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The map label for this gene is fadA [H]

Identifier: 103486731

GI number: 103486731

Start: 1294604

End: 1295851

Strand: Reverse

Name: fadA [H]

Synonym: Sala_1244

Alternate gene names: 103486731

Gene position: 1295851-1294604 (Counterclockwise)

Preceding gene: 103486732

Following gene: 103486729

Centisome position: 38.74

GC content: 66.27

Gene sequence:

>1248_bases
ATGGCCGAAGCCTATATCATCGACGCCGTCCGCACCCCGCGCGGGATTGGCAAGCCCGGCAAGGGCGCGTTGTCGCATCT
CCACCCGCAGCATCTCGCCGCAACGGTGCTGAAGGCGATCAGGGATCGCAATAATCTCGACACCGCGACGGTCGACGACA
TCATCTGGTCGACGTCGAGCCAGAATGGCAAGCAGGGCGGCGACCTCGGCCGCATGGCGGCGCTGTCGGCGGGTTATGAC
ATCAAGGCATCGGGAACGACGCTCGACCGCTTCTGCGGCGGCGGCATCACCTCGGTCAATCTCGCCGCGGCGACGGTGAT
GAGCGGCATGGAGGATTGCGTCGTCGCGGGCGGCACCGAGATGATGAGCTACACCAGCGCCTTTGCCGCCGAACAGGCGA
ACGCCGGCCTGCCGCCGCGCCTGATGGGGTCGGGCCATGAGGCGCTCGACGAGCTTCACCCGCAGTCGCATCAGGGCGTG
TGCGGCGACGCCATCGCAACGATCGAAGGGATCGGCCGCGAGGCGCTCGATGCGCTCGCACTCGTCAGCCAGCGGCGCGC
CGACCGTGCGATCAAGGAAGGTCGCTTCGCCAAATCGCTGGTGCCCGTGCTCAACCCCGACGGCAGCATCGCACTCGACC
ATGAGGAGTTTCCGCGCCCCGAAACCAGCGCCGAAGGTCTCGCCGCGCTCAAGCCGAGTTTTGCCGCGCTCGCCGATTTC
GACATGGGCGGCTTCACCTTCCGCCAGCAGATCAATCGTCGCTATCCCGATCTCGAAATCCAGCATTTTCACCATGCCGG
CAATTCATCGGGGGTCGTCGATGGCGCTGCGGCGCTGCTCGTCACCTCGAAGGCCTATGCCGACAAACACGGCCTGAAAC
CCCGCGCGCGCATCGTCGCCTACGCCAATATCGGCGACGATCCGACCTTGATGCTCAACGCCCCGGTCCCCGCGGCGAAG
AAGGTGCTCGATAAGGCGGGGCTGACCAGGGACGACATCGACGTCTGGGAAATCAACGAGGCTTTCGCCGTCGTTGCGGA
GAAGTTCATCCGCGACCTCGACCTGCCCCGCGACAAGGTCAACATCAACGGCGGCGCGATGGCGCTCGGCCACCCGATCG
GCGCCACCGGATCGATCCTGATCGGCACCGCGCTCGACGAGCTGGAACGCTCGGGCGGTCGTTACGGCCTCGTCACCATG
TGCGCCGCCGGCGGCATGGCGCCCGCGATCATCATCGAGCGGCTGTGA

Upstream 100 bases:

>100_bases
GGAGATGATCCGCAATACCTATTTCAACGGTCAGGCCGTCCGCCTCGACGGCGCGATCCGCATGGCTCCGCGTTAACGCT
CCCCGAAAGGAAATATACAT

Downstream 100 bases:

>100_bases
TCAAAAGGGCCCCCGGATCGCGAGCGTTTTGGTCGGACTATAGAGGTTGACGAACATGGTCTTGCCGTCGGGCGAAAAAC
ACACGCCCGCCGGTTCGGTC

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MAEAYIIDAVRTPRGIGKPGKGALSHLHPQHLAATVLKAIRDRNNLDTATVDDIIWSTSSQNGKQGGDLGRMAALSAGYD
IKASGTTLDRFCGGGITSVNLAAATVMSGMEDCVVAGGTEMMSYTSAFAAEQANAGLPPRLMGSGHEALDELHPQSHQGV
CGDAIATIEGIGREALDALALVSQRRADRAIKEGRFAKSLVPVLNPDGSIALDHEEFPRPETSAEGLAALKPSFAALADF
DMGGFTFRQQINRRYPDLEIQHFHHAGNSSGVVDGAAALLVTSKAYADKHGLKPRARIVAYANIGDDPTLMLNAPVPAAK
KVLDKAGLTRDDIDVWEINEAFAVVAEKFIRDLDLPRDKVNINGGAMALGHPIGATGSILIGTALDELERSGGRYGLVTM
CAAGGMAPAIIIERL

Sequences:

>Translated_415_residues
MAEAYIIDAVRTPRGIGKPGKGALSHLHPQHLAATVLKAIRDRNNLDTATVDDIIWSTSSQNGKQGGDLGRMAALSAGYD
IKASGTTLDRFCGGGITSVNLAAATVMSGMEDCVVAGGTEMMSYTSAFAAEQANAGLPPRLMGSGHEALDELHPQSHQGV
CGDAIATIEGIGREALDALALVSQRRADRAIKEGRFAKSLVPVLNPDGSIALDHEEFPRPETSAEGLAALKPSFAALADF
DMGGFTFRQQINRRYPDLEIQHFHHAGNSSGVVDGAAALLVTSKAYADKHGLKPRARIVAYANIGDDPTLMLNAPVPAAK
KVLDKAGLTRDDIDVWEINEAFAVVAEKFIRDLDLPRDKVNINGGAMALGHPIGATGSILIGTALDELERSGGRYGLVTM
CAAGGMAPAIIIERL
>Mature_414_residues
AEAYIIDAVRTPRGIGKPGKGALSHLHPQHLAATVLKAIRDRNNLDTATVDDIIWSTSSQNGKQGGDLGRMAALSAGYDI
KASGTTLDRFCGGGITSVNLAAATVMSGMEDCVVAGGTEMMSYTSAFAAEQANAGLPPRLMGSGHEALDELHPQSHQGVC
GDAIATIEGIGREALDALALVSQRRADRAIKEGRFAKSLVPVLNPDGSIALDHEEFPRPETSAEGLAALKPSFAALADFD
MGGFTFRQQINRRYPDLEIQHFHHAGNSSGVVDGAAALLVTSKAYADKHGLKPRARIVAYANIGDDPTLMLNAPVPAAKK
VLDKAGLTRDDIDVWEINEAFAVVAEKFIRDLDLPRDKVNINGGAMALGHPIGATGSILIGTALDELERSGGRYGLVTMC
AAGGMAPAIIIERL

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=413, Percent_Identity=34.8668280871671, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI4501853, Length=421, Percent_Identity=35.1543942992874, Blast_Score=188, Evalue=9e-48,
Organism=Homo sapiens, GI167614485, Length=414, Percent_Identity=32.8502415458937, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI4557237, Length=434, Percent_Identity=30.6451612903226, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI4504327, Length=445, Percent_Identity=29.8876404494382, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI194353979, Length=89, Percent_Identity=52.8089887640449, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1787663, Length=428, Percent_Identity=40.1869158878505, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI48994986, Length=422, Percent_Identity=34.8341232227488, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI87082165, Length=422, Percent_Identity=35.5450236966825, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1788554, Length=424, Percent_Identity=36.7924528301887, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1788683, Length=419, Percent_Identity=26.0143198090692, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI133906874, Length=420, Percent_Identity=32.3809523809524, Blast_Score=186, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI25147385, Length=416, Percent_Identity=29.0865384615385, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17535921, Length=417, Percent_Identity=27.5779376498801, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17551802, Length=450, Percent_Identity=28.6666666666667, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17535917, Length=427, Percent_Identity=26.2295081967213, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6322031, Length=401, Percent_Identity=31.4214463840399, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325229, Length=423, Percent_Identity=30.0236406619385, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24655093, Length=419, Percent_Identity=35.3221957040573, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI17648125, Length=423, Percent_Identity=32.6241134751773, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24640423, Length=421, Percent_Identity=30.8788598574822, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI17137578, Length=446, Percent_Identity=28.9237668161435, Blast_Score=138, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 43606; Mature: 43475

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAYIIDAVRTPRGIGKPGKGALSHLHPQHLAATVLKAIRDRNNLDTATVDDIIWSTSS
CCCEEEEECHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCC
QNGKQGGDLGRMAALSAGYDIKASGTTLDRFCGGGITSVNLAAATVMSGMEDCVVAGGTE
CCCCCCCCHHHHHHHHCCCEEEECCCHHHHHHCCCCCHHHHHHHHHHHCHHHHHHCCCHH
MMSYTSAFAAEQANAGLPPRLMGSGHEALDELHPQSHQGVCGDAIATIEGIGREALDALA
HHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHH
LVSQRRADRAIKEGRFAKSLVPVLNPDGSIALDHEEFPRPETSAEGLAALKPSFAALADF
HHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEECHHHCCCCCCCCCCHHHHCCCHHHHHCC
DMGGFTFRQQINRRYPDLEIQHFHHAGNSSGVVDGAAALLVTSKAYADKHGLKPRARIVA
CCCCHHHHHHHHCCCCCCEEEHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEE
YANIGDDPTLMLNAPVPAAKKVLDKAGLTRDDIDVWEINEAFAVVAEKFIRDLDLPRDKV
EEECCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCEE
NINGGAMALGHPIGATGSILIGTALDELERSGGRYGLVTMCAAGGMAPAIIIERL
ECCCCEEEECCCCCCCCCEEEHHHHHHHHHCCCCEEHHHHHHCCCCCHHHHEECC
>Mature Secondary Structure 
AEAYIIDAVRTPRGIGKPGKGALSHLHPQHLAATVLKAIRDRNNLDTATVDDIIWSTSS
CCEEEEECHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCC
QNGKQGGDLGRMAALSAGYDIKASGTTLDRFCGGGITSVNLAAATVMSGMEDCVVAGGTE
CCCCCCCCHHHHHHHHCCCEEEECCCHHHHHHCCCCCHHHHHHHHHHHCHHHHHHCCCHH
MMSYTSAFAAEQANAGLPPRLMGSGHEALDELHPQSHQGVCGDAIATIEGIGREALDALA
HHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHH
LVSQRRADRAIKEGRFAKSLVPVLNPDGSIALDHEEFPRPETSAEGLAALKPSFAALADF
HHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEECHHHCCCCCCCCCCHHHHCCCHHHHHCC
DMGGFTFRQQINRRYPDLEIQHFHHAGNSSGVVDGAAALLVTSKAYADKHGLKPRARIVA
CCCCHHHHHHHHCCCCCCEEEHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEE
YANIGDDPTLMLNAPVPAAKKVLDKAGLTRDDIDVWEINEAFAVVAEKFIRDLDLPRDKV
EEECCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCEE
NINGGAMALGHPIGATGSILIGTALDELERSGGRYGLVTMCAAGGMAPAIIIERL
ECCCCEEEECCCCCCCCCEEEHHHHHHHHHCCCCEEHHHHHHCCCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]