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The map label for this gene is prfC [H]

Identifier: 103486246

GI number: 103486246

Start: 761621

End: 763270

Strand: Direct

Name: prfC [H]

Synonym: Sala_0754

Alternate gene names: 103486246

Gene position: 761621-763270 (Clockwise)

Preceding gene: 103486245

Following gene: 103486247

Centisome position: 22.77

GC content: 66.97

Gene sequence:

>1650_bases
GTGCGCCCCGTGCCAGCCCCTTTCCACTTGCCCTGTCACCTGCTAGGCGCCCGCGCCATGTCCAAGCACCCCGAACGCCG
CACCTTCGCCATCATCTCGCACCCCGACGCGGGCAAGACGACGCTGACCGAGAAACTGCTCGTCGCGGGCGGCGCGATCC
ACATCGCGGGCGAGGTGAAGGCGCGCGGCGCGGCACGGCGCGCGCGGTCGGACTGGATGAAAATCGAGCAGCAGCGCGGC
ATTTCGGTGACGTCTTCGGTGATGACCTTCGAACATGCCGGGCTGATCTTCAACCTGCTCGACACGCCGGGGCACGAGGA
TTTCAGCGAGGATACCTATCGCACGCTGACCGCGGTCGACAGCGCGGTGATGGTGATCGACGCCGCCAAGGGCATCGAGC
CGCAGACGCTGAAGCTGTTCGAGGTGTGCCGCCTGCGCTCGGTGCCGATCATCACCTTCATCAACAAGGTCGATCGCGAG
GGGCAGACGCCGTTCGAGCTGCTCGACGAGATCGCCGACCGGCTCGCGCTCGACGTCTGCCCGATGAACTGGCCGGTCGG
CATGGGCGGGCAGTTCGAGGGGATTTACGACCTGGCCGATCCGGCGCTGATGGTGCCCGGCGGCGATGCGTCGCGCATGT
ATGAGGGCGAGCGGATCGCGGTTGCGGGACTGGACGATCCCCGGCTCGCCCAACGCTTGAGCCCCGACGGGCTGGCGCTT
TTGAAAGAGGAGACCGAACTCGCGTCGGCCTGTTATGCGCCGTTCGACGTCGCGGCCTATCGCGGCGGCGACCTGACGCC
GGTGTTTTTCGGGTCGGCGCTCAAGGAGTTCGGCGTCACCGACCTGCTCGCGGGGCTCGCCAGCCACGCGCCGGGACCGC
AGCCGCAACCCGCCGAACCCGCGCCGGTGCGCCCCGACGACGATGCCGTCACCGGCTTCGTGTTCAAGGTACAGGCGAAC
ATGAACCCCGCGCACCGCGACCGCATCGCTTTCATGCGGCTCTGTTCGGGCAAGTTCGAGCGCGGGATGCGGTTGATGCA
GGGCGGGACCGGCAAGGCGATCGCGATCAGCCGCCCGATGCTGTTCCTCGCGCAGGACCGCGAAATGGCCGAGGAGGCTT
ATCCCGGCGACATCATCGGCATTCCGAACCATGGCACGCTGCGCGTCGGCGATACGCTCTCCGAACGCAGCGACATCATG
ATCACTGGCCTGCCCAATTTCGCGCCCGAGATCCTGCGCCGCGTCGCGCTGGTCGATCCGACCAAGACCAAGCAGCTGAG
GAAAGCGCTCGACGACATGGCCGAGGAGGGGATCATCCAGGTCTTCTATCCCCAGATCGGCGCCAACATGATCGTCGGCG
TCGTTGGCCAGCTGCAACTCGACGTGCTGATCAGCCGGCTGGAGGCCGAATATAAGGTCGAGGCGAAGCTCGAGGCGTCG
CCGTGGGAGACCGCGCGCTGGATCGCGAGCGACGATCCGGCGGCATTGAAGGCGTTCCAGGCTGACAATCGCGGCGCCGG
GGCGATCGACCGCGACGGCGCGCCGGTGTTCATGGCAAAGGATGCGTGGGAGGTCGGCTATGTCACCCAGCGCAATCCCG
CGATCCGCTTCACCGCGACGAAGGAACGGGTTTTCGCTCCCGCGGGTTGA

Upstream 100 bases:

>100_bases
GGGACTGAACATTTAGTGGATGAAACATCCGCTCCGCTCCAGGTTCGTATCTTTCCGCATCGAGGACCAGCCTTTGCAAC
GGTCGACAGGGGTGGCCCCC

Downstream 100 bases:

>100_bases
TTACGCGCGCCACTTTCGCCACCATGACGTCACCTTTGTTGGGCGGTGGGGGTGACGATGCGCGGATGGATAGTGGTGCT
GGCGGCGGCCGCGGGCAGTG

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MRPVPAPFHLPCHLLGARAMSKHPERRTFAIISHPDAGKTTLTEKLLVAGGAIHIAGEVKARGAARRARSDWMKIEQQRG
ISVTSSVMTFEHAGLIFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEPQTLKLFEVCRLRSVPIITFINKVDRE
GQTPFELLDEIADRLALDVCPMNWPVGMGGQFEGIYDLADPALMVPGGDASRMYEGERIAVAGLDDPRLAQRLSPDGLAL
LKEETELASACYAPFDVAAYRGGDLTPVFFGSALKEFGVTDLLAGLASHAPGPQPQPAEPAPVRPDDDAVTGFVFKVQAN
MNPAHRDRIAFMRLCSGKFERGMRLMQGGTGKAIAISRPMLFLAQDREMAEEAYPGDIIGIPNHGTLRVGDTLSERSDIM
ITGLPNFAPEILRRVALVDPTKTKQLRKALDDMAEEGIIQVFYPQIGANMIVGVVGQLQLDVLISRLEAEYKVEAKLEAS
PWETARWIASDDPAALKAFQADNRGAGAIDRDGAPVFMAKDAWEVGYVTQRNPAIRFTATKERVFAPAG

Sequences:

>Translated_549_residues
MRPVPAPFHLPCHLLGARAMSKHPERRTFAIISHPDAGKTTLTEKLLVAGGAIHIAGEVKARGAARRARSDWMKIEQQRG
ISVTSSVMTFEHAGLIFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEPQTLKLFEVCRLRSVPIITFINKVDRE
GQTPFELLDEIADRLALDVCPMNWPVGMGGQFEGIYDLADPALMVPGGDASRMYEGERIAVAGLDDPRLAQRLSPDGLAL
LKEETELASACYAPFDVAAYRGGDLTPVFFGSALKEFGVTDLLAGLASHAPGPQPQPAEPAPVRPDDDAVTGFVFKVQAN
MNPAHRDRIAFMRLCSGKFERGMRLMQGGTGKAIAISRPMLFLAQDREMAEEAYPGDIIGIPNHGTLRVGDTLSERSDIM
ITGLPNFAPEILRRVALVDPTKTKQLRKALDDMAEEGIIQVFYPQIGANMIVGVVGQLQLDVLISRLEAEYKVEAKLEAS
PWETARWIASDDPAALKAFQADNRGAGAIDRDGAPVFMAKDAWEVGYVTQRNPAIRFTATKERVFAPAG
>Mature_549_residues
MRPVPAPFHLPCHLLGARAMSKHPERRTFAIISHPDAGKTTLTEKLLVAGGAIHIAGEVKARGAARRARSDWMKIEQQRG
ISVTSSVMTFEHAGLIFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEPQTLKLFEVCRLRSVPIITFINKVDRE
GQTPFELLDEIADRLALDVCPMNWPVGMGGQFEGIYDLADPALMVPGGDASRMYEGERIAVAGLDDPRLAQRLSPDGLAL
LKEETELASACYAPFDVAAYRGGDLTPVFFGSALKEFGVTDLLAGLASHAPGPQPQPAEPAPVRPDDDAVTGFVFKVQAN
MNPAHRDRIAFMRLCSGKFERGMRLMQGGTGKAIAISRPMLFLAQDREMAEEAYPGDIIGIPNHGTLRVGDTLSERSDIM
ITGLPNFAPEILRRVALVDPTKTKQLRKALDDMAEEGIIQVFYPQIGANMIVGVVGQLQLDVLISRLEAEYKVEAKLEAS
PWETARWIASDDPAALKAFQADNRGAGAIDRDGAPVFMAKDAWEVGYVTQRNPAIRFTATKERVFAPAG

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=475, Percent_Identity=27.5789473684211, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI19923640, Length=502, Percent_Identity=25.4980079681275, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI25306283, Length=389, Percent_Identity=27.5064267352185, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI25306287, Length=292, Percent_Identity=28.7671232876712, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI157426893, Length=158, Percent_Identity=32.2784810126582, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=32.8467153284672, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI4503483, Length=156, Percent_Identity=30.1282051282051, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI310132016, Length=115, Percent_Identity=34.7826086956522, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI310110807, Length=115, Percent_Identity=34.7826086956522, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI310123363, Length=115, Percent_Identity=34.7826086956522, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1790835, Length=520, Percent_Identity=49.2307692307692, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1789738, Length=501, Percent_Identity=28.5429141716567, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1788922, Length=177, Percent_Identity=30.5084745762712, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI48994988, Length=175, Percent_Identity=33.1428571428571, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=481, Percent_Identity=26.1954261954262, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17556745, Length=478, Percent_Identity=23.8493723849372, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=34.0740740740741, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=33.8345864661654, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=33.8345864661654, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17506493, Length=170, Percent_Identity=27.0588235294118, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=482, Percent_Identity=24.6887966804979, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6322359, Length=436, Percent_Identity=25, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323320, Length=172, Percent_Identity=26.1627906976744, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6324707, Length=156, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=156, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319282, Length=139, Percent_Identity=32.3741007194245, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24582462, Length=481, Percent_Identity=27.6507276507277, Blast_Score=159, Evalue=7e-39,
Organism=Drosophila melanogaster, GI221458488, Length=498, Percent_Identity=25.5020080321285, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI78706572, Length=156, Percent_Identity=32.6923076923077, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24585711, Length=183, Percent_Identity=30.6010928961749, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585713, Length=183, Percent_Identity=30.6010928961749, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585709, Length=160, Percent_Identity=31.25, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24656849, Length=132, Percent_Identity=31.8181818181818, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59753; Mature: 59753

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPVPAPFHLPCHLLGARAMSKHPERRTFAIISHPDAGKTTLTEKLLVAGGAIHIAGEVK
CCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEECCHH
ARGAARRARSDWMKIEQQRGISVTSSVMTFEHAGLIFNLLDTPGHEDFSEDTYRTLTAVD
HHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHC
SAVMVIDAAKGIEPQTLKLFEVCRLRSVPIITFINKVDREGQTPFELLDEIADRLALDVC
CEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEC
PMNWPVGMGGQFEGIYDLADPALMVPGGDASRMYEGERIAVAGLDDPRLAQRLSPDGLAL
CCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHCCCCCEEEEECCCCHHHHHHCCCCCCEE
LKEETELASACYAPFDVAAYRGGDLTPVFFGSALKEFGVTDLLAGLASHAPGPQPQPAEP
HHHHHHHHHHHCCCCHHHEECCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC
APVRPDDDAVTGFVFKVQANMNPAHRDRIAFMRLCSGKFERGMRLMQGGTGKAIAISRPM
CCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCHHHHCHHHHCCCCCCEEEEECCE
LFLAQDREMAEEAYPGDIIGIPNHGTLRVGDTLSERSDIMITGLPNFAPEILRRVALVDP
EEEECCHHHHHHCCCCCEEECCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
TKTKQLRKALDDMAEEGIIQVFYPQIGANMIVGVVGQLQLDVLISRLEAEYKVEAKLEAS
HHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECC
PWETARWIASDDPAALKAFQADNRGAGAIDRDGAPVFMAKDAWEVGYVTQRNPAIRFTAT
CCCCEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCEECEEECCCCEEEEEEC
KERVFAPAG
CCEEECCCC
>Mature Secondary Structure
MRPVPAPFHLPCHLLGARAMSKHPERRTFAIISHPDAGKTTLTEKLLVAGGAIHIAGEVK
CCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEECCHH
ARGAARRARSDWMKIEQQRGISVTSSVMTFEHAGLIFNLLDTPGHEDFSEDTYRTLTAVD
HHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHC
SAVMVIDAAKGIEPQTLKLFEVCRLRSVPIITFINKVDREGQTPFELLDEIADRLALDVC
CEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEC
PMNWPVGMGGQFEGIYDLADPALMVPGGDASRMYEGERIAVAGLDDPRLAQRLSPDGLAL
CCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHCCCCCEEEEECCCCHHHHHHCCCCCCEE
LKEETELASACYAPFDVAAYRGGDLTPVFFGSALKEFGVTDLLAGLASHAPGPQPQPAEP
HHHHHHHHHHHCCCCHHHEECCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC
APVRPDDDAVTGFVFKVQANMNPAHRDRIAFMRLCSGKFERGMRLMQGGTGKAIAISRPM
CCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCHHHHCHHHHCCCCCCEEEEECCE
LFLAQDREMAEEAYPGDIIGIPNHGTLRVGDTLSERSDIMITGLPNFAPEILRRVALVDP
EEEECCHHHHHHCCCCCEEECCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
TKTKQLRKALDDMAEEGIIQVFYPQIGANMIVGVVGQLQLDVLISRLEAEYKVEAKLEAS
HHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECC
PWETARWIASDDPAALKAFQADNRGAGAIDRDGAPVFMAKDAWEVGYVTQRNPAIRFTAT
CCCCEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCEECEEECCCCEEEEEEC
KERVFAPAG
CCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA