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The map label for this gene is ruvC

Identifier: 103485831

GI number: 103485831

Start: 340421

End: 340891

Strand: Reverse

Name: ruvC

Synonym: Sala_0336

Alternate gene names: 103485831

Gene position: 340891-340421 (Counterclockwise)

Preceding gene: 103485832

Following gene: 103485830

Centisome position: 10.19

GC content: 69.64

Gene sequence:

>471_bases
ATGATCATCCTCGGCCTCGACCCCTCGCTCAGTTGCACCGGCTGGGGCCTCATCCGCGTCGAGGGCAGCCGGATCAGCCA
TATCGCCAACGGCCAGATCAAGACCGACGCCAAGGCCGCCCTCCCCGACCGCCTCGTGCATCTCGACACCGCGCTCGCTG
CGGTGATCGCCGATCATGCGCCAAAATGCGCCGCAGTCGAGGAGGTGTTCGTCAACGACAATCCGCAATCGACGCTCAAG
CTCGCGCACGCGCGCGGCGTGGTCCTGCTCGGCTGTGCGCGCGGCGGGCTGACGGTCACGCAATATGCCCCGCGCCTCGT
CAAGAAAGCGATCGTCGGCACCGGCGGCGCAGCGAAGGGGCAGGTGCAGGCGATGCTGGGCGTGCTGCTGCCGGGCACCA
AGGTCGCGGGGCCGGATGCGGCGGACGCGCTGGCGGTCGCCATCTGCCACGCGAACCACCGGCGGCGTTGA

Upstream 100 bases:

>100_bases
GCGCTGACGGAGGGGGGGTGCGGCCTGCGACCTCGCTTGAGGCCGCGAGCCCCCTCCACCACCCTGCGGGTGGTCCCCCT
CCCCGTTCCGGGGAGGAATG

Downstream 100 bases:

>100_bases
GGAACGGCGGTTTTGGGGTGGGGAGCGGACGTAGCCCTCTCCCCGTCAGGGGAGAGGATAGCGCAGCTTGCTCCGCCAGG
AGCTAGCGAAGCTTGGAGAG

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MIILGLDPSLSCTGWGLIRVEGSRISHIANGQIKTDAKAALPDRLVHLDTALAAVIADHAPKCAAVEEVFVNDNPQSTLK
LAHARGVVLLGCARGGLTVTQYAPRLVKKAIVGTGGAAKGQVQAMLGVLLPGTKVAGPDAADALAVAICHANHRRR

Sequences:

>Translated_156_residues
MIILGLDPSLSCTGWGLIRVEGSRISHIANGQIKTDAKAALPDRLVHLDTALAAVIADHAPKCAAVEEVFVNDNPQSTLK
LAHARGVVLLGCARGGLTVTQYAPRLVKKAIVGTGGAAKGQVQAMLGVLLPGTKVAGPDAADALAVAICHANHRRR
>Mature_156_residues
MIILGLDPSLSCTGWGLIRVEGSRISHIANGQIKTDAKAALPDRLVHLDTALAAVIADHAPKCAAVEEVFVNDNPQSTLK
LAHARGVVLLGCARGGLTVTQYAPRLVKKAIVGTGGAAKGQVQAMLGVLLPGTKVAGPDAADALAVAICHANHRRR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=149, Percent_Identity=42.2818791946309, Blast_Score=91, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_SPHAL (Q1GWB3)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_615392.1
- ProteinModelPortal:   Q1GWB3
- SMR:   Q1GWB3
- GeneID:   4081034
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0336
- NMPDR:   fig|317655.9.peg.311
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- ProtClustDB:   PRK00039
- BioCyc:   SALA317655:SALA_0336-MONOMER
- BRENDA:   3.1.22.4
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 16076; Mature: 16076

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILGLDPSLSCTGWGLIRVEGSRISHIANGQIKTDAKAALPDRLVHLDTALAAVIADHA
CEEEECCCCCCCCCEEEEEECCCEEEEEECCEECCCCHHHCCHHHHHHHHHHHHHHHHCC
PKCAAVEEVFVNDNPQSTLKLAHARGVVLLGCARGGLTVTQYAPRLVKKAIVGTGGAAKG
CCCHHHHHEEECCCCHHHHHHHCCCCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCCCCH
QVQAMLGVLLPGTKVAGPDAADALAVAICHANHRRR
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIILGLDPSLSCTGWGLIRVEGSRISHIANGQIKTDAKAALPDRLVHLDTALAAVIADHA
CEEEECCCCCCCCCEEEEEECCCEEEEEECCEECCCCHHHCCHHHHHHHHHHHHHHHHCC
PKCAAVEEVFVNDNPQSTLKLAHARGVVLLGCARGGLTVTQYAPRLVKKAIVGTGGAAKG
CCCHHHHHEEECCCCHHHHHHHCCCCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCCCCH
QVQAMLGVLLPGTKVAGPDAADALAVAICHANHRRR
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA