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The map label for this gene is yiaO [H]

Identifier: 99082590

GI number: 99082590

Start: 2898076

End: 2899119

Strand: Direct

Name: yiaO [H]

Synonym: TM1040_2750

Alternate gene names: 99082590

Gene position: 2898076-2899119 (Clockwise)

Preceding gene: 99082589

Following gene: 99082591

Centisome position: 90.54

GC content: 60.73

Gene sequence:

>1044_bases
ATGAAATCAGTCCTGAAGACAGCTGCCGTTGGCGCGATCGCCGCCCTGACCGCAAGCTCCGCCATGGCCGGTGGCACCAT
CCGCTTCGGCCACGACAACAAGACCGATCCGTTTGAAAACCCGGCTCACGCCTGTACCGCCGTGTTCCAGAACATCGTCA
CCGCAGACACCAACGGCAGCGTCACGGTCGAAGTCTTCGGTTCCAACCAGCTTGGCACTGCAGCAGAGCACGTCCAGCAG
GTGCGCGATGGCGTCATTCAGGCAACACTCACCTCCACCGGTGCGCTTGCGTCTTATTATCCTCGCATCGACGTGCTGAA
CCTGCCGTTTGCATTTGCCAACAACGCCTCGACCTATTCCGTGTTCGACGGTGAATTCGGCGAGGCCCTGGCCGCGGACA
TCGAAGCCGAGCTCGGCGATGTCGTTGTGCTTGGTTTCCCGGACACCGGCGGCTTTTTTGCGGTGACCAACTCTCAGCGC
CAGATCGCGAATCTTGAGGATTTCGACGGCATCCGCATCCGCACCATGACCCTGCCGAGCCACCAGAAGATCGTGCAGGC
GCTTGGGGCCGAGGCCTATCCGCTCAGCTGGGGTGAGGTCTATTCTGGCCTGCAAACCGGCGTAATTGACGGTCAGATGA
ACCCGGTGCCGATCATCTCCTTTGCGAACTTCGCAGAGGTGCAAAAGCACATGACGCTCACCAACCACCTGTTCTCGCCC
TACACCTTCATGGTCAACAAGGCGTTCTATGACGGTCTGACCGAAGCGGAGCAGAACACCCTGCACACCGCCGCCGAAAG
CTGCGTCACCGCGAGCCGCGGCCTGTCGCGCATCATCGAGGCATCCGATCGTGGTCTGGCGGGTCTGATGGACAAGATCG
AAGTGACCGCCCTCTCCAGCGAGCAGCGTGCCGCGATGGCCGGTGCCACACAGCCCGCGTTTGAGACCCATGTGGCGGAG
AACCTCGACGCCAAGGCTGGTGAACTCCTGGCGCTCTTCAAATCCGAAGTCGGGAAGGCAAACGACGCCTCTTATCTCGA
CTAA

Upstream 100 bases:

>100_bases
TGGGAGGACGCGGCTCTGTTGCTGCCGCGTGCGCTCGGATTTGCCCCCTAACCGGGGCCAAACAACCCCACCTTCAAAAA
CCAACAGTGAGGAAAAACAA

Downstream 100 bases:

>100_bases
CGGTCCCCGACCGGGGCGTGGGTTCGCTCACGCCCCGGAACCTCAATTTCAAGGATTTGTCTCATGCGCGTCTTTTGCGC
TTCTCTTGCCACAGAAACCA

Product: TRAP dicarboxylate transporter, DctP subunit

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKSVLKTAAVGAIAALTASSAMAGGTIRFGHDNKTDPFENPAHACTAVFQNIVTADTNGSVTVEVFGSNQLGTAAEHVQQ
VRDGVIQATLTSTGALASYYPRIDVLNLPFAFANNASTYSVFDGEFGEALAADIEAELGDVVVLGFPDTGGFFAVTNSQR
QIANLEDFDGIRIRTMTLPSHQKIVQALGAEAYPLSWGEVYSGLQTGVIDGQMNPVPIISFANFAEVQKHMTLTNHLFSP
YTFMVNKAFYDGLTEAEQNTLHTAAESCVTASRGLSRIIEASDRGLAGLMDKIEVTALSSEQRAAMAGATQPAFETHVAE
NLDAKAGELLALFKSEVGKANDASYLD

Sequences:

>Translated_347_residues
MKSVLKTAAVGAIAALTASSAMAGGTIRFGHDNKTDPFENPAHACTAVFQNIVTADTNGSVTVEVFGSNQLGTAAEHVQQ
VRDGVIQATLTSTGALASYYPRIDVLNLPFAFANNASTYSVFDGEFGEALAADIEAELGDVVVLGFPDTGGFFAVTNSQR
QIANLEDFDGIRIRTMTLPSHQKIVQALGAEAYPLSWGEVYSGLQTGVIDGQMNPVPIISFANFAEVQKHMTLTNHLFSP
YTFMVNKAFYDGLTEAEQNTLHTAAESCVTASRGLSRIIEASDRGLAGLMDKIEVTALSSEQRAAMAGATQPAFETHVAE
NLDAKAGELLALFKSEVGKANDASYLD
>Mature_347_residues
MKSVLKTAAVGAIAALTASSAMAGGTIRFGHDNKTDPFENPAHACTAVFQNIVTADTNGSVTVEVFGSNQLGTAAEHVQQ
VRDGVIQATLTSTGALASYYPRIDVLNLPFAFANNASTYSVFDGEFGEALAADIEAELGDVVVLGFPDTGGFFAVTNSQR
QIANLEDFDGIRIRTMTLPSHQKIVQALGAEAYPLSWGEVYSGLQTGVIDGQMNPVPIISFANFAEVQKHMTLTNHLFSP
YTFMVNKAFYDGLTEAEQNTLHTAAESCVTASRGLSRIIEASDRGLAGLMDKIEVTALSSEQRAAMAGATQPAFETHVAE
NLDAKAGELLALFKSEVGKANDASYLD

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=276, Percent_Identity=30.7971014492754, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36757; Mature: 36757

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVLKTAAVGAIAALTASSAMAGGTIRFGHDNKTDPFENPAHACTAVFQNIVTADTNGS
CCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
VTVEVFGSNQLGTAAEHVQQVRDGVIQATLTSTGALASYYPRIDVLNLPFAFANNASTYS
EEEEEECCCCCCHHHHHHHHHHHHHEEEEHHCCCCHHHHCCCCEEEECCEEECCCCCEEE
VFDGEFGEALAADIEAELGDVVVLGFPDTGGFFAVTNSQRQIANLEDFDGIRIRTMTLPS
EECCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCHHHCCCCCCCCEEEEEEECCC
HQKIVQALGAEAYPLSWGEVYSGLQTGVIDGQMNPVPIISFANFAEVQKHMTLTNHLFSP
HHHHHHHHCCCCCCCCHHHHHCCHHHCEECCCCCCEEEEEHHHHHHHHHHHHHHHHHCCC
YTFMVNKAFYDGLTEAEQNTLHTAAESCVTASRGLSRIIEASDRGLAGLMDKIEVTALSS
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEECC
EQRAAMAGATQPAFETHVAENLDAKAGELLALFKSEVGKANDASYLD
HHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKSVLKTAAVGAIAALTASSAMAGGTIRFGHDNKTDPFENPAHACTAVFQNIVTADTNGS
CCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
VTVEVFGSNQLGTAAEHVQQVRDGVIQATLTSTGALASYYPRIDVLNLPFAFANNASTYS
EEEEEECCCCCCHHHHHHHHHHHHHEEEEHHCCCCHHHHCCCCEEEECCEEECCCCCEEE
VFDGEFGEALAADIEAELGDVVVLGFPDTGGFFAVTNSQRQIANLEDFDGIRIRTMTLPS
EECCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCHHHCCCCCCCCEEEEEEECCC
HQKIVQALGAEAYPLSWGEVYSGLQTGVIDGQMNPVPIISFANFAEVQKHMTLTNHLFSP
HHHHHHHHCCCCCCCCHHHHHCCHHHCEECCCCCCEEEEEHHHHHHHHHHHHHHHHHCCC
YTFMVNKAFYDGLTEAEQNTLHTAAESCVTASRGLSRIIEASDRGLAGLMDKIEVTALSS
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEECC
EQRAAMAGATQPAFETHVAENLDAKAGELLALFKSEVGKANDASYLD
HHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]