BacMap

Definition	Ruegeria sp. TM1040, complete genome.
Accession	NC_008044
Length	3,200,938

Click here to switch to the map view.

The map label for this gene is fadB1 [H]

Identifier: 99082870

GI number: 99082870

Start: 3199021

End: 3199797

Strand: Direct

Name: fadB1 [H]

Synonym: TM1040_3030

Alternate gene names: 99082870

Gene position: 3199021-3199797 (Clockwise)

Preceding gene: 99082869

Following gene: 99082871

Centisome position: 99.94

GC content: 57.01

Gene sequence:

>777_bases
ATGGCCTATGAGACGATCAACGTCGATACTCAGGACCACGTCTGCCTTATCAAGCTGAACCGGCCTGAAGCGTTGAACGC
GCTCAATTCCGTCCTTTTGGGCGAGTTGTGTGCCGCTTTGGAAGAAGCGGACGCCAGCGACAAAGTGCGCTGCATCGTGC
TGACCGGATCGGAAAAGGCATTTGCAGCGGGCGCCGACATCAAGGAGATGTCGGAGATGAGCTATGTGGATGTGTTCACA
TCCAACCTCTTTGCGGACGCCAATGACCGGATCGCTGCCATCCGCAAACCGATCATCGCTGCGGTGTCTGGCTATGCGCT
GGGCGGCGGGTGCGAAGTGGCCATGATGTGTGATTTCATCATCGCGGCTGAGACCGCGAAATTCGGCCAGCCCGAGATCA
ATCTGGGTGTCATTGCCGGTATGGGCGGCACGCAACGCCTCACCCGTTTTGTGGGCAAGTCGAAGTCGATGGACATGAAT
CTGACCGGCCGCTTCATGACTGCCGATGAGGCAGAGCGGGCAGGTCTGGTGTCGCGCGTGGTTCCGGCCAAAAAGCTGAT
CGAAGAGGCGATGGCAGCAGCCTCTAAGATCGCCGAGAAATCAGTACTGACCTCGATTGCGGCCAAGGAGGCCGTCAATC
GCGCGTATGAATTGCCGCTTAGCGAAGGCATGCTCTTTGAGCGGCGGGTCTTCCATTCCATGTTTGCGACGGAAGACCAA
AAGGAAGGTATGGCAGCGTTTCTAGAAAAACGCGAAGCCCAGTTCCGAGACAAGTAA

Upstream 100 bases:

>100_bases
GGTCGTCGTTCTACTGTGGCAAGTGCCAAAGATAACTTGAAAGCGAGGGCTGCCGTGATAAGGACTCAGGCTCAGGAGAA
ACAACCGAAAGCGAATTGTC

Downstream 100 bases:

>100_bases
CCAGATTTGGCGAGGGGCGGCGGAATTTTGTCTCTCGCACATAGATAGTTCTACTCCGGCGATCCTGCCAATAATAACAG
TGCCTTACAGCCTTTCAGAA

Product: short chain enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MAYETINVDTQDHVCLIKLNRPEALNALNSVLLGELCAALEEADASDKVRCIVLTGSEKAFAAGADIKEMSEMSYVDVFT
SNLFADANDRIAAIRKPIIAAVSGYALGGGCEVAMMCDFIIAAETAKFGQPEINLGVIAGMGGTQRLTRFVGKSKSMDMN
LTGRFMTADEAERAGLVSRVVPAKKLIEEAMAAASKIAEKSVLTSIAAKEAVNRAYELPLSEGMLFERRVFHSMFATEDQ
KEGMAAFLEKREAQFRDK

Sequences:

>Translated_258_residues
MAYETINVDTQDHVCLIKLNRPEALNALNSVLLGELCAALEEADASDKVRCIVLTGSEKAFAAGADIKEMSEMSYVDVFT
SNLFADANDRIAAIRKPIIAAVSGYALGGGCEVAMMCDFIIAAETAKFGQPEINLGVIAGMGGTQRLTRFVGKSKSMDMN
LTGRFMTADEAERAGLVSRVVPAKKLIEEAMAAASKIAEKSVLTSIAAKEAVNRAYELPLSEGMLFERRVFHSMFATEDQ
KEGMAAFLEKREAQFRDK
>Mature_257_residues
AYETINVDTQDHVCLIKLNRPEALNALNSVLLGELCAALEEADASDKVRCIVLTGSEKAFAAGADIKEMSEMSYVDVFTS
NLFADANDRIAAIRKPIIAAVSGYALGGGCEVAMMCDFIIAAETAKFGQPEINLGVIAGMGGTQRLTRFVGKSKSMDMNL
TGRFMTADEAERAGLVSRVVPAKKLIEEAMAAASKIAEKSVLTSIAAKEAVNRAYELPLSEGMLFERRVFHSMFATEDQK
EGMAAFLEKREAQFRDK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=247, Percent_Identity=54.251012145749, Blast_Score=265, Evalue=4e-71,
Organism=Homo sapiens, GI68989263, Length=234, Percent_Identity=31.6239316239316, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI20127408, Length=195, Percent_Identity=37.9487179487179, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=29.6992481203008, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI4502327, Length=250, Percent_Identity=34.4, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI31542718, Length=243, Percent_Identity=30.0411522633745, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI150378541, Length=249, Percent_Identity=30.9236947791165, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI37594471, Length=219, Percent_Identity=29.2237442922374, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI37594469, Length=219, Percent_Identity=29.2237442922374, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI157694516, Length=248, Percent_Identity=24.1935483870968, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI213417737, Length=248, Percent_Identity=24.1935483870968, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4757968, Length=261, Percent_Identity=25.2873563218391, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI49355787, Length=261, Percent_Identity=25.2873563218391, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI25453481, Length=267, Percent_Identity=25.0936329588015, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI51538573, Length=267, Percent_Identity=25.0936329588015, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI221136756, Length=264, Percent_Identity=25.7575757575758, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221136753, Length=264, Percent_Identity=25.7575757575758, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI45643119, Length=200, Percent_Identity=26.5, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260275230, Length=200, Percent_Identity=26.5, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260274832, Length=199, Percent_Identity=26.6331658291457, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI221307494, Length=264, Percent_Identity=25.7575757575758, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI261878539, Length=139, Percent_Identity=33.0935251798561, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4757966, Length=256, Percent_Identity=25.390625, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI51538581, Length=256, Percent_Identity=25.390625, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=46.1240310077519, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=33.8403041825095, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=33.4615384615385, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788597, Length=258, Percent_Identity=32.5581395348837, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787660, Length=250, Percent_Identity=31.6, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790281, Length=198, Percent_Identity=31.3131313131313, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1788682, Length=233, Percent_Identity=32.1888412017167, Blast_Score=98, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=53.7848605577689, Blast_Score=269, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=49.1803278688525, Blast_Score=241, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI17540714, Length=221, Percent_Identity=31.2217194570136, Blast_Score=116, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17560910, Length=243, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17558304, Length=186, Percent_Identity=34.4086021505376, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17536985, Length=262, Percent_Identity=29.3893129770992, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17540306, Length=233, Percent_Identity=31.3304721030043, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=261, Percent_Identity=28.3524904214559, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI25144276, Length=184, Percent_Identity=34.2391304347826, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=184, Percent_Identity=34.2391304347826, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17508951, Length=184, Percent_Identity=34.2391304347826, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=208, Percent_Identity=30.7692307692308, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI25144160, Length=209, Percent_Identity=30.622009569378, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=209, Percent_Identity=30.622009569378, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320241, Length=188, Percent_Identity=31.3829787234043, Blast_Score=89, Evalue=7e-19,
Organism=Drosophila melanogaster, GI20129971, Length=246, Percent_Identity=57.3170731707317, Blast_Score=272, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24653477, Length=246, Percent_Identity=57.3170731707317, Blast_Score=272, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=33.4645669291339, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI19922422, Length=246, Percent_Identity=29.6747967479675, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21357171, Length=258, Percent_Identity=30.2325581395349, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24650670, Length=221, Percent_Identity=31.2217194570136, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19920382, Length=267, Percent_Identity=28.0898876404494, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24654903, Length=212, Percent_Identity=27.8301886792453, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI19921000, Length=191, Percent_Identity=33.5078534031414, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24583077, Length=191, Percent_Identity=33.5078534031414, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24583079, Length=191, Percent_Identity=33.5078534031414, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571729, Length=174, Percent_Identity=28.1609195402299, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI28571730, Length=174, Percent_Identity=28.1609195402299, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24583165, Length=176, Percent_Identity=27.8409090909091, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27908; Mature: 27777

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYETINVDTQDHVCLIKLNRPEALNALNSVLLGELCAALEEADASDKVRCIVLTGSEKA
CCEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
FAAGADIKEMSEMSYVDVFTSNLFADANDRIAAIRKPIIAAVSGYALGGGCEVAMMCDFI
EECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEECCCCHHHHHHHHH
IAAETAKFGQPEINLGVIAGMGGTQRLTRFVGKSKSMDMNLTGRFMTADEAERAGLVSRV
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHH
VPAKKLIEEAMAAASKIAEKSVLTSIAAKEAVNRAYELPLSEGMLFERRVFHSMFATEDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCH
KEGMAAFLEKREAQFRDK
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AYETINVDTQDHVCLIKLNRPEALNALNSVLLGELCAALEEADASDKVRCIVLTGSEKA
CEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
FAAGADIKEMSEMSYVDVFTSNLFADANDRIAAIRKPIIAAVSGYALGGGCEVAMMCDFI
EECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEECCCCHHHHHHHHH
IAAETAKFGQPEINLGVIAGMGGTQRLTRFVGKSKSMDMNLTGRFMTADEAERAGLVSRV
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHH
VPAKKLIEEAMAAASKIAEKSVLTSIAAKEAVNRAYELPLSEGMLFERRVFHSMFATEDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCH
KEGMAAFLEKREAQFRDK
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]