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The map label for this gene is def [H]

Identifier: 99082418

GI number: 99082418

Start: 2716810

End: 2717307

Strand: Reverse

Name: def [H]

Synonym: TM1040_2578

Alternate gene names: 99082418

Gene position: 2717307-2716810 (Counterclockwise)

Preceding gene: 99082419

Following gene: 99082417

Centisome position: 84.89

GC content: 61.65

Gene sequence:

>498_bases
ATGACGACCCGCACTTGCTTGCCCTGGCCCGATAAACGACTGCGCACCAAGGCTGCAGAGGTGACCGAGATCACCGATGA
GATCCGTGCCATCTGGGTCGACATGATCGATACGATGGAAGCCATGCCCGGTGTTGGGCTCGCAGCGCCTCAGATCGGGG
TGATGCTGCGCCTCGCCGTCGTGGACGGCTCCAGCGAGCGTGGACGGGCCGTGCGGCTGGCCAATCCGGAAATCCTGCAC
GCCTCGATCGAACTGCGTGAACACGAAGAGGCGAGCCCGAACCTTCCCGGCGTCTCGGCCAAGATCAAACGCCCCCGTGC
GGTGACTGTGCGCTTCCTCAACGAGGATGGGGTCATCGATCGGCGCGATTTTGTTGGGATCGAGGCCACGTCGGTGCAGC
ACCAGATCGACCATCTGAACGGCAAGATGTATTTCGACCATCTGTCCAAGGTCAAACGCGACATGCTGGTCAAAAAGGCC
CGTAAGCTCGCGCGCTGA

Upstream 100 bases:

>100_bases
AATATGACCACCTTGATGGACGCGTTACATTCGATCACTTTTCCTCCGAGGAACGCCGACTTCTTGAGGCGCAGTATCTC
TCTCAAAACGAGGCGACCGC

Downstream 100 bases:

>100_bases
CGACTTCATCTTTTCTTAAATACCTCCGCCGGAAGCACCGCCTGACCCGTTGAGGGGCAGGCATATCATGTGGCGCGCAA
GCGCGTCCGCTCTGGTCGTG

Product: formylmethionine deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MTTRTCLPWPDKRLRTKAAEVTEITDEIRAIWVDMIDTMEAMPGVGLAAPQIGVMLRLAVVDGSSERGRAVRLANPEILH
ASIELREHEEASPNLPGVSAKIKRPRAVTVRFLNEDGVIDRRDFVGIEATSVQHQIDHLNGKMYFDHLSKVKRDMLVKKA
RKLAR

Sequences:

>Translated_165_residues
MTTRTCLPWPDKRLRTKAAEVTEITDEIRAIWVDMIDTMEAMPGVGLAAPQIGVMLRLAVVDGSSERGRAVRLANPEILH
ASIELREHEEASPNLPGVSAKIKRPRAVTVRFLNEDGVIDRRDFVGIEATSVQHQIDHLNGKMYFDHLSKVKRDMLVKKA
RKLAR
>Mature_164_residues
TTRTCLPWPDKRLRTKAAEVTEITDEIRAIWVDMIDTMEAMPGVGLAAPQIGVMLRLAVVDGSSERGRAVRLANPEILHA
SIELREHEEASPNLPGVSAKIKRPRAVTVRFLNEDGVIDRRDFVGIEATSVQHQIDHLNGKMYFDHLSKVKRDMLVKKAR
KLAR

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=165, Percent_Identity=38.1818181818182, Blast_Score=103, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24645728, Length=157, Percent_Identity=30.5732484076433, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24645726, Length=163, Percent_Identity=30.0613496932515, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18559; Mature: 18428

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRTCLPWPDKRLRTKAAEVTEITDEIRAIWVDMIDTMEAMPGVGLAAPQIGVMLRLAV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEE
VDGSSERGRAVRLANPEILHASIELREHEEASPNLPGVSAKIKRPRAVTVRFLNEDGVID
ECCCCCCCCEEEECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEE
RRDFVGIEATSVQHQIDHLNGKMYFDHLSKVKRDMLVKKARKLAR
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTRTCLPWPDKRLRTKAAEVTEITDEIRAIWVDMIDTMEAMPGVGLAAPQIGVMLRLAV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEE
VDGSSERGRAVRLANPEILHASIELREHEEASPNLPGVSAKIKRPRAVTVRFLNEDGVID
ECCCCCCCCEEEECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEE
RRDFVGIEATSVQHQIDHLNGKMYFDHLSKVKRDMLVKKARKLAR
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA