BacMap

Definition	Sphingopyxis alaskensis RB2256, complete genome.
Accession	NC_008048
Length	3,345,170

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The map label for this gene is fabG [C]

Identifier: 103486372

GI number: 103486372

Start: 888161

End: 888982

Strand: Direct

Name: fabG [C]

Synonym: Sala_0882

Alternate gene names: 103486372

Gene position: 888161-888982 (Clockwise)

Preceding gene: 103486371

Following gene: 103486373

Centisome position: 26.55

GC content: 67.76

Gene sequence:

>822_bases
ATGATGGAAGATTTTTCGGGCAAGGTGGTAATCGTCACCGGCGGCGGACGCGGCGTAGGGCGCGGTGTCGCAAAGGCTTT
CGCGGCGGCGGGCGCCAAGGTCATGCTCGGGGCGCGCACCATCTCGCATGCGGAGGACGTAAAGGCTGAGATTGAAGCGA
CGGGCGGTATCGCCGAATGTTTCGCCGCCGACATCGCGCGCCACGCCGATTGCCGCGCACTGGTCGATGCGGCGGTTGAG
GCGTTTGGCGGCGTCGACATCATCGTCCATGCCGCCGCCGACATTCCGCATGGCGGGGTTGGCGAGGTCGACGACGAGCG
GCTGGAGGCAGGCTTTGCCAGCATCGCCAAGGCCGCCTGGTGGCTGCTCGACGCCGCGCGGCCGCATCTGGCGAAGGCGC
GGGACGGCGGCCGCTTCATCGCGATCGGATCGGTCAACGGCACCTTCAATGTCGTCCCCAACATGACCGCTTACGGGATG
GCAAAGGCGGCGCTCGACGCCTTCATCCGTGCCGCGGCGGGCGATGTGGTCGGGGAGGGGATTACCGTCAACGCGATCAA
TCCCGGCCTGATCGCCAGCGACCGCGCCAAGGCGGTGCTCGGCGACGATGGGTTGGCGGCCTATGGCTCGACGGTGCCGG
TGGGCCGGGCCGGCACGCCCGAGGACATTGCCCACGGCTGCCTGTTCCTGGCTTCGGCGCGATCCGACTATATCACCGGC
ACGACGATCAAGATGGACGGGGGCTCGACCGTCGCGGCATCGCCGGGGCGGAACGACATTCTTCAGGAGCGGCTGAAACG
TCAGCAGGGAAAGGCCCCCTGA

Upstream 100 bases:

>100_bases
ATCAGGTGCCGACCCGCATCCAGATCGTCACCGACTTTCCGCGCACGCCGTCCATGAAGCCCTCGGCACCGGGCCTGCGC
GCCCTTTTTGCGGAGGATGA

Downstream 100 bases:

>100_bases
TGGACCTTGGCATCGCGGGCAAGGTGGCGCTCGTCTTTGGCGGCTCGAAGGGAATCGGCCTCGGTTGCGCGCATGAATTT
GCGCGGGAAGGCTGCCGCGT

Product: short-chain dehydrogenase/reductase SDR

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MMEDFSGKVVIVTGGGRGVGRGVAKAFAAAGAKVMLGARTISHAEDVKAEIEATGGIAECFAADIARHADCRALVDAAVE
AFGGVDIIVHAAADIPHGGVGEVDDERLEAGFASIAKAAWWLLDAARPHLAKARDGGRFIAIGSVNGTFNVVPNMTAYGM
AKAALDAFIRAAAGDVVGEGITVNAINPGLIASDRAKAVLGDDGLAAYGSTVPVGRAGTPEDIAHGCLFLASARSDYITG
TTIKMDGGSTVAASPGRNDILQERLKRQQGKAP

Sequences:

>Translated_273_residues
MMEDFSGKVVIVTGGGRGVGRGVAKAFAAAGAKVMLGARTISHAEDVKAEIEATGGIAECFAADIARHADCRALVDAAVE
AFGGVDIIVHAAADIPHGGVGEVDDERLEAGFASIAKAAWWLLDAARPHLAKARDGGRFIAIGSVNGTFNVVPNMTAYGM
AKAALDAFIRAAAGDVVGEGITVNAINPGLIASDRAKAVLGDDGLAAYGSTVPVGRAGTPEDIAHGCLFLASARSDYITG
TTIKMDGGSTVAASPGRNDILQERLKRQQGKAP
>Mature_273_residues
MMEDFSGKVVIVTGGGRGVGRGVAKAFAAAGAKVMLGARTISHAEDVKAEIEATGGIAECFAADIARHADCRALVDAAVE
AFGGVDIIVHAAADIPHGGVGEVDDERLEAGFASIAKAAWWLLDAARPHLAKARDGGRFIAIGSVNGTFNVVPNMTAYGM
AKAALDAFIRAAAGDVVGEGITVNAINPGLIASDRAKAVLGDDGLAAYGSTVPVGRAGTPEDIAHGCLFLASARSDYITG
TTIKMDGGSTVAASPGRNDILQERLKRQQGKAP

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=249, Percent_Identity=28.5140562248996, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI5031737, Length=248, Percent_Identity=27.8225806451613, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI40254992, Length=241, Percent_Identity=29.4605809128631, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI7705925, Length=250, Percent_Identity=30.4, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI66933014, Length=252, Percent_Identity=25.7936507936508, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI126723750, Length=252, Percent_Identity=25, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI19923817, Length=274, Percent_Identity=24.8175182481752, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI33667109, Length=267, Percent_Identity=26.2172284644195, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI20149619, Length=240, Percent_Identity=30.4166666666667, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI40254986, Length=214, Percent_Identity=26.6355140186916, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI15277342, Length=246, Percent_Identity=27.6422764227642, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787335, Length=245, Percent_Identity=34.2857142857143, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789378, Length=237, Percent_Identity=32.9113924050633, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082100, Length=260, Percent_Identity=29.2307692307692, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1789208, Length=245, Percent_Identity=30.6122448979592, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1787905, Length=250, Percent_Identity=32.4, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1786812, Length=266, Percent_Identity=29.3233082706767, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI2367365, Length=249, Percent_Identity=30.5220883534137, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790717, Length=249, Percent_Identity=24.0963855421687, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082160, Length=263, Percent_Identity=29.6577946768061, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI2367175, Length=247, Percent_Identity=26.3157894736842, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788459, Length=252, Percent_Identity=25.7936507936508, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193204405, Length=283, Percent_Identity=33.2155477031802, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17562906, Length=267, Percent_Identity=33.7078651685393, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17562908, Length=272, Percent_Identity=33.4558823529412, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17562990, Length=278, Percent_Identity=30.9352517985612, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17560332, Length=271, Percent_Identity=33.210332103321, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17531453, Length=282, Percent_Identity=30.8510638297872, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17562910, Length=280, Percent_Identity=31.0714285714286, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17559104, Length=267, Percent_Identity=32.2097378277154, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17551412, Length=272, Percent_Identity=33.4558823529412, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17560150, Length=274, Percent_Identity=30.6569343065693, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17538480, Length=275, Percent_Identity=33.8181818181818, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17538486, Length=269, Percent_Identity=32.3420074349442, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17562904, Length=277, Percent_Identity=28.8808664259928, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17565030, Length=285, Percent_Identity=30.8771929824561, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17544670, Length=289, Percent_Identity=28.719723183391, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17560220, Length=281, Percent_Identity=31.6725978647687, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI72000259, Length=267, Percent_Identity=29.2134831460674, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI71994600, Length=253, Percent_Identity=30.4347826086957, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17561402, Length=252, Percent_Identity=32.1428571428571, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71994604, Length=252, Percent_Identity=29.3650793650794, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17563726, Length=284, Percent_Identity=28.169014084507, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17560676, Length=246, Percent_Identity=28.8617886178862, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17555706, Length=248, Percent_Identity=28.6290322580645, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17536025, Length=270, Percent_Identity=30, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115534694, Length=246, Percent_Identity=23.9837398373984, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI25147288, Length=246, Percent_Identity=26.8292682926829, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17508651, Length=248, Percent_Identity=27.0161290322581, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI193207185, Length=160, Percent_Identity=31.875, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17564282, Length=142, Percent_Identity=28.8732394366197, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71982365, Length=207, Percent_Identity=25.6038647342995, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI212645366, Length=139, Percent_Identity=33.0935251798561, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17561272, Length=260, Percent_Identity=30.3846153846154, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17538482, Length=131, Percent_Identity=33.587786259542, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=262, Percent_Identity=23.6641221374046, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI23397609, Length=249, Percent_Identity=30.9236947791165, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21355319, Length=251, Percent_Identity=31.0756972111554, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24644339, Length=251, Percent_Identity=28.6852589641434, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21357041, Length=253, Percent_Identity=28.8537549407115, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28571526, Length=253, Percent_Identity=28.0632411067194, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24644337, Length=254, Percent_Identity=30.3149606299213, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24639444, Length=246, Percent_Identity=28.8617886178862, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21357673, Length=195, Percent_Identity=26.1538461538462, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 27707; Mature: 27707

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEDFSGKVVIVTGGGRGVGRGVAKAFAAAGAKVMLGARTISHAEDVKAEIEATGGIAEC
CCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCHHHH
FAADIARHADCRALVDAAVEAFGGVDIIVHAAADIPHGGVGEVDDERLEAGFASIAKAAW
HHHHHHHCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
WLLDAARPHLAKARDGGRFIAIGSVNGTFNVVPNMTAYGMAKAALDAFIRAAAGDVVGEG
HHHHHCCCHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCC
ITVNAINPGLIASDRAKAVLGDDGLAAYGSTVPVGRAGTPEDIAHGCLFLASARSDYITG
EEEECCCCCCEECCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCEEC
TTIKMDGGSTVAASPGRNDILQERLKRQQGKAP
EEEEECCCCEEECCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMEDFSGKVVIVTGGGRGVGRGVAKAFAAAGAKVMLGARTISHAEDVKAEIEATGGIAEC
CCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCHHHH
FAADIARHADCRALVDAAVEAFGGVDIIVHAAADIPHGGVGEVDDERLEAGFASIAKAAW
HHHHHHHCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
WLLDAARPHLAKARDGGRFIAIGSVNGTFNVVPNMTAYGMAKAALDAFIRAAAGDVVGEG
HHHHHCCCHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCC
ITVNAINPGLIASDRAKAVLGDDGLAAYGSTVPVGRAGTPEDIAHGCLFLASARSDYITG
EEEECCCCCCEECCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCEEC
TTIKMDGGSTVAASPGRNDILQERLKRQQGKAP
EEEEECCCCEEECCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]