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The map label for this gene is yhfT [H]

Identifier: 99082629

GI number: 99082629

Start: 2948817

End: 2949971

Strand: Direct

Name: yhfT [H]

Synonym: TM1040_2789

Alternate gene names: 99082629

Gene position: 2948817-2949971 (Clockwise)

Preceding gene: 99082628

Following gene: 99082630

Centisome position: 92.12

GC content: 60.87

Gene sequence:

>1155_bases
ATGACAGCGCGCAAGGACAGATTTCACCTCTCACCAGAGACCCGCATCCTCACGCCCGAGGTGGCAGGTCTGGCGGAATT
GCTGCATGCGCTCCGCGAGGGCCAGTCCATTCTTGCACAACCGGGTGCCGTCCCGATCCAAGCCCAAGCGGAACAGACGC
AGTGGCTCTACTGCAAAAGCTCAGGCTCGACGGGATACGCAAAAACCATTCGGCGCAGCCCCGAAAGTTGGATTCGAAGC
TTCGAGATGGACCGAGAGCTTTTTGCGCTTGGCGCCAGGGATACCTATGCAAGCCTTGGCAACCTTTCGCATTCGCTCAC
GCTATTTGCGGCGGTTGCGGCGTGCCAAATCGGTGCCGACCTCGCGATGCTGGGCGACTGCGGTCCGCGAGAACAGCTTA
GTCGACTGGGCGAGTTCCACGCCTCGGTGCTCTATGCGACGCCAGCGCAGTTGCGACTGCTGCGCCGCGCTGCGGGAAAA
CGTCAACTGGAAGGGCTGCGATTGCTGTTTTCATCGGGTGGCAAGCTCGACCACGAGACGCGCGCCGGGCTTCAGCAGCT
CTTTCCGGGCGCCCAGGTGATTGAGATCTTCGGCGCCACCGAAACAAGTTATATGACCATCGCAGGCCCGGACGCGCTCG
AAGGGTCTGTGGGACAAGCCTACCCTGGCGTTGAAATCAGGATCGACAGCCCTGATGCGAATGGCGTGGGCGAAATCTGG
ATACGGAGTCCGTTTGTCTTTGATGGCTATGTAAAAGGAGAGAGCCAAGAGACACGTTGGAACAACGGGTGGCTCACCAT
CGGAGAGATGGGACGGCTGGACGAGTCGGGCAACCTCTTTGCCAAGGGGCGCAAAAATCGCATGGTGACGGTGGCCGACA
AGAACGTTTTTCCCGAGGAAATCGAAGCCGTCCTGATGAGCGTCCCCGAGGTCGAGATCGCCGTTGTCTGTGCAGTTCCG
GATGCGCTGCGCGGGCATGTGATCCATGCCGCGGTTCAAGGCGAGGCCAGTCCCGCTGCGCTCCGGCAGCTTTGCCGCCA
TCAAATCGGCGATCACGCCGTGCCTCGCAGCATACGCAGGGTGGCGAAGATCCCGATGCTGACGGCGGGCAAACCGGACC
TGCAGGCCTTGCGCGATCTTTGGGACGATCTGTGA

Upstream 100 bases:

>100_bases
CTTCGACCGTACACGCCTGTTTCGCAAATTCACCTGAGACCTGTTCGCCCGCCGCATTCATCGGTAGTTTGCCTCCCAAC
CGGGATGCACAGGCACAGCG

Downstream 100 bases:

>100_bases
TGCCCTCCATTGTCAGCGCGCGCCGTACCGCGGTGATCCCGCGCGGAGGCGCCTTTGCGGCGCTAGAGCCACATGAGCTT
GCGGCCCCTGTGATCCGCGC

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MTARKDRFHLSPETRILTPEVAGLAELLHALREGQSILAQPGAVPIQAQAEQTQWLYCKSSGSTGYAKTIRRSPESWIRS
FEMDRELFALGARDTYASLGNLSHSLTLFAAVAACQIGADLAMLGDCGPREQLSRLGEFHASVLYATPAQLRLLRRAAGK
RQLEGLRLLFSSGGKLDHETRAGLQQLFPGAQVIEIFGATETSYMTIAGPDALEGSVGQAYPGVEIRIDSPDANGVGEIW
IRSPFVFDGYVKGESQETRWNNGWLTIGEMGRLDESGNLFAKGRKNRMVTVADKNVFPEEIEAVLMSVPEVEIAVVCAVP
DALRGHVIHAAVQGEASPAALRQLCRHQIGDHAVPRSIRRVAKIPMLTAGKPDLQALRDLWDDL

Sequences:

>Translated_384_residues
MTARKDRFHLSPETRILTPEVAGLAELLHALREGQSILAQPGAVPIQAQAEQTQWLYCKSSGSTGYAKTIRRSPESWIRS
FEMDRELFALGARDTYASLGNLSHSLTLFAAVAACQIGADLAMLGDCGPREQLSRLGEFHASVLYATPAQLRLLRRAAGK
RQLEGLRLLFSSGGKLDHETRAGLQQLFPGAQVIEIFGATETSYMTIAGPDALEGSVGQAYPGVEIRIDSPDANGVGEIW
IRSPFVFDGYVKGESQETRWNNGWLTIGEMGRLDESGNLFAKGRKNRMVTVADKNVFPEEIEAVLMSVPEVEIAVVCAVP
DALRGHVIHAAVQGEASPAALRQLCRHQIGDHAVPRSIRRVAKIPMLTAGKPDLQALRDLWDDL
>Mature_383_residues
TARKDRFHLSPETRILTPEVAGLAELLHALREGQSILAQPGAVPIQAQAEQTQWLYCKSSGSTGYAKTIRRSPESWIRSF
EMDRELFALGARDTYASLGNLSHSLTLFAAVAACQIGADLAMLGDCGPREQLSRLGEFHASVLYATPAQLRLLRRAAGKR
QLEGLRLLFSSGGKLDHETRAGLQQLFPGAQVIEIFGATETSYMTIAGPDALEGSVGQAYPGVEIRIDSPDANGVGEIWI
RSPFVFDGYVKGESQETRWNNGWLTIGEMGRLDESGNLFAKGRKNRMVTVADKNVFPEEIEAVLMSVPEVEIAVVCAVPD
ALRGHVIHAAVQGEASPAALRQLCRHQIGDHAVPRSIRRVAKIPMLTAGKPDLQALRDLWDDL

Specific function: May be involved in fatty acid metabolism [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=349, Percent_Identity=25.5014326647564, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI156151445, Length=201, Percent_Identity=28.8557213930348, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1789201, Length=364, Percent_Identity=25.5494505494505, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788107, Length=232, Percent_Identity=28.8793103448276, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI145693145, Length=333, Percent_Identity=25.2252252252252, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786810, Length=348, Percent_Identity=26.4367816091954, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17531443, Length=352, Percent_Identity=23.8636363636364, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17538037, Length=228, Percent_Identity=25.8771929824561, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=228, Percent_Identity=24.5614035087719, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=261, Percent_Identity=26.4367816091954, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI32563687, Length=258, Percent_Identity=26.3565891472868, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71985884, Length=383, Percent_Identity=22.976501305483, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17559526, Length=274, Percent_Identity=25.5474452554745, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=376, Percent_Identity=21.8085106382979, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI20130357, Length=224, Percent_Identity=30.8035714285714, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI19921316, Length=149, Percent_Identity=34.8993288590604, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922652, Length=348, Percent_Identity=23.2758620689655, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21355181, Length=370, Percent_Identity=22.1621621621622, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356947, Length=242, Percent_Identity=26.4462809917355, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24581924, Length=287, Percent_Identity=25.4355400696864, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI18859661, Length=330, Percent_Identity=24.5454545454545, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24656500, Length=352, Percent_Identity=23.0113636363636, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648260, Length=296, Percent_Identity=25, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17933690, Length=119, Percent_Identity=33.6134453781513, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24653035, Length=183, Percent_Identity=26.2295081967213, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 41875; Mature: 41744

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARKDRFHLSPETRILTPEVAGLAELLHALREGQSILAQPGAVPIQAQAEQTQWLYCKS
CCCCCCCEECCCCCEEECHHHHHHHHHHHHHHCCCHHHCCCCCCCEEECCCCCEEEEEEC
SGSTGYAKTIRRSPESWIRSFEMDRELFALGARDTYASLGNLSHSLTLFAAVAACQIGAD
CCCCCHHHHHHCCHHHHHHHHHCCCHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCC
LAMLGDCGPREQLSRLGEFHASVLYATPAQLRLLRRAAGKRQLEGLRLLFSSGGKLDHET
EEEECCCCCHHHHHHHHHHHHHHEEECHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHH
RAGLQQLFPGAQVIEIFGATETSYMTIAGPDALEGSVGQAYPGVEIRIDSPDANGVGEIW
HHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCEEE
IRSPFVFDGYVKGESQETRWNNGWLTIGEMGRLDESGNLFAKGRKNRMVTVADKNVFPEE
EECCEEEECEECCCCCCCCCCCCCEEECCCCCCCCCCCEEECCCCCCEEEEECCCCCHHH
IEAVLMSVPEVEIAVVCAVPDALRGHVIHAAVQGEASPAALRQLCRHQIGDHAVPRSIRR
HHHHHHCCCCEEEEEEEECCHHHHCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHH
VAKIPMLTAGKPDLQALRDLWDDL
HHHCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TARKDRFHLSPETRILTPEVAGLAELLHALREGQSILAQPGAVPIQAQAEQTQWLYCKS
CCCCCCEECCCCCEEECHHHHHHHHHHHHHHCCCHHHCCCCCCCEEECCCCCEEEEEEC
SGSTGYAKTIRRSPESWIRSFEMDRELFALGARDTYASLGNLSHSLTLFAAVAACQIGAD
CCCCCHHHHHHCCHHHHHHHHHCCCHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCC
LAMLGDCGPREQLSRLGEFHASVLYATPAQLRLLRRAAGKRQLEGLRLLFSSGGKLDHET
EEEECCCCCHHHHHHHHHHHHHHEEECHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHH
RAGLQQLFPGAQVIEIFGATETSYMTIAGPDALEGSVGQAYPGVEIRIDSPDANGVGEIW
HHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCEEE
IRSPFVFDGYVKGESQETRWNNGWLTIGEMGRLDESGNLFAKGRKNRMVTVADKNVFPEE
EECCEEEECEECCCCCCCCCCCCCEEECCCCCCCCCCCEEECCCCCCEEEEECCCCCHHH
IEAVLMSVPEVEIAVVCAVPDALRGHVIHAAVQGEASPAALRQLCRHQIGDHAVPRSIRR
HHHHHHCCCCEEEEEEEECCHHHHCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHH
VAKIPMLTAGKPDLQALRDLWDDL
HHHCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]