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The map label for this gene is mltB [H]

Identifier: 103486740

GI number: 103486740

Start: 1306740

End: 1307804

Strand: Direct

Name: mltB [H]

Synonym: Sala_1254

Alternate gene names: 103486740

Gene position: 1306740-1307804 (Clockwise)

Preceding gene: 103486735

Following gene: 103486741

Centisome position: 39.06

GC content: 65.26

Gene sequence:

>1065_bases
ATGACGCAAACTAGCATTTTCATAATACGCAAATATTTCGTACTTTCAGCGGCAATACTGGGGGCGTGGATCGTTGCTGC
CCAGGCGCCGCTCCATGCGCAGAGCGCCGATGCCGGGTTCGACGCCTATGTCCAGTCGCTCTGGCCGAAAGCGCAGGCGC
GTGGCGTGTCGCGCGCGACGTTCGACCGGGTGACCGCCGGACTGCGCTACAATAGCCGGGTGGTGGCGCTCGACCGCGAC
AATCTGGGATCGCCGCCCAGCCCGAACACACCGATACCTGCTTTCGCGCCCTATAAGGCCCGGCACGTCGATGCCGCGCG
CATCAACGGCGGGCGCCGCGTCCACGACCGCCTGCTGCCGCTGCTCGCGCGCATCGAGGCACGAACGGGTGTGCCGACGA
GCATCATGATCGCAATTTACGGGCATGAGACCGCCTATGGCCAGGTCACGGGCAGCTTCGACCTGCCCGAAGCGCTCGCG
ACGCTGGCGTATGAGGGACGCCGACGCAGCCTGTTCGAGCCCGAGCTGATCGCGACGATGGTGATGGTCGAACGCGGCGT
GCCGCGCGATGTGCTGAAGGGGAGCTGGGCCGGCGCCTTCGGTTATCCGCAGTTCCTCCCTTCGGTCTATCTGCGTGTCG
CCGAGGACGGCGATGGCGACGGCGTGGCGCGGATATGGTCGAGCGAGGCCGATGCGATCGAGTCGATCGGCGCCTATCTG
CGCAACGCGGGCTGGCGCGCGGGTCAGCCATGGGGCGTTGCGGTCAACGTTCCGGCGACTTTCGATCGAGCCGCTGTTGC
CAACCGCCTGACCCCCACGCGCTGTCCGCGCGTGTTCGAGCGTCACAGCCGCTGGCGCTCGATGGCCGAGTGGCGCGCCG
CCGGGCTTCAGCCGGTCAGTGGGCGCTGGCCCGACGACGATGTTCAGGCGACCTTGCTCGAACCCGACGGGCCGGGGCGG
ACCGCTTATTTGCTCACCGGTAACTATCGTGCGATACTGGACTATAATTGTTCCAATTTTTACGCATTGTCTGTGGGATT
GCTGGCGGATGAAATCGATCGTTAG

Upstream 100 bases:

>100_bases
ACCTTGTTCCAACGGTCCGCCTTGCGAATGACTCGCCCTGTCCCGCGAGCGCCTAGCCAAGCGGCTCGATTCTGTGCGAA
ACCATCCCTATGGGTGGGTC

Downstream 100 bases:

>100_bases
GGACGGGTGCCTGATCGCGGGAGCAGCCCTGATGCTGTCCGGTTGCGGCGGGGTTGATGGCGCGCGCAGTGCGCGACCGG
TGCCCAACCCGGTCGCAGCA

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MTQTSIFIIRKYFVLSAAILGAWIVAAQAPLHAQSADAGFDAYVQSLWPKAQARGVSRATFDRVTAGLRYNSRVVALDRD
NLGSPPSPNTPIPAFAPYKARHVDAARINGGRRVHDRLLPLLARIEARTGVPTSIMIAIYGHETAYGQVTGSFDLPEALA
TLAYEGRRRSLFEPELIATMVMVERGVPRDVLKGSWAGAFGYPQFLPSVYLRVAEDGDGDGVARIWSSEADAIESIGAYL
RNAGWRAGQPWGVAVNVPATFDRAAVANRLTPTRCPRVFERHSRWRSMAEWRAAGLQPVSGRWPDDDVQATLLEPDGPGR
TAYLLTGNYRAILDYNCSNFYALSVGLLADEIDR

Sequences:

>Translated_354_residues
MTQTSIFIIRKYFVLSAAILGAWIVAAQAPLHAQSADAGFDAYVQSLWPKAQARGVSRATFDRVTAGLRYNSRVVALDRD
NLGSPPSPNTPIPAFAPYKARHVDAARINGGRRVHDRLLPLLARIEARTGVPTSIMIAIYGHETAYGQVTGSFDLPEALA
TLAYEGRRRSLFEPELIATMVMVERGVPRDVLKGSWAGAFGYPQFLPSVYLRVAEDGDGDGVARIWSSEADAIESIGAYL
RNAGWRAGQPWGVAVNVPATFDRAAVANRLTPTRCPRVFERHSRWRSMAEWRAAGLQPVSGRWPDDDVQATLLEPDGPGR
TAYLLTGNYRAILDYNCSNFYALSVGLLADEIDR
>Mature_353_residues
TQTSIFIIRKYFVLSAAILGAWIVAAQAPLHAQSADAGFDAYVQSLWPKAQARGVSRATFDRVTAGLRYNSRVVALDRDN
LGSPPSPNTPIPAFAPYKARHVDAARINGGRRVHDRLLPLLARIEARTGVPTSIMIAIYGHETAYGQVTGSFDLPEALAT
LAYEGRRRSLFEPELIATMVMVERGVPRDVLKGSWAGAFGYPQFLPSVYLRVAEDGDGDGVARIWSSEADAIESIGAYLR
NAGWRAGQPWGVAVNVPATFDRAAVANRLTPTRCPRVFERHSRWRSMAEWRAAGLQPVSGRWPDDDVQATLLEPDGPGRT
AYLLTGNYRAILDYNCSNFYALSVGLLADEIDR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=317, Percent_Identity=29.6529968454259, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 38804; Mature: 38673

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTSIFIIRKYFVLSAAILGAWIVAAQAPLHAQSADAGFDAYVQSLWPKAQARGVSRAT
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHCCCCHHH
FDRVTAGLRYNSRVVALDRDNLGSPPSPNTPIPAFAPYKARHVDAARINGGRRVHDRLLP
HHHHHHCCEECCEEEEEECCCCCCCCCCCCCCCCCCCCCHHCCCHHHCCCCCHHHHHHHH
LLARIEARTGVPTSIMIAIYGHETAYGQVTGSFDLPEALATLAYEGRRRSLFEPELIATM
HHHHHHHCCCCCCEEEEEEEECCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHH
VMVERGVPRDVLKGSWAGAFGYPQFLPSVYLRVAEDGDGDGVARIWSSEADAIESIGAYL
HHHHCCCCHHHHCCCCCCCCCCHHHHHHHHEEEECCCCCCCEEEHHCCHHHHHHHHHHHH
RNAGWRAGQPWGVAVNVPATFDRAAVANRLTPTRCPRVFERHSRWRSMAEWRAAGLQPVS
HHCCCCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GRWPDDDVQATLLEPDGPGRTAYLLTGNYRAILDYNCSNFYALSVGLLADEIDR
CCCCCCCCEEEEECCCCCCCEEEEEECCEEEEEECCCCCEEEEEHHHHHHHHCC
>Mature Secondary Structure 
TQTSIFIIRKYFVLSAAILGAWIVAAQAPLHAQSADAGFDAYVQSLWPKAQARGVSRAT
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHCCCCHHH
FDRVTAGLRYNSRVVALDRDNLGSPPSPNTPIPAFAPYKARHVDAARINGGRRVHDRLLP
HHHHHHCCEECCEEEEEECCCCCCCCCCCCCCCCCCCCCHHCCCHHHCCCCCHHHHHHHH
LLARIEARTGVPTSIMIAIYGHETAYGQVTGSFDLPEALATLAYEGRRRSLFEPELIATM
HHHHHHHCCCCCCEEEEEEEECCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHH
VMVERGVPRDVLKGSWAGAFGYPQFLPSVYLRVAEDGDGDGVARIWSSEADAIESIGAYL
HHHHCCCCHHHHCCCCCCCCCCHHHHHHHHEEEECCCCCCCEEEHHCCHHHHHHHHHHHH
RNAGWRAGQPWGVAVNVPATFDRAAVANRLTPTRCPRVFERHSRWRSMAEWRAAGLQPVS
HHCCCCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GRWPDDDVQATLLEPDGPGRTAYLLTGNYRAILDYNCSNFYALSVGLLADEIDR
CCCCCCCCEEEEECCCCCCCEEEEEECCEEEEEECCCCCEEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]