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The map label for this gene is metH [H]

Identifier: 103485535

GI number: 103485535

Start: 36101

End: 38722

Strand: Direct

Name: metH [H]

Synonym: Sala_0037

Alternate gene names: 103485535

Gene position: 36101-38722 (Clockwise)

Preceding gene: 103485534

Following gene: 103485536

Centisome position: 1.08

GC content: 62.47

Gene sequence:

>2622_bases
ATGACCGAAACCCTTTCCTCCTCCACCTTCGTCAACATCGGTGAACGCACCAACGTCACCGGTTCAGCGGCGTTCAAGAG
GCTGATCCTCGCCGACGACTATGCCGCGGCGGTCGAGGTCGCGCGCCAGCAGGTCGAAAATGGCGCGCAGATCATCGACG
TCAATATGGACGAAGGCCTACTCGACGCCGAACATGCGATGACGACCTTCCTCAAGCTCATCGCCGCCGAGCCCGACATT
GCGCGCGTGCCGGTGATGATCGACAGTTCGAAATGGAGCGTGATCGAGGCCGGGCTGAAGTGCGTTCCCGGCAAGCCGAT
CGTCAATTCGATCAGCATGAAGGAAGGCGAGGAGCAATTCCTGGACCATGCGAAGAAGTGCATGGCCTATGGCGCCGCGG
TCGTCGTCATGGCGTTCGACGAGGTCGGACAGGCCGACACCAGGGAGCGCAAGGTCGAGATTTGCGAGCGTGCCTACAAG
CTGCTGATGACCATCGGCTTTCCGCCCGAGGACATTATCTTTGATCCCAATATCTTCGCGGTCGCGACGGGGATCGAGGA
GCATGACAATTATGCGGTCGATTTCATCGAGGCGGTGAAGGAGATCCGCGTCCGCTGTCCGCACGCGCATTTTTCGGGGG
GGCTGTCGAACCTGTCGTTCAGCTTTCGCGGCAACGAGACCGTGCGCCGCGCGATGCACAGCGTCTTTCTCTACCATGCA
ATTCCCGCGGGACTCGACATGGCGATCGTCAACGCCGGGCAGCTCGACGTCTATGACACGATCGACCCCGAACTCAGGAC
CGCGTGCGAGGATGTCATCCTCAACCGCAAGGTCGAAGGCGAAGAGCTCAGCCCTACCGAACGGCTGATTGCGCTCGCCG
AACGCTATAAGGGAACGAACGCCGCGCAGGAAAAGGCGGCTGAGGAATGGCGCGGCTGGGCGGTCGAAAAGCGCCTTGAA
CATGCGCTGGTCAAGGGCATCGACGCGCATATCGTCGAAGATACCGAAGAAATGCGCCGCGCCATGGATCGTCCGATCGA
GGTGATCGAAGGGCCGCTGATGGACGGGATGAACGTCGTCGGCGACCTGTTCGGGTCGGGCAAAATGTTCCTGCCGCAGG
TCGTCAAATCGGCGCGCGTGATGAAAAAGGCGGTCGCGCACCTGCTGCCTTTCATCGAGGCGTCGAAGGAGCCGGGAGCA
AAGGGCAAGGGCAAGATCGTGATGGCGACCGTCAAGGGCGACGTCCACGACATCGGCAAGAATATCGTCGGCGTCGTGCT
GCAGTGCAACGGTTTCGAAATCATCGACCTGGGCGTGATGGTGCCGTGGAGCAAGATCCTGGAGGCCGCGAACGAGAATG
ACGCCGACATGATCGGTCTGTCGGGCCTCATCACCCCCTCACTCGACGAGATGGTGACGGTGGCCGAGGAAATGCAGCGC
GCGGGCATGACGATTCCGCTCCTCATCGGCGGCGCGACGACGTCGAAGGTCCATACCGCGCTGCGCATCGACCCGGCCTA
TCAGGGGCCGGTGCTCCACGTTCTCGACGCCAGCCGCGCGGTCGGCGTCGCGACCGCGCTGGTCAGCGACACCGGGCGCG
ATGCCTATGTGAAGGGGTATAAGAATGATTACGCCCATGTCCGCGACGTGCGCGCGGGCAAGGGGCAAAGCGTGCTGCTG
AGCATCGAGGAGGCGCGCGCCAATTATTACGATGCCTATTTGAGCGACAAGCCCGCACCGCCGCTCCAGCCCGGCCTCCA
CCGCTTCGACGACTGGTCGCTCGCGGACCTGCGCGACTATATCGACTGGACGCCCTTCTTTCGCGCATGGGAACTGCATG
GAACCTGGCCGTCGATCCTGCAAGACGAGGTGGTCGGGGAGACGGCGCGGGCGCTCAAGGCCGATGCCGATGCGATGCTC
GACCAGATTATTGCGGAGCAATGGCTGACGGCGCGTGGGGTTTGCGCCTTCTGGCCGTGTGCGCGGCACGGTGACGATAT
TGTCATCCACCTTGCCGAGGAAGAGCGCCATGTGACGCTCCCGTTCCTGCGCCAGCAAATCAAGAAGAGCCGCGACCGCG
CCAATATGTGTCTCGCCGACTTCATCGACCCGGCGGGTGACTGGATCGGCGGCTTTGCGGTCGGCATCCATGGGATCGAG
CCGCATTCGGAGCGCTTCCGCGCCGACAAGGATGATTATTCGGACATTTTGCTCAAAGCGCTCGCCGACCGCTTCGCCGA
AGCCTTTGCCGAGCGGCTGCACCAGCATGTCCGCACCACATTATGGGGCTATGCGCCGGGCGAACAGCTCACCAACGAAG
CGCTGATCAAGGAGGAATATCGCGGCATCCGCCCGGCGCCCGGTTACCCGGCGTGCCCCGACCACAGCCTGAAACCGATC
CTGTTCGATCTGCTTGGCGCGGGAGAGAATGCCGGGCTGGTGCTGACCGAAAGTTTTGCGATGCTGCCGACCGCGGCGGT
GAGCGGCTTTTATTTCGGCCATCCCGAAAGCCAGTATTTCGGCGTAGCGCGGATCGGCAGTGACCAGCTGGAGGATTATG
CCCGGCGACGCGGCGTCGATCTGGAAACCGCGACGCGCTGGCTGCGGCCCAATCTGGATTGA

Upstream 100 bases:

>100_bases
GGTTAGGGGAGGGGCTGTCCCTGTCCGACGGCGCCGCCCGAGATTTGAAACAGACCCTCCCCCGACCCCTCCCGCAAGCG
GGAGGGGAGAAGAGAGCCCG

Downstream 100 bases:

>100_bases
GGCGCTCTAGCCATTAATTCCGATCGGCGCGCCTGTCCGAAAGCGAGACAGGCGTGCCGTTAACCAGCTTCGCGAACGAC
CGCCGTGCCGAAGGGCGCTA

Product: 5-methyltetrahydrofolate--homocysteine methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS [H]

Number of amino acids: Translated: 873; Mature: 872

Protein sequence:

>873_residues
MTETLSSSTFVNIGERTNVTGSAAFKRLILADDYAAAVEVARQQVENGAQIIDVNMDEGLLDAEHAMTTFLKLIAAEPDI
ARVPVMIDSSKWSVIEAGLKCVPGKPIVNSISMKEGEEQFLDHAKKCMAYGAAVVVMAFDEVGQADTRERKVEICERAYK
LLMTIGFPPEDIIFDPNIFAVATGIEEHDNYAVDFIEAVKEIRVRCPHAHFSGGLSNLSFSFRGNETVRRAMHSVFLYHA
IPAGLDMAIVNAGQLDVYDTIDPELRTACEDVILNRKVEGEELSPTERLIALAERYKGTNAAQEKAAEEWRGWAVEKRLE
HALVKGIDAHIVEDTEEMRRAMDRPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAHLLPFIEASKEPGA
KGKGKIVMATVKGDVHDIGKNIVGVVLQCNGFEIIDLGVMVPWSKILEAANENDADMIGLSGLITPSLDEMVTVAEEMQR
AGMTIPLLIGGATTSKVHTALRIDPAYQGPVLHVLDASRAVGVATALVSDTGRDAYVKGYKNDYAHVRDVRAGKGQSVLL
SIEEARANYYDAYLSDKPAPPLQPGLHRFDDWSLADLRDYIDWTPFFRAWELHGTWPSILQDEVVGETARALKADADAML
DQIIAEQWLTARGVCAFWPCARHGDDIVIHLAEEERHVTLPFLRQQIKKSRDRANMCLADFIDPAGDWIGGFAVGIHGIE
PHSERFRADKDDYSDILLKALADRFAEAFAERLHQHVRTTLWGYAPGEQLTNEALIKEEYRGIRPAPGYPACPDHSLKPI
LFDLLGAGENAGLVLTESFAMLPTAAVSGFYFGHPESQYFGVARIGSDQLEDYARRRGVDLETATRWLRPNLD

Sequences:

>Translated_873_residues
MTETLSSSTFVNIGERTNVTGSAAFKRLILADDYAAAVEVARQQVENGAQIIDVNMDEGLLDAEHAMTTFLKLIAAEPDI
ARVPVMIDSSKWSVIEAGLKCVPGKPIVNSISMKEGEEQFLDHAKKCMAYGAAVVVMAFDEVGQADTRERKVEICERAYK
LLMTIGFPPEDIIFDPNIFAVATGIEEHDNYAVDFIEAVKEIRVRCPHAHFSGGLSNLSFSFRGNETVRRAMHSVFLYHA
IPAGLDMAIVNAGQLDVYDTIDPELRTACEDVILNRKVEGEELSPTERLIALAERYKGTNAAQEKAAEEWRGWAVEKRLE
HALVKGIDAHIVEDTEEMRRAMDRPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAHLLPFIEASKEPGA
KGKGKIVMATVKGDVHDIGKNIVGVVLQCNGFEIIDLGVMVPWSKILEAANENDADMIGLSGLITPSLDEMVTVAEEMQR
AGMTIPLLIGGATTSKVHTALRIDPAYQGPVLHVLDASRAVGVATALVSDTGRDAYVKGYKNDYAHVRDVRAGKGQSVLL
SIEEARANYYDAYLSDKPAPPLQPGLHRFDDWSLADLRDYIDWTPFFRAWELHGTWPSILQDEVVGETARALKADADAML
DQIIAEQWLTARGVCAFWPCARHGDDIVIHLAEEERHVTLPFLRQQIKKSRDRANMCLADFIDPAGDWIGGFAVGIHGIE
PHSERFRADKDDYSDILLKALADRFAEAFAERLHQHVRTTLWGYAPGEQLTNEALIKEEYRGIRPAPGYPACPDHSLKPI
LFDLLGAGENAGLVLTESFAMLPTAAVSGFYFGHPESQYFGVARIGSDQLEDYARRRGVDLETATRWLRPNLD
>Mature_872_residues
TETLSSSTFVNIGERTNVTGSAAFKRLILADDYAAAVEVARQQVENGAQIIDVNMDEGLLDAEHAMTTFLKLIAAEPDIA
RVPVMIDSSKWSVIEAGLKCVPGKPIVNSISMKEGEEQFLDHAKKCMAYGAAVVVMAFDEVGQADTRERKVEICERAYKL
LMTIGFPPEDIIFDPNIFAVATGIEEHDNYAVDFIEAVKEIRVRCPHAHFSGGLSNLSFSFRGNETVRRAMHSVFLYHAI
PAGLDMAIVNAGQLDVYDTIDPELRTACEDVILNRKVEGEELSPTERLIALAERYKGTNAAQEKAAEEWRGWAVEKRLEH
ALVKGIDAHIVEDTEEMRRAMDRPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAHLLPFIEASKEPGAK
GKGKIVMATVKGDVHDIGKNIVGVVLQCNGFEIIDLGVMVPWSKILEAANENDADMIGLSGLITPSLDEMVTVAEEMQRA
GMTIPLLIGGATTSKVHTALRIDPAYQGPVLHVLDASRAVGVATALVSDTGRDAYVKGYKNDYAHVRDVRAGKGQSVLLS
IEEARANYYDAYLSDKPAPPLQPGLHRFDDWSLADLRDYIDWTPFFRAWELHGTWPSILQDEVVGETARALKADADAMLD
QIIAEQWLTARGVCAFWPCARHGDDIVIHLAEEERHVTLPFLRQQIKKSRDRANMCLADFIDPAGDWIGGFAVGIHGIEP
HSERFRADKDDYSDILLKALADRFAEAFAERLHQHVRTTLWGYAPGEQLTNEALIKEEYRGIRPAPGYPACPDHSLKPIL
FDLLGAGENAGLVLTESFAMLPTAAVSGFYFGHPESQYFGVARIGSDQLEDYARRRGVDLETATRWLRPNLD

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=900, Percent_Identity=52.2222222222222, Blast_Score=882, Evalue=0.0,
Organism=Escherichia coli, GI1790450, Length=882, Percent_Identity=61.6780045351474, Blast_Score=1045, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17535405, Length=896, Percent_Identity=50.78125, Blast_Score=842, Evalue=0.0,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 96333; Mature: 96202

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50974 ADOMET_ACTIVATION ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETLSSSTFVNIGERTNVTGSAAFKRLILADDYAAAVEVARQQVENGAQIIDVNMDEGL
CCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCC
LDAEHAMTTFLKLIAAEPDIARVPVMIDSSKWSVIEAGLKCVPGKPIVNSISMKEGEEQF
CHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHCCCCCCCCCCHHCCCCCCCHHHH
LDHAKKCMAYGAAVVVMAFDEVGQADTRERKVEICERAYKLLMTIGFPPEDIIFDPNIFA
HHHHHHHHHHCHHEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCEEE
VATGIEEHDNYAVDFIEAVKEIRVRCPHAHFSGGLSNLSFSFRGNETVRRAMHSVFLYHA
EEECCHHCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHH
IPAGLDMAIVNAGQLDVYDTIDPELRTACEDVILNRKVEGEELSPTERLIALAERYKGTN
HCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
AAQEKAAEEWRGWAVEKRLEHALVKGIDAHIVEDTEEMRRAMDRPIEVIEGPLMDGMNVV
HHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCHHHHCCCHHHHHHHH
GDLFGSGKMFLPQVVKSARVMKKAVAHLLPFIEASKEPGAKGKGKIVMATVKGDVHDIGK
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHH
NIVGVVLQCNGFEIIDLGVMVPWSKILEAANENDADMIGLSGLITPSLDEMVTVAEEMQR
HHEEEEEEECCEEEEEECEECCHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHH
AGMTIPLLIGGATTSKVHTALRIDPAYQGPVLHVLDASRAVGVATALVSDTGRDAYVKGY
CCCEEEEEEECCCHHHHEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEECCC
KNDYAHVRDVRAGKGQSVLLSIEEARANYYDAYLSDKPAPPLQPGLHRFDDWSLADLRDY
CCCHHHHHHHCCCCCCEEEEEEHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
IDWTPFFRAWELHGTWPSILQDEVVGETARALKADADAMLDQIIAEQWLTARGVCAFWPC
HCCCHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCC
ARHGDDIVIHLAEEERHVTLPFLRQQIKKSRDRANMCLADFIDPAGDWIGGFAVGIHGIE
CCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHEEECCCC
PHSERFRADKDDYSDILLKALADRFAEAFAERLHQHVRTTLWGYAPGEQLTNEALIKEEY
CCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RGIRPAPGYPACPDHSLKPILFDLLGAGENAGLVLTESFAMLPTAAVSGFYFGHPESQYF
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCEE
GVARIGSDQLEDYARRRGVDLETATRWLRPNLD
EEEECCHHHHHHHHHHCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TETLSSSTFVNIGERTNVTGSAAFKRLILADDYAAAVEVARQQVENGAQIIDVNMDEGL
CCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCC
LDAEHAMTTFLKLIAAEPDIARVPVMIDSSKWSVIEAGLKCVPGKPIVNSISMKEGEEQF
CHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHCCCCCCCCCCHHCCCCCCCHHHH
LDHAKKCMAYGAAVVVMAFDEVGQADTRERKVEICERAYKLLMTIGFPPEDIIFDPNIFA
HHHHHHHHHHCHHEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCEEE
VATGIEEHDNYAVDFIEAVKEIRVRCPHAHFSGGLSNLSFSFRGNETVRRAMHSVFLYHA
EEECCHHCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHH
IPAGLDMAIVNAGQLDVYDTIDPELRTACEDVILNRKVEGEELSPTERLIALAERYKGTN
HCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
AAQEKAAEEWRGWAVEKRLEHALVKGIDAHIVEDTEEMRRAMDRPIEVIEGPLMDGMNVV
HHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCHHHHCCCHHHHHHHH
GDLFGSGKMFLPQVVKSARVMKKAVAHLLPFIEASKEPGAKGKGKIVMATVKGDVHDIGK
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHH
NIVGVVLQCNGFEIIDLGVMVPWSKILEAANENDADMIGLSGLITPSLDEMVTVAEEMQR
HHEEEEEEECCEEEEEECEECCHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHH
AGMTIPLLIGGATTSKVHTALRIDPAYQGPVLHVLDASRAVGVATALVSDTGRDAYVKGY
CCCEEEEEEECCCHHHHEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEECCC
KNDYAHVRDVRAGKGQSVLLSIEEARANYYDAYLSDKPAPPLQPGLHRFDDWSLADLRDY
CCCHHHHHHHCCCCCCEEEEEEHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
IDWTPFFRAWELHGTWPSILQDEVVGETARALKADADAMLDQIIAEQWLTARGVCAFWPC
HCCCHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCC
ARHGDDIVIHLAEEERHVTLPFLRQQIKKSRDRANMCLADFIDPAGDWIGGFAVGIHGIE
CCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHEEECCCC
PHSERFRADKDDYSDILLKALADRFAEAFAERLHQHVRTTLWGYAPGEQLTNEALIKEEY
CCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RGIRPAPGYPACPDHSLKPILFDLLGAGENAGLVLTESFAMLPTAAVSGFYFGHPESQYF
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCEE
GVARIGSDQLEDYARRRGVDLETATRWLRPNLD
EEEECCHHHHHHHHHHCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2185137; 2668277; 8369296; 8265357; 9278503; 1593636; 7992050; 8939751 [H]