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The map label for this gene is rsmG

Identifier: 99082706

GI number: 99082706

Start: 3035889

End: 3036506

Strand: Direct

Name: rsmG

Synonym: TM1040_2866

Alternate gene names: 99082706

Gene position: 3035889-3036506 (Clockwise)

Preceding gene: 99082705

Following gene: 99082707

Centisome position: 94.84

GC content: 49.35

Gene sequence:

>618_bases
ATGAGCGATGGTCGAGGAAACTTGGAACTTGATGTTTCACGTGAAACATTAGAACGACTGATGGTCTTCTCAGAGGTCCT
TAAAAAGTGGAACCCGAAGATCAACCTGGTTTCTAGAAAGAGCATCGACGATCTTTGGACCCGTCACATTCTGGATAGCA
TTCAAGTCTTTGATGCCGCTCCAGAGGGCGGGGCTTGGCTAGATATCGGGAGCGGTGGTGGACTTCCTGGCATTATTGTC
GCGATTTTGGCAGCGGAAAAGTCACCGGATACTCGGATTACGCTAATGGAGAGTGATCTGAGAAAATGTGCTTTCCTGCG
TAATGCAGCACGAGAGTGCGGTGTGTCAGTAACGGTTAAGTCGAAACGTATCGAGAGTGCCCCAGAAGAAAATTGTGACG
TCTTATCTGCACGCGCGTTGGCCGATCTTGATAATCTCTTGGGGTTTGCTGAACGTCATCTCGCCGAAAATGGTACAGCG
ATGTTTCCGAAGGGCGCGAACTGGAAAAAAGAGGTGGATAACGCGCGCAAGAGATGGCGGTTTGAGTGCGATGAGATTAC
AAGTGTAACTGAGCCTGGTGCTGTCATCCTCAAGATAAAGGGAGTAGAGCGTGTCTGA

Upstream 100 bases:

>100_bases
GCTTAGCGCAAGCCGCTCGAATTGATGGTATGACCCCAGCCGCACTGGCGCTGATGCTTGGAAGTTTGAAGAAGCTTTCC
AGGGGCGAAAGCAAAGCATC

Downstream 100 bases:

>100_bases
CTTTTCCCGTTTTGGAGTCCCCCGGATCATTGCGATCGCTAACCAAAAGGGGGGCGTGGGAAAAACGACTACTGCGATTA
ACCTCGCCGCCGCTTTGGTA

Product: methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MSDGRGNLELDVSRETLERLMVFSEVLKKWNPKINLVSRKSIDDLWTRHILDSIQVFDAAPEGGAWLDIGSGGGLPGIIV
AILAAEKSPDTRITLMESDLRKCAFLRNAARECGVSVTVKSKRIESAPEENCDVLSARALADLDNLLGFAERHLAENGTA
MFPKGANWKKEVDNARKRWRFECDEITSVTEPGAVILKIKGVERV

Sequences:

>Translated_205_residues
MSDGRGNLELDVSRETLERLMVFSEVLKKWNPKINLVSRKSIDDLWTRHILDSIQVFDAAPEGGAWLDIGSGGGLPGIIV
AILAAEKSPDTRITLMESDLRKCAFLRNAARECGVSVTVKSKRIESAPEENCDVLSARALADLDNLLGFAERHLAENGTA
MFPKGANWKKEVDNARKRWRFECDEITSVTEPGAVILKIKGVERV
>Mature_204_residues
SDGRGNLELDVSRETLERLMVFSEVLKKWNPKINLVSRKSIDDLWTRHILDSIQVFDAAPEGGAWLDIGSGGGLPGIIVA
ILAAEKSPDTRITLMESDLRKCAFLRNAARECGVSVTVKSKRIESAPEENCDVLSARALADLDNLLGFAERHLAENGTAM
FPKGANWKKEVDNARKRWRFECDEITSVTEPGAVILKIKGVERV

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=169, Percent_Identity=30.1775147928994, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_SILST (Q1GCL8)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614860.1
- ProteinModelPortal:   Q1GCL8
- SMR:   Q1GCL8
- STRING:   Q1GCL8
- GeneID:   4076400
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2866
- NMPDR:   fig|292414.1.peg.1769
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   KPLLEIC
- PhylomeDB:   Q1GCL8
- ProtClustDB:   CLSK759030
- BioCyc:   SSP292414:TM1040_2866-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 22676; Mature: 22545

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGRGNLELDVSRETLERLMVFSEVLKKWNPKINLVSRKSIDDLWTRHILDSIQVFDAA
CCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCC
PEGGAWLDIGSGGGLPGIIVAILAAEKSPDTRITLMESDLRKCAFLRNAARECGVSVTVK
CCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEEE
SKRIESAPEENCDVLSARALADLDNLLGFAERHLAENGTAMFPKGANWKKEVDNARKRWR
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHCC
FECDEITSVTEPGAVILKIKGVERV
CCHHHHCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
SDGRGNLELDVSRETLERLMVFSEVLKKWNPKINLVSRKSIDDLWTRHILDSIQVFDAA
CCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCC
PEGGAWLDIGSGGGLPGIIVAILAAEKSPDTRITLMESDLRKCAFLRNAARECGVSVTVK
CCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEEE
SKRIESAPEENCDVLSARALADLDNLLGFAERHLAENGTAMFPKGANWKKEVDNARKRWR
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHCC
FECDEITSVTEPGAVILKIKGVERV
CCHHHHCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA