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The map label for this gene is trmD [H]

Identifier: 99082451

GI number: 99082451

Start: 2745980

End: 2746780

Strand: Direct

Name: trmD [H]

Synonym: TM1040_2611

Alternate gene names: 99082451

Gene position: 2745980-2746780 (Clockwise)

Preceding gene: 99082449

Following gene: 99082453

Centisome position: 85.79

GC content: 62.55

Gene sequence:

>801_bases
ATGAGCAAACCAACAAAATCCCATGGGCGCCTCTCGATCGGGGCCAGCCTCAAGCCTCGCGAGTTGATGAGCGACACGCC
GGATCTTGCGGGCGTCTGGAAAGCAAAGATCCTTACGCTCTTTCCCGAAGCCTTCCCGGGCGTTCTGGGCGAGAGCCTCA
CGGGCAAGGCATTGCAGCAAGGGCTTTGGCAGCTGGAAACCGTCGATCTGCGCCCCTTTGGGATTGGCAAGCACAAGAAC
GTCGATGACACCCCCGCCGGGGGCGGTGCGGGCATGGTGCTGCGCGCCGACGTACTGGGCGACGCCATCGAACACGCCAT
GCAGGGCACTGCCGGCAACTGGCCTCTGATTTACCTTTCCCCGCGCGGGCGGCGGATGGACCAGCAGATGATGCAAACCT
TTGCCCGGTGCGATGGGCTGACACTCCTGTGTGGACGCTTTGAGGGGGTCGATGAACGGGTGCTCGAACACTACGGCATC
CAGGAGGTCTCGCTTGGGGATTTTGTGATGACCGGTGGCGAGCTTGCGGCGCAGGCGCTCATTGATGCCACCGTACGGCT
CATTCCCGGCGTGCTGGGCAACCAGGCCTCCACCGAAGAGGAGAGCTTCTCCTCGGGTCTTCTCGAACATCCCCAATACA
CGCGTCCTGCCGACTGGAAAGGCCGCGCGATCCCCGATGTTCTGATGTCCGGCCACCATGGCAAAATCGCCGAGTGGCGG
CAGGACATGAGCGAAAAGATCACCCAAGAACGCCGCCCGGATCTGTGGGCGGCGTATCAGGCGCGCAAGTCCAAGACCTG
A

Upstream 100 bases:

>100_bases
GACGTATCGCTCATGTGCCCCCCGGGACGCTGCTCCATATGAGTAATCCCCCACAAATCCCTATTGCAAACCTTACTGAA
GGGCGGCATGCACTTTTGCC

Downstream 100 bases:

>100_bases
TCAGCAGTCCAGCTTGAGCTGCCAGCGGTTGAAACTGTCGAGCTGCTCTTGCGTGCTGCGCAGGTTGGTTGTCTCGCTGT
AGCCTTTCAGCAGATCCAGA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MSKPTKSHGRLSIGASLKPRELMSDTPDLAGVWKAKILTLFPEAFPGVLGESLTGKALQQGLWQLETVDLRPFGIGKHKN
VDDTPAGGGAGMVLRADVLGDAIEHAMQGTAGNWPLIYLSPRGRRMDQQMMQTFARCDGLTLLCGRFEGVDERVLEHYGI
QEVSLGDFVMTGGELAAQALIDATVRLIPGVLGNQASTEEESFSSGLLEHPQYTRPADWKGRAIPDVLMSGHHGKIAEWR
QDMSEKITQERRPDLWAAYQARKSKT

Sequences:

>Translated_266_residues
MSKPTKSHGRLSIGASLKPRELMSDTPDLAGVWKAKILTLFPEAFPGVLGESLTGKALQQGLWQLETVDLRPFGIGKHKN
VDDTPAGGGAGMVLRADVLGDAIEHAMQGTAGNWPLIYLSPRGRRMDQQMMQTFARCDGLTLLCGRFEGVDERVLEHYGI
QEVSLGDFVMTGGELAAQALIDATVRLIPGVLGNQASTEEESFSSGLLEHPQYTRPADWKGRAIPDVLMSGHHGKIAEWR
QDMSEKITQERRPDLWAAYQARKSKT
>Mature_265_residues
SKPTKSHGRLSIGASLKPRELMSDTPDLAGVWKAKILTLFPEAFPGVLGESLTGKALQQGLWQLETVDLRPFGIGKHKNV
DDTPAGGGAGMVLRADVLGDAIEHAMQGTAGNWPLIYLSPRGRRMDQQMMQTFARCDGLTLLCGRFEGVDERVLEHYGIQ
EVSLGDFVMTGGELAAQALIDATVRLIPGVLGNQASTEEESFSSGLLEHPQYTRPADWKGRAIPDVLMSGHHGKIAEWRQ
DMSEKITQERRPDLWAAYQARKSKT

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=219, Percent_Identity=44.7488584474886, Blast_Score=187, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 29135; Mature: 29003

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPTKSHGRLSIGASLKPRELMSDTPDLAGVWKAKILTLFPEAFPGVLGESLTGKALQQ
CCCCCCCCCCEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GLWQLETVDLRPFGIGKHKNVDDTPAGGGAGMVLRADVLGDAIEHAMQGTAGNWPLIYLS
HHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCEEEEC
PRGRRMDQQMMQTFARCDGLTLLCGRFEGVDERVLEHYGIQEVSLGDFVMTGGELAAQAL
CCCCCHHHHHHHHHHHCCCCEEEHHCCCCHHHHHHHHCCCCEECCCCCHHCCHHHHHHHH
IDATVRLIPGVLGNQASTEEESFSSGLLEHPQYTRPADWKGRAIPDVLMSGHHGKIAEWR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHH
QDMSEKITQERRPDLWAAYQARKSKT
HHHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SKPTKSHGRLSIGASLKPRELMSDTPDLAGVWKAKILTLFPEAFPGVLGESLTGKALQQ
CCCCCCCCCEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GLWQLETVDLRPFGIGKHKNVDDTPAGGGAGMVLRADVLGDAIEHAMQGTAGNWPLIYLS
HHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCEEEEC
PRGRRMDQQMMQTFARCDGLTLLCGRFEGVDERVLEHYGIQEVSLGDFVMTGGELAAQAL
CCCCCHHHHHHHHHHHCCCCEEEHHCCCCHHHHHHHHCCCCEECCCCCHHCCHHHHHHHH
IDATVRLIPGVLGNQASTEEESFSSGLLEHPQYTRPADWKGRAIPDVLMSGHHGKIAEWR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHH
QDMSEKITQERRPDLWAAYQARKSKT
HHHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA