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The map label for this gene is cobD

Identifier: 99082425

GI number: 99082425

Start: 2722447

End: 2723358

Strand: Direct

Name: cobD

Synonym: TM1040_2585

Alternate gene names: 99082425

Gene position: 2722447-2723358 (Clockwise)

Preceding gene: 99082424

Following gene: 99082426

Centisome position: 85.05

GC content: 63.71

Gene sequence:

>912_bases
ATGAGCACCGCCGCCCTGTTGATCCCCGCCATGATCCTCGACGCCGCATTTGGCGAGCCCAAATGGCTCTGGTCGCGGCT
GCCGCATCCCGCTGTGCTGATGGGCAAGCTGGTGCAAGCGCTGGATGATCGGCTGAACGAAGGTGACGGGCGCCAGGTCA
AAGGTGTTATCGCGGTCGCGGTGCTGGTATTCGTCGGTTTGCTCCTCGGCTGGATCCTGTCCTGGTTTGGCAGCCTCGTA
AGCGTTCTGATCGCCGCCATCCTGATCGCTCAACGGTCTCTTATTGACCATGTGCGCGCAGTCGCCACCGGGCTTCAGAA
TGACCTCGACGCGGGGCGCTCTGCGGTCGCGATGATCGTCAGCCGCGACACCGCGACCATGACCGGCCCGCAGATCGCGC
GCTCCGCCATCGAGAGCGGTGCGGAGAATTTCTCCGACGGGGTTATCGCGCCTGCATTCTGGTTTCTGGTCGCGGGCCTG
CCGGGGCTGCTGATCTACAAGCTCATCAACACCGCCGACAGCATGATTGGCTATCGGACCGAGGCGTACGAGGACTTCGG
CTGGGCAGCGGCGCGGCTCGACGATGTGCTCAACATCCTGCCGGCGCGCCTCAGCGCACTCCTGATCGCGCTTGTGACCG
GCCGCGCAGGTGATTGGGGCGAGATCAGCGCGGATGCACGCAAGCATCGCTCTCCCAATGCAGGCTGGCCCGAGGCAGCG
ATGGCGCGCGCACTTGGTGTCGCTCTGGCCGGGCCGCGCTCTTATGATGGCGAAATGCGTCCCTTTGCCTGGGTAAACGC
GAGCGGATCAAAAAGCGCCAGCGCTCATAGCATCACCCGCTGTTGCGAGGTGCTTTGGAAATCCTGGGGTCTCGCGCTGG
TTCTGGTGGTTGCATTGGGTCTGCTCTTCTAG

Upstream 100 bases:

>100_bases
GTGCGCACATCTGGAGCCGCATCTTTCCCTATTCAACCCGTTTCCTGCGCCTTGGCCTGCCTCCCGCAGACCGCTGGAGC
CAACTGGAGACCGCCCTCAC

Downstream 100 bases:

>100_bases
CTAACGTAGCCGCGACATCATCCGATCAAAGGACCTTCGGCCATGCGCGTGCTGCCCAGCCCCAACGCGGTCTTGCACCG
TATTGCCCTCATCACCCTCC

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSTAALLIPAMILDAAFGEPKWLWSRLPHPAVLMGKLVQALDDRLNEGDGRQVKGVIAVAVLVFVGLLLGWILSWFGSLV
SVLIAAILIAQRSLIDHVRAVATGLQNDLDAGRSAVAMIVSRDTATMTGPQIARSAIESGAENFSDGVIAPAFWFLVAGL
PGLLIYKLINTADSMIGYRTEAYEDFGWAAARLDDVLNILPARLSALLIALVTGRAGDWGEISADARKHRSPNAGWPEAA
MARALGVALAGPRSYDGEMRPFAWVNASGSKSASAHSITRCCEVLWKSWGLALVLVVALGLLF

Sequences:

>Translated_303_residues
MSTAALLIPAMILDAAFGEPKWLWSRLPHPAVLMGKLVQALDDRLNEGDGRQVKGVIAVAVLVFVGLLLGWILSWFGSLV
SVLIAAILIAQRSLIDHVRAVATGLQNDLDAGRSAVAMIVSRDTATMTGPQIARSAIESGAENFSDGVIAPAFWFLVAGL
PGLLIYKLINTADSMIGYRTEAYEDFGWAAARLDDVLNILPARLSALLIALVTGRAGDWGEISADARKHRSPNAGWPEAA
MARALGVALAGPRSYDGEMRPFAWVNASGSKSASAHSITRCCEVLWKSWGLALVLVVALGLLF
>Mature_302_residues
STAALLIPAMILDAAFGEPKWLWSRLPHPAVLMGKLVQALDDRLNEGDGRQVKGVIAVAVLVFVGLLLGWILSWFGSLVS
VLIAAILIAQRSLIDHVRAVATGLQNDLDAGRSAVAMIVSRDTATMTGPQIARSAIESGAENFSDGVIAPAFWFLVAGLP
GLLIYKLINTADSMIGYRTEAYEDFGWAAARLDDVLNILPARLSALLIALVTGRAGDWGEISADARKHRSPNAGWPEAAM
ARALGVALAGPRSYDGEMRPFAWVNASGSKSASAHSITRCCEVLWKSWGLALVLVVALGLLF

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBD_SILST (Q1GDE9)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614579.1
- STRING:   Q1GDE9
- GeneID:   4077496
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2585
- NMPDR:   fig|292414.1.peg.29
- eggNOG:   COG1270
- HOGENOM:   HBG647131
- OMA:   SKSASAH
- ProtClustDB:   PRK01209
- BioCyc:   SSP292414:TM1040_2585-MONOMER
- HAMAP:   MF_00024
- InterPro:   IPR004485
- TIGRFAMs:   TIGR00380

Pfam domain/function: PF03186 CobD_Cbib

EC number: 6.3.1.10

Molecular weight: Translated: 32241; Mature: 32110

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11ac0bb4)-; HASH(0x121a1824)-; HASH(0x12145e24)-; HASH(0x11c7e93c)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAALLIPAMILDAAFGEPKWLWSRLPHPAVLMGKLVQALDDRLNEGDGRQVKGVIAVA
CCHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VLVFVGLLLGWILSWFGSLVSVLIAAILIAQRSLIDHVRAVATGLQNDLDAGRSAVAMIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRDTATMTGPQIARSAIESGAENFSDGVIAPAFWFLVAGLPGLLIYKLINTADSMIGYRT
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
EAYEDFGWAAARLDDVLNILPARLSALLIALVTGRAGDWGEISADARKHRSPNAGWPEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCHHH
MARALGVALAGPRSYDGEMRPFAWVNASGSKSASAHSITRCCEVLWKSWGLALVLVVALG
HHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLF
HHH
>Mature Secondary Structure 
STAALLIPAMILDAAFGEPKWLWSRLPHPAVLMGKLVQALDDRLNEGDGRQVKGVIAVA
CHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VLVFVGLLLGWILSWFGSLVSVLIAAILIAQRSLIDHVRAVATGLQNDLDAGRSAVAMIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRDTATMTGPQIARSAIESGAENFSDGVIAPAFWFLVAGLPGLLIYKLINTADSMIGYRT
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
EAYEDFGWAAARLDDVLNILPARLSALLIALVTGRAGDWGEISADARKHRSPNAGWPEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCHHH
MARALGVALAGPRSYDGEMRPFAWVNASGSKSASAHSITRCCEVLWKSWGLALVLVVALG
HHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLF
HHH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA