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The map label for this gene is gno [H]

Identifier: 103485994

GI number: 103485994

Start: 519303

End: 520034

Strand: Reverse

Name: gno [H]

Synonym: Sala_0501

Alternate gene names: 103485994

Gene position: 520034-519303 (Counterclockwise)

Preceding gene: 103485996

Following gene: 103485993

Centisome position: 15.55

GC content: 67.08

Gene sequence:

>732_bases
ATGTCCCATCCCGCAGCTGTCATCATCGGAGCCGGAGACGCCACCGGCGGCGCGATCGCGCGCGCATTTGCCGCCGCAGG
CCTTACCGCCTGCGTCAACCGGCGGGCCCGCAACGCCGACCAGCTCGAAGCCCTCGCCCAGTCGATCCGCGACGCGGGAC
ACAAGGCGCGCGCTTTCCCCGCCGACGCCCGCGACGAGAACGCAATGATCGAGCTGTTCGACACGGTCGAGGCCGAGGTC
GGGCCGGTCGAGGTGGCGGTGTTCAACATCGGCGCCAACGTCAATTTCCCGATCGCCGAAACGACGCTGCGCGTTTACAC
AAAGGTGTGGGAAATGGCGTGTCTCGGCGGCTTCCTGATGGGGCGCGAGGCGGCAAAGCGCATGGCGCCGCGCGGCCGCG
GCACGATCATCTTCACCGGCGCAACCGCCAGCTTGCGCGGCGGATCGGGTTACGCCGCCTTTTCGGGTGCGAAGGGGGCG
CTCAGGATGCTCGCCCAGTCGATGGCGCGCGAACTGGGGCCGCGGGGCATCCATGTCGCGCACACGGTGATCGACGGCGC
GATCGACACCGATTTCATCAGGGGCCGCCACCCCGATTTCGACAATGCAAGGGCGCAGGACCTGATCCTGAACCCAGAGG
CCATCGCCGCCAATTATGTGACGCTCCACAAACAGCCGAAAAGCGCGTGGACGCACGAACTCGACCTTCGCCCGTGGGGA
GAAACATGGTGA

Upstream 100 bases:

>100_bases
ACAGGTCTGCGCGTTTGGGCATCGTTCCAATCAACCGGAAAATTATCACTTGTCACTATGATTATCATAGTTACTATCGG
GTGCAAGAGGAGAGTCGCGC

Downstream 100 bases:

>100_bases
CCAAAAGCCTCGAACTCATCTTTGATTTCGGCAGCCCCAACGCCTATCTGGCGATGAAGGCGCTCCCCGACCTGCTCGAC
CGCACCGGCGCCGATCTTGT

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: 5-keto-D-gluconate 5-reductase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSHPAAVIIGAGDATGGAIARAFAAAGLTACVNRRARNADQLEALAQSIRDAGHKARAFPADARDENAMIELFDTVEAEV
GPVEVAVFNIGANVNFPIAETTLRVYTKVWEMACLGGFLMGREAAKRMAPRGRGTIIFTGATASLRGGSGYAAFSGAKGA
LRMLAQSMARELGPRGIHVAHTVIDGAIDTDFIRGRHPDFDNARAQDLILNPEAIAANYVTLHKQPKSAWTHELDLRPWG
ETW

Sequences:

>Translated_243_residues
MSHPAAVIIGAGDATGGAIARAFAAAGLTACVNRRARNADQLEALAQSIRDAGHKARAFPADARDENAMIELFDTVEAEV
GPVEVAVFNIGANVNFPIAETTLRVYTKVWEMACLGGFLMGREAAKRMAPRGRGTIIFTGATASLRGGSGYAAFSGAKGA
LRMLAQSMARELGPRGIHVAHTVIDGAIDTDFIRGRHPDFDNARAQDLILNPEAIAANYVTLHKQPKSAWTHELDLRPWG
ETW
>Mature_242_residues
SHPAAVIIGAGDATGGAIARAFAAAGLTACVNRRARNADQLEALAQSIRDAGHKARAFPADARDENAMIELFDTVEAEVG
PVEVAVFNIGANVNFPIAETTLRVYTKVWEMACLGGFLMGREAAKRMAPRGRGTIIFTGATASLRGGSGYAAFSGAKGAL
RMLAQSMARELGPRGIHVAHTVIDGAIDTDFIRGRHPDFDNARAQDLILNPEAIAANYVTLHKQPKSAWTHELDLRPWGE
TW

Specific function: Involved in the non-phosphorylative, ketogenic oxidation of glucose and oxidizes gluconate to 5-ketogluconate. Dependent on NADP, almost inactive with NAD [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Escherichia coli, GI2367175, Length=179, Percent_Identity=29.608938547486, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=180, Percent_Identity=31.1111111111111, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.69 [H]

Molecular weight: Translated: 25905; Mature: 25774

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHPAAVIIGAGDATGGAIARAFAAAGLTACVNRRARNADQLEALAQSIRDAGHKARAFP
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCC
ADARDENAMIELFDTVEAEVGPVEVAVFNIGANVNFPIAETTLRVYTKVWEMACLGGFLM
CCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GREAAKRMAPRGRGTIIFTGATASLRGGSGYAAFSGAKGALRMLAQSMARELGPRGIHVA
HHHHHHHCCCCCCCEEEEECCCEEECCCCCCEECCCCHHHHHHHHHHHHHHCCCCCEEEH
HTVIDGAIDTDFIRGRHPDFDNARAQDLILNPEAIAANYVTLHKQPKSAWTHELDLRPWG
HHHHHCCCCHHHHCCCCCCCCCCCCCCEEECCHHHHEEEEEEECCCCCCCCEECCCCCCC
ETW
CCC
>Mature Secondary Structure 
SHPAAVIIGAGDATGGAIARAFAAAGLTACVNRRARNADQLEALAQSIRDAGHKARAFP
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCC
ADARDENAMIELFDTVEAEVGPVEVAVFNIGANVNFPIAETTLRVYTKVWEMACLGGFLM
CCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GREAAKRMAPRGRGTIIFTGATASLRGGSGYAAFSGAKGALRMLAQSMARELGPRGIHVA
HHHHHHHCCCCCCCEEEEECCCEEECCCCCCEECCCCHHHHHHHHHHHHHHCCCCCEEEH
HTVIDGAIDTDFIRGRHPDFDNARAQDLILNPEAIAANYVTLHKQPKSAWTHELDLRPWG
HHHHHCCCCHHHHCCCCCCCCCCCCCCEEECCHHHHEEEEEEECCCCCCCCEECCCCCCC
ETW
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751271 [H]