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The map label for this gene is hupB [H]

Identifier: 103486291

GI number: 103486291

Start: 806471

End: 806743

Strand: Direct

Name: hupB [H]

Synonym: Sala_0799

Alternate gene names: 103486291

Gene position: 806471-806743 (Clockwise)

Preceding gene: 103486290

Following gene: 103486292

Centisome position: 24.11

GC content: 64.84

Gene sequence:

>273_bases
ATGAACAAACAAGACCTGATCGCCGCCGTCGCCGATTCGAGCGGCCTGACCAAGGGTGATGCCAGCAAGGCCGTCGAGGC
CGTCTTCGACGCGATCACGGGCGCGCTGAAGAAGGGCGGCGAAGTCCGTCTCGTCGGCTTCGGCACCTTCGCGGTCAGCA
AGCGCAAGGCATCGACCGGTCGCAACCCGCGCACCGGCGAAACGATGACCATCGCGGCGTCGAACCAGCCGAAGTTCAAG
GCCGGCAAGGCCCTCAAGGACGCCGTCAACTAA

Upstream 100 bases:

>100_bases
CCCTTTTTTGCTTTGACAAGCCGGGGGCAAACATCGTTAGTGACGCGCCATGGCTTCGGGCCATGAGAATCATCCAACCA
AATGCAGGGGTTCCTTAGGC

Downstream 100 bases:

>100_bases
GGTCGGCGGCCCTCTTGCTGAAGAACAACTCGGGCCGTGGCGCAAGCTGCGGCCCGTTTTGCATTGGAGTCCACCCGACT
CTCCCGACCGGATTTTCGGG

Product: histone-like DNA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MNKQDLIAAVADSSGLTKGDASKAVEAVFDAITGALKKGGEVRLVGFGTFAVSKRKASTGRNPRTGETMTIAASNQPKFK
AGKALKDAVN

Sequences:

>Translated_90_residues
MNKQDLIAAVADSSGLTKGDASKAVEAVFDAITGALKKGGEVRLVGFGTFAVSKRKASTGRNPRTGETMTIAASNQPKFK
AGKALKDAVN
>Mature_90_residues
MNKQDLIAAVADSSGLTKGDASKAVEAVFDAITGALKKGGEVRLVGFGTFAVSKRKASTGRNPRTGETMTIAASNQPKFK
AGKALKDAVN

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=54.4444444444444, Blast_Score=98, Evalue=9e-23,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=53.9325842696629, Blast_Score=94, Evalue=2e-21,
Organism=Escherichia coli, GI1788005, Length=89, Percent_Identity=38.2022471910112, Blast_Score=67, Evalue=2e-13,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9240; Mature: 9240

Theoretical pI: Translated: 10.87; Mature: 10.87

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQDLIAAVADSSGLTKGDASKAVEAVFDAITGALKKGGEVRLVGFGTFAVSKRKASTG
CCCHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCC
RNPRTGETMTIAASNQPKFKAGKALKDAVN
CCCCCCCEEEEEECCCCCCCCCCHHHHHCC
>Mature Secondary Structure
MNKQDLIAAVADSSGLTKGDASKAVEAVFDAITGALKKGGEVRLVGFGTFAVSKRKASTG
CCCHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCC
RNPRTGETMTIAASNQPKFKAGKALKDAVN
CCCCCCCEEEEEECCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6299736; 10762258; 11481430; 7049166 [H]