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The map label for this gene is rnhB

Identifier: 99082490

GI number: 99082490

Start: 2785589

End: 2786278

Strand: Reverse

Name: rnhB

Synonym: TM1040_2650

Alternate gene names: 99082490

Gene position: 2786278-2785589 (Counterclockwise)

Preceding gene: 99082491

Following gene: 99082489

Centisome position: 87.05

GC content: 61.59

Gene sequence:

>690_bases
ATGCGCCCCTTTTTTTGTGCCGCTTTTGGTGCGCCCTCTTGCATGCGCGCGGCAAACGCGCTCAGTGGCGGGATGGACTA
TCCGGATTTATCACTCGAGGAGGCGGCGCAGGCGCGCGGCTATACGCGGATTGCAGGCGTGGATGAGGTCGGGCGCGGCC
CGCTGGCGGGACCGGTCACGGCCGCCGCGGTGGTGCTCGATCTTGCGGATCTGCCCGAAGGGCTCAATGATTCCAAGAAG
CTGACGCCGCGCAGACGGGCCGCGCTGGAACCGGAAATCATGGCGCGCGCCTCTTATGCGGTGGCCCATGCCAGCGTCGA
GGAAATCGACCACCACAATATCTTGCGTGCGTCACATCTGGCGATGGAGCGCGCGGTGGCCGCCCTCGACCCGCAGCCGG
ATTACCTGCTGATCGATGGCAATCTGATCCCGCGCGCGCTGCACATTCCGGCCGAAGCGGTGGTCAAAGGGGACGGGCGC
TCTTTGTCAATTGCCGCGGCCTCAATTCTGGCCAAGGAAGCCCGTGATCGGATCATGGTGGATTTAGCGCAACAGTTTCC
CGGATATGGCTGGGAGCGAAATGCGGGCTATCCGTCGAAACAACACCGCGATGCGCTCATAAAAATAGGGGTGACCCCAC
ATCATAGAAGGTCGTTCAAGCCGGTCCACAAGATATTGTATCAAGAGTGA

Upstream 100 bases:

>100_bases
CGCGGCTATCGTCCGGCCTGCCTGCGTCAATACGGCTACCGACTGGTGCGGCTCTAAGCACCAGCGGCACATTCGAGCAG
CAGACTGGGGCGCATCTGGC

Downstream 100 bases:

>100_bases
ACCGTAGCCTGCTGATTCAAAAAACAAATTGACCTCGAATCCGGGATGACTCATCTTGTGGATAACCAGAATGAGCGCAA
AAGCGCCGTGGTTATCGAGG

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MRPFFCAAFGAPSCMRAANALSGGMDYPDLSLEEAAQARGYTRIAGVDEVGRGPLAGPVTAAAVVLDLADLPEGLNDSKK
LTPRRRAALEPEIMARASYAVAHASVEEIDHHNILRASHLAMERAVAALDPQPDYLLIDGNLIPRALHIPAEAVVKGDGR
SLSIAAASILAKEARDRIMVDLAQQFPGYGWERNAGYPSKQHRDALIKIGVTPHHRRSFKPVHKILYQE

Sequences:

>Translated_229_residues
MRPFFCAAFGAPSCMRAANALSGGMDYPDLSLEEAAQARGYTRIAGVDEVGRGPLAGPVTAAAVVLDLADLPEGLNDSKK
LTPRRRAALEPEIMARASYAVAHASVEEIDHHNILRASHLAMERAVAALDPQPDYLLIDGNLIPRALHIPAEAVVKGDGR
SLSIAAASILAKEARDRIMVDLAQQFPGYGWERNAGYPSKQHRDALIKIGVTPHHRRSFKPVHKILYQE
>Mature_229_residues
MRPFFCAAFGAPSCMRAANALSGGMDYPDLSLEEAAQARGYTRIAGVDEVGRGPLAGPVTAAAVVLDLADLPEGLNDSKK
LTPRRRAALEPEIMARASYAVAHASVEEIDHHNILRASHLAMERAVAALDPQPDYLLIDGNLIPRALHIPAEAVVKGDGR
SLSIAAASILAKEARDRIMVDLAQQFPGYGWERNAGYPSKQHRDALIKIGVTPHHRRSFKPVHKILYQE

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=184, Percent_Identity=54.8913043478261, Blast_Score=172, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_SILST (Q1GD84)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614644.1
- ProteinModelPortal:   Q1GD84
- SMR:   Q1GD84
- STRING:   Q1GD84
- GeneID:   4077953
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2650
- NMPDR:   fig|292414.1.peg.122
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   Q1GD84
- ProtClustDB:   PRK00015
- BioCyc:   SSP292414:TM1040_2650-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 24799; Mature: 24799

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPFFCAAFGAPSCMRAANALSGGMDYPDLSLEEAAQARGYTRIAGVDEVGRGPLAGPVT
CCCEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEECCCHHCCCCCCCCHHH
AAAVVLDLADLPEGLNDSKKLTPRRRAALEPEIMARASYAVAHASVEEIDHHNILRASHL
HHHHHHHHHHCCCCCCCCHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMERAVAALDPQPDYLLIDGNLIPRALHIPAEAVVKGDGRSLSIAAASILAKEARDRIMV
HHHHHHHHCCCCCCEEEEECCCCCCEEECCHHHEEECCCCEEHHHHHHHHHHHHHHHHHH
DLAQQFPGYGWERNAGYPSKQHRDALIKIGVTPHHRRSFKPVHKILYQE
HHHHHCCCCCCCCCCCCCCHHCCCEEEEECCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MRPFFCAAFGAPSCMRAANALSGGMDYPDLSLEEAAQARGYTRIAGVDEVGRGPLAGPVT
CCCEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEECCCHHCCCCCCCCHHH
AAAVVLDLADLPEGLNDSKKLTPRRRAALEPEIMARASYAVAHASVEEIDHHNILRASHL
HHHHHHHHHHCCCCCCCCHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMERAVAALDPQPDYLLIDGNLIPRALHIPAEAVVKGDGRSLSIAAASILAKEARDRIMV
HHHHHHHHCCCCCCEEEEECCCCCCEEECCHHHEEECCCCEEHHHHHHHHHHHHHHHHHH
DLAQQFPGYGWERNAGYPSKQHRDALIKIGVTPHHRRSFKPVHKILYQE
HHHHHCCCCCCCCCCCCCCHHCCCEEEEECCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA