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The map label for this gene is lacF [H]

Identifier: 103486511

GI number: 103486511

Start: 1055449

End: 1056333

Strand: Reverse

Name: lacF [H]

Synonym: Sala_1022

Alternate gene names: 103486511

Gene position: 1056333-1055449 (Counterclockwise)

Preceding gene: 103486512

Following gene: 103486510

Centisome position: 31.58

GC content: 63.39

Gene sequence:

>885_bases
ATGCGCCCCCGCGAAGCGCGCGCCGGATGGGCGATGAGCGCGCCGGCGCTCGCCGCGATACTCGTGTTCTTCGTCGTTCC
CGCGATGGGTTCCTTGTTCCTCAGCTTCACCGACTTCGACATCTATGCGCTCGCCGACCTCGACAACCTGCGCTTCGTCG
GTTTCCAGAATTATGAAAGACTCCTCGCCAACCCCTTGTTCTGGAAGGCGACGACGAACACATTGCTGTTCGTCGTTATC
GGCACTCCCTTCATCACCCTGTTGTCGCTCTTCGCTGCCATACTCGTCAACTCGCGCTGGCTCGCGTGGCGACCGGTGTG
GCGTGTCGCCTTGTTCGCACCCTATGTGACGACGCTCGTGGCTACGGCCGTCGTGTGGCGCTATCTGCTCCACACGCGCT
ACGGCCTCGTCAACTATCTGCTCTCGCTCTTCGGTATCGCGCCGATCGACTGGCTCGGCAGTCCGACCGCGTCGCTGCCC
GCGATCCTGATCTTCGTCGGCTGGAAGACCTTCGGTTATAACATGATCATCTTCCTCGCCGCGCTCCAGACGGTGCCGCG
CGAGCTTGACGAAGCGGCGCGCATCGATGGCGCCCGCTGGTTCACGCGCCTGCGCCACGTCACCCTGCCCGCGATCGCGC
CGACGGTGCTGCTGGTGTCGGTGCTGACCGTTGCGGGCATGTTCCAGCTTTTCGCCGAACCCTATGTGATGACGCAGGGC
GGTCCCGCCCAGTCGACCGTGACCATCCTCTATTTCATGTACGAGGAAGGGTTCAAATGGTGGAACCTGGGGTCGGGCGC
CGCGGTGGCCTTCCTGCTCTTTCTCTGCATCCTCGCAGTGACCCTGGCGCAGCTCCGGCTGGCAAGACGGAGCGGCGCGA
TATGA

Upstream 100 bases:

>100_bases
GCGTCGATGCCGCCGCGCACGAGATCGACCGCCGCGCCGACCGCCTGCTCGAAAAAAGGCGCTGGATGCTCGACAGGGGG
CGCGCCCTGTGACCGGCGCG

Downstream 100 bases:

>100_bases
GCCTTCGCCGCTGGACCGTCACGCTGCTCGCCGCATTCGTCGCGCTGCTCACCATCGCGCCGCTGCTCTGGATGCTGTCG
GTCAGCTTCATGGCGCGCGG

Product: binding-protein-dependent transport systems inner membrane component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MRPREARAGWAMSAPALAAILVFFVVPAMGSLFLSFTDFDIYALADLDNLRFVGFQNYERLLANPLFWKATTNTLLFVVI
GTPFITLLSLFAAILVNSRWLAWRPVWRVALFAPYVTTLVATAVVWRYLLHTRYGLVNYLLSLFGIAPIDWLGSPTASLP
AILIFVGWKTFGYNMIIFLAALQTVPRELDEAARIDGARWFTRLRHVTLPAIAPTVLLVSVLTVAGMFQLFAEPYVMTQG
GPAQSTVTILYFMYEEGFKWWNLGSGAAVAFLLFLCILAVTLAQLRLARRSGAI

Sequences:

>Translated_294_residues
MRPREARAGWAMSAPALAAILVFFVVPAMGSLFLSFTDFDIYALADLDNLRFVGFQNYERLLANPLFWKATTNTLLFVVI
GTPFITLLSLFAAILVNSRWLAWRPVWRVALFAPYVTTLVATAVVWRYLLHTRYGLVNYLLSLFGIAPIDWLGSPTASLP
AILIFVGWKTFGYNMIIFLAALQTVPRELDEAARIDGARWFTRLRHVTLPAIAPTVLLVSVLTVAGMFQLFAEPYVMTQG
GPAQSTVTILYFMYEEGFKWWNLGSGAAVAFLLFLCILAVTLAQLRLARRSGAI
>Mature_294_residues
MRPREARAGWAMSAPALAAILVFFVVPAMGSLFLSFTDFDIYALADLDNLRFVGFQNYERLLANPLFWKATTNTLLFVVI
GTPFITLLSLFAAILVNSRWLAWRPVWRVALFAPYVTTLVATAVVWRYLLHTRYGLVNYLLSLFGIAPIDWLGSPTASLP
AILIFVGWKTFGYNMIIFLAALQTVPRELDEAARIDGARWFTRLRHVTLPAIAPTVLLVSVLTVAGMFQLFAEPYVMTQG
GPAQSTVTILYFMYEEGFKWWNLGSGAAVAFLLFLCILAVTLAQLRLARRSGAI

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787570, Length=246, Percent_Identity=35.7723577235772, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789861, Length=276, Percent_Identity=32.6086956521739, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1790465, Length=229, Percent_Identity=29.2576419213974, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32827; Mature: 32827

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPREARAGWAMSAPALAAILVFFVVPAMGSLFLSFTDFDIYALADLDNLRFVGFQNYER
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECHHHHHH
LLANPLFWKATTNTLLFVVIGTPFITLLSLFAAILVNSRWLAWRPVWRVALFAPYVTTLV
HHCCCCEEEECCCEEEEEEECCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHH
ATAVVWRYLLHTRYGLVNYLLSLFGIAPIDWLGSPTASLPAILIFVGWKTFGYNMIIFLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALQTVPRELDEAARIDGARWFTRLRHVTLPAIAPTVLLVSVLTVAGMFQLFAEPYVMTQG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
GPAQSTVTILYFMYEEGFKWWNLGSGAAVAFLLFLCILAVTLAQLRLARRSGAI
CCCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRPREARAGWAMSAPALAAILVFFVVPAMGSLFLSFTDFDIYALADLDNLRFVGFQNYER
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECHHHHHH
LLANPLFWKATTNTLLFVVIGTPFITLLSLFAAILVNSRWLAWRPVWRVALFAPYVTTLV
HHCCCCEEEECCCEEEEEEECCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHH
ATAVVWRYLLHTRYGLVNYLLSLFGIAPIDWLGSPTASLPAILIFVGWKTFGYNMIIFLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALQTVPRELDEAARIDGARWFTRLRHVTLPAIAPTVLLVSVLTVAGMFQLFAEPYVMTQG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
GPAQSTVTILYFMYEEGFKWWNLGSGAAVAFLLFLCILAVTLAQLRLARRSGAI
CCCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]