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The map label for this gene is ampR [H]

Identifier: 103486577

GI number: 103486577

Start: 1116776

End: 1117567

Strand: Direct

Name: ampR [H]

Synonym: Sala_1088

Alternate gene names: 103486577

Gene position: 1116776-1117567 (Clockwise)

Preceding gene: 103486576

Following gene: 103486578

Centisome position: 33.38

GC content: 69.57

Gene sequence:

>792_bases
ATGAAGCGCACGCACCTGCCCCTCAACGCGCTCCGCGTCTTCGATGCCGCTGCCCGGCATTTGAGTTTCACGCGCGCCGC
CGACGAGCTGGCGGTGACGCCCGCGGCGGTCGGGCAGCAGATTCGCGCGCTGGAGGATATGCTCGGCGTCGTGCTGTTCC
GCCGCACGCCCAAGGGGCTCGAACTGACCGGTGAAGCGCAGGCCGGGCTCGACGCGCTGCGGCAGGGATTCTTGCAGTTC
GAGGAATCGGTGCGCGCGATGCAGGCAGGGCAATCGTCGCAGTCGCTGACCATTGCCGCGCCGCGCGACCTGACCGCCAA
ATGGCTCGCCCCGCGGCTCGCGCGCTACAGCCAGCAGGCGCCCGACGTGCGCTTCACGCTGGTTTCGGCCGATACCGGCA
TCGATTTCACCGAAGCGAACCTCGACCTTGCGATCTTCTGGACCGATAGTGCGGGCGACCATGAGGGCGTGGCGCTGTCG
GAGCCGTTGATGGTCACCGTCGCGGGACCGGGAGGGGGCAGCGATACGCGCATTGCCTGGCCCGGCTGTCCGGTCGCCGA
TGGCGAACCCGCGCTGCGGCTGGGCGATGCGGGGCTGGCAATTGATGCCGCCGCGAACGGGCTGGGTCATGCCAATGTCC
CCGCAATGCTCGCCGAAGGAGACATCGCCGCGGGTCGCGTCCGGCAGGTGGGCGAGCCGGCGCCGACCAAGCGCGGCTAT
TGGCTCGTCGCGCCGACCCCGCAATGGCGGCAGGCGAAGGTCAAGGCGCTGGTTGCCGCGTTGACGGCATAA

Upstream 100 bases:

>100_bases
AAGGGTCGATGGTCGGCAGCTATACGATGGAGATGAGCGACGGATCGCAGATGCTCGTCGCGATCCCGCACTTCCCTCTG
GTGGCGCCCGCGACGGCATC

Downstream 100 bases:

>100_bases
TAACTTCTATTGCGCGCGTCATCCCCCTACGCCAATATCACTACTTGGCGAAGGGGGATGAGTATGGATCAGCGTGATGT
CCGGTCTCAGCTATATTTTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKRTHLPLNALRVFDAAARHLSFTRAADELAVTPAAVGQQIRALEDMLGVVLFRRTPKGLELTGEAQAGLDALRQGFLQF
EESVRAMQAGQSSQSLTIAAPRDLTAKWLAPRLARYSQQAPDVRFTLVSADTGIDFTEANLDLAIFWTDSAGDHEGVALS
EPLMVTVAGPGGGSDTRIAWPGCPVADGEPALRLGDAGLAIDAAANGLGHANVPAMLAEGDIAAGRVRQVGEPAPTKRGY
WLVAPTPQWRQAKVKALVAALTA

Sequences:

>Translated_263_residues
MKRTHLPLNALRVFDAAARHLSFTRAADELAVTPAAVGQQIRALEDMLGVVLFRRTPKGLELTGEAQAGLDALRQGFLQF
EESVRAMQAGQSSQSLTIAAPRDLTAKWLAPRLARYSQQAPDVRFTLVSADTGIDFTEANLDLAIFWTDSAGDHEGVALS
EPLMVTVAGPGGGSDTRIAWPGCPVADGEPALRLGDAGLAIDAAANGLGHANVPAMLAEGDIAAGRVRQVGEPAPTKRGY
WLVAPTPQWRQAKVKALVAALTA
>Mature_263_residues
MKRTHLPLNALRVFDAAARHLSFTRAADELAVTPAAVGQQIRALEDMLGVVLFRRTPKGLELTGEAQAGLDALRQGFLQF
EESVRAMQAGQSSQSLTIAAPRDLTAKWLAPRLARYSQQAPDVRFTLVSADTGIDFTEANLDLAIFWTDSAGDHEGVALS
EPLMVTVAGPGGGSDTRIAWPGCPVADGEPALRLGDAGLAIDAAANGLGHANVPAMLAEGDIAAGRVRQVGEPAPTKRGY
WLVAPTPQWRQAKVKALVAALTA

Specific function: This protein is a positive regulator of gene expression of beta-lactamase (AmpC) [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=278, Percent_Identity=31.6546762589928, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1786448, Length=270, Percent_Identity=27.037037037037, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788706, Length=143, Percent_Identity=30.7692307692308, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 27734; Mature: 27734

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTHLPLNALRVFDAAARHLSFTRAADELAVTPAAVGQQIRALEDMLGVVLFRRTPKGL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
ELTGEAQAGLDALRQGFLQFEESVRAMQAGQSSQSLTIAAPRDLTAKWLAPRLARYSQQA
EECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCC
PDVRFTLVSADTGIDFTEANLDLAIFWTDSAGDHEGVALSEPLMVTVAGPGGGSDTRIAW
CCEEEEEEECCCCCEEEECCEEEEEEEECCCCCCCCEEECCCEEEEEECCCCCCCCEEEC
PGCPVADGEPALRLGDAGLAIDAAANGLGHANVPAMLAEGDIAAGRVRQVGEPAPTKRGY
CCCCCCCCCCCEEECCCCEEEEHHCCCCCCCCCCEEEECCCCCHHHHHHCCCCCCCCCCE
WLVAPTPQWRQAKVKALVAALTA
EEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRTHLPLNALRVFDAAARHLSFTRAADELAVTPAAVGQQIRALEDMLGVVLFRRTPKGL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
ELTGEAQAGLDALRQGFLQFEESVRAMQAGQSSQSLTIAAPRDLTAKWLAPRLARYSQQA
EECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCC
PDVRFTLVSADTGIDFTEANLDLAIFWTDSAGDHEGVALSEPLMVTVAGPGGGSDTRIAW
CCEEEEEEECCCCCEEEECCEEEEEEEECCCCCCCCEEECCCEEEEEECCCCCCCCEEEC
PGCPVADGEPALRLGDAGLAIDAAANGLGHANVPAMLAEGDIAAGRVRQVGEPAPTKRGY
CCCCCCCCCCCEEECCCCEEEEHHCCCCCCCCCCEEEECCCCCHHHHHHCCCCCCCCCCE
WLVAPTPQWRQAKVKALVAALTA
EEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8064842 [H]