Click here to switch to the map view.

The map label for this gene is ntrX [H]

Identifier: 103486759

GI number: 103486759

Start: 1328670

End: 1330055

Strand: Reverse

Name: ntrX [H]

Synonym: Sala_1273

Alternate gene names: 103486759

Gene position: 1330055-1328670 (Counterclockwise)

Preceding gene: 103486760

Following gene: 103486758

Centisome position: 39.76

GC content: 62.77

Gene sequence:

>1386_bases
ATGGCGCTTGATATTCTGATCGTCGATGATGAACGCGACATATGCGAGCTCGTCGCTGGGGTCATGGAAGACGAAGGCTA
TGAAGCGCGGACCGCGTCGGACAGCGATTCGGCGCTCGAAGCTATCCGGCAGCGGCGCCCGTCGCTGGCGCTGATCGATG
TCTGGCTCCAGGGCTCACGGCTCGACGGATTGGGACTTGTGGAGGCGATCAAGGCGTTCGACCCGACGCTGCCGATCATC
GTCATCTCGGGGCATGGCGGACTCGACACCGCGGTCGCCGCGATCCGCCGCGGCGCATTCGACTTCATCGAAAAGCCGTT
TGAAGCCTCGCGCCTGCTCCACCTCGTCGCGCGCGCGACCGAGAACGAACGGCTGAAGTTTGAATATGAACAACTCCGCG
AAAAGGCAGGTCCGGCGGAAGAACTCACGGGCAACAGCGCCGCGATCAACAATGTCCGCGCCACGCTGAAGCGCGTCGCA
GGGACGGGAAGCCGTGTTCTCATCACAGGCGCGCCCGGTGTCGGCAAGGAGGTGGCCGCCCGCGTTCTGCACGGCTGGAG
CGGGCGCCACAATGCGCCGTTCCGGGTGATCTCGTCGGCGCGGATGGATCCCGAGACGGTCGAGCTTGAGCTTTTCGGTT
CGGAGGCCGAGGATGGCTCGATCCGAGTCGGCCTTCTCGAACAGGCGCATGGCGGCACGCTGTTCCTTGATGAAGTCGCC
GACATGCCGCTGACAACGCAGGGCAAGATACTGCGGGTGTTGACCGACCAGAGTTTCACCCGCGTGGGAGGACGGACGAT
GATCCGCGTCGACGTCCGTATCATTTCCGGCTCGGCACGCGACCTGACCGTCGAGATCGCCGAAGGGCGCTTTCGCGAGG
ATCTATACTACCGGCTCAACGTCGTTCCGGTGCATATTCCGTCGCTGCGCGAAAGGCGCGACGATATCGCAAGCCTGTGC
GACCATTTCGTCCGTCGCTATGCGGCCGACCGCCGGGTTCCGCCGCCGGAGATCAGCGCCGAAGCGATGGCGGCGCTTCA
GGCGCACGACTGGCCGGGGAATGTGCGCGAACTGCGCAATGTGATCGAACGAGTGATGATTCTGGCGCCAAGCGACCGGC
TGGGCCGCATCGATGCGGACATGCTGCCCGCCGAACTGGTACGGGGAGGCGCCGATATTTTGCCCAGTTCCGAGTCCATC
ACCACCATTCCGCTCAAGGAAGCGCGCGAGAATTTCGAGCGCGAATATCTGCGCATCCAGATCAACCGTTTTTCGGGCAA
TATCTCGCGTACGGCGAGTTTCATCGGCATGGAGCGCTCGGCGCTGCACCGCAAACTGAAACTTTTGGGGCTCACCGAAA
GTTCGGATAATGAAAGTCCGGCTTGA

Upstream 100 bases:

>100_bases
GAACGGCTGCGGCCGCTGGCCGGGCAGGGCGACGACAATGGTCCGGGCAAGAGCGAGACGGTTCCGGGACGCATCCGCAA
CCGGAAGGACAGGGAAGACC

Downstream 100 bases:

>100_bases
CGCTGGACGGTAGGTGCGCTTTGCCGCATATTGCGAGCAAGAAGAAGGTCTTGAAAGCCGGAAAACCGGCGCCGTGTCCG
CGGTTTCACCGCCAAGAACA

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MALDILIVDDERDICELVAGVMEDEGYEARTASDSDSALEAIRQRRPSLALIDVWLQGSRLDGLGLVEAIKAFDPTLPII
VISGHGGLDTAVAAIRRGAFDFIEKPFEASRLLHLVARATENERLKFEYEQLREKAGPAEELTGNSAAINNVRATLKRVA
GTGSRVLITGAPGVGKEVAARVLHGWSGRHNAPFRVISSARMDPETVELELFGSEAEDGSIRVGLLEQAHGGTLFLDEVA
DMPLTTQGKILRVLTDQSFTRVGGRTMIRVDVRIISGSARDLTVEIAEGRFREDLYYRLNVVPVHIPSLRERRDDIASLC
DHFVRRYAADRRVPPPEISAEAMAALQAHDWPGNVRELRNVIERVMILAPSDRLGRIDADMLPAELVRGGADILPSSESI
TTIPLKEARENFEREYLRIQINRFSGNISRTASFIGMERSALHRKLKLLGLTESSDNESPA

Sequences:

>Translated_461_residues
MALDILIVDDERDICELVAGVMEDEGYEARTASDSDSALEAIRQRRPSLALIDVWLQGSRLDGLGLVEAIKAFDPTLPII
VISGHGGLDTAVAAIRRGAFDFIEKPFEASRLLHLVARATENERLKFEYEQLREKAGPAEELTGNSAAINNVRATLKRVA
GTGSRVLITGAPGVGKEVAARVLHGWSGRHNAPFRVISSARMDPETVELELFGSEAEDGSIRVGLLEQAHGGTLFLDEVA
DMPLTTQGKILRVLTDQSFTRVGGRTMIRVDVRIISGSARDLTVEIAEGRFREDLYYRLNVVPVHIPSLRERRDDIASLC
DHFVRRYAADRRVPPPEISAEAMAALQAHDWPGNVRELRNVIERVMILAPSDRLGRIDADMLPAELVRGGADILPSSESI
TTIPLKEARENFEREYLRIQINRFSGNISRTASFIGMERSALHRKLKLLGLTESSDNESPA
>Mature_460_residues
ALDILIVDDERDICELVAGVMEDEGYEARTASDSDSALEAIRQRRPSLALIDVWLQGSRLDGLGLVEAIKAFDPTLPIIV
ISGHGGLDTAVAAIRRGAFDFIEKPFEASRLLHLVARATENERLKFEYEQLREKAGPAEELTGNSAAINNVRATLKRVAG
TGSRVLITGAPGVGKEVAARVLHGWSGRHNAPFRVISSARMDPETVELELFGSEAEDGSIRVGLLEQAHGGTLFLDEVAD
MPLTTQGKILRVLTDQSFTRVGGRTMIRVDVRIISGSARDLTVEIAEGRFREDLYYRLNVVPVHIPSLRERRDDIASLCD
HFVRRYAADRRVPPPEISAEAMAALQAHDWPGNVRELRNVIERVMILAPSDRLGRIDADMLPAELVRGGADILPSSESIT
TIPLKEARENFEREYLRIQINRFSGNISRTASFIGMERSALHRKLKLLGLTESSDNESPA

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=35.4700854700855, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1788550, Length=459, Percent_Identity=34.4226579520697, Blast_Score=271, Evalue=8e-74,
Organism=Escherichia coli, GI1790437, Length=456, Percent_Identity=33.5526315789474, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI1788905, Length=453, Percent_Identity=34.878587196468, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1789233, Length=319, Percent_Identity=36.0501567398119, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1789087, Length=332, Percent_Identity=37.3493975903614, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI87082117, Length=341, Percent_Identity=36.950146627566, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1786524, Length=330, Percent_Identity=36.6666666666667, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87082152, Length=322, Percent_Identity=36.3354037267081, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI1787583, Length=324, Percent_Identity=34.8765432098765, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI87081872, Length=319, Percent_Identity=33.2288401253918, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87081858, Length=288, Percent_Identity=31.25, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789828, Length=260, Percent_Identity=32.6923076923077, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=33.0434782608696, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50838; Mature: 50706

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDILIVDDERDICELVAGVMEDEGYEARTASDSDSALEAIRQRRPSLALIDVWLQGSR
CEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCC
LDGLGLVEAIKAFDPTLPIIVISGHGGLDTAVAAIRRGAFDFIEKPFEASRLLHLVARAT
CCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHCC
ENERLKFEYEQLREKAGPAEELTGNSAAINNVRATLKRVAGTGSRVLITGAPGVGKEVAA
CCCCEEEHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RVLHGWSGRHNAPFRVISSARMDPETVELELFGSEAEDGSIRVGLLEQAHGGTLFLDEVA
HHHHCCCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCEEEEHHHC
DMPLTTQGKILRVLTDQSFTRVGGRTMIRVDVRIISGSARDLTVEIAEGRFREDLYYRLN
CCCCCCCCCEEEEECCCCHHHCCCEEEEEEEEEEEECCCCEEEEEECCCCHHCCEEEEEE
VVPVHIPSLRERRDDIASLCDHFVRRYAADRRVPPPEISAEAMAALQAHDWPGNVRELRN
EEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHH
VIERVMILAPSDRLGRIDADMLPAELVRGGADILPSSESITTIPLKEARENFEREYLRIQ
HHHHHHEECCCCCCCCCCHHCCHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHEEEEEE
INRFSGNISRTASFIGMERSALHRKLKLLGLTESSDNESPA
EEECCCCHHHHHHHHCCHHHHHHHHHHHEECCCCCCCCCCC
>Mature Secondary Structure 
ALDILIVDDERDICELVAGVMEDEGYEARTASDSDSALEAIRQRRPSLALIDVWLQGSR
EEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCC
LDGLGLVEAIKAFDPTLPIIVISGHGGLDTAVAAIRRGAFDFIEKPFEASRLLHLVARAT
CCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHCC
ENERLKFEYEQLREKAGPAEELTGNSAAINNVRATLKRVAGTGSRVLITGAPGVGKEVAA
CCCCEEEHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RVLHGWSGRHNAPFRVISSARMDPETVELELFGSEAEDGSIRVGLLEQAHGGTLFLDEVA
HHHHCCCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCEEEEHHHC
DMPLTTQGKILRVLTDQSFTRVGGRTMIRVDVRIISGSARDLTVEIAEGRFREDLYYRLN
CCCCCCCCCEEEEECCCCHHHCCCEEEEEEEEEEEECCCCEEEEEECCCCHHCCEEEEEE
VVPVHIPSLRERRDDIASLCDHFVRRYAADRRVPPPEISAEAMAALQAHDWPGNVRELRN
EEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHH
VIERVMILAPSDRLGRIDADMLPAELVRGGADILPSSESITTIPLKEARENFEREYLRIQ
HHHHHHEECCCCCCCCCCHHCCHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHEEEEEE
INRFSGNISRTASFIGMERSALHRKLKLLGLTESSDNESPA
EEECCCCHHHHHHHHCCHHHHHHHHHHHEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]