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The map label for this gene is plsB [H]

Identifier: 103485690

GI number: 103485690

Start: 206601

End: 208952

Strand: Direct

Name: plsB [H]

Synonym: Sala_0194

Alternate gene names: 103485690

Gene position: 206601-208952 (Clockwise)

Preceding gene: 103485689

Following gene: 103485691

Centisome position: 6.18

GC content: 66.71

Gene sequence:

>2352_bases
GTGCCCGACGGCTCGCCCCGCACCCCGATCGTGACGGAGCAGGCGGCCGACCGGCTCTACATCATCGATGCACGTAACGG
GGTCGAACGCCGCATCCTGCTCGACTGGATTCACGCGACGTCGGACGGTGCCGGGAGCGACGACGAACCGGGCTGGGCGA
GCCTCGACATCGAGGACGGCGATCACGCGCTGCCCGTCGACGTTTTGCAGGCGCGGCTCGGCGCGACGCCCTCGCGGCTC
GTCGTTCCGCTGCGCGTCGCATGGCGCATCCCCGGCTTCGACAAGGGCCGCGCGCTCAAGTTTCGCCACCTGATCTTCGG
CGACCCGCGCCATCCGGGTTCGCTGCGCGCGCGGCTGATCCTGCTCCGCGATCGCCGCCGAGCGCAGATATTGGTGGGGG
AGGCGGCGACGCTCGACGCGCTGCGTAGCCGTTTCTGCGCACAGACCGGCGGTGGCGATGGCGAAGGCGCGGATAGCCCC
GAATTCGCACGTTTCGTCGCGCGGCAGGCGGCATTGGCGCTCGACGTCGCCGAGCGCGGCATCCGGGGAACGCGCTACAA
GGTGCCGCGCTTCGTCGCCGATGGGCTCCGCACCAGCCCCAAGTTCCGCGCGGCGCTCGCCGACCTCGCAGAGAAGACGG
GCCGCCCCGTCGCCGACCTGTTTCGCGAAGCGCGGCCGCTGATGAAGGAAGTGATCGCGCGGCCCAGCGCGCTTTTTCTC
GACCTCCGGGCCCGGCTCGATCGCATGATGTTCGGCGGCTATGCGCCGGATATGGAGATCGACGCCGCTGAACTGGCGAA
GCTGCGGGGTATCCTGCGCGAACATCCCACCTGCATCCTCTTTACGCACAAAACCTATATCGACGGCGCGACGCCAAGCC
GACTGGCCTATGAAAACGATCTGCCGATGCTGCACAGCTTCGGCGGCGCCAACCTCGACTTCGCCGTCATGGGCGAATTT
TTCCGACGGTCGGGCATGATCTTCATCCGCCGCAGCTTTCAGGACCAGCCGGTCTATAAACTCGTGCTGCGCCACTATAT
CGCCTGGCTGCTCGCCAAGCGCTTTCCGCTGAGCTGGGCGTTCGAGGGAACCCGCTCGCGGCTCGGCAAGCTGATGCCGC
CGAAGTACGGCCTGATGAAATATGTCCTCGACGCCGCCCACGCCACCGGCACGCGCGGCGTCCATTTCATCCCCTTCGTC
ACCAGTTTCGACCTCATTCGCGATGTCGAGGAATATGCCGCCGAACAGACCGGCCGCAACAAGAAGCCCGAAAGCCTGTC
GTGGTTCATCGGCTATATGAAAAGCCTGAAGGAGCCGTCGGGGCGCATCCGGCTCGACATCGGCGATCCTGTGGTCATCG
ACACCGCGCCCGGTCCCGACGACAGGCGCGCGCTCGAAAAGATCGCCTTTGCCGTCGCGGTCGAGGCGAACCGCGTCACC
CCGCTCACGGTCACCTCGGTGATGTGCCTGATCCTGCTCGGCATGGCGCCGCGCGGCGCGACGTCGGCCGAACTGCTCGG
CGCGATCGGCGCGGTGACCGGCTGGGCGCGGGCGCGCGGCATCAGGCTCAGCAGTGAACTCGAAAGCGGCGGCGATGCCG
CGCTCTCGGCGACCATCGACACGCTCGTCACCAGCGGCCTGCTCGCGCGTTATGAAGCGGGCAGCGAGACGATCTATTCG
ATCGACCCCGCCAAGCATCCGATGGCGAGCTATTACCGCAACATCATCGCGCATCATTTCCTGGGTCGCGCGATGATCGA
ACTGGCGCTGTTCGCGCTGCGCGACGCCGACAGCGGCGATGCGACCGCTGCCTTCTGGGACAGGATCGACCGATTGCGCG
ACCTGTTCAAATTCGAATTTTTCTACCCGCCGCGTGACGAGCATATGGCGTCCTTGCGCGCCGAACTGACACGCATCGAC
CCCGTGTGGGATCGCCGCCTCGCCAGCGGCGACCGCGGTATCGCGCAGCTCATCCGTCGCTGCCAGCCCGTTGTCGGCCA
CGCGATATTGCTGCCCTTTGCCGAGGCTTATTCGGTCGTAGCCGAGCTGCTCGCGCGCGCGCGGCCGGGCGATGCGGTCG
ATGAAAAAACCCTGCTCGACGCCGCGCTGGTCGAAGGCAAACAGGCCTATCTGCTGCGCCGGATCAGCAGCGAGGCGGCG
ATCGGAAAGCTGCTCTTCGCCAACGGTCTGTCGCTGATGCGGCATATGGGGCTGGCCGACGAGGCGACGCCGGAGATTAT
CGCGCAGCGCCGCGCGCTGCTGATGGAACTGCGCGGCCTTGCCAATGTCATGGAAACGATGCGCCTGTCGACGGTCGCGC
TCGCCGATCGCTTGATGGGGGCGGGGGAATGA

Upstream 100 bases:

>100_bases
CATCGAGCGAAGTCGAGATGCCTATCGGCCGGGCGCGTGCCGTCGGCGTGTCTCGACTTCGCTCGACACGAGCGGGGATG
AAGTGGAGGCTCATTAGAAG

Downstream 100 bases:

>100_bases
ATATCGCGTATCGCTCCCGCCATTCCGGCGACGGCCGGATCGTCCCCCGCTGGGGGAGGATTTTCAAGTCAGGAACCTAA
CATGCTCAAACAGCTCAGCG

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT [H]

Number of amino acids: Translated: 783; Mature: 782

Protein sequence:

>783_residues
MPDGSPRTPIVTEQAADRLYIIDARNGVERRILLDWIHATSDGAGSDDEPGWASLDIEDGDHALPVDVLQARLGATPSRL
VVPLRVAWRIPGFDKGRALKFRHLIFGDPRHPGSLRARLILLRDRRRAQILVGEAATLDALRSRFCAQTGGGDGEGADSP
EFARFVARQAALALDVAERGIRGTRYKVPRFVADGLRTSPKFRAALADLAEKTGRPVADLFREARPLMKEVIARPSALFL
DLRARLDRMMFGGYAPDMEIDAAELAKLRGILREHPTCILFTHKTYIDGATPSRLAYENDLPMLHSFGGANLDFAVMGEF
FRRSGMIFIRRSFQDQPVYKLVLRHYIAWLLAKRFPLSWAFEGTRSRLGKLMPPKYGLMKYVLDAAHATGTRGVHFIPFV
TSFDLIRDVEEYAAEQTGRNKKPESLSWFIGYMKSLKEPSGRIRLDIGDPVVIDTAPGPDDRRALEKIAFAVAVEANRVT
PLTVTSVMCLILLGMAPRGATSAELLGAIGAVTGWARARGIRLSSELESGGDAALSATIDTLVTSGLLARYEAGSETIYS
IDPAKHPMASYYRNIIAHHFLGRAMIELALFALRDADSGDATAAFWDRIDRLRDLFKFEFFYPPRDEHMASLRAELTRID
PVWDRRLASGDRGIAQLIRRCQPVVGHAILLPFAEAYSVVAELLARARPGDAVDEKTLLDAALVEGKQAYLLRRISSEAA
IGKLLFANGLSLMRHMGLADEATPEIIAQRRALLMELRGLANVMETMRLSTVALADRLMGAGE

Sequences:

>Translated_783_residues
MPDGSPRTPIVTEQAADRLYIIDARNGVERRILLDWIHATSDGAGSDDEPGWASLDIEDGDHALPVDVLQARLGATPSRL
VVPLRVAWRIPGFDKGRALKFRHLIFGDPRHPGSLRARLILLRDRRRAQILVGEAATLDALRSRFCAQTGGGDGEGADSP
EFARFVARQAALALDVAERGIRGTRYKVPRFVADGLRTSPKFRAALADLAEKTGRPVADLFREARPLMKEVIARPSALFL
DLRARLDRMMFGGYAPDMEIDAAELAKLRGILREHPTCILFTHKTYIDGATPSRLAYENDLPMLHSFGGANLDFAVMGEF
FRRSGMIFIRRSFQDQPVYKLVLRHYIAWLLAKRFPLSWAFEGTRSRLGKLMPPKYGLMKYVLDAAHATGTRGVHFIPFV
TSFDLIRDVEEYAAEQTGRNKKPESLSWFIGYMKSLKEPSGRIRLDIGDPVVIDTAPGPDDRRALEKIAFAVAVEANRVT
PLTVTSVMCLILLGMAPRGATSAELLGAIGAVTGWARARGIRLSSELESGGDAALSATIDTLVTSGLLARYEAGSETIYS
IDPAKHPMASYYRNIIAHHFLGRAMIELALFALRDADSGDATAAFWDRIDRLRDLFKFEFFYPPRDEHMASLRAELTRID
PVWDRRLASGDRGIAQLIRRCQPVVGHAILLPFAEAYSVVAELLARARPGDAVDEKTLLDAALVEGKQAYLLRRISSEAA
IGKLLFANGLSLMRHMGLADEATPEIIAQRRALLMELRGLANVMETMRLSTVALADRLMGAGE
>Mature_782_residues
PDGSPRTPIVTEQAADRLYIIDARNGVERRILLDWIHATSDGAGSDDEPGWASLDIEDGDHALPVDVLQARLGATPSRLV
VPLRVAWRIPGFDKGRALKFRHLIFGDPRHPGSLRARLILLRDRRRAQILVGEAATLDALRSRFCAQTGGGDGEGADSPE
FARFVARQAALALDVAERGIRGTRYKVPRFVADGLRTSPKFRAALADLAEKTGRPVADLFREARPLMKEVIARPSALFLD
LRARLDRMMFGGYAPDMEIDAAELAKLRGILREHPTCILFTHKTYIDGATPSRLAYENDLPMLHSFGGANLDFAVMGEFF
RRSGMIFIRRSFQDQPVYKLVLRHYIAWLLAKRFPLSWAFEGTRSRLGKLMPPKYGLMKYVLDAAHATGTRGVHFIPFVT
SFDLIRDVEEYAAEQTGRNKKPESLSWFIGYMKSLKEPSGRIRLDIGDPVVIDTAPGPDDRRALEKIAFAVAVEANRVTP
LTVTSVMCLILLGMAPRGATSAELLGAIGAVTGWARARGIRLSSELESGGDAALSATIDTLVTSGLLARYEAGSETIYSI
DPAKHPMASYYRNIIAHHFLGRAMIELALFALRDADSGDATAAFWDRIDRLRDLFKFEFFYPPRDEHMASLRAELTRIDP
VWDRRLASGDRGIAQLIRRCQPVVGHAILLPFAEAYSVVAELLARARPGDAVDEKTLLDAALVEGKQAYLLRRISSEAAI
GKLLFANGLSLMRHMGLADEATPEIIAQRRALLMELRGLANVMETMRLSTVALADRLMGAGE

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family [H]

Homologues:

Organism=Homo sapiens, GI7657134, Length=480, Percent_Identity=23.9583333333333, Blast_Score=106, Evalue=1e-22,
Organism=Homo sapiens, GI190358539, Length=305, Percent_Identity=23.9344262295082, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI87082362, Length=383, Percent_Identity=26.6318537859008, Blast_Score=106, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI25147672, Length=262, Percent_Identity=26.3358778625954, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71988723, Length=256, Percent_Identity=26.953125, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71988728, Length=182, Percent_Identity=29.6703296703297, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI17864692, Length=297, Percent_Identity=25.5892255892256, Blast_Score=100, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002123
- InterPro:   IPR022284 [H]

Pfam domain/function: PF01553 Acyltransferase [H]

EC number: =2.3.1.15 [H]

Molecular weight: Translated: 86529; Mature: 86398

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDGSPRTPIVTEQAADRLYIIDARNGVERRILLDWIHATSDGAGSDDEPGWASLDIEDG
CCCCCCCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
DHALPVDVLQARLGATPSRLVVPLRVAWRIPGFDKGRALKFRHLIFGDPRHPGSLRARLI
CCCCCHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCEEEEHHHCCCCCCCCHHHHEEE
LLRDRRRAQILVGEAATLDALRSRFCAQTGGGDGEGADSPEFARFVARQAALALDVAERG
EEECCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
IRGTRYKVPRFVADGLRTSPKFRAALADLAEKTGRPVADLFREARPLMKEVIARPSALFL
CCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHH
DLRARLDRMMFGGYAPDMEIDAAELAKLRGILREHPTCILFTHKTYIDGATPSRLAYEND
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHEECCCCCCCCCCCCC
LPMLHSFGGANLDFAVMGEFFRRSGMIFIRRSFQDQPVYKLVLRHYIAWLLAKRFPLSWA
CCHHHCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
FEGTRSRLGKLMPPKYGLMKYVLDAAHATGTRGVHFIPFVTSFDLIRDVEEYAAEQTGRN
ECCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCC
KKPESLSWFIGYMKSLKEPSGRIRLDIGDPVVIDTAPGPDDRRALEKIAFAVAVEANRVT
CCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHEEECCCCC
PLTVTSVMCLILLGMAPRGATSAELLGAIGAVTGWARARGIRLSSELESGGDAALSATID
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHCCCCCHHHHHHHH
TLVTSGLLARYEAGSETIYSIDPAKHPMASYYRNIIAHHFLGRAMIELALFALRDADSGD
HHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ATAAFWDRIDRLRDLFKFEFFYPPRDEHMASLRAELTRIDPVWDRRLASGDRGIAQLIRR
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHH
CQPVVGHAILLPFAEAYSVVAELLARARPGDAVDEKTLLDAALVEGKQAYLLRRISSEAA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
IGKLLFANGLSLMRHMGLADEATPEIIAQRRALLMELRGLANVMETMRLSTVALADRLMG
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AGE
CCC
>Mature Secondary Structure 
PDGSPRTPIVTEQAADRLYIIDARNGVERRILLDWIHATSDGAGSDDEPGWASLDIEDG
CCCCCCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
DHALPVDVLQARLGATPSRLVVPLRVAWRIPGFDKGRALKFRHLIFGDPRHPGSLRARLI
CCCCCHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCEEEEHHHCCCCCCCCHHHHEEE
LLRDRRRAQILVGEAATLDALRSRFCAQTGGGDGEGADSPEFARFVARQAALALDVAERG
EEECCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
IRGTRYKVPRFVADGLRTSPKFRAALADLAEKTGRPVADLFREARPLMKEVIARPSALFL
CCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHH
DLRARLDRMMFGGYAPDMEIDAAELAKLRGILREHPTCILFTHKTYIDGATPSRLAYEND
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHEECCCCCCCCCCCCC
LPMLHSFGGANLDFAVMGEFFRRSGMIFIRRSFQDQPVYKLVLRHYIAWLLAKRFPLSWA
CCHHHCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
FEGTRSRLGKLMPPKYGLMKYVLDAAHATGTRGVHFIPFVTSFDLIRDVEEYAAEQTGRN
ECCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCC
KKPESLSWFIGYMKSLKEPSGRIRLDIGDPVVIDTAPGPDDRRALEKIAFAVAVEANRVT
CCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHEEECCCCC
PLTVTSVMCLILLGMAPRGATSAELLGAIGAVTGWARARGIRLSSELESGGDAALSATID
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHCCCCCHHHHHHHH
TLVTSGLLARYEAGSETIYSIDPAKHPMASYYRNIIAHHFLGRAMIELALFALRDADSGD
HHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ATAAFWDRIDRLRDLFKFEFFYPPRDEHMASLRAELTRIDPVWDRRLASGDRGIAQLIRR
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHH
CQPVVGHAILLPFAEAYSVVAELLARARPGDAVDEKTLLDAALVEGKQAYLLRRISSEAA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
IGKLLFANGLSLMRHMGLADEATPEIIAQRRALLMELRGLANVMETMRLSTVALADRLMG
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AGE
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA