Click here to switch to the map view.

The map label for this gene is cca [H]

Identifier: 99082731

GI number: 99082731

Start: 3059497

End: 3060657

Strand: Direct

Name: cca [H]

Synonym: TM1040_2891

Alternate gene names: 99082731

Gene position: 3059497-3060657 (Clockwise)

Preceding gene: 99082730

Following gene: 99082732

Centisome position: 95.58

GC content: 59.09

Gene sequence:

>1161_bases
ATGAAGATTGATGAACCCTGGCTCCATTCTCCTGCGACGCGCAAACTCTGCGATGCGCTGACCTCTGCCGGAGCGGAAAT
CTATTTCGTCGGCGGTTGCGTGCGCAATGCCCTCCTTGGGGAGCCTGTTTCGGATCTCGACCTTTCCACCAGCGCGACAC
CGCAATATGTCACCGAGGCTGCGGAGCAAGCGGGTCTTAAGGTCATACCAACTGGGATCGAGCACGGCACTGTCACGGTG
GTGGTGGATGGAGACCCCTTTGAAATTACTACCTTTCGCAGGGATGTCGCGACCGATGGGCGCCGGGCGGTCGTAGCATT
TGCCACGGAGATCTTAGAGGACGCGCGACGGCGCGACTTCACAATGAACGCCCTCTATGCGCGCCCCACTGGCGAAATTG
TCGACCCTCTCTCGGGGTTCGAAGACCTCAGGGCGCGCCGGGTGCGGTTTATCGGGGACGCCACGCGACGGATCCAAGAG
GACTACCTGCGCATCCTGCGATATTTCCGTTTTCACAGCTGGTATGGCGATGCCGAGGCCGGGTTTGACCCCGAGGCATT
GGCTGCGATCGCCGCTACGCAGGAGGGGCTGGATCATCTATCGCGTGAGCGGGTTGGCGCAGAGATCATGAAGCTTCTGT
CTTCGTCCAACCCAGCGCCCTCGGTCGCGACCATGCGTCAGATCGGTGTGCTGGCGCATGTCTTGCCTGGTGCGGATGAC
CGTGCGCTTGCCCCCCTTATTCATCTCGAGGCAGATCTGCCAGCAGAGGCGGAAACGCGCCTTGCAGCACTTGGTGGAGA
AGATCTTCAAAATACCCTTCGACTGAGCAAGGCGAGCGCGGCGCGCGTCACGCTCTTACGGACGCTTGCGACCGGGACCG
CCACTGCGGCAGAGATTGCCTATCGTCAAGGTGTCGACACGGCAAAACGCGCCATGATGTTGCGGTCGGCATTGTTTGAG
ATGCCAGTTTTGCCCGATCTAGAGTCTGATATTGCACTTGGTGCAGTCGCTGAGTTCCCGATTTCAGCCAAAGATCTGAT
GCCTCATGTGCAGGGTGCAGCACTTGGACGAGCGCTTAGAACGCTCGAAGCAGAGTGGATCAGGAGTGGTTTTCAAAGCG
ACAGGGAAACGCTTTTGAAGCGGCTGCCGGATCTCAACTAA

Upstream 100 bases:

>100_bases
GCACAAAACGGTTCTACTTTGCGGTGCCATTTGGCCCCTATTACATCTGGGGGGCAACCGCGCGTATGTTGCGCGGATTT
GCTGCGATCATGGACACTCG

Downstream 100 bases:

>100_bases
GTGCCTTGAACGATGCCATGGATTGAAACGCGTGACAAAGCGCGCGGGTCACGCTACCCATAAAGCGTGGAACACACATG
GCAGGAGGAATGCTGATGTA

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MKIDEPWLHSPATRKLCDALTSAGAEIYFVGGCVRNALLGEPVSDLDLSTSATPQYVTEAAEQAGLKVIPTGIEHGTVTV
VVDGDPFEITTFRRDVATDGRRAVVAFATEILEDARRRDFTMNALYARPTGEIVDPLSGFEDLRARRVRFIGDATRRIQE
DYLRILRYFRFHSWYGDAEAGFDPEALAAIAATQEGLDHLSRERVGAEIMKLLSSSNPAPSVATMRQIGVLAHVLPGADD
RALAPLIHLEADLPAEAETRLAALGGEDLQNTLRLSKASAARVTLLRTLATGTATAAEIAYRQGVDTAKRAMMLRSALFE
MPVLPDLESDIALGAVAEFPISAKDLMPHVQGAALGRALRTLEAEWIRSGFQSDRETLLKRLPDLN

Sequences:

>Translated_386_residues
MKIDEPWLHSPATRKLCDALTSAGAEIYFVGGCVRNALLGEPVSDLDLSTSATPQYVTEAAEQAGLKVIPTGIEHGTVTV
VVDGDPFEITTFRRDVATDGRRAVVAFATEILEDARRRDFTMNALYARPTGEIVDPLSGFEDLRARRVRFIGDATRRIQE
DYLRILRYFRFHSWYGDAEAGFDPEALAAIAATQEGLDHLSRERVGAEIMKLLSSSNPAPSVATMRQIGVLAHVLPGADD
RALAPLIHLEADLPAEAETRLAALGGEDLQNTLRLSKASAARVTLLRTLATGTATAAEIAYRQGVDTAKRAMMLRSALFE
MPVLPDLESDIALGAVAEFPISAKDLMPHVQGAALGRALRTLEAEWIRSGFQSDRETLLKRLPDLN
>Mature_386_residues
MKIDEPWLHSPATRKLCDALTSAGAEIYFVGGCVRNALLGEPVSDLDLSTSATPQYVTEAAEQAGLKVIPTGIEHGTVTV
VVDGDPFEITTFRRDVATDGRRAVVAFATEILEDARRRDFTMNALYARPTGEIVDPLSGFEDLRARRVRFIGDATRRIQE
DYLRILRYFRFHSWYGDAEAGFDPEALAAIAATQEGLDHLSRERVGAEIMKLLSSSNPAPSVATMRQIGVLAHVLPGADD
RALAPLIHLEADLPAEAETRLAALGGEDLQNTLRLSKASAARVTLLRTLATGTATAAEIAYRQGVDTAKRAMMLRSALFE
MPVLPDLESDIALGAVAEFPISAKDLMPHVQGAALGRALRTLEAEWIRSGFQSDRETLLKRLPDLN

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=416, Percent_Identity=32.2115384615385, Blast_Score=181, Evalue=9e-46,
Organism=Escherichia coli, GI1789436, Length=220, Percent_Identity=33.1818181818182, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081691, Length=214, Percent_Identity=31.3084112149533, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=423, Percent_Identity=30.4964539007092, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI281360112, Length=394, Percent_Identity=30.4568527918782, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21357337, Length=394, Percent_Identity=30.4568527918782, Blast_Score=162, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 42002; Mature: 42002

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDEPWLHSPATRKLCDALTSAGAEIYFVGGCVRNALLGEPVSDLDLSTSATPQYVTEA
CCCCCCCCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
AEQAGLKVIPTGIEHGTVTVVVDGDPFEITTFRRDVATDGRRAVVAFATEILEDARRRDF
HHHCCCEEEECCCCCCEEEEEECCCCEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
TMNALYARPTGEIVDPLSGFEDLRARRVRFIGDATRRIQEDYLRILRYFRFHSWYGDAEA
CHHEEEECCCCHHHCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GFDPEALAAIAATQEGLDHLSRERVGAEIMKLLSSSNPAPSVATMRQIGVLAHVLPGADD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
RALAPLIHLEADLPAEAETRLAALGGEDLQNTLRLSKASAARVTLLRTLATGTATAAEIA
CHHHHHHEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
YRQGVDTAKRAMMLRSALFEMPVLPDLESDIALGAVAEFPISAKDLMPHVQGAALGRALR
HHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
TLEAEWIRSGFQSDRETLLKRLPDLN
HHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKIDEPWLHSPATRKLCDALTSAGAEIYFVGGCVRNALLGEPVSDLDLSTSATPQYVTEA
CCCCCCCCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
AEQAGLKVIPTGIEHGTVTVVVDGDPFEITTFRRDVATDGRRAVVAFATEILEDARRRDF
HHHCCCEEEECCCCCCEEEEEECCCCEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
TMNALYARPTGEIVDPLSGFEDLRARRVRFIGDATRRIQEDYLRILRYFRFHSWYGDAEA
CHHEEEECCCCHHHCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GFDPEALAAIAATQEGLDHLSRERVGAEIMKLLSSSNPAPSVATMRQIGVLAHVLPGADD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
RALAPLIHLEADLPAEAETRLAALGGEDLQNTLRLSKASAARVTLLRTLATGTATAAEIA
CHHHHHHEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
YRQGVDTAKRAMMLRSALFEMPVLPDLESDIALGAVAEFPISAKDLMPHVQGAALGRALR
HHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
TLEAEWIRSGFQSDRETLLKRLPDLN
HHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]