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The map label for this gene is gltX1

Identifier: 103486320

GI number: 103486320

Start: 834658

End: 836130

Strand: Reverse

Name: gltX1

Synonym: Sala_0830

Alternate gene names: 103486320

Gene position: 836130-834658 (Counterclockwise)

Preceding gene: 103486322

Following gene: 103486319

Centisome position: 25.0

GC content: 68.09

Gene sequence:

>1473_bases
GTGGCAACCGAAAATCCGACCAGAGTGGCAACAGCCACGCCCGAAGCGACCGGCGCCGACGTGGTGACGCGCTTCGCCCC
CTCGCCCACCGGCTACCTCCACATCGGCGGCGCGCGCACCGCGCTGTTCAACTGGCTGTTCGCGCGCCATCATGGCGGCA
AGTTCCTGCTGCGGATCGAGGATACCGACCGTGCGCGATCGACCGACGCGGCGATCGACGCGATCCTCGACGGGATGCGC
TGGCTGGAACTCGACTGGGACGGCGAGACGGTGTTCCAGTTCGCGCGCGCGCCGCGCCATGCCGAGGTCGCGCACGACCT
GCTCGCACGCGGCGGCGCCTATCGCTGTTACCTGACGCAGGACGAGCTGGCGGCGATGCGCGCCGAAGCGCAGGAAAAGC
GTCAGCCTTTCCGCGTCCGCAGCCCGTGGCGCGACCGCGACGACGGCGACCCCGCGGCGCCGCACGTCCTTCGTCTCCGC
GCGCCGCAGGATGGCGCCGTGACGATTGCCGACAAGGTGCAGGGCGAGGTTACGGTGCAGAATGCCGAGCTGGACGATTT
CATCCTGCTGCGCAGCGACGGCACGCCGACCTATATGCTGAGCGTCGTCGTCGATGATAATGACATGGGCGTCACCCATG
TCATCCGCGGCGACGATCACCTCAACAACGCCTTTCGCCAATTGGCCCTGATCCGCGCGATGGACTGGCGCGAGCCGGTC
TATGCGCACGTCCCGCTGATCCACGGCGCCGACGGGACGAAGCTGTCGAAACGGCACGGCGCGCTCGGCGTCGATGCCTA
TCGCGACGCGATGGGATATTTGCCCGAAGCGGTGAACAATTACCTCCTCCGCCTGGGCTGGGGACATGGCGATGACGAGA
TCATCAGCCGCGCCCAGGCGATCGAATGGTTCGACCTCGATCATGTCGGCCGCTCGCCCTCGCGCTTCGATTTCAAGAAG
CTGGAGAATCTGAACGGTCATTATCTTCGCGAAGCCGACGATGCGCGGCTCGCCGGCCTCGTCGCGCCGCGCATCGAGGC
GCTCGTCGGACGCGCGCTGGACAATGCCGACCGCGACCTGCTGACGCAGGCGATGGCGGCGCTCAAGCCGCGGGCCAAGA
CGCTGGGCGAGATCGCCGAGGGCGCGACCTTCCTCTTCGCCCCCGATCCCATGCCAGTGGACGAAAAAGCCGCCGAGGTG
CTAGCGTCCGCTCCCGAAGGCCTGCTGGCCGCGATGACGCAGCGGCTCCGCGGGCTGGACGACGACCACTGGACGAGCGA
AGATCTGGAAGCCGCGGTGCGCGCCGAGGCCGAAGCCGCCGAACTGGGGCTGGGCAAGCTGGCGCAGCCGCTACGCGCGG
CGCTGACCGGCCGCACGGTTTCCCCCGGCATTTTCGACGTGCTGCTGCTGCTGGGCCGCAAAGTCAGCCTGGCGCGACTT
GACGCAGCGCAACATTATCCGGCAGGGGCGTAG

Upstream 100 bases:

>100_bases
GCGATCAGGTGCAATGGGCGTTTGAAGCGGACTTGTCGCCATGCTAGGGGCACCCCCGATCCCGCCCGACCGGGCGGCAA
AGTGAGTGAAAGAGGCCGCG

Downstream 100 bases:

>100_bases
CCGCAACATCTCCCCGCCGTCAGAACAGGGAACCTAATATGACCGACACCGCGAAAATCACGCTGGGCGACAAGACCGTC
GACAGCCCTGTCCTGTCGGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MATENPTRVATATPEATGADVVTRFAPSPTGYLHIGGARTALFNWLFARHHGGKFLLRIEDTDRARSTDAAIDAILDGMR
WLELDWDGETVFQFARAPRHAEVAHDLLARGGAYRCYLTQDELAAMRAEAQEKRQPFRVRSPWRDRDDGDPAAPHVLRLR
APQDGAVTIADKVQGEVTVQNAELDDFILLRSDGTPTYMLSVVVDDNDMGVTHVIRGDDHLNNAFRQLALIRAMDWREPV
YAHVPLIHGADGTKLSKRHGALGVDAYRDAMGYLPEAVNNYLLRLGWGHGDDEIISRAQAIEWFDLDHVGRSPSRFDFKK
LENLNGHYLREADDARLAGLVAPRIEALVGRALDNADRDLLTQAMAALKPRAKTLGEIAEGATFLFAPDPMPVDEKAAEV
LASAPEGLLAAMTQRLRGLDDDHWTSEDLEAAVRAEAEAAELGLGKLAQPLRAALTGRTVSPGIFDVLLLLGRKVSLARL
DAAQHYPAGA

Sequences:

>Translated_490_residues
MATENPTRVATATPEATGADVVTRFAPSPTGYLHIGGARTALFNWLFARHHGGKFLLRIEDTDRARSTDAAIDAILDGMR
WLELDWDGETVFQFARAPRHAEVAHDLLARGGAYRCYLTQDELAAMRAEAQEKRQPFRVRSPWRDRDDGDPAAPHVLRLR
APQDGAVTIADKVQGEVTVQNAELDDFILLRSDGTPTYMLSVVVDDNDMGVTHVIRGDDHLNNAFRQLALIRAMDWREPV
YAHVPLIHGADGTKLSKRHGALGVDAYRDAMGYLPEAVNNYLLRLGWGHGDDEIISRAQAIEWFDLDHVGRSPSRFDFKK
LENLNGHYLREADDARLAGLVAPRIEALVGRALDNADRDLLTQAMAALKPRAKTLGEIAEGATFLFAPDPMPVDEKAAEV
LASAPEGLLAAMTQRLRGLDDDHWTSEDLEAAVRAEAEAAELGLGKLAQPLRAALTGRTVSPGIFDVLLLLGRKVSLARL
DAAQHYPAGA
>Mature_489_residues
ATENPTRVATATPEATGADVVTRFAPSPTGYLHIGGARTALFNWLFARHHGGKFLLRIEDTDRARSTDAAIDAILDGMRW
LELDWDGETVFQFARAPRHAEVAHDLLARGGAYRCYLTQDELAAMRAEAQEKRQPFRVRSPWRDRDDGDPAAPHVLRLRA
PQDGAVTIADKVQGEVTVQNAELDDFILLRSDGTPTYMLSVVVDDNDMGVTHVIRGDDHLNNAFRQLALIRAMDWREPVY
AHVPLIHGADGTKLSKRHGALGVDAYRDAMGYLPEAVNNYLLRLGWGHGDDEIISRAQAIEWFDLDHVGRSPSRFDFKKL
ENLNGHYLREADDARLAGLVAPRIEALVGRALDNADRDLLTQAMAALKPRAKTLGEIAEGATFLFAPDPMPVDEKAAEVL
ASAPEGLLAAMTQRLRGLDDDHWTSEDLEAAVRAEAEAAELGLGKLAQPLRAALTGRTVSPGIFDVLLLLGRKVSLARLD
AAQHYPAGA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=365, Percent_Identity=32.6027397260274, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI4826960, Length=307, Percent_Identity=25.7328990228013, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788743, Length=468, Percent_Identity=44.2307692307692, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI226510925, Length=254, Percent_Identity=32.2834645669291, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786895, Length=126, Percent_Identity=31.7460317460317, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=479, Percent_Identity=31.3152400835073, Blast_Score=193, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6324540, Length=498, Percent_Identity=30.1204819277108, Blast_Score=203, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6321192, Length=215, Percent_Identity=28.3720930232558, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324742, Length=116, Percent_Identity=34.4827586206897, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24665321, Length=516, Percent_Identity=31.9767441860465, Blast_Score=231, Evalue=1e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_SPHAL (Q1GUX4)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_615881.1
- ProteinModelPortal:   Q1GUX4
- SMR:   Q1GUX4
- GeneID:   4080476
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0830
- NMPDR:   fig|317655.9.peg.776
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   SALA317655:SALA_0830-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 53751; Mature: 53620

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 257-257

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATENPTRVATATPEATGADVVTRFAPSPTGYLHIGGARTALFNWLFARHHGGKFLLRIE
CCCCCCCEEEECCCCCCCCHHHHHCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEE
DTDRARSTDAAIDAILDGMRWLELDWDGETVFQFARAPRHAEVAHDLLARGGAYRCYLTQ
CCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEH
DELAAMRAEAQEKRQPFRVRSPWRDRDDGDPAAPHVLRLRAPQDGAVTIADKVQGEVTVQ
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCEEEEE
NAELDDFILLRSDGTPTYMLSVVVDDNDMGVTHVIRGDDHLNNAFRQLALIRAMDWREPV
CCCCCCEEEEECCCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCC
YAHVPLIHGADGTKLSKRHGALGVDAYRDAMGYLPEAVNNYLLRLGWGHGDDEIISRAQA
EEEECEEECCCCCEEHHHCCCCCHHHHHHHHHHCHHHHHHHEEEECCCCCHHHHHHHHHH
IEWFDLDHVGRSPSRFDFKKLENLNGHYLREADDARLAGLVAPRIEALVGRALDNADRDL
HHEECHHHCCCCCCCCCHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
LTQAMAALKPRAKTLGEIAEGATFLFAPDPMPVDEKAAEVLASAPEGLLAAMTQRLRGLD
HHHHHHHHCHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCC
DDHWTSEDLEAAVRAEAEAAELGLGKLAQPLRAALTGRTVSPGIFDVLLLLGRKVSLARL
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHH
DAAQHYPAGA
HHHCCCCCCC
>Mature Secondary Structure 
ATENPTRVATATPEATGADVVTRFAPSPTGYLHIGGARTALFNWLFARHHGGKFLLRIE
CCCCCCEEEECCCCCCCCHHHHHCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEE
DTDRARSTDAAIDAILDGMRWLELDWDGETVFQFARAPRHAEVAHDLLARGGAYRCYLTQ
CCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEH
DELAAMRAEAQEKRQPFRVRSPWRDRDDGDPAAPHVLRLRAPQDGAVTIADKVQGEVTVQ
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCEEEEE
NAELDDFILLRSDGTPTYMLSVVVDDNDMGVTHVIRGDDHLNNAFRQLALIRAMDWREPV
CCCCCCEEEEECCCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCC
YAHVPLIHGADGTKLSKRHGALGVDAYRDAMGYLPEAVNNYLLRLGWGHGDDEIISRAQA
EEEECEEECCCCCEEHHHCCCCCHHHHHHHHHHCHHHHHHHEEEECCCCCHHHHHHHHHH
IEWFDLDHVGRSPSRFDFKKLENLNGHYLREADDARLAGLVAPRIEALVGRALDNADRDL
HHEECHHHCCCCCCCCCHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
LTQAMAALKPRAKTLGEIAEGATFLFAPDPMPVDEKAAEVLASAPEGLLAAMTQRLRGLD
HHHHHHHHCHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCC
DDHWTSEDLEAAVRAEAEAAELGLGKLAQPLRAALTGRTVSPGIFDVLLLLGRKVSLARL
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHH
DAAQHYPAGA
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA