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The map label for this gene is fmt

Identifier: 103485744

GI number: 103485744

Start: 252742

End: 253662

Strand: Reverse

Name: fmt

Synonym: Sala_0249

Alternate gene names: 103485744

Gene position: 253662-252742 (Counterclockwise)

Preceding gene: 103485747

Following gene: 103485743

Centisome position: 7.58

GC content: 68.3

Gene sequence:

>921_bases
ATGCGTATCGCTTTCATGGGAACGCCGCCCTTCGCGGTGCCGACGCTCGCCGCGCTCCATACGGCGGGACATGATATTGC
GGCTGTCTATAGCCAGCCCCCGCGACCCGCGCAGCGCGGCAAGAAATTGCAGAAAAGCCCGGTGCAGCTGTGGGCCGAGG
AGCATGGCCTGCCCGTTCGGACGCCCAAAAGCCTGAAAAGCGACGAGGCGCAGGCGGAGTTTGCCGCATTGGACCTCGAT
GTCGCGGTGGTCGCGGCCTATGGGCTGATCCTGCCGCAGGCGGTGCTCGACGCACCGCGCGAAGGGTGCCTCAACGTCCA
TGGATCGATCCTGCCGCGCTGGCGCGGCGCGGCGCCGGTGCAGCGTGCGATCCTGGCGGGCGATGCCGAAACGGGCGTGA
CGATCATGCAGATGGATGCGGGGCTCGACACCGGCGCGATGCGGCTCATTGAAACGACGCCGGTCGCGCGCAAGAGCGCG
GGATTGCTGACGCACGAGCTGGCCGAAATGGGCGCGCTGATGATGCGCCGCGTGCTGAGCGAACTTCACGCCTTTCCGCC
CGTGCCGCAGCCGGACGAAGGCGTCACCTATGCGGCGAAGATCGACAAGAGCGAGGCGCGGCTCGATTTCCTGGTGAGCG
CGGTGCAGGTCGAGCGGCAGGTGCGCGCGTTCAATCCGGCGCCGGGAGCGTTTTTCGAGCTGGAGGGTGAACGCTACAAG
GTGCTGGCTGCGGAGGTCGTGCATCCTGCGGAGACGGTGGCGGGTGCGGCCCCCGGCGTCACGATCGACGATAGGCTCGC
CATCGCCTGCAACCCCGGCGCGATCCGCGTGACGCGCATCCAGCGCGCGGGGAAACCCGCGATGGAGGCGGGCGAGATGT
TGCGCGGGCGGGGAATCGCCAAGGGGACACGGCTGGCATGA

Upstream 100 bases:

>100_bases
ACCCGCTGCGACTAAGCCAGCAAGCTGGCAAGTCTCGCTGCCCTCTCCCTAAATGGGAGAGGGTTGAACTCTACGCCCGC
CTCCGCCAAAGCCCGCACCC

Downstream 100 bases:

>100_bases
GCCGCCCCTCTCCCCTTCAGGGGAGAGGATAGCGCAGCTTGCTCCGCAAGGAGCTAGCGAAGCTTGGAGAGGGGCCTGCC
CCTTCCCCCTCTCCCAACCC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MRIAFMGTPPFAVPTLAALHTAGHDIAAVYSQPPRPAQRGKKLQKSPVQLWAEEHGLPVRTPKSLKSDEAQAEFAALDLD
VAVVAAYGLILPQAVLDAPREGCLNVHGSILPRWRGAAPVQRAILAGDAETGVTIMQMDAGLDTGAMRLIETTPVARKSA
GLLTHELAEMGALMMRRVLSELHAFPPVPQPDEGVTYAAKIDKSEARLDFLVSAVQVERQVRAFNPAPGAFFELEGERYK
VLAAEVVHPAETVAGAAPGVTIDDRLAIACNPGAIRVTRIQRAGKPAMEAGEMLRGRGIAKGTRLA

Sequences:

>Translated_306_residues
MRIAFMGTPPFAVPTLAALHTAGHDIAAVYSQPPRPAQRGKKLQKSPVQLWAEEHGLPVRTPKSLKSDEAQAEFAALDLD
VAVVAAYGLILPQAVLDAPREGCLNVHGSILPRWRGAAPVQRAILAGDAETGVTIMQMDAGLDTGAMRLIETTPVARKSA
GLLTHELAEMGALMMRRVLSELHAFPPVPQPDEGVTYAAKIDKSEARLDFLVSAVQVERQVRAFNPAPGAFFELEGERYK
VLAAEVVHPAETVAGAAPGVTIDDRLAIACNPGAIRVTRIQRAGKPAMEAGEMLRGRGIAKGTRLA
>Mature_306_residues
MRIAFMGTPPFAVPTLAALHTAGHDIAAVYSQPPRPAQRGKKLQKSPVQLWAEEHGLPVRTPKSLKSDEAQAEFAALDLD
VAVVAAYGLILPQAVLDAPREGCLNVHGSILPRWRGAAPVQRAILAGDAETGVTIMQMDAGLDTGAMRLIETTPVARKSA
GLLTHELAEMGALMMRRVLSELHAFPPVPQPDEGVTYAAKIDKSEARLDFLVSAVQVERQVRAFNPAPGAFFELEGERYK
VLAAEVVHPAETVAGAAPGVTIDDRLAIACNPGAIRVTRIQRAGKPAMEAGEMLRGRGIAKGTRLA

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=311, Percent_Identity=25.08038585209, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI21614513, Length=243, Percent_Identity=27.5720164609054, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI164663775, Length=213, Percent_Identity=38.9671361502347, Blast_Score=95, Evalue=7e-20,
Organism=Escherichia coli, GI1789683, Length=314, Percent_Identity=49.6815286624204, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1788589, Length=295, Percent_Identity=27.1186440677966, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI133930964, Length=291, Percent_Identity=25.4295532646048, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45550868, Length=308, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28571984, Length=231, Percent_Identity=29.004329004329, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_SPHAL (Q1GWK0)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_615305.1
- ProteinModelPortal:   Q1GWK0
- SMR:   Q1GWK0
- GeneID:   4083107
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0249
- NMPDR:   fig|317655.9.peg.228
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- BioCyc:   SALA317655:SALA_0249-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 32573; Mature: 32573

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAFMGTPPFAVPTLAALHTAGHDIAAVYSQPPRPAQRGKKLQKSPVQLWAEEHGLPVR
CEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHCCCCCC
TPKSLKSDEAQAEFAALDLDVAVVAAYGLILPQAVLDAPREGCLNVHGSILPRWRGAAPV
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHH
QRAILAGDAETGVTIMQMDAGLDTGAMRLIETTPVARKSAGLLTHELAEMGALMMRRVLS
HHHHHCCCCCCCEEEEEECCCCCHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ELHAFPPVPQPDEGVTYAAKIDKSEARLDFLVSAVQVERQVRAFNPAPGAFFELEGERYK
HHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEE
VLAAEVVHPAETVAGAAPGVTIDDRLAIACNPGAIRVTRIQRAGKPAMEAGEMLRGRGIA
EEHHHHHCHHHHHCCCCCCCEECCCEEEEECCCCEEEEEEHHCCCCHHHHHHHHHCCCCC
KGTRLA
CCCCCC
>Mature Secondary Structure
MRIAFMGTPPFAVPTLAALHTAGHDIAAVYSQPPRPAQRGKKLQKSPVQLWAEEHGLPVR
CEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHCCCCCC
TPKSLKSDEAQAEFAALDLDVAVVAAYGLILPQAVLDAPREGCLNVHGSILPRWRGAAPV
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHH
QRAILAGDAETGVTIMQMDAGLDTGAMRLIETTPVARKSAGLLTHELAEMGALMMRRVLS
HHHHHCCCCCCCEEEEEECCCCCHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ELHAFPPVPQPDEGVTYAAKIDKSEARLDFLVSAVQVERQVRAFNPAPGAFFELEGERYK
HHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEE
VLAAEVVHPAETVAGAAPGVTIDDRLAIACNPGAIRVTRIQRAGKPAMEAGEMLRGRGIA
EEHHHHHCHHHHHCCCCCCCEECCCEEEEECCCCEEEEEEHHCCCCHHHHHHHHHCCCCC
KGTRLA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA