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The map label for this gene is vraB [H]

Identifier: 99082630

GI number: 99082630

Start: 2949971

End: 2951074

Strand: Direct

Name: vraB [H]

Synonym: TM1040_2790

Alternate gene names: 99082630

Gene position: 2949971-2951074 (Clockwise)

Preceding gene: 99082629

Following gene: 99082632

Centisome position: 92.16

GC content: 64.22

Gene sequence:

>1104_bases
ATGCCCTCCATTGTCAGCGCGCGCCGTACCGCGGTGATCCCGCGCGGAGGCGCCTTTGCGGCGCTAGAGCCACATGAGCT
TGCGGCCCCTGTGATCCGCGCCTGCCTGGAAGACGCGGGCCTCGCGGGGGATGAGGTCGGAGAGCTGATCCTGTCCAATG
CCTTGGGTGCAGGGGGAAATCCAGCGCGGGTAGCAGCGCTGGCGGCTGGGCTGCCAGACCATGTCGCGGGGCTCAGTATA
GACCGCCAATGCGCGGGCGGACTGGATGCAATCCTACTTGGGCATGCATTGGTTCAGGCTGGTCACCATGAGGTCGTGAT
CGCCGGTGGCGCAGAAAGCTATTCCCGCCGTCCGCAGCGCAGCCGCACTTTCGCCGATGGACGCCCACCAGAGCCTTACG
CGCAAGCACGATTTACCCCGTGGCCGGATCGTGATCCGGATATGGCGGTGGCCGCGGCAGATCTGGCACGCATGCAGCAC
ATCACCCGAGCAGAGCAAGATGACTGGGCCCAGCGCAGCCATGCTCTCGCAAGCTTGCATGAAGCCAGACGCGCCGAAGA
GATCGTTTCAATTAACGGCGCGGTCAAAGATAGTTTCACGCGCACCCTCACCGCCCGCCACTGCGCGCGCGCCAAAGTTG
TCACTGGCGACATAACGGCTGCGAATATGGCGGTGGCGGCGGATGCGGCGGCCTTTGTGGTTATTGTGAGCGAAAAAATC
GCGCGGCGTCTGCGCACGCGAGGGCTTTCGCTGATTGCAGGCGCAACACTCGGCGCGGACCCAGAGCAGCCAGGCCTCGC
CCCGCTGCCCGCAATCCAAAAAGTGCTCACTCAGGCGCAAATTGAGCGCACAGATCTATGCCGCGCAGAAATCATGGAAG
CCTTCGCAGTGCAGGCACTGGCCTGCGTGCGCGGCGCAGCTTTGCCCGCGGACATCGTTAACCCCGCAGGAGGCGCACTG
GCACAAGGTCATCCCGTGGGCGCTTCGGGCGCTCTCCTCGTGGTACGACTGTTTCACGCGCTGCGCTGCGGAGATGGAAT
TGCTCTGGCCGCCATTGCCGCGGCTGGTGGCATAGGCTCTGCTGCCCTCTTTGAGGCTACATGA

Upstream 100 bases:

>100_bases
ACGCCGTGCCTCGCAGCATACGCAGGGTGGCGAAGATCCCGATGCTGACGGCGGGCAAACCGGACCTGCAGGCCTTGCGC
GATCTTTGGGACGATCTGTG

Downstream 100 bases:

>100_bases
AAATGTGAAAAAGGCGCCGCGAAATGCAGCGCCTTTCCAATCATCGTAGATCCACCCAGATCACTTCAGATCAAAGCGAT
CCAGTTCCATCACCTTGACC

Product: Acetyl-CoA C-acetyltransferase

Products: Acetoacetyl-CoA; Coenzyme A [C]

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MPSIVSARRTAVIPRGGAFAALEPHELAAPVIRACLEDAGLAGDEVGELILSNALGAGGNPARVAALAAGLPDHVAGLSI
DRQCAGGLDAILLGHALVQAGHHEVVIAGGAESYSRRPQRSRTFADGRPPEPYAQARFTPWPDRDPDMAVAAADLARMQH
ITRAEQDDWAQRSHALASLHEARRAEEIVSINGAVKDSFTRTLTARHCARAKVVTGDITAANMAVAADAAAFVVIVSEKI
ARRLRTRGLSLIAGATLGADPEQPGLAPLPAIQKVLTQAQIERTDLCRAEIMEAFAVQALACVRGAALPADIVNPAGGAL
AQGHPVGASGALLVVRLFHALRCGDGIALAAIAAAGGIGSAALFEAT

Sequences:

>Translated_367_residues
MPSIVSARRTAVIPRGGAFAALEPHELAAPVIRACLEDAGLAGDEVGELILSNALGAGGNPARVAALAAGLPDHVAGLSI
DRQCAGGLDAILLGHALVQAGHHEVVIAGGAESYSRRPQRSRTFADGRPPEPYAQARFTPWPDRDPDMAVAAADLARMQH
ITRAEQDDWAQRSHALASLHEARRAEEIVSINGAVKDSFTRTLTARHCARAKVVTGDITAANMAVAADAAAFVVIVSEKI
ARRLRTRGLSLIAGATLGADPEQPGLAPLPAIQKVLTQAQIERTDLCRAEIMEAFAVQALACVRGAALPADIVNPAGGAL
AQGHPVGASGALLVVRLFHALRCGDGIALAAIAAAGGIGSAALFEAT
>Mature_366_residues
PSIVSARRTAVIPRGGAFAALEPHELAAPVIRACLEDAGLAGDEVGELILSNALGAGGNPARVAALAAGLPDHVAGLSID
RQCAGGLDAILLGHALVQAGHHEVVIAGGAESYSRRPQRSRTFADGRPPEPYAQARFTPWPDRDPDMAVAAADLARMQHI
TRAEQDDWAQRSHALASLHEARRAEEIVSINGAVKDSFTRTLTARHCARAKVVTGDITAANMAVAADAAAFVVIVSEKIA
RRLRTRGLSLIAGATLGADPEQPGLAPLPAIQKVLTQAQIERTDLCRAEIMEAFAVQALACVRGAALPADIVNPAGGALA
QGHPVGASGALLVVRLFHALRCGDGIALAAIAAAGGIGSAALFEAT

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4557237, Length=388, Percent_Identity=30.6701030927835, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI148539872, Length=391, Percent_Identity=30.690537084399, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI4501853, Length=394, Percent_Identity=31.2182741116751, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=28.7917737789203, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4504327, Length=416, Percent_Identity=26.6826923076923, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI194353979, Length=369, Percent_Identity=26.5582655826558, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI87082165, Length=390, Percent_Identity=34.8717948717949, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI1787663, Length=402, Percent_Identity=34.3283582089552, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1788554, Length=387, Percent_Identity=32.5581395348837, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI48994986, Length=398, Percent_Identity=29.3969849246231, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788683, Length=402, Percent_Identity=24.8756218905473, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25147385, Length=384, Percent_Identity=29.9479166666667, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI133906874, Length=392, Percent_Identity=30.8673469387755, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17535921, Length=389, Percent_Identity=28.0205655526992, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=28.3720930232558, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17535917, Length=382, Percent_Identity=25.9162303664921, Blast_Score=95, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6325229, Length=395, Percent_Identity=32.9113924050633, Blast_Score=135, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6322031, Length=325, Percent_Identity=29.5384615384615, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24655093, Length=390, Percent_Identity=34.1025641025641, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24640423, Length=392, Percent_Identity=31.1224489795918, Blast_Score=151, Evalue=5e-37,
Organism=Drosophila melanogaster, GI17648125, Length=393, Percent_Identity=30.5343511450382, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17137578, Length=425, Percent_Identity=27.7647058823529, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.9 [C]

Molecular weight: Translated: 37728; Mature: 37597

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSIVSARRTAVIPRGGAFAALEPHELAAPVIRACLEDAGLAGDEVGELILSNALGAGGN
CCCCCCCHHHEECCCCCCEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
PARVAALAAGLPDHVAGLSIDRQCAGGLDAILLGHALVQAGHHEVVIAGGAESYSRRPQR
HHHHHHHHCCCCHHHHCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHH
SRTFADGRPPEPYAQARFTPWPDRDPDMAVAAADLARMQHITRAEQDDWAQRSHALASLH
HCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
EARRAEEIVSINGAVKDSFTRTLTARHCARAKVVTGDITAANMAVAADAAAFVVIVSEKI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
ARRLRTRGLSLIAGATLGADPEQPGLAPLPAIQKVLTQAQIERTDLCRAEIMEAFAVQAL
HHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ACVRGAALPADIVNPAGGALAQGHPVGASGALLVVRLFHALRCGDGIALAAIAAAGGIGS
HHHHCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
AALFEAT
CHHCCCC
>Mature Secondary Structure 
PSIVSARRTAVIPRGGAFAALEPHELAAPVIRACLEDAGLAGDEVGELILSNALGAGGN
CCCCCCHHHEECCCCCCEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
PARVAALAAGLPDHVAGLSIDRQCAGGLDAILLGHALVQAGHHEVVIAGGAESYSRRPQR
HHHHHHHHCCCCHHHHCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHH
SRTFADGRPPEPYAQARFTPWPDRDPDMAVAAADLARMQHITRAEQDDWAQRSHALASLH
HCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
EARRAEEIVSINGAVKDSFTRTLTARHCARAKVVTGDITAANMAVAADAAAFVVIVSEKI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
ARRLRTRGLSLIAGATLGADPEQPGLAPLPAIQKVLTQAQIERTDLCRAEIMEAFAVQAL
HHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ACVRGAALPADIVNPAGGALAQGHPVGASGALLVVRLFHALRCGDGIALAAIAAAGGIGS
HHHHCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
AALFEAT
CHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acetyl-CoA [C]

Specific reaction: (2) Acetyl-CoA <==> Acetoacetyl-CoA + Coenzyme A [C]

General reaction: Acyl group transfer; Condensation; Thiolytic cleavage [C]

Inhibitor: Acetoacetyl-CoA; Borohydride; Iodoacetamide; N-Ethyl maleimide; p-Chloromercuribenzoate; Sulfhydryl reagents [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]