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The map label for this gene is nasA [H]

Identifier: 103486750

GI number: 103486750

Start: 1317017

End: 1318252

Strand: Direct

Name: nasA [H]

Synonym: Sala_1264

Alternate gene names: 103486750

Gene position: 1317017-1318252 (Clockwise)

Preceding gene: 103486749

Following gene: 103486751

Centisome position: 39.37

GC content: 67.48

Gene sequence:

>1236_bases
ATGACGACTGCAAGCGCGGGGGCCGGAGCGGCCCCCAAATCGAGTTTCTGGGCGAGCGGGCACTGGCCGACGCTCGTCGC
CGCCTTTCTCTATTTCGATCTCGCCTTCATGGTGTGGGTAATCCTCGGCCCGCTGGCGCCCGCGATTTCGGCCGACCTCG
GCCTGACCCCCGCGCAAAAGGGGTTGATGGTCGCAGTCCCCACGCTCGCCGGGGCGCTGCTCCGCGTCGTCAACGGCCTG
CTCGTCGATCGCATCGGCCCCAAGCGCGCCGGGGCGATCAGCCAGGTGATCGTAATCGCCGGGCTGTTCACCGCCTGGTT
GATGGGGGTGACCAGCTTCACCGGCACGCTCGCGCTCGGCGTGGTGCTCGGCTTCGCCGGGGCCAGCTTTGCGATTGCGC
TGCCGCTCGCGAGCCGCTGGTATCCGCCCGAGCATCAGGGCAAGGCGATGGGGCTCGCCGGGATGGGCAATTCGGGCACC
GTGCTCGCAGCGCTGTTCGCCCCCGGCCTCGCGGTGATGTTCGGCTGGAACGCGGTGCTCGGCCTCGCCTGCATCCCGCT
GGCGATCGTCTTTTTCCTCTATTCGTTCATGGCAAAAGACGCGCCGAACGCTCCTGCGCCCAAGAAGCTGGTCGACTATT
TCCAGCCGCTCAAGACCGCTGATGCCTGGTGGCTGATGGCCTTTTATTCGGTGACTTTCGGGGGCTTCGTCGGGCTGGCC
GCATCGCTGCCGATCTATTTCACCGACCAGTTCCATCTGAGCCCCGTGATGGCGGGCTATTGCACCGCCGCCTGCGTCTT
TGCCGGGTCGTTGGTGCGGCCGATGGGCGGCGCGCTCGCCGACCGGATCGGCGGGGTCAAGGCGCTGATGATGGTCTTTG
TCGTCGCGGCGCTAGCGCTCGCCGGGGTGCCGCAGGCGGACACCCTCGCCGCCGCGCTCGGCCTGTTCGTGATCGCGATG
CTCGCGCTCGGCACGGGGAATGGCTCGGTGTTCCAGCTCGTGCCGCAGCGTTTTTCGGCCGAGATCGGGGTGATGACCGG
GCTGGTCGGCATGGCGGGCGGCATCGGCGGCTTCTACCTCGCCTCCTCGCTCGGCTTTGCCAAGCAGTGGAGCGGCAGCT
TTGCGCCGGGCTTCTATATCTTCGCCGGGCTTGCCGTTGCCGCGCTCATCGCCCTGATGTTTGTCAAGAATCAGTGGCGC
GCAACCTGGGGCGCCGCCGAAGGCGTACGGATCTAG

Upstream 100 bases:

>100_bases
TTTTCCACATTCCGACATGGCAATGAAGCCGTCAGGATGCGCCCGCGCGGCCGCCGTCCTGGCGGCTTTTTTATTGCCCG
TCGCACGAAGGGGACGGACG

Downstream 100 bases:

>100_bases
CCATGACCCGCACCCTCATCCTTTTGCTGGCGCTCGCGCCGGCCGGGGCGGCACATGCCCAGGCCGTCGAGCTAGAGCCG
GTTGGCGAAGCCCGCCTCCG

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MTTASAGAGAAPKSSFWASGHWPTLVAAFLYFDLAFMVWVILGPLAPAISADLGLTPAQKGLMVAVPTLAGALLRVVNGL
LVDRIGPKRAGAISQVIVIAGLFTAWLMGVTSFTGTLALGVVLGFAGASFAIALPLASRWYPPEHQGKAMGLAGMGNSGT
VLAALFAPGLAVMFGWNAVLGLACIPLAIVFFLYSFMAKDAPNAPAPKKLVDYFQPLKTADAWWLMAFYSVTFGGFVGLA
ASLPIYFTDQFHLSPVMAGYCTAACVFAGSLVRPMGGALADRIGGVKALMMVFVVAALALAGVPQADTLAAALGLFVIAM
LALGTGNGSVFQLVPQRFSAEIGVMTGLVGMAGGIGGFYLASSLGFAKQWSGSFAPGFYIFAGLAVAALIALMFVKNQWR
ATWGAAEGVRI

Sequences:

>Translated_411_residues
MTTASAGAGAAPKSSFWASGHWPTLVAAFLYFDLAFMVWVILGPLAPAISADLGLTPAQKGLMVAVPTLAGALLRVVNGL
LVDRIGPKRAGAISQVIVIAGLFTAWLMGVTSFTGTLALGVVLGFAGASFAIALPLASRWYPPEHQGKAMGLAGMGNSGT
VLAALFAPGLAVMFGWNAVLGLACIPLAIVFFLYSFMAKDAPNAPAPKKLVDYFQPLKTADAWWLMAFYSVTFGGFVGLA
ASLPIYFTDQFHLSPVMAGYCTAACVFAGSLVRPMGGALADRIGGVKALMMVFVVAALALAGVPQADTLAAALGLFVIAM
LALGTGNGSVFQLVPQRFSAEIGVMTGLVGMAGGIGGFYLASSLGFAKQWSGSFAPGFYIFAGLAVAALIALMFVKNQWR
ATWGAAEGVRI
>Mature_410_residues
TTASAGAGAAPKSSFWASGHWPTLVAAFLYFDLAFMVWVILGPLAPAISADLGLTPAQKGLMVAVPTLAGALLRVVNGLL
VDRIGPKRAGAISQVIVIAGLFTAWLMGVTSFTGTLALGVVLGFAGASFAIALPLASRWYPPEHQGKAMGLAGMGNSGTV
LAALFAPGLAVMFGWNAVLGLACIPLAIVFFLYSFMAKDAPNAPAPKKLVDYFQPLKTADAWWLMAFYSVTFGGFVGLAA
SLPIYFTDQFHLSPVMAGYCTAACVFAGSLVRPMGGALADRIGGVKALMMVFVVAALALAGVPQADTLAAALGLFVIAML
ALGTGNGSVFQLVPQRFSAEIGVMTGLVGMAGGIGGFYLASSLGFAKQWSGSFAPGFYIFAGLAVAALIALMFVKNQWRA
TWGAAEGVRI

Specific function: May function as a nitrate transporter [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=394, Percent_Identity=26.3959390862944, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787743, Length=396, Percent_Identity=25.2525252525253, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42325; Mature: 42193

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTASAGAGAAPKSSFWASGHWPTLVAAFLYFDLAFMVWVILGPLAPAISADLGLTPAQK
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GLMVAVPTLAGALLRVVNGLLVDRIGPKRAGAISQVIVIAGLFTAWLMGVTSFTGTLALG
CCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVLGFAGASFAIALPLASRWYPPEHQGKAMGLAGMGNSGTVLAALFAPGLAVMFGWNAVL
HHHHHCCCHHHEEEHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHCHHHHHHHHHHHH
GLACIPLAIVFFLYSFMAKDAPNAPAPKKLVDYFQPLKTADAWWLMAFYSVTFGGFVGLA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASLPIYFTDQFHLSPVMAGYCTAACVFAGSLVRPMGGALADRIGGVKALMMVFVVAALAL
HCCCEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGVPQADTLAAALGLFVIAMLALGTGNGSVFQLVPQRFSAEIGVMTGLVGMAGGIGGFYL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ASSLGFAKQWSGSFAPGFYIFAGLAVAALIALMFVKNQWRATWGAAEGVRI
HHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTASAGAGAAPKSSFWASGHWPTLVAAFLYFDLAFMVWVILGPLAPAISADLGLTPAQK
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GLMVAVPTLAGALLRVVNGLLVDRIGPKRAGAISQVIVIAGLFTAWLMGVTSFTGTLALG
CCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVLGFAGASFAIALPLASRWYPPEHQGKAMGLAGMGNSGTVLAALFAPGLAVMFGWNAVL
HHHHHCCCHHHEEEHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHCHHHHHHHHHHHH
GLACIPLAIVFFLYSFMAKDAPNAPAPKKLVDYFQPLKTADAWWLMAFYSVTFGGFVGLA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASLPIYFTDQFHLSPVMAGYCTAACVFAGSLVRPMGGALADRIGGVKALMMVFVVAALAL
HCCCEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGVPQADTLAAALGLFVIAMLALGTGNGSVFQLVPQRFSAEIGVMTGLVGMAGGIGGFYL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ASSLGFAKQWSGSFAPGFYIFAGLAVAALIALMFVKNQWRATWGAAEGVRI
HHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]