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The map label for this gene is zraR [H]

Identifier: 103486459

GI number: 103486459

Start: 994533

End: 995996

Strand: Reverse

Name: zraR [H]

Synonym: Sala_0969

Alternate gene names: 103486459

Gene position: 995996-994533 (Counterclockwise)

Preceding gene: 103486463

Following gene: 103486458

Centisome position: 29.77

GC content: 67.96

Gene sequence:

>1464_bases
ATGCACAGCGCTCCAGGGGACAGATTCGAAACCATGGCTAGCACGCGCGACACGCGAATGGTGATGATCGTCGACAGCGA
GCCTGCACAGCAGCGCTTCCTGTCGGCGCTCGTGTCGCGTGGCGGCTGGCGCAGCGTCGTGGCCGCCGACACCGATACCG
CGCTCGCCAAGCTCGGCACGCAGGAGGGGATGGCCCTCGACGCCGTTCTCATCGATCAGGGCGCACCTGGCATGGACATT
GCCCAATTCGTGATCGAACTCCGCCGCTGGCGCCCCGCACTGCCACTCGTCGTCATCACCATGCGCAACGGGGTCGAGGT
TGCGGTGGCGGCGATGCGCGCCGGGGCGAGCGACTTCGTCCAGAAACCCATCGCGCCCGACCGGCTGCTCGAAGCGCTCG
ACCGGGTGATGGCCGACAGCGGCCCCCAAGGCGAGCTGCGTCCGCTAACCGAAAAGCTGCGCGCGCCGCTCGCGTTCGAG
GAAATCATCGGGTCCAGCCCCAATTTCCGCACGGCGCTCGCCATCGCAGCCAAGGCCGCACGCGCGCGAGTTCCGGTGAT
GATCAACGGCAAACCCGGCACCGGCAAGGAGGTCTTTGCCCGCGCGATCCATGGCGCGAGTCCGCGCGCACGCGGCCCGC
TCGTCATGGTCGATTGTTCGGCGGTTTCGCCCGGCCTGATCGGATCGGGGCTGTTCGGCCACGAACGCGGCGCCTTTCCC
GGTGCCTTCGACCGCCAGATTGGTCGTCTTGTCCAAGCCGACGGCGGCAGCATCATCATCGACCATGTCGAGTGCATCCC
GCTCGACACCCAGGCAAAGCTCGTCGAGTTTCTGAACAACGGCGAGCTGCAGATGATCGGCGGGTCGATCCGCCAGACCG
TCGATGTGCGCATTATCGCGACGAGCGCCAGCCCGCTCGACAAGCTCATCGAGGCGGGTCATTTCCGCGAAGATCTGTTC
TACGCGCTGTCGAGCGCGCAGCTCACCCTGCCTTCGCTCAGCGAGCGCCGCGGCGACGTCGGCCCGCTCGCGCGCCACCT
GCTCGCGCGGATCGGCGGTCTCCCCGGCATGGGGTCGATCGGCATCACTGACGACGCGCTGCGCCTGCTTGCCGCCTATG
CCTGGCCGGGCAATGTCCGCCAGCTTCAGGATGTGCTGTTCCGCGCCGCAGTGGGCAGCACCACCGATGTCCTGACCGCC
GCCGATTTCCGTGCAATCGAAGCGACGCTCGCCGGCGGCACCGCCAGCGACGGCGAGGTGGCGATCAACGGCGAGGCGAT
CGGCGTCACGCTCTACCTGCCTGACGGCAATCTGCGTCCGCTCGAGGAGATCGAGGCCGACGTCATCCGCCTCGCGATCG
GCCACTATCGCGGCCGCATGAGCGAAGTCGCCCGGCGGCTGGGGATCGGGCGTTCGACGCTCTATCGCAAGCTCGGCGAC
CTCGGGATCGACACGGCGGCCTGA

Upstream 100 bases:

>100_bases
TAGCCGGTTTGGCCTGTCGGGAGCAGCAGGGCAAAAGGCGAACGGCCTTAATCGCCCTTTTACTCCTCACCGATAGGAAG
GGTGTTCACGACAGCAATGG

Downstream 100 bases:

>100_bases
TACGCTTGCGGATCGGTTTGATTGGGCTGCTCGATCGGGCTACTTGATATATAAGTTTGAACTTATATGATGTCCCGACA
GAAATCGGGAGAATCATCAT

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MHSAPGDRFETMASTRDTRMVMIVDSEPAQQRFLSALVSRGGWRSVVAADTDTALAKLGTQEGMALDAVLIDQGAPGMDI
AQFVIELRRWRPALPLVVITMRNGVEVAVAAMRAGASDFVQKPIAPDRLLEALDRVMADSGPQGELRPLTEKLRAPLAFE
EIIGSSPNFRTALAIAAKAARARVPVMINGKPGTGKEVFARAIHGASPRARGPLVMVDCSAVSPGLIGSGLFGHERGAFP
GAFDRQIGRLVQADGGSIIIDHVECIPLDTQAKLVEFLNNGELQMIGGSIRQTVDVRIIATSASPLDKLIEAGHFREDLF
YALSSAQLTLPSLSERRGDVGPLARHLLARIGGLPGMGSIGITDDALRLLAAYAWPGNVRQLQDVLFRAAVGSTTDVLTA
ADFRAIEATLAGGTASDGEVAINGEAIGVTLYLPDGNLRPLEEIEADVIRLAIGHYRGRMSEVARRLGIGRSTLYRKLGD
LGIDTAA

Sequences:

>Translated_487_residues
MHSAPGDRFETMASTRDTRMVMIVDSEPAQQRFLSALVSRGGWRSVVAADTDTALAKLGTQEGMALDAVLIDQGAPGMDI
AQFVIELRRWRPALPLVVITMRNGVEVAVAAMRAGASDFVQKPIAPDRLLEALDRVMADSGPQGELRPLTEKLRAPLAFE
EIIGSSPNFRTALAIAAKAARARVPVMINGKPGTGKEVFARAIHGASPRARGPLVMVDCSAVSPGLIGSGLFGHERGAFP
GAFDRQIGRLVQADGGSIIIDHVECIPLDTQAKLVEFLNNGELQMIGGSIRQTVDVRIIATSASPLDKLIEAGHFREDLF
YALSSAQLTLPSLSERRGDVGPLARHLLARIGGLPGMGSIGITDDALRLLAAYAWPGNVRQLQDVLFRAAVGSTTDVLTA
ADFRAIEATLAGGTASDGEVAINGEAIGVTLYLPDGNLRPLEEIEADVIRLAIGHYRGRMSEVARRLGIGRSTLYRKLGD
LGIDTAA
>Mature_487_residues
MHSAPGDRFETMASTRDTRMVMIVDSEPAQQRFLSALVSRGGWRSVVAADTDTALAKLGTQEGMALDAVLIDQGAPGMDI
AQFVIELRRWRPALPLVVITMRNGVEVAVAAMRAGASDFVQKPIAPDRLLEALDRVMADSGPQGELRPLTEKLRAPLAFE
EIIGSSPNFRTALAIAAKAARARVPVMINGKPGTGKEVFARAIHGASPRARGPLVMVDCSAVSPGLIGSGLFGHERGAFP
GAFDRQIGRLVQADGGSIIIDHVECIPLDTQAKLVEFLNNGELQMIGGSIRQTVDVRIIATSASPLDKLIEAGHFREDLF
YALSSAQLTLPSLSERRGDVGPLARHLLARIGGLPGMGSIGITDDALRLLAAYAWPGNVRQLQDVLFRAAVGSTTDVLTA
ADFRAIEATLAGGTASDGEVAINGEAIGVTLYLPDGNLRPLEEIEADVIRLAIGHYRGRMSEVARRLGIGRSTLYRKLGD
LGIDTAA

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=467, Percent_Identity=34.475374732334, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI1790299, Length=425, Percent_Identity=34.8235294117647, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1790437, Length=468, Percent_Identity=34.1880341880342, Blast_Score=241, Evalue=9e-65,
Organism=Escherichia coli, GI1788905, Length=464, Percent_Identity=30.6034482758621, Blast_Score=214, Evalue=9e-57,
Organism=Escherichia coli, GI87082117, Length=249, Percent_Identity=39.7590361445783, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1787583, Length=332, Percent_Identity=32.8313253012048, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1789087, Length=344, Percent_Identity=34.8837209302326, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=34.8909657320872, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI87082152, Length=335, Percent_Identity=33.7313432835821, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI87081872, Length=239, Percent_Identity=35.5648535564854, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI87081858, Length=348, Percent_Identity=28.735632183908, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1786524, Length=254, Percent_Identity=33.8582677165354, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789828, Length=248, Percent_Identity=31.0483870967742, Blast_Score=111, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51727; Mature: 51727

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSAPGDRFETMASTRDTRMVMIVDSEPAQQRFLSALVSRGGWRSVVAADTDTALAKLGT
CCCCCCCHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHCCC
QEGMALDAVLIDQGAPGMDIAQFVIELRRWRPALPLVVITMRNGVEVAVAAMRAGASDFV
CCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCHHHHH
QKPIAPDRLLEALDRVMADSGPQGELRPLTEKLRAPLAFEEIIGSSPNFRTALAIAAKAA
HCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHH
RARVPVMINGKPGTGKEVFARAIHGASPRARGPLVMVDCSAVSPGLIGSGLFGHERGAFP
HCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
GAFDRQIGRLVQADGGSIIIDHVECIPLDTQAKLVEFLNNGELQMIGGSIRQTVDVRIIA
CHHHHHHHHHHCCCCCEEEEECEEEECCCHHHHHHHHHCCCCEEEECCCHHCEEEEEEEE
TSASPLDKLIEAGHFREDLFYALSSAQLTLPSLSERRGDVGPLARHLLARIGGLPGMGSI
ECCCHHHHHHHCCCHHHHHHHHHHCCCEECCCHHHHCCCCCHHHHHHHHHHCCCCCCCCC
GITDDALRLLAAYAWPGNVRQLQDVLFRAAVGSTTDVLTAADFRAIEATLAGGTASDGEV
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCCCCCE
AINGEAIGVTLYLPDGNLRPLEEIEADVIRLAIGHYRGRMSEVARRLGIGRSTLYRKLGD
EECCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
LGIDTAA
CCCCCCC
>Mature Secondary Structure
MHSAPGDRFETMASTRDTRMVMIVDSEPAQQRFLSALVSRGGWRSVVAADTDTALAKLGT
CCCCCCCHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHCCC
QEGMALDAVLIDQGAPGMDIAQFVIELRRWRPALPLVVITMRNGVEVAVAAMRAGASDFV
CCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCHHHHH
QKPIAPDRLLEALDRVMADSGPQGELRPLTEKLRAPLAFEEIIGSSPNFRTALAIAAKAA
HCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHH
RARVPVMINGKPGTGKEVFARAIHGASPRARGPLVMVDCSAVSPGLIGSGLFGHERGAFP
HCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
GAFDRQIGRLVQADGGSIIIDHVECIPLDTQAKLVEFLNNGELQMIGGSIRQTVDVRIIA
CHHHHHHHHHHCCCCCEEEEECEEEECCCHHHHHHHHHCCCCEEEECCCHHCEEEEEEEE
TSASPLDKLIEAGHFREDLFYALSSAQLTLPSLSERRGDVGPLARHLLARIGGLPGMGSI
ECCCHHHHHHHCCCHHHHHHHHHHCCCEECCCHHHHCCCCCHHHHHHHHHHCCCCCCCCC
GITDDALRLLAAYAWPGNVRQLQDVLFRAAVGSTTDVLTAADFRAIEATLAGGTASDGEV
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCCCCCE
AINGEAIGVTLYLPDGNLRPLEEIEADVIRLAIGHYRGRMSEVARRLGIGRSTLYRKLGD
EECCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
LGIDTAA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11243806 [H]