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The map label for this gene is truA

Identifier: 103485743

GI number: 103485743

Start: 251833

End: 252573

Strand: Reverse

Name: truA

Synonym: Sala_0248

Alternate gene names: 103485743

Gene position: 252573-251833 (Counterclockwise)

Preceding gene: 103485744

Following gene: 103485741

Centisome position: 7.55

GC content: 67.07

Gene sequence:

>741_bases
ATGACCCGCTTCGCGCTCACCATCGAATATGACGGCCGGCCCTATATGGGCTGGCAGCGGCAGGCGCACGGCCCCAGCGT
CCAGCAGGCGATCGAGGAAGCGATCCATCGCTTCACCGCCGAGGAAGTGCAGGTGCTGAGCGCCGGGCGCACCGACGCGG
GGGTCCATGCGATCGCGATGCGCGCGCATGTCGATATTGAAAAGCCGCTGACCCCGTTCAAGCTGACCGAGGCATTGAAC
GCGCAGCTCCGCCCCGCGCCGATCGCGGTCATCGGATGCGAGGTGGTGCCGGACGACTGGCACGCGCGCTTTGCCTGCAT
CGGGCGATCCTATGAATATCGCATCGTCAACCGCCGCGCGCCGCTGACGTGGGACAAGGGACTGGCGTGGCAGGTCGCGC
GCCCGCTCGACGTCGACGCGATGCACGAAGCGGCGCAGCAACTCATTGGCCTTCACGATTTCACGACCTTTCGCTCGGCG
CACTGCCAGTCGCAAAGCCCGGTCAAGACGCTCGATAGGCTGACCGTCAGCCGCCACGGCGAGGAGGTCATCATCGAGGC
CGCGGCGCGCAGCTTCCTGCACCATCAGGTGCGCTCGATGGTCGGCTCGCTCGCGCTTGTCGGCCATGGCAAATGGTCGG
CGCGCGATCTGAAGGCGGCGCTGGAGGCGGCGGACCGGCAGGCGCTGGGGCTCAATGCACCGCCCGACGGGCTGTATTTC
GTGGGGGCGGTTTATCCGTAA

Upstream 100 bases:

>100_bases
GAGGGGCCTGCCCCTTCCCCCTCTCCCAACCCTCTCCCCTGAAGGGGAGAGGGCTTACCACTCTCGACTTGGCGCCATAG
TCGCGGCTAAGGCCCCGCCC

Downstream 100 bases:

>100_bases
ACCTCCTCTCCCATCGGGAGAGGATAGCAAGCCTTGGCGGCGCAGCCGTCCAGGCGCGCTTGGTGAGGGGATCAGCCCTT
ACGAAAGCCCGCGACGTCAT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTRFALTIEYDGRPYMGWQRQAHGPSVQQAIEEAIHRFTAEEVQVLSAGRTDAGVHAIAMRAHVDIEKPLTPFKLTEALN
AQLRPAPIAVIGCEVVPDDWHARFACIGRSYEYRIVNRRAPLTWDKGLAWQVARPLDVDAMHEAAQQLIGLHDFTTFRSA
HCQSQSPVKTLDRLTVSRHGEEVIIEAAARSFLHHQVRSMVGSLALVGHGKWSARDLKAALEAADRQALGLNAPPDGLYF
VGAVYP

Sequences:

>Translated_246_residues
MTRFALTIEYDGRPYMGWQRQAHGPSVQQAIEEAIHRFTAEEVQVLSAGRTDAGVHAIAMRAHVDIEKPLTPFKLTEALN
AQLRPAPIAVIGCEVVPDDWHARFACIGRSYEYRIVNRRAPLTWDKGLAWQVARPLDVDAMHEAAQQLIGLHDFTTFRSA
HCQSQSPVKTLDRLTVSRHGEEVIIEAAARSFLHHQVRSMVGSLALVGHGKWSARDLKAALEAADRQALGLNAPPDGLYF
VGAVYP
>Mature_245_residues
TRFALTIEYDGRPYMGWQRQAHGPSVQQAIEEAIHRFTAEEVQVLSAGRTDAGVHAIAMRAHVDIEKPLTPFKLTEALNA
QLRPAPIAVIGCEVVPDDWHARFACIGRSYEYRIVNRRAPLTWDKGLAWQVARPLDVDAMHEAAQQLIGLHDFTTFRSAH
CQSQSPVKTLDRLTVSRHGEEVIIEAAARSFLHHQVRSMVGSLALVGHGKWSARDLKAALEAADRQALGLNAPPDGLYFV
GAVYP

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=276, Percent_Identity=36.231884057971, Blast_Score=131, Evalue=5e-31,
Organism=Escherichia coli, GI1788657, Length=245, Percent_Identity=41.6326530612245, Blast_Score=182, Evalue=2e-47,
Organism=Drosophila melanogaster, GI116007752, Length=302, Percent_Identity=29.4701986754967, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_SPHAL (Q1GWK1)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_615304.1
- ProteinModelPortal:   Q1GWK1
- SMR:   Q1GWK1
- GeneID:   4083106
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0248
- NMPDR:   fig|317655.9.peg.227
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   SALA317655:SALA_0248-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 27272; Mature: 27141

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFALTIEYDGRPYMGWQRQAHGPSVQQAIEEAIHRFTAEEVQVLSAGRTDAGVHAIAM
CCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
RAHVDIEKPLTPFKLTEALNAQLRPAPIAVIGCEVVPDDWHARFACIGRSYEYRIVNRRA
EEECCCCCCCCHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCEEEEECCCEEEEEECCCC
PLTWDKGLAWQVARPLDVDAMHEAAQQLIGLHDFTTFRSAHCQSQSPVKTLDRLTVSRHG
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
EEVIIEAAARSFLHHQVRSMVGSLALVGHGKWSARDLKAALEAADRQALGLNAPPDGLYF
HHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHCHHHCCCCCCCCCEEE
VGAVYP
EEEECC
>Mature Secondary Structure 
TRFALTIEYDGRPYMGWQRQAHGPSVQQAIEEAIHRFTAEEVQVLSAGRTDAGVHAIAM
CEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
RAHVDIEKPLTPFKLTEALNAQLRPAPIAVIGCEVVPDDWHARFACIGRSYEYRIVNRRA
EEECCCCCCCCHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCEEEEECCCEEEEEECCCC
PLTWDKGLAWQVARPLDVDAMHEAAQQLIGLHDFTTFRSAHCQSQSPVKTLDRLTVSRHG
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
EEVIIEAAARSFLHHQVRSMVGSLALVGHGKWSARDLKAALEAADRQALGLNAPPDGLYF
HHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHCHHHCCCCCCCCCEEE
VGAVYP
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA