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The map label for this gene is cysK [H]

Identifier: 103486534

GI number: 103486534

Start: 1077650

End: 1078570

Strand: Reverse

Name: cysK [H]

Synonym: Sala_1045

Alternate gene names: 103486534

Gene position: 1078570-1077650 (Counterclockwise)

Preceding gene: 103486535

Following gene: 103486533

Centisome position: 32.24

GC content: 64.17

Gene sequence:

>921_bases
ATGAAAGCCAATTCCATCCTCGAAACCATCGGCAACACGCCGCACATTCGCATGGCAAAGCTTTTTCCCGACGCCGAAGT
CTGGGTAAAATCGGAACGCAGCAACCCCGGCGGCTCGATCAAGGACCGCATCGGCCTGGCGATGATCGAGGCGGCCGAAC
GCGACGGCAGCCTGAAGGAAGGCGGAACGATCGTCGAGCCGACCTCGGGCAATACCGGCATCGGACTTGCGATGGCCGCG
GCGGTCAAGGGCTACAAGCTCGTGCTCGTCATGCCCGAATCGATGTCGCTCGAACGCCGTCGGCTGATGCTTGCCTATGG
CGCGACCTTTGACCTCACGCCGCGCGAAAAGGGCATGAAGGGAGCGATCGAGCGCGCGATCGAACTCGTCGAGACGACCC
CCGGCGCCTGGATGCCGCAGCAGTTCGAAAATGAAGCCAACATCGACGTCCATATCCGCACCACCGGCCCCGAAATCCTT
GCCGATTTCCGGGATACGCCGATCGACGTGCTCGTCACCGGCGTCGGCACCGGCGGCCATATCACCGGCTGCGCGCAATT
CCTGAAAGAGCATTGGCCGAACCTCAAGGTCTTCGCGGTCGAACCCGAAGCCTCACCGGTCATTTCGGGCGGCCAGCCCG
CGCCGCACCCGATCCAGGGTATCGGTGCCGGCTTCATCCCGCGCAACCTCCACACCGACCTGCTCGACGGCGTCATCAAG
GTCGATGCTTCCGCCGCAAAGGACATGGCACGCCGCGCCGCGACCGAGGAAGGGATGCTCGTCGGCATCTCGTCGGGCGC
CACGCTCGCCGCGATCGCGCAGAAGCTCGCCGAACTGCCGCCGGGCAGCCGCGTACTCGGTTTCAACTACGACACCGGCG
AGCGTTATCTGTCGGTGCCCGACTTCCTGCCCGAAATCTGA

Upstream 100 bases:

>100_bases
GCGGTGCTGGCGCGATCGGCGTGACGGCGCTATGGGCGTGGATATTTCCCGAACTGCGCCGCGCGCGTACCTTTGAACCT
CAATTCAAGGCAGGATCGAC

Downstream 100 bases:

>100_bases
CCTTCCGCCATGGGCGCTGCAAGCGATCAGCCGGCGCGGCGGCGGCACGACTGGACGGGCTGGCTGTTCGTCCTGCTCGC
GATGCTTGCGATCGGCGGTG

Product: cysteine synthases

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKANSILETIGNTPHIRMAKLFPDAEVWVKSERSNPGGSIKDRIGLAMIEAAERDGSLKEGGTIVEPTSGNTGIGLAMAA
AVKGYKLVLVMPESMSLERRRLMLAYGATFDLTPREKGMKGAIERAIELVETTPGAWMPQQFENEANIDVHIRTTGPEIL
ADFRDTPIDVLVTGVGTGGHITGCAQFLKEHWPNLKVFAVEPEASPVISGGQPAPHPIQGIGAGFIPRNLHTDLLDGVIK
VDASAAKDMARRAATEEGMLVGISSGATLAAIAQKLAELPPGSRVLGFNYDTGERYLSVPDFLPEI

Sequences:

>Translated_306_residues
MKANSILETIGNTPHIRMAKLFPDAEVWVKSERSNPGGSIKDRIGLAMIEAAERDGSLKEGGTIVEPTSGNTGIGLAMAA
AVKGYKLVLVMPESMSLERRRLMLAYGATFDLTPREKGMKGAIERAIELVETTPGAWMPQQFENEANIDVHIRTTGPEIL
ADFRDTPIDVLVTGVGTGGHITGCAQFLKEHWPNLKVFAVEPEASPVISGGQPAPHPIQGIGAGFIPRNLHTDLLDGVIK
VDASAAKDMARRAATEEGMLVGISSGATLAAIAQKLAELPPGSRVLGFNYDTGERYLSVPDFLPEI
>Mature_306_residues
MKANSILETIGNTPHIRMAKLFPDAEVWVKSERSNPGGSIKDRIGLAMIEAAERDGSLKEGGTIVEPTSGNTGIGLAMAA
AVKGYKLVLVMPESMSLERRRLMLAYGATFDLTPREKGMKGAIERAIELVETTPGAWMPQQFENEANIDVHIRTTGPEIL
ADFRDTPIDVLVTGVGTGGHITGCAQFLKEHWPNLKVFAVEPEASPVISGGQPAPHPIQGIGAGFIPRNLHTDLLDGVIK
VDASAAKDMARRAATEEGMLVGISSGATLAAIAQKLAELPPGSRVLGFNYDTGERYLSVPDFLPEI

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=305, Percent_Identity=40.327868852459, Blast_Score=198, Evalue=5e-51,
Organism=Homo sapiens, GI295821200, Length=305, Percent_Identity=40.327868852459, Blast_Score=198, Evalue=5e-51,
Organism=Homo sapiens, GI4557415, Length=305, Percent_Identity=40.327868852459, Blast_Score=198, Evalue=5e-51,
Organism=Escherichia coli, GI1788754, Length=305, Percent_Identity=48.5245901639344, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI2367138, Length=301, Percent_Identity=38.8704318936877, Blast_Score=198, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI115535073, Length=308, Percent_Identity=49.6753246753247, Blast_Score=234, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI17535051, Length=298, Percent_Identity=45.9731543624161, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17562970, Length=309, Percent_Identity=42.0711974110032, Blast_Score=218, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI32566674, Length=297, Percent_Identity=43.0976430976431, Blast_Score=218, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17561720, Length=296, Percent_Identity=41.8918918918919, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17534315, Length=313, Percent_Identity=38.6581469648562, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI32566672, Length=223, Percent_Identity=40.8071748878924, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI25147552, Length=282, Percent_Identity=37.9432624113475, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17561716, Length=115, Percent_Identity=41.7391304347826, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6321594, Length=295, Percent_Identity=34.9152542372881, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6321449, Length=323, Percent_Identity=34.0557275541796, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24643623, Length=309, Percent_Identity=37.5404530744337, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI20129101, Length=309, Percent_Identity=37.5404530744337, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32646; Mature: 32646

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKANSILETIGNTPHIRMAKLFPDAEVWVKSERSNPGGSIKDRIGLAMIEAAERDGSLKE
CCHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCC
GGTIVEPTSGNTGIGLAMAAAVKGYKLVLVMPESMSLERRRLMLAYGATFDLTPREKGMK
CCEEEECCCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHEEEEEECCEEECCCHHCCHH
GAIERAIELVETTPGAWMPQQFENEANIDVHIRTTGPEILADFRDTPIDVLVTGVGTGGH
HHHHHHHHHHHCCCCCCCCHHHCCCCCEEEEEEECCHHHHHHCCCCCEEEEEEECCCCCH
ITGCAQFLKEHWPNLKVFAVEPEASPVISGGQPAPHPIQGIGAGFIPRNLHTDLLDGVIK
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHE
VDASAAKDMARRAATEEGMLVGISSGATLAAIAQKLAELPPGSRVLGFNYDTGERYLSVP
ECHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCEEECC
DFLPEI
HHCCCC
>Mature Secondary Structure
MKANSILETIGNTPHIRMAKLFPDAEVWVKSERSNPGGSIKDRIGLAMIEAAERDGSLKE
CCHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCC
GGTIVEPTSGNTGIGLAMAAAVKGYKLVLVMPESMSLERRRLMLAYGATFDLTPREKGMK
CCEEEECCCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHEEEEEECCEEECCCHHCCHH
GAIERAIELVETTPGAWMPQQFENEANIDVHIRTTGPEILADFRDTPIDVLVTGVGTGGH
HHHHHHHHHHHCCCCCCCCHHHCCCCCEEEEEEECCHHHHHHCCCCCEEEEEEECCCCCH
ITGCAQFLKEHWPNLKVFAVEPEASPVISGGQPAPHPIQGIGAGFIPRNLHTDLLDGVIK
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHE
VDASAAKDMARRAATEEGMLVGISSGATLAAIAQKLAELPPGSRVLGFNYDTGERYLSVP
ECHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCEEECC
DFLPEI
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8867384 [H]