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The map label for this gene is lexA

Identifier: 103486322

GI number: 103486322

Start: 838302

End: 838985

Strand: Reverse

Name: lexA

Synonym: Sala_0832

Alternate gene names: 103486322

Gene position: 838985-838302 (Counterclockwise)

Preceding gene: 103486323

Following gene: 103486320

Centisome position: 25.08

GC content: 63.3

Gene sequence:

>684_bases
ATGTTGACCGCGAAGCAGCATGAACTGCTCCACTTCATCCAGCAGCGCCTCGACGCCAGCGGCATCTCGCCGTCGTTCGA
GGAGATGAAGGAAGCGCTGGGCCTCAAGTCGAAATCGGGCATCCATCGGCTGATAAGTGCTTTGGAAGAAAGGGGTTTTC
TCCGCCGCCTGCCCAACCGCGCGCGCGCGCTGGAGGTGCTCAAGCTGCCCGATACCGCGAAGTCCGTCGTGACCAACAAT
CGCGACAATGTCGTCCAGCTGCGCAAGGCGCCGTCGGCGCTCAAGCCGATCGCCGCGAACGACATTATCGAAGTGCCGCT
GCACGGCAGGATCGCCGCGGGCGTCCCGATCGAGGCGTTCGAGGATCATGAGCAACTCGCCGTTCCCGCCGCGCTGCTCG
GTTCGGGCGAACATTATGCGCTCGAAGTGTCGGGCGACTCGATGGTCGAGGCGGGGATTTTCGACGGCGATTATGCGCTG
ATCCAGAAAGCGGACACCGCGCGCGAGGGCGACATCGTCGTCGCGCTCGTCGACGGGCAGGATGCGACGCTCAAATTCTT
TCGGCGCGAAGGCAAGATGATCCGGCTCGACCCTGCAAACAGCGCCTATGAACCGCAACGCTACCCGGCCGAGCGCGTCA
TCGTCCAGGGGCGTCTGTCGGGACTGCTTCGTCGTTACCACTGA

Upstream 100 bases:

>100_bases
CGGCGGACTATATCGCCATCGCTTGACAAGTTCCGCTTTGTTCCACTATCGTTCCCGCTATGTCCGGCCTGACGCTCCAG
GCCTTCATGGAGAACGACCG

Downstream 100 bases:

>100_bases
CCTGCTGCGGGCGACGCCACGGATGCTCGGCACCGGACTTTAGCGTGGCGACGGCTTTGGGCCGTTCGCCAAGATAGAGC
GCGAGCCCGCCGGTTTCGGC

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MLTAKQHELLHFIQQRLDASGISPSFEEMKEALGLKSKSGIHRLISALEERGFLRRLPNRARALEVLKLPDTAKSVVTNN
RDNVVQLRKAPSALKPIAANDIIEVPLHGRIAAGVPIEAFEDHEQLAVPAALLGSGEHYALEVSGDSMVEAGIFDGDYAL
IQKADTAREGDIVVALVDGQDATLKFFRREGKMIRLDPANSAYEPQRYPAERVIVQGRLSGLLRRYH

Sequences:

>Translated_227_residues
MLTAKQHELLHFIQQRLDASGISPSFEEMKEALGLKSKSGIHRLISALEERGFLRRLPNRARALEVLKLPDTAKSVVTNN
RDNVVQLRKAPSALKPIAANDIIEVPLHGRIAAGVPIEAFEDHEQLAVPAALLGSGEHYALEVSGDSMVEAGIFDGDYAL
IQKADTAREGDIVVALVDGQDATLKFFRREGKMIRLDPANSAYEPQRYPAERVIVQGRLSGLLRRYH
>Mature_227_residues
MLTAKQHELLHFIQQRLDASGISPSFEEMKEALGLKSKSGIHRLISALEERGFLRRLPNRARALEVLKLPDTAKSVVTNN
RDNVVQLRKAPSALKPIAANDIIEVPLHGRIAAGVPIEAFEDHEQLAVPAALLGSGEHYALEVSGDSMVEAGIFDGDYAL
IQKADTAREGDIVVALVDGQDATLKFFRREGKMIRLDPANSAYEPQRYPAERVIVQGRLSGLLRRYH

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=207, Percent_Identity=27.536231884058, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787431, Length=127, Percent_Identity=35.4330708661417, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_SPHAL (Q1GUX2)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_615883.1
- ProteinModelPortal:   Q1GUX2
- SMR:   Q1GUX2
- MEROPS:   S24.001
- GeneID:   4080040
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0832
- NMPDR:   fig|317655.9.peg.778
- HOGENOM:   HBG679610
- OMA:   LMFIHER
- ProtClustDB:   PRK00215
- BioCyc:   SALA317655:SALA_0832-MONOMER
- BRENDA:   3.4.21.88
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 25049; Mature: 25049

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: ACT_SITE 147-147 ACT_SITE 185-185

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTAKQHELLHFIQQRLDASGISPSFEEMKEALGLKSKSGIHRLISALEERGFLRRLPNR
CCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHCCCH
ARALEVLKLPDTAKSVVTNNRDNVVQLRKAPSALKPIAANDIIEVPLHGRIAAGVPIEAF
HHHHHEEECCCHHHHHHCCCCCCEEEEHHCCCHHCCCCCCCEEECCCCCEEECCCCHHHH
EDHEQLAVPAALLGSGEHYALEVSGDSMVEAGIFDGDYALIQKADTAREGDIVVALVDGQ
CCCHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCHHHHCCCCCCCCCCEEEEEECCC
DATLKFFRREGKMIRLDPANSAYEPQRYPAERVIVQGRLSGLLRRYH
CHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTAKQHELLHFIQQRLDASGISPSFEEMKEALGLKSKSGIHRLISALEERGFLRRLPNR
CCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHCCCH
ARALEVLKLPDTAKSVVTNNRDNVVQLRKAPSALKPIAANDIIEVPLHGRIAAGVPIEAF
HHHHHEEECCCHHHHHHCCCCCCEEEEHHCCCHHCCCCCCCEEECCCCCEEECCCCHHHH
EDHEQLAVPAALLGSGEHYALEVSGDSMVEAGIFDGDYALIQKADTAREGDIVVALVDGQ
CCCHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCHHHHCCCCCCCCCCEEEEEECCC
DATLKFFRREGKMIRLDPANSAYEPQRYPAERVIVQGRLSGLLRRYH
CHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA