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The map label for this gene is iclR [H]

Identifier: 99082538

GI number: 99082538

Start: 2839728

End: 2840570

Strand: Reverse

Name: iclR [H]

Synonym: TM1040_2698

Alternate gene names: 99082538

Gene position: 2840570-2839728 (Counterclockwise)

Preceding gene: 99082543

Following gene: 99082537

Centisome position: 88.74

GC content: 62.99

Gene sequence:

>843_bases
ATGGATTCGCAAGAAAATGCCTCAAGAGCCACCAGACGGGCACGGGGCCGTCCGCGCGACTGGCACGATAAAACCGCGCA
GAACACCATCAAATCGCTCGATCGCGCGATGGAGGTCTTTGAGTATCTGAGCCAGGCGCAGGGCAAGCCGCTTACTATGA
TTGCCGAGGAAATGGGGCAGTCACCCGCCACCGTTTACCGCATTCTTGTCACGCTCGAAGGGCGCGGATTGGTGGACTTC
GACGCCGAAGAGCAGCTCTGGCACATCGGCGCGCAGGCCTTTGTGATCGGGGCACGGTTCTTGCGCCGCACCAGTCTCGT
GGATCGGGCCCGGCCTATCCTGCGCCGCCTGATGGAGGACACCGGCGAGACTGCGAACCTCGGGGTCGAGAAAGAGGGCC
ATGTTCTGTTCCTGAGCCAGGTGGAGACCCACGCCAGCATTCGCGCGTTCTTTCCGCCGGGCACCCTGTCACAGATGCAC
GCCTCCGGGATTGGCAAGGCGCTCCTGGCGGAAATGGACGGCAGCCGTCTCGACCGGCTCCTTGCGGCGACGCGATTGGA
GATATTTACCGACCACACCATCACCGATCCCGAGGCGCTCAAGGCCGATCTGGCGGCCATCCGTGCGCAAGGGTTCGCGG
TCGACAACGAGGAAAAGAACGCCGGCATGCGCTGTATCGCGGCGCCGGTGTTCGACATGAACCGAGAGGCGATCGCGGGG
ATCTCGGTCTCTGGTCCGACCAGTCGCATCAGCGTCGAGAGCATCGCAGAACTCAGTCGCCCTGTTATCGAAGCAGCGCA
GCAATTGACCGTCGCGATTGGTGGCGATCCGGCCGCGCTCTAG

Upstream 100 bases:

>100_bases
CGTTTTTTAAAAATGGAGTGACGCCAAAAAACGTTTCTTGTCAGGTGCTTGCGTTTTTCATATCGTGAATGTGTATTTCG
CAATGTGCCGGGAGGATCTT

Downstream 100 bases:

>100_bases
CGGCGCCTGATTGCGCCAGTCGCGGCACATCGCGAAAAAATCTCGGAACATATGCGCCAGCGCTCTCGTTGAGTGGATGC
AAGCGGCCGCACCACATGAC

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MDSQENASRATRRARGRPRDWHDKTAQNTIKSLDRAMEVFEYLSQAQGKPLTMIAEEMGQSPATVYRILVTLEGRGLVDF
DAEEQLWHIGAQAFVIGARFLRRTSLVDRARPILRRLMEDTGETANLGVEKEGHVLFLSQVETHASIRAFFPPGTLSQMH
ASGIGKALLAEMDGSRLDRLLAATRLEIFTDHTITDPEALKADLAAIRAQGFAVDNEEKNAGMRCIAAPVFDMNREAIAG
ISVSGPTSRISVESIAELSRPVIEAAQQLTVAIGGDPAAL

Sequences:

>Translated_280_residues
MDSQENASRATRRARGRPRDWHDKTAQNTIKSLDRAMEVFEYLSQAQGKPLTMIAEEMGQSPATVYRILVTLEGRGLVDF
DAEEQLWHIGAQAFVIGARFLRRTSLVDRARPILRRLMEDTGETANLGVEKEGHVLFLSQVETHASIRAFFPPGTLSQMH
ASGIGKALLAEMDGSRLDRLLAATRLEIFTDHTITDPEALKADLAAIRAQGFAVDNEEKNAGMRCIAAPVFDMNREAIAG
ISVSGPTSRISVESIAELSRPVIEAAQQLTVAIGGDPAAL
>Mature_280_residues
MDSQENASRATRRARGRPRDWHDKTAQNTIKSLDRAMEVFEYLSQAQGKPLTMIAEEMGQSPATVYRILVTLEGRGLVDF
DAEEQLWHIGAQAFVIGARFLRRTSLVDRARPILRRLMEDTGETANLGVEKEGHVLFLSQVETHASIRAFFPPGTLSQMH
ASGIGKALLAEMDGSRLDRLLAATRLEIFTDHTITDPEALKADLAAIRAQGFAVDNEEKNAGMRCIAAPVFDMNREAIAG
ISVSGPTSRISVESIAELSRPVIEAAQQLTVAIGGDPAAL

Specific function: Regulation of the glyoxylate bypass operon (aceBAK), which encodes isocitrate lyase, malate synthase as well as isocitrate dehydrogenase kinase/phosphorylase. Glyoxylate disrupts the interaction with the promoter by favoring the inactive dimeric form. Pyr

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI87082359, Length=264, Percent_Identity=37.8787878787879, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1786715, Length=263, Percent_Identity=31.5589353612167, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1788131, Length=247, Percent_Identity=30.7692307692308, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1786468, Length=251, Percent_Identity=30.6772908366534, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1788581, Length=247, Percent_Identity=30.7692307692308, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1789999, Length=250, Percent_Identity=29.2, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790752, Length=194, Percent_Identity=27.8350515463918, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 30577; Mature: 30577

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSQENASRATRRARGRPRDWHDKTAQNTIKSLDRAMEVFEYLSQAQGKPLTMIAEEMGQ
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
SPATVYRILVTLEGRGLVDFDAEEQLWHIGAQAFVIGARFLRRTSLVDRARPILRRLMED
CHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGETANLGVEKEGHVLFLSQVETHASIRAFFPPGTLSQMHASGIGKALLAEMDGSRLDRL
CCCCCCCCCCCCCCEEEEEEHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
LAATRLEIFTDHTITDPEALKADLAAIRAQGFAVDNEEKNAGMRCIAAPVFDMNREAIAG
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHCCCHHHCC
ISVSGPTSRISVESIAELSRPVIEAAQQLTVAIGGDPAAL
EECCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCC
>Mature Secondary Structure
MDSQENASRATRRARGRPRDWHDKTAQNTIKSLDRAMEVFEYLSQAQGKPLTMIAEEMGQ
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
SPATVYRILVTLEGRGLVDFDAEEQLWHIGAQAFVIGARFLRRTSLVDRARPILRRLMED
CHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGETANLGVEKEGHVLFLSQVETHASIRAFFPPGTLSQMHASGIGKALLAEMDGSRLDRL
CCCCCCCCCCCCCCEEEEEEHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
LAATRLEIFTDHTITDPEALKADLAAIRAQGFAVDNEEKNAGMRCIAAPVFDMNREAIAG
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHCCCHHHCC
ISVSGPTSRISVESIAELSRPVIEAAQQLTVAIGGDPAAL
EECCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185227; 1995431; 8265357; 9278503; 1995429 [H]