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The map label for this gene is mcbR [H]

Identifier: 99082825

GI number: 99082825

Start: 3149540

End: 3150286

Strand: Direct

Name: mcbR [H]

Synonym: TM1040_2985

Alternate gene names: 99082825

Gene position: 3149540-3150286 (Clockwise)

Preceding gene: 99082819

Following gene: 99082826

Centisome position: 98.39

GC content: 57.83

Gene sequence:

>747_bases
ATGTTTTCATCTGCATTCTGTTGGTGCAAGCTGCCCAAAAATGAGAAGGATCCAAGCCCGGTGAATGTGCCAAATACGCC
ATCTGTTACCCCCGCGGAGGGGGGGCAAAAACTACCTGCTCACGAGCTGGTCTATCAACGTCTTCGGGCGCAGATTTTGT
TTGGTGAGCTGGCGCCGGGCCAGGCGGTCACCATACAGGGTCTGGTCGAGGCATTGGACAGTGGCATGACCCCAGTGCGC
GAAGCGATCCGCAGGCTGATCTCGGACGGGGCGCTGGTGTTCCAAGGGAACCGTCGTGTTTCTGTCCCCGAGCTTCGTGC
CGAGGATGTTGATGAGTTAATTTACGCAAGGAAAACAATGGAGTGTGAGCTTGCCCGTCGCGCAGCCACACGGATTCCCT
CCGCCAGTGTCGATGCGCTTGAGGCGCTTGATCTGCGCTTGGATACGGCCATCGCAAATGGCGATGTCGCAGGGTACCTG
GGTCTCAATTACGAATTCCACAGCATGCTGAACGAATACGCTGATGCCCCCATCCTCGCGGATCTGGCTGATCGTCTTTG
GCTGCGCTTTGGTCCGTCCCTGCGCGTGGTCTGCGGGCGCTTCGGAACCCAGAGCTTTCCTGACCGCCACAAAGACATCC
TTGAAGGGCTGCGTAAAAAAGATGCAGATCTTGTCTCTTTGGCGATGGAGCGGGACGTGTTGCAGGGCATGGAGCAGGTT
CGGGCGGGGCTCACCCCGATCAACTGA

Upstream 100 bases:

>100_bases
GTTTTGGTTTTCATGAGAATCTCCCCTGTGCATGCCGGAGTCTTCCTGTCCGGCTATGGGCTATTTTGATCAAGTTTTGC
CTCATCCGGCAAGACACTTT

Downstream 100 bases:

>100_bases
TTCGATTGACAGGCGATTTTTTGATCATATTCTGAGCGAAAGATTTCCGTTCAGGAGTGACTCATGACGACGATTACCAA
CCATCTGCCAACCGCAGAAC

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MFSSAFCWCKLPKNEKDPSPVNVPNTPSVTPAEGGQKLPAHELVYQRLRAQILFGELAPGQAVTIQGLVEALDSGMTPVR
EAIRRLISDGALVFQGNRRVSVPELRAEDVDELIYARKTMECELARRAATRIPSASVDALEALDLRLDTAIANGDVAGYL
GLNYEFHSMLNEYADAPILADLADRLWLRFGPSLRVVCGRFGTQSFPDRHKDILEGLRKKDADLVSLAMERDVLQGMEQV
RAGLTPIN

Sequences:

>Translated_248_residues
MFSSAFCWCKLPKNEKDPSPVNVPNTPSVTPAEGGQKLPAHELVYQRLRAQILFGELAPGQAVTIQGLVEALDSGMTPVR
EAIRRLISDGALVFQGNRRVSVPELRAEDVDELIYARKTMECELARRAATRIPSASVDALEALDLRLDTAIANGDVAGYL
GLNYEFHSMLNEYADAPILADLADRLWLRFGPSLRVVCGRFGTQSFPDRHKDILEGLRKKDADLVSLAMERDVLQGMEQV
RAGLTPIN
>Mature_248_residues
MFSSAFCWCKLPKNEKDPSPVNVPNTPSVTPAEGGQKLPAHELVYQRLRAQILFGELAPGQAVTIQGLVEALDSGMTPVR
EAIRRLISDGALVFQGNRRVSVPELRAEDVDELIYARKTMECELARRAATRIPSASVDALEALDLRLDTAIANGDVAGYL
GLNYEFHSMLNEYADAPILADLADRLWLRFGPSLRVVCGRFGTQSFPDRHKDILEGLRKKDADLVSLAMERDVLQGMEQV
RAGLTPIN

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=179, Percent_Identity=27.3743016759777, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787821, Length=207, Percent_Identity=27.536231884058, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27330; Mature: 27330

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSSAFCWCKLPKNEKDPSPVNVPNTPSVTPAEGGQKLPAHELVYQRLRAQILFGELAPG
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
QAVTIQGLVEALDSGMTPVREAIRRLISDGALVFQGNRRVSVPELRAEDVDELIYARKTM
CEEHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHH
ECELARRAATRIPSASVDALEALDLRLDTAIANGDVAGYLGLNYEFHSMLNEYADAPILA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCHHH
DLADRLWLRFGPSLRVVCGRFGTQSFPDRHKDILEGLRKKDADLVSLAMERDVLQGMEQV
HHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RAGLTPIN
HHCCCCCC
>Mature Secondary Structure
MFSSAFCWCKLPKNEKDPSPVNVPNTPSVTPAEGGQKLPAHELVYQRLRAQILFGELAPG
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
QAVTIQGLVEALDSGMTPVREAIRRLISDGALVFQGNRRVSVPELRAEDVDELIYARKTM
CEEHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHH
ECELARRAATRIPSASVDALEALDLRLDTAIANGDVAGYLGLNYEFHSMLNEYADAPILA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCHHH
DLADRLWLRFGPSLRVVCGRFGTQSFPDRHKDILEGLRKKDADLVSLAMERDVLQGMEQV
HHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RAGLTPIN
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]