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The map label for this gene is def [H]

Identifier: 99082419

GI number: 99082419

Start: 2717304

End: 2717813

Strand: Reverse

Name: def [H]

Synonym: TM1040_2579

Alternate gene names: 99082419

Gene position: 2717813-2717304 (Counterclockwise)

Preceding gene: 99082420

Following gene: 99082418

Centisome position: 84.91

GC content: 60.2

Gene sequence:

>510_bases
ATGAGCCTGTTGCCTATCGTCCAATGGCCGGATCCGCGCCTGTCGACGGCCTGTGCTCCGGTGGGGGCTGCCGAAGACCT
TGGCACGCTGATCGACGATGTGTTGGAGACCATGTATGCCGCACCCGGGCGCGGCCTTGCAGCGCCTCAGGTGGGGGTGC
TGAAGCGGGTGTTCGTGATGGACGTCGATTGGAAAGAGGGCCCGCGCAACCCTGTGGTGATGATATACCCCGAAGTCCTG
TGGCGCAGCGACGACACCACACTGGCCAAGGAGGCCTGCCTTTCAATCCCGGGACTTTCCACGCGGATCACCCGCCCCAC
AAAGATCCGCATACGCTGGCAGGACGCTGATCGTGCTGCGCAGGAACAAACCTTTGATGGCTTTGCTGCCCGCTGTATCC
AGCATGAATATGACCACCTTGATGGACGCGTTACATTCGATCACTTTTCCTCCGAGGAACGCCGACTTCTTGAGGCGCAG
TATCTCTCTCAAAACGAGGCGACCGCATGA

Upstream 100 bases:

>100_bases
TCGACGGTAAACTTTTCATCGATTACCTGAAGCCGCTCAAGCGCCAGATGATCACCCGCAAGATGCAAAAGCTCAAACGC
GAACGCGCGCGCGGCACCGC

Downstream 100 bases:

>100_bases
CGACCCGCACTTGCTTGCCCTGGCCCGATAAACGACTGCGCACCAAGGCTGCAGAGGTGACCGAGATCACCGATGAGATC
CGTGCCATCTGGGTCGACAT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MSLLPIVQWPDPRLSTACAPVGAAEDLGTLIDDVLETMYAAPGRGLAAPQVGVLKRVFVMDVDWKEGPRNPVVMIYPEVL
WRSDDTTLAKEACLSIPGLSTRITRPTKIRIRWQDADRAAQEQTFDGFAARCIQHEYDHLDGRVTFDHFSSEERRLLEAQ
YLSQNEATA

Sequences:

>Translated_169_residues
MSLLPIVQWPDPRLSTACAPVGAAEDLGTLIDDVLETMYAAPGRGLAAPQVGVLKRVFVMDVDWKEGPRNPVVMIYPEVL
WRSDDTTLAKEACLSIPGLSTRITRPTKIRIRWQDADRAAQEQTFDGFAARCIQHEYDHLDGRVTFDHFSSEERRLLEAQ
YLSQNEATA
>Mature_168_residues
SLLPIVQWPDPRLSTACAPVGAAEDLGTLIDDVLETMYAAPGRGLAAPQVGVLKRVFVMDVDWKEGPRNPVVMIYPEVLW
RSDDTTLAKEACLSIPGLSTRITRPTKIRIRWQDADRAAQEQTFDGFAARCIQHEYDHLDGRVTFDHFSSEERRLLEAQY
LSQNEATA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=151, Percent_Identity=41.0596026490066, Blast_Score=111, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18957; Mature: 18825

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLPIVQWPDPRLSTACAPVGAAEDLGTLIDDVLETMYAAPGRGLAAPQVGVLKRVFVM
CCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHE
DVDWKEGPRNPVVMIYPEVLWRSDDTTLAKEACLSIPGLSTRITRPTKIRIRWQDADRAA
ECCCCCCCCCCEEEEEHHHEECCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHH
QEQTFDGFAARCIQHEYDHLDGRVTFDHFSSEERRLLEAQYLSQNEATA
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SLLPIVQWPDPRLSTACAPVGAAEDLGTLIDDVLETMYAAPGRGLAAPQVGVLKRVFVM
CCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHE
DVDWKEGPRNPVVMIYPEVLWRSDDTTLAKEACLSIPGLSTRITRPTKIRIRWQDADRAA
ECCCCCCCCCCEEEEEHHHEECCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHH
QEQTFDGFAARCIQHEYDHLDGRVTFDHFSSEERRLLEAQYLSQNEATA
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA