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The map label for this gene is trmFO

Identifier: 103486647

GI number: 103486647

Start: 1192406

End: 1193755

Strand: Reverse

Name: trmFO

Synonym: Sala_1159

Alternate gene names: 103486647

Gene position: 1193755-1192406 (Counterclockwise)

Preceding gene: 103486649

Following gene: 103486645

Centisome position: 35.69

GC content: 65.85

Gene sequence:

>1350_bases
ATGGCGGACCACGACATTCACATCATCGGCGGAGGGCTCGCCGGGTCCGAAGCGGCGTGGCAGCTCGCCGAGGCGGGTTA
TCATGTGCGCCTCTCCGAAATGCGCGGCGGCGGCGACATGACTCCCGCGCATCAGGGCGACGGGCTTGCCGAAATGGTAT
GCTCGAACAGTTTTCGCAGCGACGACGGCGACAGCAACGCCGTCGGCCTTCTCCATCGCGAGATGCGCGCGCTGGGCTCG
ATCATCATGCGCGAAGCCGACGCGACCAAGGTGCCCGCCGGCTCGGCGCTCGCGGTCGACCGCGATCTTTTCTCGGGCGG
CGTGACGCGCGCGCTGTCGGAGCATCCCAATATCACCATCGTCCGCGAACGCATCGACAAACTCCCGACCGAAGGCCTCA
CCATCGTCGCGACCGGACCGCTCACCGCGGCATCGCTCGCGGAAAGCATCGGCGCTGCGACCGGCAAGGATGCGCTCGCC
TTTTTCGACGCCATCGCCCCCATCGTCTATCGCGACAGCATCGACATGGACATTGCGTGGATGGCGAGCCGCTGGGACAG
GGTCGGGCCGATCGGCGATGGCAGGGACTATATCAACTGTCCGCTGGACAAGGATCAGTATTACGCCTTCGTCCAGGGGC
TGCTCGATGGTGAAAAGACCGAGTTCAAGGATTGGGAAAAGGACACGCCCTATTTCGAGGGCTGCATGCCGATCGAGGTG
ATGGCCGAACGCGGGGTGGAGACGCTGCGCTTCGGCCCGATGAAGGGCGTCGGGCTCGACAATCCGCGCACCGGACGCTG
GCCCTATGCGGTCGTCCAGTTGCGGCAGGACAATGCGCTGGGCACGCTCTGGAACATGGTGGGTTTCCAGACCAAGCTCA
AACATGCCGAGCAGGTCCGGCTGTTCCGCACCATACCGGGGCTGGAAAAGGCCGAGTTCGCGAGGTTGGGTGGGCTTCAC
CGCAACAGCTTCATCCGCTCGCCCGAATTGCTCGACCGGCAGCTTCGCTTGAAGTCGGCGCCGCACATCCGCTTCGCGGG
GCAGATAACCGGCTGCGAGGGCTATGTCGAAAGCGCCGCGATCGGCCTCGTCGCCGCGCGCTTCGCCGCCGCCGAACTTG
GCGGGCGCGCCCTGCCCCCGCCACCGCCCGAAACCGCGCTCGGCGCGCTGCTCTGCCACATCACCGGCGGCGCCGATGCG
TCATCCTATCAGCCGATGAACGTCAATTTCGGGCTTTTTCCACCGCTGGCGGACGATGTGCGGAAGAAAGACCGCAAGCT
CGGCTATACGCAGCGCGCGGGCAAGGCGCTGGCGGAGTGGATGACGGTTGCGGCGGGTGTGGCCGCCTGA

Upstream 100 bases:

>100_bases
GATCCCAGCCCTGCTCTCCGTTCATCGCCCGCCCCTTTGCATCTTGCCGCCAACCGGCCTAGTGCGCGGCGGATCAAGCG
ATAGTCAATGGAAACGGTAA

Downstream 100 bases:

>100_bases
ACCATGTTCGTCATTGCGAGGAGCCGAGGGCGACGCAGCAATCCGGAGTGGCGTAAAACGCCCTGGATTGGTTCGCTCCG
CTCGCAATGACGAAACTGGT

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MADHDIHIIGGGLAGSEAAWQLAEAGYHVRLSEMRGGGDMTPAHQGDGLAEMVCSNSFRSDDGDSNAVGLLHREMRALGS
IIMREADATKVPAGSALAVDRDLFSGGVTRALSEHPNITIVRERIDKLPTEGLTIVATGPLTAASLAESIGAATGKDALA
FFDAIAPIVYRDSIDMDIAWMASRWDRVGPIGDGRDYINCPLDKDQYYAFVQGLLDGEKTEFKDWEKDTPYFEGCMPIEV
MAERGVETLRFGPMKGVGLDNPRTGRWPYAVVQLRQDNALGTLWNMVGFQTKLKHAEQVRLFRTIPGLEKAEFARLGGLH
RNSFIRSPELLDRQLRLKSAPHIRFAGQITGCEGYVESAAIGLVAARFAAAELGGRALPPPPPETALGALLCHITGGADA
SSYQPMNVNFGLFPPLADDVRKKDRKLGYTQRAGKALAEWMTVAAGVAA

Sequences:

>Translated_449_residues
MADHDIHIIGGGLAGSEAAWQLAEAGYHVRLSEMRGGGDMTPAHQGDGLAEMVCSNSFRSDDGDSNAVGLLHREMRALGS
IIMREADATKVPAGSALAVDRDLFSGGVTRALSEHPNITIVRERIDKLPTEGLTIVATGPLTAASLAESIGAATGKDALA
FFDAIAPIVYRDSIDMDIAWMASRWDRVGPIGDGRDYINCPLDKDQYYAFVQGLLDGEKTEFKDWEKDTPYFEGCMPIEV
MAERGVETLRFGPMKGVGLDNPRTGRWPYAVVQLRQDNALGTLWNMVGFQTKLKHAEQVRLFRTIPGLEKAEFARLGGLH
RNSFIRSPELLDRQLRLKSAPHIRFAGQITGCEGYVESAAIGLVAARFAAAELGGRALPPPPPETALGALLCHITGGADA
SSYQPMNVNFGLFPPLADDVRKKDRKLGYTQRAGKALAEWMTVAAGVAA
>Mature_448_residues
ADHDIHIIGGGLAGSEAAWQLAEAGYHVRLSEMRGGGDMTPAHQGDGLAEMVCSNSFRSDDGDSNAVGLLHREMRALGSI
IMREADATKVPAGSALAVDRDLFSGGVTRALSEHPNITIVRERIDKLPTEGLTIVATGPLTAASLAESIGAATGKDALAF
FDAIAPIVYRDSIDMDIAWMASRWDRVGPIGDGRDYINCPLDKDQYYAFVQGLLDGEKTEFKDWEKDTPYFEGCMPIEVM
AERGVETLRFGPMKGVGLDNPRTGRWPYAVVQLRQDNALGTLWNMVGFQTKLKHAEQVRLFRTIPGLEKAEFARLGGLHR
NSFIRSPELLDRQLRLKSAPHIRFAGQITGCEGYVESAAIGLVAARFAAAELGGRALPPPPPETALGALLCHITGGADAS
SYQPMNVNFGLFPPLADDVRKKDRKLGYTQRAGKALAEWMTVAAGVAA

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_SPHAL (Q1GTZ7)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616208.1
- ProteinModelPortal:   Q1GTZ7
- SMR:   Q1GTZ7
- GeneID:   4080865
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_1159
- NMPDR:   fig|317655.9.peg.1093
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   SALA317655:SALA_1159-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 48462; Mature: 48331

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADHDIHIIGGGLAGSEAAWQLAEAGYHVRLSEMRGGGDMTPAHQGDGLAEMVCSNSFRS
CCCCCEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCC
DDGDSNAVGLLHREMRALGSIIMREADATKVPAGSALAVDRDLFSGGVTRALSEHPNITI
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHCHHHHHHHCCCCEEE
VRERIDKLPTEGLTIVATGPLTAASLAESIGAATGKDALAFFDAIAPIVYRDSIDMDIAW
EHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHH
MASRWDRVGPIGDGRDYINCPLDKDQYYAFVQGLLDGEKTEFKDWEKDTPYFEGCMPIEV
HHHHCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCHHHH
MAERGVETLRFGPMKGVGLDNPRTGRWPYAVVQLRQDNALGTLWNMVGFQTKLKHAEQVR
HHHCCCHHEECCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
LFRTIPGLEKAEFARLGGLHRNSFIRSPELLDRQLRLKSAPHIRFAGQITGCEGYVESAA
HHHHCCCCCHHHHHHHCCCHHHHCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
IGLVAARFAAAELGGRALPPPPPETALGALLCHITGGADASSYQPMNVNFGLFPPLADDV
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHH
RKKDRKLGYTQRAGKALAEWMTVAAGVAA
HHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADHDIHIIGGGLAGSEAAWQLAEAGYHVRLSEMRGGGDMTPAHQGDGLAEMVCSNSFRS
CCCCEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCC
DDGDSNAVGLLHREMRALGSIIMREADATKVPAGSALAVDRDLFSGGVTRALSEHPNITI
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHCHHHHHHHCCCCEEE
VRERIDKLPTEGLTIVATGPLTAASLAESIGAATGKDALAFFDAIAPIVYRDSIDMDIAW
EHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHH
MASRWDRVGPIGDGRDYINCPLDKDQYYAFVQGLLDGEKTEFKDWEKDTPYFEGCMPIEV
HHHHCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCHHHH
MAERGVETLRFGPMKGVGLDNPRTGRWPYAVVQLRQDNALGTLWNMVGFQTKLKHAEQVR
HHHCCCHHEECCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
LFRTIPGLEKAEFARLGGLHRNSFIRSPELLDRQLRLKSAPHIRFAGQITGCEGYVESAA
HHHHCCCCCHHHHHHHCCCHHHHCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
IGLVAARFAAAELGGRALPPPPPETALGALLCHITGGADASSYQPMNVNFGLFPPLADDV
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHH
RKKDRKLGYTQRAGKALAEWMTVAAGVAA
HHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA