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The map label for this gene is sseA [H]

Identifier: 99082474

GI number: 99082474

Start: 2767830

End: 2768681

Strand: Direct

Name: sseA [H]

Synonym: TM1040_2634

Alternate gene names: 99082474

Gene position: 2767830-2768681 (Clockwise)

Preceding gene: 99082473

Following gene: 99082475

Centisome position: 86.47

GC content: 63.85

Gene sequence:

>852_bases
ATGGACGATCCAAAAACCCTGGTTTCCACCGACTGGCTGGCCGCGCATCTGAAAGATCCCGACCTCAGGATCCTTGATGC
TTCTTGGTATCTGCCGCAGATGGAGCGCGACGCAAAGGCCGAGTATGACGCCGAGCACATCCCCGGCGCGCGCTTCTTTG
ACATCGACGAGATTTCCGATCACCGCTCCGACCTGCCGCATATGGCGCCGCCGGTCGAGAAATTCATGTCCCGCCTGCGC
GCCATGGGCGTCGGCGACGGGCACCAGGTGGTGATCTATGATGGCGCGGGGCTGTTTTCCGCCGCCCGAGTCTGGTGGCT
CTTCCGTCTGATGGGACAGGTCAATGTGGCCGTGCTCGACGGTGGCCTCCCGAAGTGGAAAGCCGAAGGCCGCCCGCTCG
AGGATCTGCCGCCCGTGATCCGCGACCGTCACATGACCGTGCGGGTGCAAAACCACCTTGTGCGCGACGTGACCCAGGTG
TCCTCTGCTGCCAAGCTCGGCGATCACGAGATCATCGACGCCCGCGCCGCCGAACGCTTTCGCGGTGAAGCGCCCGAACC
CCGTCCGGGCATGCGCGCAGGTCACATCCCGGGCTCCAAGAACGTCCCCTTCACCACGCTTCTCAATGACGACGGCACGA
TGAAGGACGCAGAGACCCTGCGCAGCCTCTTTGAAGCCGCCGGTGTTGATCTCACAAAGCCTGCCATCACCTCTTGCGGA
TCGGGCGTCACCGCCGCCGTGCTCAGCCTCGCCATGGAGCGCATGGGCAAGCACGATCACGCGCTCTATGACGGCTCATG
GAGCGAATGGGGCGCCTTCCCCACTCTTCCCGTTGCAACCGGAGACAACTGA

Upstream 100 bases:

>100_bases
CAACAGGCGGTCTGCGCGGGAAACCATGGCAAGCGGCCTTGCACGACCGGGGCGCGGGTAGTAGGCATTTTGCCAACGCA
AGTCCCGGGAGGCGTTGAGA

Downstream 100 bases:

>100_bases
GACATGTTCGAGAACCTCAAACCCCAGCCTGCCGACAAGATTCTGGCGCTGATGCAGATGTACCGCGAAGATCCGCGGGA
CCAGAAGGTCGATCTGGGTG

Product: 3-mercaptopyruvate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MDDPKTLVSTDWLAAHLKDPDLRILDASWYLPQMERDAKAEYDAEHIPGARFFDIDEISDHRSDLPHMAPPVEKFMSRLR
AMGVGDGHQVVIYDGAGLFSAARVWWLFRLMGQVNVAVLDGGLPKWKAEGRPLEDLPPVIRDRHMTVRVQNHLVRDVTQV
SSAAKLGDHEIIDARAAERFRGEAPEPRPGMRAGHIPGSKNVPFTTLLNDDGTMKDAETLRSLFEAAGVDLTKPAITSCG
SGVTAAVLSLAMERMGKHDHALYDGSWSEWGAFPTLPVATGDN

Sequences:

>Translated_283_residues
MDDPKTLVSTDWLAAHLKDPDLRILDASWYLPQMERDAKAEYDAEHIPGARFFDIDEISDHRSDLPHMAPPVEKFMSRLR
AMGVGDGHQVVIYDGAGLFSAARVWWLFRLMGQVNVAVLDGGLPKWKAEGRPLEDLPPVIRDRHMTVRVQNHLVRDVTQV
SSAAKLGDHEIIDARAAERFRGEAPEPRPGMRAGHIPGSKNVPFTTLLNDDGTMKDAETLRSLFEAAGVDLTKPAITSCG
SGVTAAVLSLAMERMGKHDHALYDGSWSEWGAFPTLPVATGDN
>Mature_283_residues
MDDPKTLVSTDWLAAHLKDPDLRILDASWYLPQMERDAKAEYDAEHIPGARFFDIDEISDHRSDLPHMAPPVEKFMSRLR
AMGVGDGHQVVIYDGAGLFSAARVWWLFRLMGQVNVAVLDGGLPKWKAEGRPLEDLPPVIRDRHMTVRVQNHLVRDVTQV
SSAAKLGDHEIIDARAAERFRGEAPEPRPGMRAGHIPGSKNVPFTTLLNDDGTMKDAETLRSLFEAAGVDLTKPAITSCG
SGVTAAVLSLAMERMGKHDHALYDGSWSEWGAFPTLPVATGDN

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=272, Percent_Identity=43.0147058823529, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI61835204, Length=272, Percent_Identity=43.0147058823529, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI194473681, Length=272, Percent_Identity=43.0147058823529, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI17402865, Length=273, Percent_Identity=41.3919413919414, Blast_Score=207, Evalue=1e-53,
Organism=Escherichia coli, GI87082121, Length=273, Percent_Identity=44.6886446886447, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI87081967, Length=274, Percent_Identity=27.7372262773723, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17561888, Length=303, Percent_Identity=28.0528052805281, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71997283, Length=306, Percent_Identity=27.1241830065359, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI115534702, Length=246, Percent_Identity=26.0162601626016, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17543836, Length=276, Percent_Identity=27.8985507246377, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17543838, Length=276, Percent_Identity=27.8985507246377, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324825, Length=265, Percent_Identity=33.2075471698113, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 31181; Mature: 31181

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDPKTLVSTDWLAAHLKDPDLRILDASWYLPQMERDAKAEYDAEHIPGARFFDIDEISD
CCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHCCCCCHHHCCCCCEECHHHHHH
HRSDLPHMAPPVEKFMSRLRAMGVGDGHQVVIYDGAGLFSAARVWWLFRLMGQVNVAVLD
HHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEE
GGLPKWKAEGRPLEDLPPVIRDRHMTVRVQNHLVRDVTQVSSAAKLGDHEIIDARAAERF
CCCCCCCCCCCCHHHCCHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RGEAPEPRPGMRAGHIPGSKNVPFTTLLNDDGTMKDAETLRSLFEAAGVDLTKPAITSCG
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCC
SGVTAAVLSLAMERMGKHDHALYDGSWSEWGAFPTLPVATGDN
CCHHHHHHHHHHHHHCCCCCEEECCCCHHCCCCCCCEECCCCC
>Mature Secondary Structure
MDDPKTLVSTDWLAAHLKDPDLRILDASWYLPQMERDAKAEYDAEHIPGARFFDIDEISD
CCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHCCCCCHHHCCCCCEECHHHHHH
HRSDLPHMAPPVEKFMSRLRAMGVGDGHQVVIYDGAGLFSAARVWWLFRLMGQVNVAVLD
HHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEE
GGLPKWKAEGRPLEDLPPVIRDRHMTVRVQNHLVRDVTQVSSAAKLGDHEIIDARAAERF
CCCCCCCCCCCCHHHCCHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RGEAPEPRPGMRAGHIPGSKNVPFTTLLNDDGTMKDAETLRSLFEAAGVDLTKPAITSCG
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCC
SGVTAAVLSLAMERMGKHDHALYDGSWSEWGAFPTLPVATGDN
CCHHHHHHHHHHHHHCCCCCEEECCCCHHCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]