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The map label for this gene is fadK [H]

Identifier: 103486359

GI number: 103486359

Start: 875269

End: 876780

Strand: Direct

Name: fadK [H]

Synonym: Sala_0869

Alternate gene names: 103486359

Gene position: 875269-876780 (Clockwise)

Preceding gene: 103486358

Following gene: 103486363

Centisome position: 26.17

GC content: 64.02

Gene sequence:

>1512_bases
ATGACGGCATCGTTCATCGATGCGATGATCGCGGCGGCGGCGCGTGCGCCGCAGGCGTCGCTGATCGTCGCATCGGACAT
GCGCCCGTGTGATACCACGCTCGGTGAGGTCACGGCGGCGGGGCGCCGCATGGGAAGCCGGATGACCAAGGCGGGAGTTC
GGCCTGGTGATGTGGTCGGCTGTATGCTCCCCAATTGGCGCGAATGGCTCGTCGTCGCGGTTGCCGCGGCACAGGCTGGC
GCGGTCATGCTGCCGATCGTCACCATCTATGGTGCAAAGGAACTGGCGTTTATCCTGCGTCAGTCGAAGGCGCGCTGGCT
TTTTACGCCCGACCATTTTCGCGGCACCGATTATGGGCGCCTTGTCGCCGAATGCGGCGAATTGCCGATGCTCGAACGGC
ATATCCGTGCCGGAGAGGATTTCGGTGCGCTGGAAAGCGACGGGGCGATGGCGGACCCTGTACCGTCTGATCCCGACGAA
CTCGCGCTGCTGGTATATACGTCGGGGACCACCGCCGATCCCAAGGGGGTGATGCACAGCGCGCGCACTTTGCTGGCCGA
GATGGCCTCGATGCGGGAAATGCGGCGTGAGAAGGACGAGGATATTGTCATCTCGCCCTGGCCTCCCGGCCATGTCGCGG
GCGCGCTGTCGATGTACCGCTTTCTTTGCCAGGGAACGCCGCTGGTGCTGATGGATCAGTGGGACGCAGCGCTCGCCGCC
GAGCTGATCGATCGCCACAAGCCGACCTCCTCGTCGGGAACGCCCTTTCACCTGTCGGGCTTGCTCGCGGCGGCCGACGT
CCATGGCCATGACCTCTCTTCGCTCCGCCAGTATCTGGTCGGTGCGGCGCCGGTTCCGTCCTCGCTTGTCGAGCGCTGTC
AGGGGCAGGGGCTCGCCGTCTATCACTGCTATGGATCGAGCGAGCATCCCACCGTGACCTCCGGCACCGTCGACGATCCG
CTCGACAAGCAGCTCCACACCGAAGGCCGGGCGATCGCGGATTGTGAAATGCGCTTCGTCGATGACGACGGCAACGATGT
GCCCGCCGGTTGCGATGGCGAAATCTGCACGCGCGGACCCGACCTCTTCCTGGGCTATCTTGACCCCGCATTGAACACGG
ACGCCTTCCTTCCGGGGGGCTGGTATCGTACCGGCGACATCGGCCGCCTCGACGACGACGGTTATCTGCTGATTACCGAT
CGCAAGAAAGACATCATCATCCGCGGTGGCGAGAATATCTCGTCAAAGGAAGTCGAAGCGCTGCTGCTCGCGCATCCGGC
CATCGTCGATGCGGCGGCGGTGGCGGCGCCGGACGACCGTATGGGCGAAGTGGTCCGCGCCTGCGTCGTTCTGGCGCCAG
AAGCGACGCTGACGATCGACGAATTGCGCGAACATTTCTTCGCGGCCGGCATTGCGAAACAAAAGACTCCCGAACGGCTG
ACCATCCTGCCCGAGCTTCCGCGCAATGCATCGGGCAAGGTGTTGAAGCATGAATTGCGCCGCTGTTCCTGA

Upstream 100 bases:

>100_bases
TCGAAGGCGCGGTGGCGCACTGGCGCCTTCACCTTCGCAACGCCAATGAAACCTGGGCGCAGCGCGGTGAGGGCGAGCGG
ATCGTCGATTCCCTGCACGC

Downstream 100 bases:

>100_bases
ACCGGCGCCATCGAGCGTCAATTCCGCATGATGAAGCCGCCATCGACGTTCAGCGCCTGCCCCGTGATCCAGTCGCCCGC
GGGCGAGCAAAGGAGCAGGA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MTASFIDAMIAAAARAPQASLIVASDMRPCDTTLGEVTAAGRRMGSRMTKAGVRPGDVVGCMLPNWREWLVVAVAAAQAG
AVMLPIVTIYGAKELAFILRQSKARWLFTPDHFRGTDYGRLVAECGELPMLERHIRAGEDFGALESDGAMADPVPSDPDE
LALLVYTSGTTADPKGVMHSARTLLAEMASMREMRREKDEDIVISPWPPGHVAGALSMYRFLCQGTPLVLMDQWDAALAA
ELIDRHKPTSSSGTPFHLSGLLAAADVHGHDLSSLRQYLVGAAPVPSSLVERCQGQGLAVYHCYGSSEHPTVTSGTVDDP
LDKQLHTEGRAIADCEMRFVDDDGNDVPAGCDGEICTRGPDLFLGYLDPALNTDAFLPGGWYRTGDIGRLDDDGYLLITD
RKKDIIIRGGENISSKEVEALLLAHPAIVDAAAVAAPDDRMGEVVRACVVLAPEATLTIDELREHFFAAGIAKQKTPERL
TILPELPRNASGKVLKHELRRCS

Sequences:

>Translated_503_residues
MTASFIDAMIAAAARAPQASLIVASDMRPCDTTLGEVTAAGRRMGSRMTKAGVRPGDVVGCMLPNWREWLVVAVAAAQAG
AVMLPIVTIYGAKELAFILRQSKARWLFTPDHFRGTDYGRLVAECGELPMLERHIRAGEDFGALESDGAMADPVPSDPDE
LALLVYTSGTTADPKGVMHSARTLLAEMASMREMRREKDEDIVISPWPPGHVAGALSMYRFLCQGTPLVLMDQWDAALAA
ELIDRHKPTSSSGTPFHLSGLLAAADVHGHDLSSLRQYLVGAAPVPSSLVERCQGQGLAVYHCYGSSEHPTVTSGTVDDP
LDKQLHTEGRAIADCEMRFVDDDGNDVPAGCDGEICTRGPDLFLGYLDPALNTDAFLPGGWYRTGDIGRLDDDGYLLITD
RKKDIIIRGGENISSKEVEALLLAHPAIVDAAAVAAPDDRMGEVVRACVVLAPEATLTIDELREHFFAAGIAKQKTPERL
TILPELPRNASGKVLKHELRRCS
>Mature_502_residues
TASFIDAMIAAAARAPQASLIVASDMRPCDTTLGEVTAAGRRMGSRMTKAGVRPGDVVGCMLPNWREWLVVAVAAAQAGA
VMLPIVTIYGAKELAFILRQSKARWLFTPDHFRGTDYGRLVAECGELPMLERHIRAGEDFGALESDGAMADPVPSDPDEL
ALLVYTSGTTADPKGVMHSARTLLAEMASMREMRREKDEDIVISPWPPGHVAGALSMYRFLCQGTPLVLMDQWDAALAAE
LIDRHKPTSSSGTPFHLSGLLAAADVHGHDLSSLRQYLVGAAPVPSSLVERCQGQGLAVYHCYGSSEHPTVTSGTVDDPL
DKQLHTEGRAIADCEMRFVDDDGNDVPAGCDGEICTRGPDLFLGYLDPALNTDAFLPGGWYRTGDIGRLDDDGYLLITDR
KKDIIIRGGENISSKEVEALLLAHPAIVDAAAVAAPDDRMGEVVRACVVLAPEATLTIDELREHFFAAGIAKQKTPERLT
ILPELPRNASGKVLKHELRRCS

Specific function: Catalyzes the esterification, concomitant with transport, of exogenous fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. It has a low activity on medium or long chain fatty acids and is ma

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Membrane; Peripheral membrane protein. Note=Partially membrane-associated [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=535, Percent_Identity=27.2897196261682, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI38505220, Length=500, Percent_Identity=29.2, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI122937307, Length=500, Percent_Identity=28.2, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI115511026, Length=501, Percent_Identity=26.9461077844311, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI42544132, Length=503, Percent_Identity=26.0437375745527, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI157311624, Length=469, Percent_Identity=26.4392324093817, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI157311622, Length=469, Percent_Identity=26.4392324093817, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI58082049, Length=482, Percent_Identity=26.7634854771784, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI187761345, Length=474, Percent_Identity=26.1603375527426, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI187761343, Length=474, Percent_Identity=26.1603375527426, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI27477105, Length=502, Percent_Identity=26.2948207171315, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI40807491, Length=456, Percent_Identity=23.6842105263158, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI28416953, Length=514, Percent_Identity=24.5136186770428, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI57165412, Length=473, Percent_Identity=25.369978858351, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI57165410, Length=473, Percent_Identity=25.369978858351, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI42794756, Length=462, Percent_Identity=25.1082251082251, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI42794760, Length=457, Percent_Identity=24.507658643326, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI42794758, Length=457, Percent_Identity=24.507658643326, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI83745141, Length=476, Percent_Identity=23.3193277310924, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI8923896, Length=125, Percent_Identity=34.4, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI253970489, Length=125, Percent_Identity=34.4, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI145693145, Length=497, Percent_Identity=35.4124748490946, Blast_Score=278, Evalue=7e-76,
Organism=Escherichia coli, GI1788107, Length=518, Percent_Identity=27.2200772200772, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI1786810, Length=493, Percent_Identity=28.6004056795132, Blast_Score=165, Evalue=8e-42,
Organism=Escherichia coli, GI221142682, Length=495, Percent_Identity=28.0808080808081, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789201, Length=470, Percent_Identity=27.0212765957447, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1790505, Length=512, Percent_Identity=24.0234375, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788595, Length=351, Percent_Identity=26.2108262108262, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786801, Length=434, Percent_Identity=24.4239631336406, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17560308, Length=538, Percent_Identity=29.7397769516729, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI71994690, Length=516, Percent_Identity=28.6821705426357, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI71994703, Length=516, Percent_Identity=28.8759689922481, Blast_Score=174, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI71994694, Length=516, Percent_Identity=28.6821705426357, Blast_Score=174, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17559526, Length=479, Percent_Identity=26.5135699373695, Blast_Score=148, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI32563687, Length=508, Percent_Identity=26.5748031496063, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17558820, Length=478, Percent_Identity=26.5690376569038, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=485, Percent_Identity=25.5670103092783, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=487, Percent_Identity=25.0513347022587, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17560140, Length=492, Percent_Identity=25.4065040650407, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71996755, Length=482, Percent_Identity=23.8589211618257, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17531443, Length=488, Percent_Identity=23.7704918032787, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71985884, Length=390, Percent_Identity=25.3846153846154, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71982997, Length=545, Percent_Identity=24.0366972477064, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71983001, Length=544, Percent_Identity=24.0808823529412, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=471, Percent_Identity=25.6900212314225, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323182, Length=529, Percent_Identity=25.1417769376182, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6319264, Length=146, Percent_Identity=32.8767123287671, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24581924, Length=489, Percent_Identity=28.6298568507157, Blast_Score=155, Evalue=6e-38,
Organism=Drosophila melanogaster, GI21355181, Length=475, Percent_Identity=27.3684210526316, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI18859661, Length=482, Percent_Identity=28.0082987551867, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24648260, Length=529, Percent_Identity=23.2514177693762, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24648253, Length=478, Percent_Identity=22.8033472803347, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356947, Length=492, Percent_Identity=25, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648255, Length=478, Percent_Identity=23.0125523012552, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI19922652, Length=521, Percent_Identity=26.1036468330134, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21356441, Length=476, Percent_Identity=21.8487394957983, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653035, Length=476, Percent_Identity=23.109243697479, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24656500, Length=510, Percent_Identity=22.7450980392157, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI20130357, Length=349, Percent_Identity=23.2091690544413, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI17933690, Length=457, Percent_Identity=25.164113785558, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648257, Length=377, Percent_Identity=23.6074270557029, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19921316, Length=474, Percent_Identity=23.6286919831224, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI281365686, Length=456, Percent_Identity=21.4912280701754, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI161076582, Length=484, Percent_Identity=21.900826446281, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21358303, Length=450, Percent_Identity=20, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 54161; Mature: 54030

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASFIDAMIAAAARAPQASLIVASDMRPCDTTLGEVTAAGRRMGSRMTKAGVRPGDVVG
CCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
CMLPNWREWLVVAVAAAQAGAVMLPIVTIYGAKELAFILRQSKARWLFTPDHFRGTDYGR
ECCCCHHHHHHHHHHHHCCCEEEEHHHHHHCHHHHHHHHHHCCCCEEECCCCCCCCCHHH
LVAECGELPMLERHIRAGEDFGALESDGAMADPVPSDPDELALLVYTSGTTADPKGVMHS
HHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHH
ARTLLAEMASMREMRREKDEDIVISPWPPGHVAGALSMYRFLCQGTPLVLMDQWDAALAA
HHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHH
ELIDRHKPTSSSGTPFHLSGLLAAADVHGHDLSSLRQYLVGAAPVPSSLVERCQGQGLAV
HHHHHCCCCCCCCCCEEHHHHHEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCEEE
YHCYGSSEHPTVTSGTVDDPLDKQLHTEGRAIADCEMRFVDDDGNDVPAGCDGEICTRGP
EEEECCCCCCEECCCCCCCCHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCC
DLFLGYLDPALNTDAFLPGGWYRTGDIGRLDDDGYLLITDRKKDIIIRGGENISSKEVEA
CEEEEECCCCCCCCCCCCCCCEECCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHH
LLLAHPAIVDAAAVAAPDDRMGEVVRACVVLAPEATLTIDELREHFFAAGIAKQKTPERL
HHHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCEE
TILPELPRNASGKVLKHELRRCS
EEECCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TASFIDAMIAAAARAPQASLIVASDMRPCDTTLGEVTAAGRRMGSRMTKAGVRPGDVVG
CHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
CMLPNWREWLVVAVAAAQAGAVMLPIVTIYGAKELAFILRQSKARWLFTPDHFRGTDYGR
ECCCCHHHHHHHHHHHHCCCEEEEHHHHHHCHHHHHHHHHHCCCCEEECCCCCCCCCHHH
LVAECGELPMLERHIRAGEDFGALESDGAMADPVPSDPDELALLVYTSGTTADPKGVMHS
HHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHH
ARTLLAEMASMREMRREKDEDIVISPWPPGHVAGALSMYRFLCQGTPLVLMDQWDAALAA
HHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHH
ELIDRHKPTSSSGTPFHLSGLLAAADVHGHDLSSLRQYLVGAAPVPSSLVERCQGQGLAV
HHHHHCCCCCCCCCCEEHHHHHEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCEEE
YHCYGSSEHPTVTSGTVDDPLDKQLHTEGRAIADCEMRFVDDDGNDVPAGCDGEICTRGP
EEEECCCCCCEECCCCCCCCHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCC
DLFLGYLDPALNTDAFLPGGWYRTGDIGRLDDDGYLLITDRKKDIIIRGGENISSKEVEA
CEEEEECCCCCCCCCCCCCCCEECCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHH
LLLAHPAIVDAAAVAAPDDRMGEVVRACVVLAPEATLTIDELREHFFAAGIAKQKTPERL
HHHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCEE
TILPELPRNASGKVLKHELRRCS
EEECCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 7984428; 12535077 [H]