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The map label for this gene is nuoM [H]

Identifier: 103486783

GI number: 103486783

Start: 1356315

End: 1357868

Strand: Reverse

Name: nuoM [H]

Synonym: Sala_1297

Alternate gene names: 103486783

Gene position: 1357868-1356315 (Counterclockwise)

Preceding gene: 103486784

Following gene: 103486782

Centisome position: 40.59

GC content: 62.61

Gene sequence:

>1554_bases
GTGAGCGGCTTTCCGATCCTTTCGCTGATGCTGGCGGTCCCGCTGATCGCCGCCATCCTCTGCCTCTATGCGGGCGACCG
TAACGCACGTGTTATCGCGCTCGCCGCGACGCTGATCGATTTTGCGCTGGGCATCGTGCTCTGGGCCAGTTTCGACATCG
GCGGCGCGCAGTGGCAGTTTACCGAGCGCGCTGACCTGTTCGGCCGCTTCCAGTGGGCACTCGGCATCGACGGCATGGCG
CTGATGCTGATCATGCTCAGCGTCTTCCTCATGCCGCTGTGCATCCTCGCGAGCTGGGAAGCGATCAAGAGCCGTGTCGG
GCTCTACATGGCGATGTTCCTGATCATGGAAGTCGTGATGATCGGCGTCTTCATGGCGCAGGATCTGCTGCTCTTTTACA
TCTTCTTCGAAGCCGGTCTGATCCCGATGTATTTCATCATCGGCATCTGGGGCGGCGCGAACCGCAAATATGCGGCGTTC
AAATTCTTTCTCTACACGCTGCTCGGTTCGGTGCTGATGCTGATCGCGATGATCGCGATGATCGCCGAGGCGGGCACGAC
CGACATCCCGACGCTGCTCAACTATGACTTCCCGGTCGAAATGCAGACATGGCTGTGGCTCGCCTTCTTCGCCAGCCTCG
CGGTCAAGATGCCGATGTGGCCCGTCCACACCTGGCTTCCCGACGCGCATGTGCAGGCGCCGACCGCGGGCTCGATGATC
CTGGCGGGTGTGCTCCTGAAGCTCGGCGCCTATGGCTTCCTGCGCTTCATGATGCCGATGTTCCCCGACGCTTCGGCGCA
GCTGATGTGGCTGGTGTTCGGTCTGTCGATGGTCGCGGTGGTCTATACCAGCCTTGTCGCGCTGGTGCAGAACGACATGA
AGAAGCTGATCGCCTATTCGTCGGTCGCCCACATGGCGATCGTCACCGCGGGCCTCTTCGCTTTCAACCAGCAGGGGATC
GAGGGCGCGCTGATCATCATGCTCAGCCATGGCGTCGTCTCGGCCGCGCTCTTCTTCTGCGTCGGCGTGATCTATGACCG
GCTGCATACGCGCGAGATCGATCGCTACGGCGGGCTTGCGGTCAACATGCCGGCCTATGCGCTCTTCTTCATGTTGTTCA
CCATGGCGTCGATCGGCTTGCCGGGGACGAGCGGTTTTGTCGGCGAGTTCCTGAGCCTGCTCGGTATCTACGAAGCGTCG
AGTGTCGTCGCCTTCGTCTGTACGACCGGGATCATCCTCGGCGCCGCGTACATGCTTTACCTCTATCGCCGCGTCGTGTT
CGGCGAACTGACCAAGGACGATGTGAAGGCGATGCCCGATCTCAACGTACGCGAATGGGCGATCATGGCGCCGCTCGCCG
CCGTGGCGCTGTGGATGGGCGTCTATCCCGAAAGTTTCCTCGCGCCGATGCGCGGCGACGTGACCACGGTGGTCGCACGC
CTCGCTCCCGCGAAGCCCGCGGGCGACGCGCATCTGGCCGTCGGCAAGCCGAAGCCCGCCGCAGTGCATGAGGAAGCCGC
CCACGGATCGAGCCATGCGGGAGGCGTGCAATGA

Upstream 100 bases:

>100_bases
GGCTCGCGGTCCGGCTGCAATCGGGTTATGTTTATGGATATGCGTTTGTGATGCTGCTCGGTCTTGTCGGCCTCGCCAGC
TGGGCCATGGTGAATTTCCT

Downstream 100 bases:

>100_bases
CCGCCGATCTCGCGCTCATCTGGCCCGAACTGATCCTGACGATCGGCGGGCTGATCACCCTGATGCTCGGCACCTTCATG
GGCGACCGGCAGGTGGGCAC

Product: proton-translocating NADH-quinone oxidoreductase, chain M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSGFPILSLMLAVPLIAAILCLYAGDRNARVIALAATLIDFALGIVLWASFDIGGAQWQFTERADLFGRFQWALGIDGMA
LMLIMLSVFLMPLCILASWEAIKSRVGLYMAMFLIMEVVMIGVFMAQDLLLFYIFFEAGLIPMYFIIGIWGGANRKYAAF
KFFLYTLLGSVLMLIAMIAMIAEAGTTDIPTLLNYDFPVEMQTWLWLAFFASLAVKMPMWPVHTWLPDAHVQAPTAGSMI
LAGVLLKLGAYGFLRFMMPMFPDASAQLMWLVFGLSMVAVVYTSLVALVQNDMKKLIAYSSVAHMAIVTAGLFAFNQQGI
EGALIIMLSHGVVSAALFFCVGVIYDRLHTREIDRYGGLAVNMPAYALFFMLFTMASIGLPGTSGFVGEFLSLLGIYEAS
SVVAFVCTTGIILGAAYMLYLYRRVVFGELTKDDVKAMPDLNVREWAIMAPLAAVALWMGVYPESFLAPMRGDVTTVVAR
LAPAKPAGDAHLAVGKPKPAAVHEEAAHGSSHAGGVQ

Sequences:

>Translated_517_residues
MSGFPILSLMLAVPLIAAILCLYAGDRNARVIALAATLIDFALGIVLWASFDIGGAQWQFTERADLFGRFQWALGIDGMA
LMLIMLSVFLMPLCILASWEAIKSRVGLYMAMFLIMEVVMIGVFMAQDLLLFYIFFEAGLIPMYFIIGIWGGANRKYAAF
KFFLYTLLGSVLMLIAMIAMIAEAGTTDIPTLLNYDFPVEMQTWLWLAFFASLAVKMPMWPVHTWLPDAHVQAPTAGSMI
LAGVLLKLGAYGFLRFMMPMFPDASAQLMWLVFGLSMVAVVYTSLVALVQNDMKKLIAYSSVAHMAIVTAGLFAFNQQGI
EGALIIMLSHGVVSAALFFCVGVIYDRLHTREIDRYGGLAVNMPAYALFFMLFTMASIGLPGTSGFVGEFLSLLGIYEAS
SVVAFVCTTGIILGAAYMLYLYRRVVFGELTKDDVKAMPDLNVREWAIMAPLAAVALWMGVYPESFLAPMRGDVTTVVAR
LAPAKPAGDAHLAVGKPKPAAVHEEAAHGSSHAGGVQ
>Mature_516_residues
SGFPILSLMLAVPLIAAILCLYAGDRNARVIALAATLIDFALGIVLWASFDIGGAQWQFTERADLFGRFQWALGIDGMAL
MLIMLSVFLMPLCILASWEAIKSRVGLYMAMFLIMEVVMIGVFMAQDLLLFYIFFEAGLIPMYFIIGIWGGANRKYAAFK
FFLYTLLGSVLMLIAMIAMIAEAGTTDIPTLLNYDFPVEMQTWLWLAFFASLAVKMPMWPVHTWLPDAHVQAPTAGSMIL
AGVLLKLGAYGFLRFMMPMFPDASAQLMWLVFGLSMVAVVYTSLVALVQNDMKKLIAYSSVAHMAIVTAGLFAFNQQGIE
GALIIMLSHGVVSAALFFCVGVIYDRLHTREIDRYGGLAVNMPAYALFFMLFTMASIGLPGTSGFVGEFLSLLGIYEASS
VVAFVCTTGIILGAAYMLYLYRRVVFGELTKDDVKAMPDLNVREWAIMAPLAAVALWMGVYPESFLAPMRGDVTTVVARL
APAKPAGDAHLAVGKPKPAAVHEEAAHGSSHAGGVQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=351, Percent_Identity=35.8974358974359, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI251831117, Length=444, Percent_Identity=22.2972972972973, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788613, Length=489, Percent_Identity=36.8098159509202, Blast_Score=287, Evalue=9e-79,
Organism=Escherichia coli, GI1788827, Length=312, Percent_Identity=27.2435897435897, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1788831, Length=303, Percent_Identity=28.0528052805281, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788614, Length=419, Percent_Identity=27.4463007159904, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI2367154, Length=386, Percent_Identity=25.1295336787565, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788829, Length=333, Percent_Identity=24.6246246246246, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 56314; Mature: 56183

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
6.8 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
6.6 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SGFPILSLMLAVPLIAAILCLYAGDRNARVIALAATLIDFALGIVLWASFDIGGAQWQF
CCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEE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HCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]