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The map label for this gene is budC [H]

Identifier: 103486373

GI number: 103486373

Start: 888982

End: 889776

Strand: Direct

Name: budC [H]

Synonym: Sala_0883

Alternate gene names: 103486373

Gene position: 888982-889776 (Clockwise)

Preceding gene: 103486372

Following gene: 103486374

Centisome position: 26.58

GC content: 62.77

Gene sequence:

>795_bases
ATGGACCTTGGCATCGCGGGCAAGGTGGCGCTCGTCTTTGGCGGCTCGAAGGGAATCGGCCTCGGTTGCGCGCATGAATT
TGCGCGGGAAGGCTGCCGCGTCGTGATTGCCGCCCGCACCCAGGCGACGATCGACGCCGCCGTCGCCGAAGTGCAGGCGA
CGGGCGGTCAGGCGATCGGAGTGTCGGCAGATTGCACGACGAAAGATGGGATAGGGCGGGCGGTGCAGGCGGCGACCGAT
GCGTTCGCCCCGCCCGACATCCTGATCTTCAACGTCGATTCCGGTCCGAAGGGAAGTTTTCTCGAAGTCGATGACGATAT
CTTTGCCGCCGCCAATACCAACAATGTCATGGCGTTCCGCTGGGCGGTGCAGGCGGTGTATCCGTATATGCAGTCGCGCC
AATGGGGACGCATCCTGACCATCGGCACCAACTCGGTAAAAGCGCCCCATCGCAAGCTCCCGCGCGCCGCCCAGAACACA
TATCGCGTCGGTGCGCTGGCGCTGTCGAAAACCCTGTCGGCCGAACTCGGCCCCTTTGGCATCACCGTGAACACGCTGGG
AACCGGTGCAATCGCGACGCCGCAGTTCAAGGAGGTATTCACCAGGATCGCCGAAAAGCATGGCCAAAGCTACGACGAAC
ATATCGCCGAACGAACGAGCCAATACCCGATCCCGCGCATGGGGACGCCGGAGGATATGGCGGCGGCAGCCGCTTTTCTG
TGTTCCGACCGGGCGGGTTTCATCACCGGCCAGGTGCTGGTCGTCGATGGCGGCAATCTGGAAGTTCTGCAATAA

Upstream 100 bases:

>100_bases
CGACGATCAAGATGGACGGGGGCTCGACCGTCGCGGCATCGCCGGGGCGGAACGACATTCTTCAGGAGCGGCTGAAACGT
CAGCAGGGAAAGGCCCCCTG

Downstream 100 bases:

>100_bases
CCGAAAAATGAAGGAAAAAGGAAGATGAAGCTCGATAACAGCATCGCCGCCGTCGTGACCGGGGGAGCGTCGGGACTTGG
CCGGGCAAGCGCCGAGGCGC

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MDLGIAGKVALVFGGSKGIGLGCAHEFAREGCRVVIAARTQATIDAAVAEVQATGGQAIGVSADCTTKDGIGRAVQAATD
AFAPPDILIFNVDSGPKGSFLEVDDDIFAAANTNNVMAFRWAVQAVYPYMQSRQWGRILTIGTNSVKAPHRKLPRAAQNT
YRVGALALSKTLSAELGPFGITVNTLGTGAIATPQFKEVFTRIAEKHGQSYDEHIAERTSQYPIPRMGTPEDMAAAAAFL
CSDRAGFITGQVLVVDGGNLEVLQ

Sequences:

>Translated_264_residues
MDLGIAGKVALVFGGSKGIGLGCAHEFAREGCRVVIAARTQATIDAAVAEVQATGGQAIGVSADCTTKDGIGRAVQAATD
AFAPPDILIFNVDSGPKGSFLEVDDDIFAAANTNNVMAFRWAVQAVYPYMQSRQWGRILTIGTNSVKAPHRKLPRAAQNT
YRVGALALSKTLSAELGPFGITVNTLGTGAIATPQFKEVFTRIAEKHGQSYDEHIAERTSQYPIPRMGTPEDMAAAAAFL
CSDRAGFITGQVLVVDGGNLEVLQ
>Mature_264_residues
MDLGIAGKVALVFGGSKGIGLGCAHEFAREGCRVVIAARTQATIDAAVAEVQATGGQAIGVSADCTTKDGIGRAVQAATD
AFAPPDILIFNVDSGPKGSFLEVDDDIFAAANTNNVMAFRWAVQAVYPYMQSRQWGRILTIGTNSVKAPHRKLPRAAQNT
YRVGALALSKTLSAELGPFGITVNTLGTGAIATPQFKEVFTRIAEKHGQSYDEHIAERTSQYPIPRMGTPEDMAAAAAFL
CSDRAGFITGQVLVVDGGNLEVLQ

Specific function: Catalyzes the reversible reduction of (S)-acetoin to 2,3-butanediol in the presence of NADH [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=255, Percent_Identity=32.9411764705882, Blast_Score=117, Evalue=9e-27,
Organism=Homo sapiens, GI126723750, Length=259, Percent_Identity=30.8880308880309, Blast_Score=101, Evalue=5e-22,
Organism=Homo sapiens, GI5031737, Length=254, Percent_Identity=29.5275590551181, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI19923817, Length=264, Percent_Identity=28.030303030303, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI7705925, Length=261, Percent_Identity=29.5019157088123, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI33667109, Length=197, Percent_Identity=29.4416243654822, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI126723191, Length=183, Percent_Identity=29.5081967213115, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI40254992, Length=251, Percent_Identity=24.7011952191235, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1787905, Length=265, Percent_Identity=32.0754716981132, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082100, Length=256, Percent_Identity=28.515625, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787335, Length=256, Percent_Identity=30.859375, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790717, Length=260, Percent_Identity=26.9230769230769, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789208, Length=257, Percent_Identity=24.9027237354086, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1789057, Length=259, Percent_Identity=26.2548262548263, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI2367365, Length=255, Percent_Identity=29.8039215686275, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788459, Length=255, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17560676, Length=256, Percent_Identity=28.90625, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17561402, Length=255, Percent_Identity=27.0588235294118, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17538480, Length=278, Percent_Identity=28.0575539568345, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17562910, Length=268, Percent_Identity=27.9850746268657, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17544670, Length=271, Percent_Identity=25.0922509225092, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560332, Length=270, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17568967, Length=198, Percent_Identity=29.7979797979798, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17559104, Length=269, Percent_Identity=28.6245353159851, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17562904, Length=276, Percent_Identity=25.3623188405797, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17551412, Length=276, Percent_Identity=26.0869565217391, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994600, Length=257, Percent_Identity=25.6809338521401, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=258, Percent_Identity=26.7441860465116, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21355319, Length=255, Percent_Identity=27.843137254902, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI23397609, Length=260, Percent_Identity=27.6923076923077, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24639444, Length=265, Percent_Identity=26.4150943396226, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357041, Length=254, Percent_Identity=27.1653543307087, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28571526, Length=253, Percent_Identity=26.8774703557312, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24644339, Length=263, Percent_Identity=25.8555133079848, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014007
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.304 [H]

Molecular weight: Translated: 27744; Mature: 27744

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLGIAGKVALVFGGSKGIGLGCAHEFAREGCRVVIAARTQATIDAAVAEVQATGGQAIG
CCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCEEE
VSADCTTKDGIGRAVQAATDAFAPPDILIFNVDSGPKGSFLEVDDDIFAAANTNNVMAFR
CCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECCCEEEECCCCCEEEHH
WAVQAVYPYMQSRQWGRILTIGTNSVKAPHRKLPRAAQNTYRVGALALSKTLSAELGPFG
HHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCHHHHCCHHHHHHHHHHHHHCCCCCCE
ITVNTLGTGAIATPQFKEVFTRIAEKHGQSYDEHIAERTSQYPIPRMGTPEDMAAAAAFL
EEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
CSDRAGFITGQVLVVDGGNLEVLQ
HCCCCCEEEEEEEEEECCCEEEEC
>Mature Secondary Structure
MDLGIAGKVALVFGGSKGIGLGCAHEFAREGCRVVIAARTQATIDAAVAEVQATGGQAIG
CCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCEEE
VSADCTTKDGIGRAVQAATDAFAPPDILIFNVDSGPKGSFLEVDDDIFAAANTNNVMAFR
CCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECCCEEEECCCCCEEEHH
WAVQAVYPYMQSRQWGRILTIGTNSVKAPHRKLPRAAQNTYRVGALALSKTLSAELGPFG
HHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCHHHHCCHHHHHHHHHHHHHCCCCCCE
ITVNTLGTGAIATPQFKEVFTRIAEKHGQSYDEHIAERTSQYPIPRMGTPEDMAAAAAFL
EEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
CSDRAGFITGQVLVVDGGNLEVLQ
HCCCCCEEEEEEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11173520 [H]