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The map label for this gene is fadK [H]

Identifier: 103486496

GI number: 103486496

Start: 1031852

End: 1033567

Strand: Reverse

Name: fadK [H]

Synonym: Sala_1007

Alternate gene names: 103486496

Gene position: 1033567-1031852 (Counterclockwise)

Preceding gene: 103486497

Following gene: 103486495

Centisome position: 30.9

GC content: 66.96

Gene sequence:

>1716_bases
ATGATCGTCGCGGGAGAGGATAAACGGCGGCGTTATCGGCAAAGCGGCTGGTGGGGCGACCAGAGCTTTGCCGACCTGTT
CGCGGCCAATGCGGCGGCGCATCCCGACCGGCTCGCGCTCGTCGACGCACCGAACCGCGCCGATTTTGCCTTCGGCGAAC
CGCGGCGGCTGACCTATGCCGAGTTGGCGGCGGAGATCGACCGGCTCGCGGGCGCGCTCGTCGCCGCGGGGATCGGCAAG
GACGATGTGCTGCTGGTTCAGCTTCCCAACATCGGCGAATTTGTCGCGCTCTATTTCGCGGCGGCCAAGATCGGTGCGAT
CGTCAGCCCGGCGGCGGTGCAGTATCGCAGCCACGAGCTGACGGGCATGATCGGCGTCGTCGAACCAAAAGCGTTCGTCT
GCGCCACGCGGGTCAAGGGCTGCGACCATGTCGGCGTCGCAGCGCCGTTGCTCGAAGGGATCGCGCTGATGACCTTCGGC
CCCAGCCCGCCCGCGGACGCACTGGACCTGTCGACCGCCCAAGGCGACGCCACCGCGCTTGCCGCGCATGTCGCGGCAAA
CCCGGTTGATGCCGACGATATCTTCACCATCTGCTGGACCTCGGGCACCACCGGCGTGCCAAAGGGCGTGCCGCGCAGCC
ACAACCACTGGATAGCGGTTGCCGCCGCGGGCTACGAGGCGATGAAGGTCGAGCCGGGCGATGTGCTGCTCAACCCCTTC
CCGCTCATCAACATGGCGAGCATCGGCGGCATCACCATGTGCTGGCTGACCAGCGCGGGGACGATGGTGCTGCACCATCC
GTTCGATCCCGGCGTGTACCTCAAGCAGATTGCGACCGAGCGGCCCAGCCTGACGATCGCGCCGCCCGCGGTGCTCAACA
TGCTGCTGCAGAATGAGGCTTTGCTCGCCTCGGTGGACCTGTCGAGCCTGCGCGTCATTGCCTCGGGCTCGGCACCGCTC
GCCCCCGCGATGGTGCGCGGTTTCCAGGAAAAGCTCGGCATCATCATCGTCAACGTCTTTGGCTCCAACGAGGGTATGAG
CTTCATCACCGGCGAGGGCGACATGCCCGACCCCGACAAGCGCGCGAGCCTTTTCCCGCGCCGCGGTCAATATCCGCCGC
CCTATGGCACCGGCCGCGCGCCCAACATCGAAAGCCGCCTCGTCCCGCCCGGCGGCGGCGACCCGATCGAGATGGACGGC
ATGGCGGGCGAGTTGCAGATCCGCGGCCCCAGCCTGTTCGAAGGCTATCACAACGCCCCCGAACAGACCGCCGCCGCCTT
CACCGATGACGGCTGGTTCCGCACCGGCGACCTGTTCGAGATCGCCGAAGGCGGCGACTTCTACCGCTTCGTCGGACGCT
GCAAGGATCTCATCATTCGCGGCGGAGTGAATATCGCGCCCGAGGAGATCGACCAACTGCTCGGCGGCCATCCACTCCTG
GCCGAAGCGTGCGTCTTCTCGCTCCCCGACCCCACGATGGGCGAGCGTATCGGCCTTGCCTATGTCCCGCGCGGCGCGGA
CGATGTCAGCCTTGCCGACGTGACCGACTATCTCCGCGCAAAGGATCTTGCGGTGTTCAAGCTGCCCGAACGCCTGTTCC
GCTTCGACGCGCTGCCGCGCAACGTCACGGGCAAGGTGATGCGCAGCGAGGTGCGCGAAATGGCGCTTGCGCAGCTCTCC
CTGAACAACCCCTCCCCTTCAGGGGAGGGGCAGTGA

Upstream 100 bases:

>100_bases
CGGATCATCCTGCCCGAGCGCATCTGTTCGGGCACGCACAGCGTGTGGGCGAGCGGCAATGATATCGGCATGGGCGAAAA
CAGCGTGCTGGCCGCCTGAG

Downstream 100 bases:

>100_bases
GACCTGGTCCGGCGCCAGCCGGGACTTTAGTCGAACGGGGCGGGGGCCATCGGCCTTGTGCAAGGTTGCGAGCCCCCACC
CCAACCCCTCCCCTGAAGGG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MIVAGEDKRRRYRQSGWWGDQSFADLFAANAAAHPDRLALVDAPNRADFAFGEPRRLTYAELAAEIDRLAGALVAAGIGK
DDVLLVQLPNIGEFVALYFAAAKIGAIVSPAAVQYRSHELTGMIGVVEPKAFVCATRVKGCDHVGVAAPLLEGIALMTFG
PSPPADALDLSTAQGDATALAAHVAANPVDADDIFTICWTSGTTGVPKGVPRSHNHWIAVAAAGYEAMKVEPGDVLLNPF
PLINMASIGGITMCWLTSAGTMVLHHPFDPGVYLKQIATERPSLTIAPPAVLNMLLQNEALLASVDLSSLRVIASGSAPL
APAMVRGFQEKLGIIIVNVFGSNEGMSFITGEGDMPDPDKRASLFPRRGQYPPPYGTGRAPNIESRLVPPGGGDPIEMDG
MAGELQIRGPSLFEGYHNAPEQTAAAFTDDGWFRTGDLFEIAEGGDFYRFVGRCKDLIIRGGVNIAPEEIDQLLGGHPLL
AEACVFSLPDPTMGERIGLAYVPRGADDVSLADVTDYLRAKDLAVFKLPERLFRFDALPRNVTGKVMRSEVREMALAQLS
LNNPSPSGEGQ

Sequences:

>Translated_571_residues
MIVAGEDKRRRYRQSGWWGDQSFADLFAANAAAHPDRLALVDAPNRADFAFGEPRRLTYAELAAEIDRLAGALVAAGIGK
DDVLLVQLPNIGEFVALYFAAAKIGAIVSPAAVQYRSHELTGMIGVVEPKAFVCATRVKGCDHVGVAAPLLEGIALMTFG
PSPPADALDLSTAQGDATALAAHVAANPVDADDIFTICWTSGTTGVPKGVPRSHNHWIAVAAAGYEAMKVEPGDVLLNPF
PLINMASIGGITMCWLTSAGTMVLHHPFDPGVYLKQIATERPSLTIAPPAVLNMLLQNEALLASVDLSSLRVIASGSAPL
APAMVRGFQEKLGIIIVNVFGSNEGMSFITGEGDMPDPDKRASLFPRRGQYPPPYGTGRAPNIESRLVPPGGGDPIEMDG
MAGELQIRGPSLFEGYHNAPEQTAAAFTDDGWFRTGDLFEIAEGGDFYRFVGRCKDLIIRGGVNIAPEEIDQLLGGHPLL
AEACVFSLPDPTMGERIGLAYVPRGADDVSLADVTDYLRAKDLAVFKLPERLFRFDALPRNVTGKVMRSEVREMALAQLS
LNNPSPSGEGQ
>Mature_571_residues
MIVAGEDKRRRYRQSGWWGDQSFADLFAANAAAHPDRLALVDAPNRADFAFGEPRRLTYAELAAEIDRLAGALVAAGIGK
DDVLLVQLPNIGEFVALYFAAAKIGAIVSPAAVQYRSHELTGMIGVVEPKAFVCATRVKGCDHVGVAAPLLEGIALMTFG
PSPPADALDLSTAQGDATALAAHVAANPVDADDIFTICWTSGTTGVPKGVPRSHNHWIAVAAAGYEAMKVEPGDVLLNPF
PLINMASIGGITMCWLTSAGTMVLHHPFDPGVYLKQIATERPSLTIAPPAVLNMLLQNEALLASVDLSSLRVIASGSAPL
APAMVRGFQEKLGIIIVNVFGSNEGMSFITGEGDMPDPDKRASLFPRRGQYPPPYGTGRAPNIESRLVPPGGGDPIEMDG
MAGELQIRGPSLFEGYHNAPEQTAAAFTDDGWFRTGDLFEIAEGGDFYRFVGRCKDLIIRGGVNIAPEEIDQLLGGHPLL
AEACVFSLPDPTMGERIGLAYVPRGADDVSLADVTDYLRAKDLAVFKLPERLFRFDALPRNVTGKVMRSEVREMALAQLS
LNNPSPSGEGQ

Specific function: Catalyzes the esterification, concomitant with transport, of exogenous fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. It has a low activity on medium or long chain fatty acids and is ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Membrane; Peripheral membrane protein. Note=Partially membrane-associated [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=573, Percent_Identity=27.3996509598604, Blast_Score=170, Evalue=4e-42,
Organism=Homo sapiens, GI187761345, Length=551, Percent_Identity=26.3157894736842, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI187761343, Length=551, Percent_Identity=26.3157894736842, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI42544132, Length=517, Percent_Identity=23.9845261121857, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI157311624, Length=525, Percent_Identity=25.5238095238095, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI157311622, Length=525, Percent_Identity=25.5238095238095, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI122937307, Length=520, Percent_Identity=25.9615384615385, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI58082049, Length=527, Percent_Identity=25.6166982922201, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI38505220, Length=512, Percent_Identity=24.21875, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI115511026, Length=528, Percent_Identity=23.4848484848485, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI38524616, Length=524, Percent_Identity=24.618320610687, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI40807357, Length=547, Percent_Identity=23.400365630713, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI13375727, Length=567, Percent_Identity=23.1040564373898, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI145693145, Length=565, Percent_Identity=30.6194690265487, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1786810, Length=565, Percent_Identity=29.3805309734513, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI221142682, Length=511, Percent_Identity=26.2230919765166, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788107, Length=579, Percent_Identity=22.6252158894646, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1790505, Length=575, Percent_Identity=24.1739130434783, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32563687, Length=533, Percent_Identity=25.515947467167, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17558820, Length=502, Percent_Identity=24.9003984063745, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17559526, Length=542, Percent_Identity=26.9372693726937, Blast_Score=152, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI71994703, Length=583, Percent_Identity=25.9005145797599, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI71994694, Length=583, Percent_Identity=25.9005145797599, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI71994690, Length=583, Percent_Identity=25.9005145797599, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17531443, Length=556, Percent_Identity=25.7194244604317, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=559, Percent_Identity=25.7602862254025, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17557194, Length=566, Percent_Identity=23.8515901060071, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI115534440, Length=565, Percent_Identity=21.5929203539823, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17538037, Length=547, Percent_Identity=24.6800731261426, Blast_Score=104, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71996755, Length=544, Percent_Identity=22.6102941176471, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=594, Percent_Identity=22.8956228956229, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6319699, Length=535, Percent_Identity=24.8598130841122, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6323182, Length=575, Percent_Identity=24.5217391304348, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6323903, Length=86, Percent_Identity=39.5348837209302, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24581924, Length=363, Percent_Identity=29.7520661157025, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI18859661, Length=517, Percent_Identity=25.7253384912959, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI21355181, Length=539, Percent_Identity=25.2319109461967, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI281365686, Length=524, Percent_Identity=22.7099236641221, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21358303, Length=491, Percent_Identity=23.8289205702648, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648253, Length=531, Percent_Identity=21.4689265536723, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648255, Length=531, Percent_Identity=21.6572504708098, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648260, Length=549, Percent_Identity=23.1329690346084, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24667955, Length=569, Percent_Identity=23.0228471001757, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI62472339, Length=570, Percent_Identity=23.1578947368421, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24583465, Length=527, Percent_Identity=23.9089184060721, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24583463, Length=527, Percent_Identity=23.9089184060721, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281364798, Length=528, Percent_Identity=23.8636363636364, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550496, Length=570, Percent_Identity=23.3333333333333, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI45552801, Length=570, Percent_Identity=23.3333333333333, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI20130353, Length=550, Percent_Identity=23.4545454545455, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28573631, Length=550, Percent_Identity=23.4545454545455, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI20130357, Length=521, Percent_Identity=24.7600767754319, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21355225, Length=553, Percent_Identity=23.869801084991, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24667959, Length=408, Percent_Identity=23.2843137254902, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24653035, Length=378, Percent_Identity=21.957671957672, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21356947, Length=377, Percent_Identity=23.342175066313, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 60793; Mature: 60793

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVAGEDKRRRYRQSGWWGDQSFADLFAANAAAHPDRLALVDAPNRADFAFGEPRRLTYA
CEECCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCHHH
ELAAEIDRLAGALVAAGIGKDDVLLVQLPNIGEFVALYFAAAKIGAIVSPAAVQYRSHEL
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
TGMIGVVEPKAFVCATRVKGCDHVGVAAPLLEGIALMTFGPSPPADALDLSTAQGDATAL
EEEEEEECCCCEEEEHHHCCCCCCCHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCHHHH
AAHVAANPVDADDIFTICWTSGTTGVPKGVPRSHNHWIAVAAAGYEAMKVEPGDVLLNPF
HHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCEEECCC
PLINMASIGGITMCWLTSAGTMVLHHPFDPGVYLKQIATERPSLTIAPPAVLNMLLQNEA
CCEEECCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHCCCCCEEECCHHHHHHHHCCCC
LLASVDLSSLRVIASGSAPLAPAMVRGFQEKLGIIIVNVFGSNEGMSFITGEGDMPDPDK
EEEEECHHHEEEEECCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCCCCCCCH
RASLFPRRGQYPPPYGTGRAPNIESRLVPPGGGDPIEMDGMAGELQIRGPSLFEGYHNAP
HHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEECCCCCEEEECCCHHHHHHCCCC
EQTAAAFTDDGWFRTGDLFEIAEGGDFYRFVGRCKDLIIRGGVNIAPEEIDQLLGGHPLL
HHHHHEECCCCCEECCCEEEECCCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHCCCCHH
AEACVFSLPDPTMGERIGLAYVPRGADDVSLADVTDYLRAKDLAVFKLPERLFRFDALPR
HHHHHHCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHCCCC
NVTGKVMRSEVREMALAQLSLNNPSPSGEGQ
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MIVAGEDKRRRYRQSGWWGDQSFADLFAANAAAHPDRLALVDAPNRADFAFGEPRRLTYA
CEECCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCHHH
ELAAEIDRLAGALVAAGIGKDDVLLVQLPNIGEFVALYFAAAKIGAIVSPAAVQYRSHEL
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
TGMIGVVEPKAFVCATRVKGCDHVGVAAPLLEGIALMTFGPSPPADALDLSTAQGDATAL
EEEEEEECCCCEEEEHHHCCCCCCCHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCHHHH
AAHVAANPVDADDIFTICWTSGTTGVPKGVPRSHNHWIAVAAAGYEAMKVEPGDVLLNPF
HHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCEEECCC
PLINMASIGGITMCWLTSAGTMVLHHPFDPGVYLKQIATERPSLTIAPPAVLNMLLQNEA
CCEEECCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHCCCCCEEECCHHHHHHHHCCCC
LLASVDLSSLRVIASGSAPLAPAMVRGFQEKLGIIIVNVFGSNEGMSFITGEGDMPDPDK
EEEEECHHHEEEEECCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCCCCCCCH
RASLFPRRGQYPPPYGTGRAPNIESRLVPPGGGDPIEMDGMAGELQIRGPSLFEGYHNAP
HHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEECCCCCEEEECCCHHHHHHCCCC
EQTAAAFTDDGWFRTGDLFEIAEGGDFYRFVGRCKDLIIRGGVNIAPEEIDQLLGGHPLL
HHHHHEECCCCCEECCCEEEECCCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHCCCCHH
AEACVFSLPDPTMGERIGLAYVPRGADDVSLADVTDYLRAKDLAVFKLPERLFRFDALPR
HHHHHHCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHCCCC
NVTGKVMRSEVREMALAQLSLNNPSPSGEGQ
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 7984428; 12535077 [H]