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The map label for this gene is ilvE [H]

Identifier: 103485545

GI number: 103485545

Start: 47553

End: 48638

Strand: Direct

Name: ilvE [H]

Synonym: Sala_0047

Alternate gene names: 103485545

Gene position: 47553-48638 (Clockwise)

Preceding gene: 103485543

Following gene: 103485546

Centisome position: 1.42

GC content: 66.21

Gene sequence:

>1086_bases
ATGTCCGCTCCCGCCTTCGCCCGTCTGCCCCATCCCAGTCCCGTGGCCGATGACGTACGCGCGGCGGCGATCGCCGATCC
CGCGTTCGGGCGGGTGTTCACCGACCATATGGTGTCGATTCGCTATACCGAAGGGCAGGGCTGGCACGACGCACAAGTCA
TGCCGCGCGGGCCGCTGACGCTCGATCCGGCGACCGCGGTGCTGCATTATGCACAGGAAATCTTCGAAGGGTTGAAGGCT
TACCGGCTCGACGATGGCTCGATGGCGCTGTTCCGCGTCGAGGCCAATGCCGCGCGCTTCAACGCCAGCGCGCGCCGCAT
GGCGATGGCCGAGCTGCCCGAGGAGTTGTTCATTGCCGCGGTAAAGGAAGCCGTCGCCGCCGACGCGCACTGGTTCCCGC
CGGTCGATGGCGGCGCGCTTTATCTGCGCCCTTTCATGTTTGCGAGCGAGGTGTTCCTGGGCGTCAAGCCCGCGTCCGAA
TATCAGTTCCTCGTCATCACCTCGCCCGTCGGCAATTATTTCAAGTCGAGCGCCCCCGCGATCTCGCTCTGGGTGTCGGA
GGATTATACCCGCGCCGCGCCGGGCGGGACGGGGGCGGCGAAATGCGGCGGCAATTATGCCGCGAGCCTGATCGCGCAGC
GCGAAGCCATCGCCAAGGGTCACGATCAGGTCGTTTTCCTCGACGCCGCCGAGCATCGCTGGATCGAGGAACTGGGCGGC
ATGAACATGTTCTTCGTCTTTGATGACGGCAGCCTCGTCACCCCGCCGCTCACCGGCACGATCCTGCCCGGCATCACCCG
CGACGCGATCATCACGCTCGCGCATGACGCGGGCCTCACGGTGCGCGAGGAAGCCTATGCGATCGACCAGTGGCAGGCCG
ATGCGGAGAGCGGCAGACTAACCGAAAGCTTTGCCTGCGGCACCGCGGCAGTGGTTACGCCGGTCGGCAAGGTGACGCGC
GGGGAGAAGAGCTTCACCATCGGCGCGGGTGGTCAAGGCCAGGTCACCGAAATGCTGAAAGCGAGGCTCGTCGATATCCA
GCGCGGCCGCGCCGCCGACCCCCATGGCTGGGTGACGCGGATCTGA

Upstream 100 bases:

>100_bases
TGAAAGAAAATTGTCATCCGGCATGGTCGCTACCGTTGCGCGCCGCGCCGGTCTTTGGCAAGAGGCGCGCTCCATAAACC
GCCAACAACAGGTGTTTTTG

Downstream 100 bases:

>100_bases
CGCGCGCCGCCTTGCCATTGATGGCGCGGACGCTATAGACGCGCTCGCACCGGCCGTTGGGGCGTAGCCAAGCGGTAAGG
CAGCGGTTTTTGGTACCGCC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSAPAFARLPHPSPVADDVRAAAIADPAFGRVFTDHMVSIRYTEGQGWHDAQVMPRGPLTLDPATAVLHYAQEIFEGLKA
YRLDDGSMALFRVEANAARFNASARRMAMAELPEELFIAAVKEAVAADAHWFPPVDGGALYLRPFMFASEVFLGVKPASE
YQFLVITSPVGNYFKSSAPAISLWVSEDYTRAAPGGTGAAKCGGNYAASLIAQREAIAKGHDQVVFLDAAEHRWIEELGG
MNMFFVFDDGSLVTPPLTGTILPGITRDAIITLAHDAGLTVREEAYAIDQWQADAESGRLTESFACGTAAVVTPVGKVTR
GEKSFTIGAGGQGQVTEMLKARLVDIQRGRAADPHGWVTRI

Sequences:

>Translated_361_residues
MSAPAFARLPHPSPVADDVRAAAIADPAFGRVFTDHMVSIRYTEGQGWHDAQVMPRGPLTLDPATAVLHYAQEIFEGLKA
YRLDDGSMALFRVEANAARFNASARRMAMAELPEELFIAAVKEAVAADAHWFPPVDGGALYLRPFMFASEVFLGVKPASE
YQFLVITSPVGNYFKSSAPAISLWVSEDYTRAAPGGTGAAKCGGNYAASLIAQREAIAKGHDQVVFLDAAEHRWIEELGG
MNMFFVFDDGSLVTPPLTGTILPGITRDAIITLAHDAGLTVREEAYAIDQWQADAESGRLTESFACGTAAVVTPVGKVTR
GEKSFTIGAGGQGQVTEMLKARLVDIQRGRAADPHGWVTRI
>Mature_360_residues
SAPAFARLPHPSPVADDVRAAAIADPAFGRVFTDHMVSIRYTEGQGWHDAQVMPRGPLTLDPATAVLHYAQEIFEGLKAY
RLDDGSMALFRVEANAARFNASARRMAMAELPEELFIAAVKEAVAADAHWFPPVDGGALYLRPFMFASEVFLGVKPASEY
QFLVITSPVGNYFKSSAPAISLWVSEDYTRAAPGGTGAAKCGGNYAASLIAQREAIAKGHDQVVFLDAAEHRWIEELGGM
NMFFVFDDGSLVTPPLTGTILPGITRDAIITLAHDAGLTVREEAYAIDQWQADAESGRLTESFACGTAAVVTPVGKVTRG
EKSFTIGAGGQGQVTEMLKARLVDIQRGRAADPHGWVTRI

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI38176287, Length=330, Percent_Identity=39.0909090909091, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI296010904, Length=330, Percent_Identity=39.0909090909091, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI296010906, Length=330, Percent_Identity=39.0909090909091, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI50658084, Length=338, Percent_Identity=34.3195266272189, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI296010902, Length=330, Percent_Identity=34.8484848484849, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI296010900, Length=330, Percent_Identity=34.8484848484849, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI258614015, Length=293, Percent_Identity=33.4470989761092, Blast_Score=162, Evalue=3e-40,
Organism=Escherichia coli, GI48994963, Length=322, Percent_Identity=33.2298136645963, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17568601, Length=301, Percent_Identity=38.5382059800665, Blast_Score=211, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI17565728, Length=342, Percent_Identity=35.3801169590643, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6322002, Length=361, Percent_Identity=38.2271468144044, Blast_Score=235, Evalue=8e-63,
Organism=Saccharomyces cerevisiae, GI6322608, Length=305, Percent_Identity=37.7049180327869, Blast_Score=214, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24641779, Length=334, Percent_Identity=40.7185628742515, Blast_Score=238, Evalue=7e-63,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 38770; Mature: 38639

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPAFARLPHPSPVADDVRAAAIADPAFGRVFTDHMVSIRYTEGQGWHDAQVMPRGPLT
CCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCEEEEEEECCCCCCCCEECCCCCCE
LDPATAVLHYAQEIFEGLKAYRLDDGSMALFRVEANAARFNASARRMAMAELPEELFIAA
ECHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCHHHCCHHHHHHHHHHCCHHHHHHH
VKEAVAADAHWFPPVDGGALYLRPFMFASEVFLGVKPASEYQFLVITSPVGNYFKSSAPA
HHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHEECCCCCCEEEEEEECCCCHHHHCCCCE
ISLWVSEDYTRAAPGGTGAAKCGGNYAASLIAQREAIAKGHDQVVFLDAAEHRWIEELGG
EEEEEECCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHCC
MNMFFVFDDGSLVTPPLTGTILPGITRDAIITLAHDAGLTVREEAYAIDQWQADAESGRL
CEEEEEECCCCEECCCCCCCCCCCCCCCEEEEEEECCCCEEEHHHHHHHHHCCCCCCCCC
TESFACGTAAVVTPVGKVTRGEKSFTIGAGGQGQVTEMLKARLVDIQRGRAADPHGWVTR
CHHHCCCCCHHHCCCHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEC
I
C
>Mature Secondary Structure 
SAPAFARLPHPSPVADDVRAAAIADPAFGRVFTDHMVSIRYTEGQGWHDAQVMPRGPLT
CCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCEEEEEEECCCCCCCCEECCCCCCE
LDPATAVLHYAQEIFEGLKAYRLDDGSMALFRVEANAARFNASARRMAMAELPEELFIAA
ECHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCHHHCCHHHHHHHHHHCCHHHHHHH
VKEAVAADAHWFPPVDGGALYLRPFMFASEVFLGVKPASEYQFLVITSPVGNYFKSSAPA
HHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHEECCCCCCEEEEEEECCCCHHHHCCCCE
ISLWVSEDYTRAAPGGTGAAKCGGNYAASLIAQREAIAKGHDQVVFLDAAEHRWIEELGG
EEEEEECCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHCC
MNMFFVFDDGSLVTPPLTGTILPGITRDAIITLAHDAGLTVREEAYAIDQWQADAESGRL
CEEEEEECCCCEECCCCCCCCCCCCCCCEEEEEEECCCCEEEHHHHHHHHHCCCCCCCCC
TESFACGTAAVVTPVGKVTRGEKSFTIGAGGQGQVTEMLKARLVDIQRGRAADPHGWVTR
CHHHCCCCCHHHCCCHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEC
I
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]