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The map label for this gene is leuA [H]

Identifier: 103486463

GI number: 103486463

Start: 998579

End: 1000243

Strand: Reverse

Name: leuA [H]

Synonym: Sala_0974

Alternate gene names: 103486463

Gene position: 1000243-998579 (Counterclockwise)

Preceding gene: 103486464

Following gene: 103486459

Centisome position: 29.9

GC content: 65.41

Gene sequence:

>1665_bases
ATGACCATGCTCCGCGACCCCTCGGTCAAATATAGCGCCTTTCCGCAGGTGCCGCTCGCCAAACGCGAATGGCCCGCCCG
CACCATCACCCGCGCCCCCATCTGGCTCTCGACCGACCTGCGCGACGGCAACCAGTCGCTGATCGACCCGATGGATGCCG
AGAAAAAGACCCGTTTCTTCGACCTGCTGGTGAAGGCCGGGTTCAAGGAGATCGAGGTCGGCTTCCCCGCCAGCGGCGCG
ACCGATTTCGACTATATCCAGAACCTCGTCCGTTCGGGCCGCGTCCCCGACGATGTCACCCCGCAGGTGCTGACCCAGAG
CCGCGCCGACCTCATCCGTACCAGCTTCGACAGCCTCGCCGGCGCGAAAACCGCGATCGTTCACGTCTATAACGCGCTCG
CCCCCGCGTGGCGCAAGATCGTCTTCGGCATGGACAAGGATGACATCAAGGCGATCGCGATCGACGGCGCGAAGCAACTG
CGCGACAATGCCGCGCGGCTTCCCGATACCGACTGGCGGTTCCAGTACAGCCCCGAATGCTTTTCGACCACCGAACTCGA
TTTCAGCATCGAGGTGTGCGAGGCGGTGATGGAGATCCTGAAGCCCACTTCGGCGAACCCGATCATCCTCAACCTGCCCG
CGACGGTCGAGGCGGCGACGCCGAACATCTACGCCGACCAGATCGAATATTTCGCGAAGAACCTGCCGAACCGCGACGCC
GCGATCATCAGCCTGCACACCCACAACGACCGCGGCACCGGCGTCGCGGCGGCCGAACTCGGCCTGCTCGCGGGCGCCGA
CCGCGTCGAGGGCTGCCTGTTCGGCAATGGCGAGCGCACCGGCAACACCTGCCTCGTCACCATCGCGCTCAACATGTACA
CGCAGGGCGTCGATCCCGGCCTCGATTTCTCCAACATCGACGAGGTCATCGCGACGGTCGAGTATTGCAACAATCTGCCC
GTCCACCCGCGCCACCCCTATGGCGGCGCACTCGTCTACACCGCTTTTTCGGGCAGCCATCAGGACGCGATCAAAAAGGG
CTTCGCCGCGCGCGAACGCCAGAATGACGAACGCTGGGAAGTCCCCTATCTGCCCATCGACCCCGCCGACCTGGGGCGCA
GCTATGAAGCGGTGATCCGCGTCAACAGCCAGTCGGGCAAGGGCGGCGTCGCCTGGGTGCTCGAACAGGACAAGGGGCTG
AAACTGCCCAAGAAGATGCAGGCGAGCTTCAGCCACATCGTCCAGGCGCTCGCCGACGAAACCAGCCGCGAACTCGGCGC
CGAGGACATCTGGCAGGCGTTCGAGCGCGCCTATCTGACCACCGACGCCAAGCGCTTCCAGCTGGTCGACTGGTCGGAGA
GCCATGCGGGCACCGACCGCATCTTCGCGGGCAAGCTCACCATCGACGGCATAGAGCGCAGCGTCAGCGGTCGCGGCAAC
GGCCTGATGTCGAGCGTCATCGCCGCGCTCGCCGAATCGGGCGGCCCGGTGATGGACATCGTCGATTACAGCGAACATGC
GATCGGCCAGGGCAGCAATGTGCAGGCCGCCGCCTATGTCGAATGCCGCACCGCCGACGGCAAAAGCCTGTTCGGCTGCG
GTCTCGACACCGACGTCGCGACGGCGAGCGTGCGCGCGATCCTCAGCGCGGCGAACGGCGCCTGA

Upstream 100 bases:

>100_bases
ACGCCCTTGGGCGTGACACTGGCTGCGCGTTCGTCGCGGCCACCCGCTCAAGGGTCCGATCGACACCGACACAGCCCCCT
GAAGAAGAAGTTTTCCTGCC

Downstream 100 bases:

>100_bases
TCATCCTCGCTGTCGCGCAGCAAGGGAGGTGGGGGTCAGACGCCTACGTCCTGGGAAGCAGCACCGCGTCGATCACATGG
ACGACGCCGTTCGCCTGCCC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MTMLRDPSVKYSAFPQVPLAKREWPARTITRAPIWLSTDLRDGNQSLIDPMDAEKKTRFFDLLVKAGFKEIEVGFPASGA
TDFDYIQNLVRSGRVPDDVTPQVLTQSRADLIRTSFDSLAGAKTAIVHVYNALAPAWRKIVFGMDKDDIKAIAIDGAKQL
RDNAARLPDTDWRFQYSPECFSTTELDFSIEVCEAVMEILKPTSANPIILNLPATVEAATPNIYADQIEYFAKNLPNRDA
AIISLHTHNDRGTGVAAAELGLLAGADRVEGCLFGNGERTGNTCLVTIALNMYTQGVDPGLDFSNIDEVIATVEYCNNLP
VHPRHPYGGALVYTAFSGSHQDAIKKGFAARERQNDERWEVPYLPIDPADLGRSYEAVIRVNSQSGKGGVAWVLEQDKGL
KLPKKMQASFSHIVQALADETSRELGAEDIWQAFERAYLTTDAKRFQLVDWSESHAGTDRIFAGKLTIDGIERSVSGRGN
GLMSSVIAALAESGGPVMDIVDYSEHAIGQGSNVQAAAYVECRTADGKSLFGCGLDTDVATASVRAILSAANGA

Sequences:

>Translated_554_residues
MTMLRDPSVKYSAFPQVPLAKREWPARTITRAPIWLSTDLRDGNQSLIDPMDAEKKTRFFDLLVKAGFKEIEVGFPASGA
TDFDYIQNLVRSGRVPDDVTPQVLTQSRADLIRTSFDSLAGAKTAIVHVYNALAPAWRKIVFGMDKDDIKAIAIDGAKQL
RDNAARLPDTDWRFQYSPECFSTTELDFSIEVCEAVMEILKPTSANPIILNLPATVEAATPNIYADQIEYFAKNLPNRDA
AIISLHTHNDRGTGVAAAELGLLAGADRVEGCLFGNGERTGNTCLVTIALNMYTQGVDPGLDFSNIDEVIATVEYCNNLP
VHPRHPYGGALVYTAFSGSHQDAIKKGFAARERQNDERWEVPYLPIDPADLGRSYEAVIRVNSQSGKGGVAWVLEQDKGL
KLPKKMQASFSHIVQALADETSRELGAEDIWQAFERAYLTTDAKRFQLVDWSESHAGTDRIFAGKLTIDGIERSVSGRGN
GLMSSVIAALAESGGPVMDIVDYSEHAIGQGSNVQAAAYVECRTADGKSLFGCGLDTDVATASVRAILSAANGA
>Mature_553_residues
TMLRDPSVKYSAFPQVPLAKREWPARTITRAPIWLSTDLRDGNQSLIDPMDAEKKTRFFDLLVKAGFKEIEVGFPASGAT
DFDYIQNLVRSGRVPDDVTPQVLTQSRADLIRTSFDSLAGAKTAIVHVYNALAPAWRKIVFGMDKDDIKAIAIDGAKQLR
DNAARLPDTDWRFQYSPECFSTTELDFSIEVCEAVMEILKPTSANPIILNLPATVEAATPNIYADQIEYFAKNLPNRDAA
IISLHTHNDRGTGVAAAELGLLAGADRVEGCLFGNGERTGNTCLVTIALNMYTQGVDPGLDFSNIDEVIATVEYCNNLPV
HPRHPYGGALVYTAFSGSHQDAIKKGFAARERQNDERWEVPYLPIDPADLGRSYEAVIRVNSQSGKGGVAWVLEQDKGLK
LPKKMQASFSHIVQALADETSRELGAEDIWQAFERAYLTTDAKRFQLVDWSESHAGTDRIFAGKLTIDGIERSVSGRGNG
LMSSVIAALAESGGPVMDIVDYSEHAIGQGSNVQAAAYVECRTADGKSLFGCGLDTDVATASVRAILSAANGA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=547, Percent_Identity=25.0457038391225, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324225, Length=565, Percent_Identity=50.7964601769912, Blast_Score=571, Evalue=1e-163,
Organism=Saccharomyces cerevisiae, GI6324682, Length=566, Percent_Identity=48.4098939929329, Blast_Score=556, Evalue=1e-159,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 60243; Mature: 60112

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLRDPSVKYSAFPQVPLAKREWPARTITRAPIWLSTDLRDGNQSLIDPMDAEKKTRFF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCEEEEECCCCCCHHHCCCCCCHHHHHHH
DLLVKAGFKEIEVGFPASGATDFDYIQNLVRSGRVPDDVTPQVLTQSRADLIRTSFDSLA
HHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GAKTAIVHVYNALAPAWRKIVFGMDKDDIKAIAIDGAKQLRDNAARLPDTDWRFQYSPEC
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCEEECCCC
FSTTELDFSIEVCEAVMEILKPTSANPIILNLPATVEAATPNIYADQIEYFAKNLPNRDA
CCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCEEECCCCCHHHHHHHHHHHCCCCCCE
AIISLHTHNDRGTGVAAAELGLLAGADRVEGCLFGNGERTGNTCLVTIALNMYTQGVDPG
EEEEEEECCCCCCCCHHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCC
LDFSNIDEVIATVEYCNNLPVHPRHPYGGALVYTAFSGSHQDAIKKGFAARERQNDERWE
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCC
VPYLPIDPADLGRSYEAVIRVNSQSGKGGVAWVLEQDKGLKLPKKMQASFSHIVQALADE
CCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
TSRELGAEDIWQAFERAYLTTDAKRFQLVDWSESHAGTDRIFAGKLTIDGIERSVSGRGN
HHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEEECCHHHHCCCCCC
GLMSSVIAALAESGGPVMDIVDYSEHAIGQGSNVQAAAYVECRTADGKSLFGCGLDTDVA
HHHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCCEEEEEEEEECCCCCEEEECCCCCHHH
TASVRAILSAANGA
HHHHHHHHHHCCCC
>Mature Secondary Structure 
TMLRDPSVKYSAFPQVPLAKREWPARTITRAPIWLSTDLRDGNQSLIDPMDAEKKTRFF
CCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCEEEEECCCCCCHHHCCCCCCHHHHHHH
DLLVKAGFKEIEVGFPASGATDFDYIQNLVRSGRVPDDVTPQVLTQSRADLIRTSFDSLA
HHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GAKTAIVHVYNALAPAWRKIVFGMDKDDIKAIAIDGAKQLRDNAARLPDTDWRFQYSPEC
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCEEECCCC
FSTTELDFSIEVCEAVMEILKPTSANPIILNLPATVEAATPNIYADQIEYFAKNLPNRDA
CCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCEEECCCCCHHHHHHHHHHHCCCCCCE
AIISLHTHNDRGTGVAAAELGLLAGADRVEGCLFGNGERTGNTCLVTIALNMYTQGVDPG
EEEEEEECCCCCCCCHHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCC
LDFSNIDEVIATVEYCNNLPVHPRHPYGGALVYTAFSGSHQDAIKKGFAARERQNDERWE
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCC
VPYLPIDPADLGRSYEAVIRVNSQSGKGGVAWVLEQDKGLKLPKKMQASFSHIVQALADE
CCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
TSRELGAEDIWQAFERAYLTTDAKRFQLVDWSESHAGTDRIFAGKLTIDGIERSVSGRGN
HHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEEECCHHHHCCCCCC
GLMSSVIAALAESGGPVMDIVDYSEHAIGQGSNVQAAAYVECRTADGKSLFGCGLDTDVA
HHHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCCEEEEEEEEECCCCCEEEECCCCCHHH
TASVRAILSAANGA
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA