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The map label for this gene is dus [H]

Identifier: 103486763

GI number: 103486763

Start: 1334923

End: 1335921

Strand: Reverse

Name: dus [H]

Synonym: Sala_1277

Alternate gene names: 103486763

Gene position: 1335921-1334923 (Counterclockwise)

Preceding gene: 103486766

Following gene: 103486762

Centisome position: 39.94

GC content: 65.87

Gene sequence:

>999_bases
ATGGCGCCGCTTCGGCCCATCCAGATCGGCAACATCACCATCGCGGATCCCGTGATCCTCGCGCCGATGACGGGCGTCAC
CGACCTGCCGTTCCGCAAGCTCGTTCGCTATTATGGCTCCGGGCTCAACGTCACCGAGATGATCGCCAGCCAGGCAGCGA
TCCGCGAGACGCGCCAGTCGATCCAGAAAGCGGCGTGGGATCCGGTGGAGGAGCCCGTGTCGATGCAGCTCGTCGGCTGC
ACCCCCTATGAAATGGGCGAAGCGGCGAAGCTCAACGAGGATCGCGGCGCGGCGATCATCGACATCAACATGGGCTGCCC
GGTCCGCAAGGTCACCAATGGCGACGCCGGATCGGCGCTGATGCGCGACCTCAGGCTCGCTGCGGCGCTGATCGACGCCT
GTGTCAAGGCGGTGTCGGTCCCGGTAACGGTCAAGATGCGCATGGGCTGGTGCCATGACAGCCTCAATGCCCCCGAACTC
GCGCATATTGCCGAGGATCTGGGCGCAAAGCTCGTCACTGTCCACGGCCGCACGCGCAACCAGATGTATCGCGGCGAGGC
CGACTGGGGTTTTGTGCGCAAGGTCAAGGAGGCCGTGTCGATTCCCGTGATCGTCAACGGCGACATTTGCTCGGCCGACG
ACGCGCGCACCGCGCTGGCGCAAAGCGGCGCCGATGGCGTGATGATCGGCCGCGGCGCCTATGGCCGCCCCTGGCTGCTG
GGTCAGGTGATGGCGGCGCTGCGCGGCGAGGGCGAGCGGCCCGACCCCGGCCTCGACGAACAATATGACGTCATCACTTC
GCATTACCGCGCGATGATCGACCATTATGGCGAAACCACCGGGGTCAATATGGCGCGCAAGCACCTCGGCTGGTATGTCA
AGGGGCTGCCCGGATCGGCCGAGTTCCGCAACAAGGTCAACCGGATTCCCGACAGCAAGGGCGTGCTCGACGCGCTCGAG
GCCTTTTATGGCCCGCATCTGACCCGGGCCGCCGCGTGA

Upstream 100 bases:

>100_bases
TCATGGTGCGCGCCGGTTAGCGCAGCCACGCCTTGCGGGAAAGCGGCTTAGCGTGTAGGGGCTGCTCAAAATTTAGGCAG
TCTGACGAAAGCACCCCGCC

Downstream 100 bases:

>100_bases
CCACGCATGCGTCGAGCATCCCGAGCTTCGACCATGACGAGCTGATCCAGTCGCACCCGGTGCCGACGTTATTGATCGAC
AGCGGTGGGGTGGTCCTGTT

Product: dihydrouridine synthase TIM-barrel protein nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MAPLRPIQIGNITIADPVILAPMTGVTDLPFRKLVRYYGSGLNVTEMIASQAAIRETRQSIQKAAWDPVEEPVSMQLVGC
TPYEMGEAAKLNEDRGAAIIDINMGCPVRKVTNGDAGSALMRDLRLAAALIDACVKAVSVPVTVKMRMGWCHDSLNAPEL
AHIAEDLGAKLVTVHGRTRNQMYRGEADWGFVRKVKEAVSIPVIVNGDICSADDARTALAQSGADGVMIGRGAYGRPWLL
GQVMAALRGEGERPDPGLDEQYDVITSHYRAMIDHYGETTGVNMARKHLGWYVKGLPGSAEFRNKVNRIPDSKGVLDALE
AFYGPHLTRAAA

Sequences:

>Translated_332_residues
MAPLRPIQIGNITIADPVILAPMTGVTDLPFRKLVRYYGSGLNVTEMIASQAAIRETRQSIQKAAWDPVEEPVSMQLVGC
TPYEMGEAAKLNEDRGAAIIDINMGCPVRKVTNGDAGSALMRDLRLAAALIDACVKAVSVPVTVKMRMGWCHDSLNAPEL
AHIAEDLGAKLVTVHGRTRNQMYRGEADWGFVRKVKEAVSIPVIVNGDICSADDARTALAQSGADGVMIGRGAYGRPWLL
GQVMAALRGEGERPDPGLDEQYDVITSHYRAMIDHYGETTGVNMARKHLGWYVKGLPGSAEFRNKVNRIPDSKGVLDALE
AFYGPHLTRAAA
>Mature_331_residues
APLRPIQIGNITIADPVILAPMTGVTDLPFRKLVRYYGSGLNVTEMIASQAAIRETRQSIQKAAWDPVEEPVSMQLVGCT
PYEMGEAAKLNEDRGAAIIDINMGCPVRKVTNGDAGSALMRDLRLAAALIDACVKAVSVPVTVKMRMGWCHDSLNAPELA
HIAEDLGAKLVTVHGRTRNQMYRGEADWGFVRKVKEAVSIPVIVNGDICSADDARTALAQSGADGVMIGRGAYGRPWLLG
QVMAALRGEGERPDPGLDEQYDVITSHYRAMIDHYGETTGVNMARKHLGWYVKGLPGSAEFRNKVNRIPDSKGVLDALEA
FYGPHLTRAAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788462, Length=289, Percent_Identity=31.1418685121107, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI239788483, Length=283, Percent_Identity=31.095406360424, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI40807366, Length=262, Percent_Identity=31.2977099236641, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI31742496, Length=310, Percent_Identity=26.7741935483871, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI8923374, Length=300, Percent_Identity=26.6666666666667, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=41.875, Blast_Score=250, Evalue=8e-68,
Organism=Escherichia coli, GI1788462, Length=230, Percent_Identity=34.3478260869565, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI145693211, Length=310, Percent_Identity=29.0322580645161, Blast_Score=98, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17543114, Length=295, Percent_Identity=31.864406779661, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17507177, Length=226, Percent_Identity=30.0884955752212, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25144369, Length=223, Percent_Identity=30.9417040358744, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17510279, Length=264, Percent_Identity=28.7878787878788, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=229, Percent_Identity=33.1877729257642, Blast_Score=106, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6323433, Length=310, Percent_Identity=25.1612903225806, Blast_Score=89, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=248, Percent_Identity=28.2258064516129, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24585320, Length=285, Percent_Identity=31.9298245614035, Blast_Score=144, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24580595, Length=227, Percent_Identity=30.8370044052863, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19920448, Length=227, Percent_Identity=30.8370044052863, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19921524, Length=227, Percent_Identity=29.5154185022026, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45549423, Length=275, Percent_Identity=28.3636363636364, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35922; Mature: 35791

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPLRPIQIGNITIADPVILAPMTGVTDLPFRKLVRYYGSGLNVTEMIASQAAIRETRQS
CCCCCCEEECCEEECCCEEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
IQKAAWDPVEEPVSMQLVGCTPYEMGEAAKLNEDRGAAIIDINMGCPVRKVTNGDAGSAL
HHHHCCCCCCCCCCEEEEECCCHHCCCCCCCCCCCCCEEEEECCCCCEEECCCCCHHHHH
MRDLRLAAALIDACVKAVSVPVTVKMRMGWCHDSLNAPELAHIAEDLGAKLVTVHGRTRN
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCEEEEEECCCCC
QMYRGEADWGFVRKVKEAVSIPVIVNGDICSADDARTALAQSGADGVMIGRGAYGRPWLL
CCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCHHH
GQVMAALRGEGERPDPGLDEQYDVITSHYRAMIDHYGETTGVNMARKHLGWYVKGLPGSA
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCEEEECCCCCH
EFRNKVNRIPDSKGVLDALEAFYGPHLTRAAA
HHHHHHHCCCCCCHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
APLRPIQIGNITIADPVILAPMTGVTDLPFRKLVRYYGSGLNVTEMIASQAAIRETRQS
CCCCCEEECCEEECCCEEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
IQKAAWDPVEEPVSMQLVGCTPYEMGEAAKLNEDRGAAIIDINMGCPVRKVTNGDAGSAL
HHHHCCCCCCCCCCEEEEECCCHHCCCCCCCCCCCCCEEEEECCCCCEEECCCCCHHHHH
MRDLRLAAALIDACVKAVSVPVTVKMRMGWCHDSLNAPELAHIAEDLGAKLVTVHGRTRN
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCEEEEEECCCCC
QMYRGEADWGFVRKVKEAVSIPVIVNGDICSADDARTALAQSGADGVMIGRGAYGRPWLL
CCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCHHH
GQVMAALRGEGERPDPGLDEQYDVITSHYRAMIDHYGETTGVNMARKHLGWYVKGLPGSA
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCEEEECCCCCH
EFRNKVNRIPDSKGVLDALEAFYGPHLTRAAA
HHHHHHHCCCCCCHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]