGCAATCA
GGTCGAAAGCATCCCCGCGCTCGCCGGCCTGTCGCAACGGCGACCGGGGCTGGCCGCCGCGATGTCGGTGTTCCTGTTCA
GCCTTGCCGGCATTCCGCCGCTGTTCGGCTTCTGGCCCAAATATCTGGTGTTCGAGGCTGCGGTGAACGCGAACCTTGTG
CCGCTGGCGGTTGCGGGCATCGTCGCATCGGTGATCGGCGCATTCTATTATATCGCGATCATCAAGACGATGTATTTCGA
TGATAAATCTGACGTCGAGGTGACAGGCGGCGGCAATGCCGTCGAGGGCACGATCGTCGCGGCGAGCGCGCTTTGGCTGT
TGGCGGTTGGTTATCTGTTCATCCCGGCGCTTGCGGTGGCTTCGGCGCACGCCGCATCGGTGCTGTTCTGA

Upstream 100 bases:

>100_bases
CGAAGCCCGCGGGCGACGCGCATCTGGCCGTCGGCAAGCCGAAGCCCGCCGCAGTGCATGAGGAAGCCGCCCACGGATCG
AGCCATGCGGGAGGCGTGCA

Downstream 100 bases:

>100_bases
TCCCGCCGATCGAGCGTATCGCGCAGACAGGTTCGACCAATGCCGACCTGCTGGCGCGGCTGGCGGGCGGCGAGCATGTC
GGCGAGGGTCACTGGCTGGT

Product: NADH dehydrogenase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTADLALIWPELILTIGGLITLMLGTFMGDRQVGTYQLSALLTLAAAAAAAIALFGVETTVFSGTLSVDAFGGFAKLLIY
AASFICILVAPRFFTGGMRAEYPTLILFAALGMGIMASSRDLMTLYVGLELNSLAAYVLASFMRSDERSSEAGLKYFVLG
ALASGMLLYGISLLYGFTGTTDFAGIAAAMGGEPGIGLIFGIVFVLSGLGFKISAVPFHMWTPDVYEGAPTPVTTFFASA
PKVAAMALMARIVIDAMGPAVGAWQQIVIFLALASIILGAVGAIGQKNIKRLLAYSSINNVGFMLIGLAAGTQQGVEGVL
TYLLVYLVTTLGAFLVVLQLRDEGGNQVESIPALAGLSQRRPGLAAAMSVFLFSLAGIPPLFGFWPKYLVFEAAVNANLV
PLAVAGIVASVIGAFYYIAIIKTMYFDDKSDVEVTGGGNAVEGTIVAASALWLLAVGYLFIPALAVASAHAASVLF

Sequences:

>Translated_476_residues
MTADLALIWPELILTIGGLITLMLGTFMGDRQVGTYQLSALLTLAAAAAAAIALFGVETTVFSGTLSVDAFGGFAKLLIY
AASFICILVAPRFFTGGMRAEYPTLILFAALGMGIMASSRDLMTLYVGLELNSLAAYVLASFMRSDERSSEAGLKYFVLG
ALASGMLLYGISLLYGFTGTTDFAGIAAAMGGEPGIGLIFGIVFVLSGLGFKISAVPFHMWTPDVYEGAPTPVTTFFASA
PKVAAMALMARIVIDAMGPAVGAWQQIVIFLALASIILGAVGAIGQKNIKRLLAYSSINNVGFMLIGLAAGTQQGVEGVL
TYLLVYLVTTLGAFLVVLQLRDEGGNQVESIPALAGLSQRRPGLAAAMSVFLFSLAGIPPLFGFWPKYLVFEAAVNANLV
PLAVAGIVASVIGAFYYIAIIKTMYFDDKSDVEVTGGGNAVEGTIVAASALWLLAVGYLFIPALAVASAHAASVLF
>Mature_475_residues
TADLALIWPELILTIGGLITLMLGTFMGDRQVGTYQLSALLTLAAAAAAAIALFGVETTVFSGTLSVDAFGGFAKLLIYA
ASFICILVAPRFFTGGMRAEYPTLILFAALGMGIMASSRDLMTLYVGLELNSLAAYVLASFMRSDERSSEAGLKYFVLGA
LASGMLLYGISLLYGFTGTTDFAGIAAAMGGEPGIGLIFGIVFVLSGLGFKISAVPFHMWTPDVYEGAPTPVTTFFASAP
KVAAMALMARIVIDAMGPAVGAWQQIVIFLALASIILGAVGAIGQKNIKRLLAYSSINNVGFMLIGLAAGTQQGVEGVLT
YLLVYLVTTLGAFLVVLQLRDEGGNQVESIPALAGLSQRRPGLAAAMSVFLFSLAGIPPLFGFWPKYLVFEAAVNANLVP
LAVAGIVASVIGAFYYIAIIKTMYFDDKSDVEVTGGGNAVEGTIVAASALWLLAVGYLFIPALAVASAHAASVLF

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Homo sapiens, GI251831108, Length=304, Percent_Identity=25, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI145693160, Length=409, Percent_Identity=37.8973105134474, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1788829, Length=306, Percent_Identity=26.797385620915, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788827, Length=305, Percent_Identity=26.2295081967213, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788831, Length=305, Percent_Identity=25.2459016393443, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788614, Length=366, Percent_Identity=25.6830601092896, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788613, Length=224, Percent_Identity=26.7857142857143, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI2367154, Length=268, Percent_Identity=26.4925373134328, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUON_SPHAL (Q1GTL2)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616343.1
- GeneID:   4082588
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_1296
- NMPDR:   fig|317655.9.peg.1232
- HOGENOM:   HBG747830
- OMA:   MDMNTLL
- ProtClustDB:   PRK05777
- BioCyc:   SALA317655:SALA_1296-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: =1.6.99.5

Molecular weight: Translated: 49544; Mature: 49413

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10e77050)-; HASH(0x125e962c)-; HASH(0x125d2694)-; HASH(0x125ecce0)-; HASH(0x12130ce8)-; HASH(0xfe1be84)-; HASH(0x125d27f0)-; HASH(0x121fc1b0)-; HASH(0x125d2634)-; HASH(0x125e959c)-; HASH(0x11d02018)-; HASH(0x125d28ec)-; HASH(0x1221f57c)-; HASH(0x12200818)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADLALIWPELILTIGGLITLMLGTFMGDRQVGTYQLSALLTLAAAAAAAIALFGVETT
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VFSGTLSVDAFGGFAKLLIYAASFICILVAPRFFTGGMRAEYPTLILFAALGMGIMASSR
HEECEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
DLMTLYVGLELNSLAAYVLASFMRSDERSSEAGLKYFVLGALASGMLLYGISLLYGFTGT
CEEEEEECCCHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
TDFAGIAAAMGGEPGIGLIFGIVFVLSGLGFKISAVPFHMWTPDVYEGAPTPVTTFFASA
CHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCHHCCCCCCCHHHHHHCC
PKVAAMALMARIVIDAMGPAVGAWQQIVIFLALASIILGAVGAIGQKNIKRLLAYSSINN
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
VGFMLIGLAAGTQQGVEGVLTYLLVYLVTTLGAFLVVLQLRDEGGNQVESIPALAGLSQR
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
RPGLAAAMSVFLFSLAGIPPLFGFWPKYLVFEAAVNANLVPLAVAGIVASVIGAFYYIAI
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
IKTMYFDDKSDVEVTGGGNAVEGTIVAASALWLLAVGYLFIPALAVASAHAASVLF
HHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
TADLALIWPELILTIGGLITLMLGTFMGDRQVGTYQLSALLTLAAAAAAAIALFGVETT
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VFSGTLSVDAFGGFAKLLIYAASFICILVAPRFFTGGMRAEYPTLILFAALGMGIMASSR
HEECEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
DLMTLYVGLELNSLAAYVLASFMRSDERSSEAGLKYFVLGALASGMLLYGISLLYGFTGT
CEEEEEECCCHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
TDFAGIAAAMGGEPGIGLIFGIVFVLSGLGFKISAVPFHMWTPDVYEGAPTPVTTFFASA
CHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCHHCCCCCCCHHHHHHCC
PKVAAMALMARIVIDAMGPAVGAWQQIVIFLALASIILGAVGAIGQKNIKRLLAYSSINN
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
VGFMLIGLAAGTQQGVEGVLTYLLVYLVTTLGAFLVVLQLRDEGGNQVESIPALAGLSQR
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
RPGLAAAMSVFLFSLAGIPPLFGFWPKYLVFEAAVNANLVPLAVAGIVASVIGAFYYIAI
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
IKTMYFDDKSDVEVTGGGNAVEGTIVAASALWLLAVGYLFIPALAVASAHAASVLF
HHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA